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The map label for this gene is yafC [H]

Identifier: 17545871

GI number: 17545871

Start: 1209960

End: 1210853

Strand: Reverse

Name: yafC [H]

Synonym: RSc1152

Alternate gene names: 17545871

Gene position: 1210853-1209960 (Counterclockwise)

Preceding gene: 17545876

Following gene: 17545870

Centisome position: 32.58

GC content: 70.69

Gene sequence:

>894_bases
ATGAAAACGACGCTCGAAGAACTGCTCGCCTTCCGCACGGTGGTCGACACCGGCTCGGTCACCGCCGCCGCCGAACAGCT
CGGCCAGACCGTCTCGGGTGTGAGCCGCGCACTGCGCCGGCTGGAAGAGAAGCTGGAGACCACGCTGCTGTCCCGCACCA
CGCGCCGGCTGGCGCTGACCGACGAGGGCACGGCCTTCCTGGCGCACGCGCGCGGCATCCTCGCCGCCGTCGACGAAGCC
GAGGAGCTGATCGCCCTGCGCCGCCAGCAGCCGGCCGGGCGCCTGCGCGTCAACGCCGCCATGCCGTTCATGCTGCATGT
GATCGCGCCGCTGGTGGCCGACTTCCGCGCGCAGTATCCGCAGATCGAGCTGGAACTGAACACCAGCGACCGCATCATCG
ACCTGCTGGAAGAAGACACCGACGTCGCCATCCGCATCGGCGCGCTGCGCGACTCGACCCTGCGAGCGCGGCGGCTCGGC
ACGCGGCGGCTGCGCGTGCTGGCCAGTCCCGCCTACCTGAAGGCGCACGGCCGTCCGCGCACGGTCGATGCGCTCCGGCA
TCACGCGCTGCTCGGCTTCGTGCAGCCCGAATCGCTGAACCACTGGCCGCTGCGCGGCCCGCACGGCGACCGCCTGCGCA
TCACGCCGACGCTCAAGGCCTCCAGCGGCGAGACCCTGCGACAACTCGCGCTGCAGGGCGCCGGCATCGTCTGCCTGGCC
GATTTCATGACGGCCCGGGATCGCCGCGCGGGCGATCTCGTCCAGGTGCTGCCCGAGGCCACCGTCGAGATCCAGCAGCC
GATCCATGCGGTCTACTACCGCAACACGCAGCTGGCCTCGCGCATCACGTGCTTTCTCGACGTGCTCGAAGCCCGCCTGG
CACAGGGCGAATGA

Upstream 100 bases:

>100_bases
GATGCAGACGGACGGAGTGTCAGGGCGCTCGCCTTTGCAAAAAACCCCGTATTGGGCAGAATACTTTTGACTAAAAATTG
ATAATCTGGCCGACGCCTCC

Downstream 100 bases:

>100_bases
GGTGAAACCCCTGCGCGGCTGTGCAACAATTGCGCGATTGCACACCCACCTTCCGTGCGCGGCCGATCGGTCGCGCGCTA
CGCATTTGACATCGCATGGC

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKTTLEELLAFRTVVDTGSVTAAAEQLGQTVSGVSRALRRLEEKLETTLLSRTTRRLALTDEGTAFLAHARGILAAVDEA
EELIALRRQQPAGRLRVNAAMPFMLHVIAPLVADFRAQYPQIELELNTSDRIIDLLEEDTDVAIRIGALRDSTLRARRLG
TRRLRVLASPAYLKAHGRPRTVDALRHHALLGFVQPESLNHWPLRGPHGDRLRITPTLKASSGETLRQLALQGAGIVCLA
DFMTARDRRAGDLVQVLPEATVEIQQPIHAVYYRNTQLASRITCFLDVLEARLAQGE

Sequences:

>Translated_297_residues
MKTTLEELLAFRTVVDTGSVTAAAEQLGQTVSGVSRALRRLEEKLETTLLSRTTRRLALTDEGTAFLAHARGILAAVDEA
EELIALRRQQPAGRLRVNAAMPFMLHVIAPLVADFRAQYPQIELELNTSDRIIDLLEEDTDVAIRIGALRDSTLRARRLG
TRRLRVLASPAYLKAHGRPRTVDALRHHALLGFVQPESLNHWPLRGPHGDRLRITPTLKASSGETLRQLALQGAGIVCLA
DFMTARDRRAGDLVQVLPEATVEIQQPIHAVYYRNTQLASRITCFLDVLEARLAQGE
>Mature_297_residues
MKTTLEELLAFRTVVDTGSVTAAAEQLGQTVSGVSRALRRLEEKLETTLLSRTTRRLALTDEGTAFLAHARGILAAVDEA
EELIALRRQQPAGRLRVNAAMPFMLHVIAPLVADFRAQYPQIELELNTSDRIIDLLEEDTDVAIRIGALRDSTLRARRLG
TRRLRVLASPAYLKAHGRPRTVDALRHHALLGFVQPESLNHWPLRGPHGDRLRITPTLKASSGETLRQLALQGAGIVCLA
DFMTARDRRAGDLVQVLPEATVEIQQPIHAVYYRNTQLASRITCFLDVLEARLAQGE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=44.636678200692, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=31.25, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=33.1103678929766, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789440, Length=272, Percent_Identity=31.25, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=30.6338028169014, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=26.2626262626263, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=27.1186440677966, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789173, Length=240, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=27.3648648648649, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082132, Length=257, Percent_Identity=24.124513618677, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788481, Length=305, Percent_Identity=24.5901639344262, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=33.1210191082803, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=187, Percent_Identity=26.7379679144385, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=265, Percent_Identity=21.5094339622642, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790011, Length=274, Percent_Identity=26.2773722627737, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787806, Length=153, Percent_Identity=30.718954248366, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=159, Percent_Identity=32.0754716981132, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32921; Mature: 32921

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTLEELLAFRTVVDTGSVTAAAEQLGQTVSGVSRALRRLEEKLETTLLSRTTRRLALT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DEGTAFLAHARGILAAVDEAEELIALRRQQPAGRLRVNAAMPFMLHVIAPLVADFRAQYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCC
QIELELNTSDRIIDLLEEDTDVAIRIGALRDSTLRARRLGTRRLRVLASPAYLKAHGRPR
EEEEEECCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCC
TVDALRHHALLGFVQPESLNHWPLRGPHGDRLRITPTLKASSGETLRQLALQGAGIVCLA
HHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCEEHH
DFMTARDRRAGDLVQVLPEATVEIQQPIHAVYYRNTQLASRITCFLDVLEARLAQGE
HHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTLEELLAFRTVVDTGSVTAAAEQLGQTVSGVSRALRRLEEKLETTLLSRTTRRLALT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DEGTAFLAHARGILAAVDEAEELIALRRQQPAGRLRVNAAMPFMLHVIAPLVADFRAQYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCC
QIELELNTSDRIIDLLEEDTDVAIRIGALRDSTLRARRLGTRRLRVLASPAYLKAHGRPR
EEEEEECCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCC
TVDALRHHALLGFVQPESLNHWPLRGPHGDRLRITPTLKASSGETLRQLALQGAGIVCLA
HHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCEEHH
DFMTARDRRAGDLVQVLPEATVEIQQPIHAVYYRNTQLASRITCFLDVLEARLAQGE
HHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]