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The map label for this gene is dapA

Identifier: 17545864

GI number: 17545864

Start: 1200763

End: 1201647

Strand: Direct

Name: dapA

Synonym: RSc1145

Alternate gene names: 17545864

Gene position: 1200763-1201647 (Clockwise)

Preceding gene: 17545863

Following gene: 17545865

Centisome position: 32.31

GC content: 65.76

Gene sequence:

>885_bases
ATGACCCAGATTTCCGGCAGTCTCGTCGCCATCGTCACGCCCATGCACGAGGACGGCAGCCTAGATTTCCCGTCGTTGCG
CAGCCTGATCGACTGGCATATCGCCGAAGGCACGGACGGCATCGTCATCGTCGGGACCAGCGGTGAATCGCCGACGGTGT
CGGTGGACGAGCACCGGGAGCTGATCCGCGTGACGGTCGAGCAGGTCAACAAGCGCATTCCGGTCATTGCCGGCACCGGC
GGCAATTCCACCACCGAAGCCGTGGAGCTGACGGCCTACGCCAAGTCGGTGGGCGCCGATGCCTCGCTGCAGGTCGTGCC
GTACTACAACAAGCCGACGCAGGAGGGCATGTACCTGCACTTCCGCAAGGTGGCGGAGTCGGTGGACCTGCCGGTGATCC
TGTACAACGTGCCTGGCCGCACCGTCGCCGATATGTCGGTCGACACGATGCTGCGCCTCGCGCAGGTGCCCGGCGTGATG
GGCGTCAAGGAGGCGACCGGCAATATCGACCGTGCCGCCCAGTTGATCAAGGCCGCGCCGGCGTCCTTCAAGATTTACAG
CGGGGACGACCCGACCGCCGTCGCGCTGATGCTGCTGGGCGGTCACGGCAACATCTCCGTGACGGCCAACGTGGCGCCGC
GCGCCATGCATGACTTGTGCGCCGCCGCCATGCGCGGCGATGTGGAGACGGCCCGCCGCATTCACATGCAGCTGCTGTCG
GTGCACAAGAACCTGTTCGTGGAATCGAACCCGATTCCCGTCAAGTGGGCCCTGCAAGCCATGGGCAAGATGGCCGGCGG
CATCCGCCTGCCGTTGACGCCGCTGGCGGCCCAGTATCAAGAGGTGGTGCGCGCATCCTTGCAGGATGCCGGACTGCTCT
CCTGA

Upstream 100 bases:

>100_bases
GTCCCCGCCTTGTGCAGGGCTTCGCGTGTGGCGAATTCATTTTTACGATCCGGTACAATCCCACTGATTTACGTTTACGC
TTTTGTCTGCTTTCGAAACC

Downstream 100 bases:

>100_bases
TTCATAAAGCCCTCGTTCTCATCTGAAGGATCGCAAGTCAATGAAACTGCTCCAAACCCGTCGCGGGGCTGTGGCGACGT
CCGCTCAACTGCTGCTGGCC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTQISGSLVAIVTPMHEDGSLDFPSLRSLIDWHIAEGTDGIVIVGTSGESPTVSVDEHRELIRVTVEQVNKRIPVIAGTG
GNSTTEAVELTAYAKSVGADASLQVVPYYNKPTQEGMYLHFRKVAESVDLPVILYNVPGRTVADMSVDTMLRLAQVPGVM
GVKEATGNIDRAAQLIKAAPASFKIYSGDDPTAVALMLLGGHGNISVTANVAPRAMHDLCAAAMRGDVETARRIHMQLLS
VHKNLFVESNPIPVKWALQAMGKMAGGIRLPLTPLAAQYQEVVRASLQDAGLLS

Sequences:

>Translated_294_residues
MTQISGSLVAIVTPMHEDGSLDFPSLRSLIDWHIAEGTDGIVIVGTSGESPTVSVDEHRELIRVTVEQVNKRIPVIAGTG
GNSTTEAVELTAYAKSVGADASLQVVPYYNKPTQEGMYLHFRKVAESVDLPVILYNVPGRTVADMSVDTMLRLAQVPGVM
GVKEATGNIDRAAQLIKAAPASFKIYSGDDPTAVALMLLGGHGNISVTANVAPRAMHDLCAAAMRGDVETARRIHMQLLS
VHKNLFVESNPIPVKWALQAMGKMAGGIRLPLTPLAAQYQEVVRASLQDAGLLS
>Mature_293_residues
TQISGSLVAIVTPMHEDGSLDFPSLRSLIDWHIAEGTDGIVIVGTSGESPTVSVDEHRELIRVTVEQVNKRIPVIAGTGG
NSTTEAVELTAYAKSVGADASLQVVPYYNKPTQEGMYLHFRKVAESVDLPVILYNVPGRTVADMSVDTMLRLAQVPGVMG
VKEATGNIDRAAQLIKAAPASFKIYSGDDPTAVALMLLGGHGNISVTANVAPRAMHDLCAAAMRGDVETARRIHMQLLSV
HKNLFVESNPIPVKWALQAMGKMAGGIRLPLTPLAAQYQEVVRASLQDAGLLS

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=293, Percent_Identity=26.962457337884, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI13540533, Length=304, Percent_Identity=24.3421052631579, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788823, Length=291, Percent_Identity=50.8591065292096, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI87082415, Length=267, Percent_Identity=32.9588014981273, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786463, Length=245, Percent_Identity=34.2857142857143, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789620, Length=241, Percent_Identity=29.8755186721992, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_RALSO (Q8Y099)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519266.1
- ProteinModelPortal:   Q8Y099
- SMR:   Q8Y099
- GeneID:   1219960
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1145
- NMPDR:   fig|267608.1.peg.1145
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- ProtClustDB:   PRK03170
- BioCyc:   RSOL267608:RSC1145-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31317; Mature: 31185

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 163-163 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQISGSLVAIVTPMHEDGSLDFPSLRSLIDWHIAEGTDGIVIVGTSGESPTVSVDEHRE
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEHHHHHH
LIRVTVEQVNKRIPVIAGTGGNSTTEAVELTAYAKSVGADASLQVVPYYNKPTQEGMYLH
HHHHHHHHHCCCCCEEEECCCCCCHHHEEHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
FRKVAESVDLPVILYNVPGRTVADMSVDTMLRLAQVPGVMGVKEATGNIDRAAQLIKAAP
HHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCC
ASFKIYSGDDPTAVALMLLGGHGNISVTANVAPRAMHDLCAAAMRGDVETARRIHMQLLS
CCEEEECCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VHKNLFVESNPIPVKWALQAMGKMAGGIRLPLTPLAAQYQEVVRASLQDAGLLS
HHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQISGSLVAIVTPMHEDGSLDFPSLRSLIDWHIAEGTDGIVIVGTSGESPTVSVDEHRE
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEHHHHHH
LIRVTVEQVNKRIPVIAGTGGNSTTEAVELTAYAKSVGADASLQVVPYYNKPTQEGMYLH
HHHHHHHHHCCCCCEEEECCCCCCHHHEEHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
FRKVAESVDLPVILYNVPGRTVADMSVDTMLRLAQVPGVMGVKEATGNIDRAAQLIKAAP
HHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCC
ASFKIYSGDDPTAVALMLLGGHGNISVTANVAPRAMHDLCAAAMRGDVETARRIHMQLLS
CCEEEECCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VHKNLFVESNPIPVKWALQAMGKMAGGIRLPLTPLAAQYQEVVRASLQDAGLLS
HHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852