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The map label for this gene is prfB [H]

Identifier: 17545749

GI number: 17545749

Start: 1081940

End: 1082842

Strand: Reverse

Name: prfB [H]

Synonym: RSc1030

Alternate gene names: 17545749

Gene position: 1082842-1081940 (Counterclockwise)

Preceding gene: 17545755

Following gene: 17545748

Centisome position: 29.14

GC content: 64.67

Gene sequence:

>903_bases
GTGGTCTTTGCGCTGACCAAGCTCGACGAAGACCTCACCGGCGGCGCCGAGCTGTTCGAACTGGCTCGCGAAGAGGGCGA
TGACGACACCATCGAGGCCGTCGAGGCCGATACCGCAGGCATGCGCGCCATTGTCGAAGACATGGAATTCCGCCGCATGT
TCTCCGGCCCGATGGACGCCGCCAACTGCTTCATCGACATCCAGGCCGGCGCCGGCGGCACCGAGGCGTGCGACTGGGCC
TCGATGCTGCTGCGCCAATACCTGAAGTACTGCGAACGCAAGGGCTTCAAGACCGAGGTGCTGGAAGAGTCCGAAGGCGA
TGTCGCCGGCATCAAGAGCGCCTCGCTCAAGGTCGAGGGCGAATACGCCTTTGGCTTCCTGCGGACCGAAACCGGCGTGC
ACCGCCTGGTGCGCAAGTCGCCGTTCGACTCGGCGGGCGGGCGCCATACGTCGTTCTCGTCGATCTTCGTGTATCCGGAA
GTGGACGACTCGATCGAGATCGAGGTCAACCCGGCGGATCTGCGCGTCGACACGTACCGCGCGTCCGGCGCGGGCGGCCA
GCACATCAACAAGACCGACTCGGCCGTGCGGATCACGCACATCCCGACCGGCATCGTCGTGCAGTGCCAGAACGACCGCT
CGCAGCACCGCAACCGCGCCGAAGCCATGACGATGCTGAAGTCGCGCCTGTACGAGCATGAGCTGCGCAAGCGCCAGGCC
GAGGCCGACAAGCTGGAGGCCTCCAAGACCGACGTGGGCTGGGGCCACCAGATCCGTTCCTACGTGCTGGACCAGAGCCG
CATCAAGGATCTGCGCACCAACGTGGAAATCTCCAACACCCAGAAAGTGCTGGACGGCGACCTCGATGCCTTCATCGAAG
CCAGCCTGAAGCAAGGCGTCTGA

Upstream 100 bases:

>100_bases
ACGTCTGACCGAAGTCGACAAAGAGCTCGAAAACCCCGAGGTCTGGAACGACCCGAAACGTGCGCAGGAACTGGGCCGCG
AGAAGAAATCGCTCGAAGGC

Downstream 100 bases:

>100_bases
TCCGGGGCACGGGCGCATCACCATGGCGGACACGTCCCTGTTCATCCTGACCGGCGCCTCGCGCGGGCTGGGCGCCGCGC
TGGCCGAAGCGCTGCTGCAG

Product: peptide chain release factor 2 protein

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MVFALTKLDEDLTGGAELFELAREEGDDDTIEAVEADTAGMRAIVEDMEFRRMFSGPMDAANCFIDIQAGAGGTEACDWA
SMLLRQYLKYCERKGFKTEVLEESEGDVAGIKSASLKVEGEYAFGFLRTETGVHRLVRKSPFDSAGGRHTSFSSIFVYPE
VDDSIEIEVNPADLRVDTYRASGAGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMTMLKSRLYEHELRKRQA
EADKLEASKTDVGWGHQIRSYVLDQSRIKDLRTNVEISNTQKVLDGDLDAFIEASLKQGV

Sequences:

>Translated_300_residues
MVFALTKLDEDLTGGAELFELAREEGDDDTIEAVEADTAGMRAIVEDMEFRRMFSGPMDAANCFIDIQAGAGGTEACDWA
SMLLRQYLKYCERKGFKTEVLEESEGDVAGIKSASLKVEGEYAFGFLRTETGVHRLVRKSPFDSAGGRHTSFSSIFVYPE
VDDSIEIEVNPADLRVDTYRASGAGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMTMLKSRLYEHELRKRQA
EADKLEASKTDVGWGHQIRSYVLDQSRIKDLRTNVEISNTQKVLDGDLDAFIEASLKQGV
>Mature_300_residues
MVFALTKLDEDLTGGAELFELAREEGDDDTIEAVEADTAGMRAIVEDMEFRRMFSGPMDAANCFIDIQAGAGGTEACDWA
SMLLRQYLKYCERKGFKTEVLEESEGDVAGIKSASLKVEGEYAFGFLRTETGVHRLVRKSPFDSAGGRHTSFSSIFVYPE
VDDSIEIEVNPADLRVDTYRASGAGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMTMLKSRLYEHELRKRQA
EADKLEASKTDVGWGHQIRSYVLDQSRIKDLRTNVEISNTQKVLDGDLDAFIEASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=246, Percent_Identity=38.6178861788618, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI34577120, Length=252, Percent_Identity=36.1111111111111, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI166795305, Length=145, Percent_Identity=40, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI2367172, Length=291, Percent_Identity=65.979381443299, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=42.1052631578947, Blast_Score=205, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=239, Percent_Identity=33.0543933054393, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=248, Percent_Identity=37.0967741935484, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19921226, Length=213, Percent_Identity=43.6619718309859, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33369; Mature: 33369

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFALTKLDEDLTGGAELFELAREEGDDDTIEAVEADTAGMRAIVEDMEFRRMFSGPMDA
CEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
ANCFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKTEVLEESEGDVAGIKSASLKVEG
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCEEEEEC
EYAFGFLRTETGVHRLVRKSPFDSAGGRHTSFSSIFVYPEVDDSIEIEVNPADLRVDTYR
CEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEE
ASGAGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMTMLKSRLYEHELRKRQA
CCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADKLEASKTDVGWGHQIRSYVLDQSRIKDLRTNVEISNTQKVLDGDLDAFIEASLKQGV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVFALTKLDEDLTGGAELFELAREEGDDDTIEAVEADTAGMRAIVEDMEFRRMFSGPMDA
CEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
ANCFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKTEVLEESEGDVAGIKSASLKVEG
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCEEEEEC
EYAFGFLRTETGVHRLVRKSPFDSAGGRHTSFSSIFVYPEVDDSIEIEVNPADLRVDTYR
CEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEE
ASGAGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMTMLKSRLYEHELRKRQA
CCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADKLEASKTDVGWGHQIRSYVLDQSRIKDLRTNVEISNTQKVLDGDLDAFIEASLKQGV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA