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The map label for this gene is cysS

Identifier: 17545886

GI number: 17545886

Start: 1224615

End: 1226012

Strand: Direct

Name: cysS

Synonym: RSc1167

Alternate gene names: 17545886

Gene position: 1224615-1226012 (Clockwise)

Preceding gene: 17545881

Following gene: 17545887

Centisome position: 32.95

GC content: 67.38

Gene sequence:

>1398_bases
ATGGAATCACTCAAGATCTACAACACGCTCGCGCGTGAGAAACAGACGTTCGTCCCCATCGAGCCCGGCCGCGTGCGCAT
GTACGTGTGCGGGATGACGGTGTACGACTACTGTCACGTCGGGCATGCGCGGGTGATGGTGGTGTTCGACATGGTGCAGC
GCTGGCTGCGCGCGTCGGGGTATGACGTGACCTACGTGCGCAACATCACCGACATCGACGACAAGATCATCAAGCGCGCG
GTCGAGAACGGCGAGACCATCGGCGCGCTCACCAACCGCTTCATCGCCGCCATGCACGAAGACGCCGACGCGCTCGGCGT
GCAGCGCCCCGACCGCGAGCCGCGCGCCACCGAATACGTGCCGCAGATGCTGTCGATGATCGGCAGGCTGCAAGACAACG
GTCTGGCCTACCAGGCGGCCGACGGCGACGTGAACTACGCGGTGCGCAAGTTCCCGGGCTACGGCAAGCTGTCGGGCAAG
TCCCTCGAAGACCTGCGCGCCGGAGAACGCGTGGCCGCCAACGATGCCAAGCAGGATCCGCTGGACTTCGTGCTGTGGAA
ATCCGCCAAGGCCGACGAGCCGGCCGAGAGTCGCTGGGCCTCGCCGTGGGGCGAGGGCCGTCCGGGCTGGCACATCGAGT
GCTCGGCGATGAGCTGCGACCTGCTCGGCACGCATTTCGACCTGCACGGCGGCGGCGCCGACCTGCAGTTCCCGCACCAC
GAAAACGAGATCGCCCAGTCCGAGGGCGCCAGCGGCAGCACCTTCGTCAACACCTGGATGCACAACGGCTTCGTGCGCGT
GAACGACGAGAAGATGTCGAAGTCGCTCGGCAACTTCTTCACCATCCGTGACGTGCTCAAGGTGTACGACCCGGAAGTCG
TGCGCTTCTTCATCCTGCGCTCGCACTACCGCAGCGACCTGAACTACAGCGATGTGCACCTGGACGATGCGCGCCACGCG
CTGACGCGCCTGTACACCGCGCTCAAGGACGTGCCCGCCGAGGCGCAGGCCCGGCCCGACTGGAACGAGCCCCATGGCAA
GCGTTTCCGCGAGGCGATGCACGACGACTTCAACACGCCCGTCGCCATGGCCGTGCTGTTCGAGCTGGCGACCGAAGTCA
ACAAGACGCGCTCACCGGCGCTGGCCAGCCAGCTGGCGGCGCTGGGCGGCGTGATGGGCCTGCTGGTCCGCGATCCGCAT
GCCTTCCTGCAGGGCGGCGTCGGCGCGGCCGCCGATGGTCTGGATGCCGCCGAGGTGGAGGCGCGCATCGAAGCCCGCCG
CGCCGCCAAGGCCGCGCGTGATTTCGCACGCGCCGACGGCATCCGCGCCGATCTGCTGGCGGCCGGCATCGTGCTCGAAG
ACAAGCCGGGCGGCGTGACCGAATGGAGACGCGCATGA

Upstream 100 bases:

>100_bases
GATATGCCGCTGATTTTGCTTCGCGCCTGCGCAGGCAGCCACGCCTGCCGGCCGACACGACGCCCCGCACGCGGCGGCGC
GCCGCTTCTCCCTACACCGC

Downstream 100 bases:

>100_bases
ACGCGGTGGCCAAGAAAGCCGGTGTGGCGCGCGCTGCGACCGTCCGCAAGGCGGCGCCGGCCAAGGCGCCCGCCGCCAAG
AAAGCGGCGGCCAAGCGCGT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MESLKIYNTLAREKQTFVPIEPGRVRMYVCGMTVYDYCHVGHARVMVVFDMVQRWLRASGYDVTYVRNITDIDDKIIKRA
VENGETIGALTNRFIAAMHEDADALGVQRPDREPRATEYVPQMLSMIGRLQDNGLAYQAADGDVNYAVRKFPGYGKLSGK
SLEDLRAGERVAANDAKQDPLDFVLWKSAKADEPAESRWASPWGEGRPGWHIECSAMSCDLLGTHFDLHGGGADLQFPHH
ENEIAQSEGASGSTFVNTWMHNGFVRVNDEKMSKSLGNFFTIRDVLKVYDPEVVRFFILRSHYRSDLNYSDVHLDDARHA
LTRLYTALKDVPAEAQARPDWNEPHGKRFREAMHDDFNTPVAMAVLFELATEVNKTRSPALASQLAALGGVMGLLVRDPH
AFLQGGVGAAADGLDAAEVEARIEARRAAKAARDFARADGIRADLLAAGIVLEDKPGGVTEWRRA

Sequences:

>Translated_465_residues
MESLKIYNTLAREKQTFVPIEPGRVRMYVCGMTVYDYCHVGHARVMVVFDMVQRWLRASGYDVTYVRNITDIDDKIIKRA
VENGETIGALTNRFIAAMHEDADALGVQRPDREPRATEYVPQMLSMIGRLQDNGLAYQAADGDVNYAVRKFPGYGKLSGK
SLEDLRAGERVAANDAKQDPLDFVLWKSAKADEPAESRWASPWGEGRPGWHIECSAMSCDLLGTHFDLHGGGADLQFPHH
ENEIAQSEGASGSTFVNTWMHNGFVRVNDEKMSKSLGNFFTIRDVLKVYDPEVVRFFILRSHYRSDLNYSDVHLDDARHA
LTRLYTALKDVPAEAQARPDWNEPHGKRFREAMHDDFNTPVAMAVLFELATEVNKTRSPALASQLAALGGVMGLLVRDPH
AFLQGGVGAAADGLDAAEVEARIEARRAAKAARDFARADGIRADLLAAGIVLEDKPGGVTEWRRA
>Mature_465_residues
MESLKIYNTLAREKQTFVPIEPGRVRMYVCGMTVYDYCHVGHARVMVVFDMVQRWLRASGYDVTYVRNITDIDDKIIKRA
VENGETIGALTNRFIAAMHEDADALGVQRPDREPRATEYVPQMLSMIGRLQDNGLAYQAADGDVNYAVRKFPGYGKLSGK
SLEDLRAGERVAANDAKQDPLDFVLWKSAKADEPAESRWASPWGEGRPGWHIECSAMSCDLLGTHFDLHGGGADLQFPHH
ENEIAQSEGASGSTFVNTWMHNGFVRVNDEKMSKSLGNFFTIRDVLKVYDPEVVRFFILRSHYRSDLNYSDVHLDDARHA
LTRLYTALKDVPAEAQARPDWNEPHGKRFREAMHDDFNTPVAMAVLFELATEVNKTRSPALASQLAALGGVMGLLVRDPH
AFLQGGVGAAADGLDAAEVEARIEARRAAKAARDFARADGIRADLLAAGIVLEDKPGGVTEWRRA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=515, Percent_Identity=35.3398058252427, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI21269877, Length=280, Percent_Identity=37.1428571428571, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI303304987, Length=280, Percent_Identity=37.1428571428571, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI62240992, Length=280, Percent_Identity=37.1428571428571, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI10835051, Length=280, Percent_Identity=37.1428571428571, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1786737, Length=467, Percent_Identity=56.1027837259101, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17509795, Length=236, Percent_Identity=39.8305084745763, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17509797, Length=236, Percent_Identity=39.8305084745763, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324082, Length=440, Percent_Identity=30.4545454545455, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI19922218, Length=389, Percent_Identity=36.7609254498715, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI19922364, Length=279, Percent_Identity=38.3512544802867, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_RALSO (Q8Y077)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519288.1
- ProteinModelPortal:   Q8Y077
- SMR:   Q8Y077
- GeneID:   1219982
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1167
- NMPDR:   fig|267608.1.peg.1167
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   RSOL267608:RSC1167-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51678; Mature: 51678

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLKIYNTLAREKQTFVPIEPGRVRMYVCGMTVYDYCHVGHARVMVVFDMVQRWLRASG
CCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHCCC
YDVTYVRNITDIDDKIIKRAVENGETIGALTNRFIAAMHEDADALGVQRPDREPRATEYV
CCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHH
PQMLSMIGRLQDNGLAYQAADGDVNYAVRKFPGYGKLSGKSLEDLRAGERVAANDAKQDP
HHHHHHHHHHCCCCEEEEECCCCCCHHHEECCCCCCCCCCCHHHHHCCCCHHCCCCCCCC
LDFVLWKSAKADEPAESRWASPWGEGRPGWHIECSAMSCDLLGTHFDLHGGGADLQFPHH
CCEEEECCCCCCCCHHHHCCCCCCCCCCCCEEEECCEEEEEECCEEEECCCCCCCCCCCC
ENEIAQSEGASGSTFVNTWMHNGFVRVNDEKMSKSLGNFFTIRDVLKVYDPEVVRFFILR
CHHHHHHCCCCCCHHHHHHHHCCEEEECHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHH
SHYRSDLNYSDVHLDDARHALTRLYTALKDVPAEAQARPDWNEPHGKRFREAMHDDFNTP
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
VAMAVLFELATEVNKTRSPALASQLAALGGVMGLLVRDPHAFLQGGVGAAADGLDAAEVE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
ARIEARRAAKAARDFARADGIRADLLAAGIVLEDKPGGVTEWRRA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCCCHHCCC
>Mature Secondary Structure
MESLKIYNTLAREKQTFVPIEPGRVRMYVCGMTVYDYCHVGHARVMVVFDMVQRWLRASG
CCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHCCC
YDVTYVRNITDIDDKIIKRAVENGETIGALTNRFIAAMHEDADALGVQRPDREPRATEYV
CCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHH
PQMLSMIGRLQDNGLAYQAADGDVNYAVRKFPGYGKLSGKSLEDLRAGERVAANDAKQDP
HHHHHHHHHHCCCCEEEEECCCCCCHHHEECCCCCCCCCCCHHHHHCCCCHHCCCCCCCC
LDFVLWKSAKADEPAESRWASPWGEGRPGWHIECSAMSCDLLGTHFDLHGGGADLQFPHH
CCEEEECCCCCCCCHHHHCCCCCCCCCCCCEEEECCEEEEEECCEEEECCCCCCCCCCCC
ENEIAQSEGASGSTFVNTWMHNGFVRVNDEKMSKSLGNFFTIRDVLKVYDPEVVRFFILR
CHHHHHHCCCCCCHHHHHHHHCCEEEECHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHH
SHYRSDLNYSDVHLDDARHALTRLYTALKDVPAEAQARPDWNEPHGKRFREAMHDDFNTP
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
VAMAVLFELATEVNKTRSPALASQLAALGGVMGLLVRDPHAFLQGGVGAAADGLDAAEVE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
ARIEARRAAKAARDFARADGIRADLLAAGIVLEDKPGGVTEWRRA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852