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The map label for this gene is yueD [H]

Identifier: 17545748

GI number: 17545748

Start: 1081177

End: 1081917

Strand: Reverse

Name: yueD [H]

Synonym: RSc1029

Alternate gene names: 17545748

Gene position: 1081917-1081177 (Counterclockwise)

Preceding gene: 17545749

Following gene: 17545747

Centisome position: 29.11

GC content: 74.36

Gene sequence:

>741_bases
ATGGCGGACACGTCCCTGTTCATCCTGACCGGCGCCTCGCGCGGGCTGGGCGCCGCGCTGGCCGAAGCGCTGCTGCAGCC
GGGCCATCGCCTGGTCTGCGTGGCGCGCGGCGACAACCCGGCGCTGCGCGCCCAGGCCGAACGTGCCGGCGTGACGCTGG
ACTGGCACCAATGCGACCTCGCCGACGCGCGCGCCGCCGAAGCCTGGATGGCACGCACGCTGGCCGCCCTGCCCGAACAC
AAGCGGGTGGCGCTGGTGCTCAATGCCGGCGTGGTCGAGCCGATCGGCGCGATCCAGACGCTGCATGCCGATGCGCTGCT
GCCGCACCTGCACCTGAACCTGGCCGGCCCGATGGCCATGACCGCCGCCCTGCTGCGCGGCACCGCCGGCTGGGGCGCGC
AGCGCCGCATCCTGGCGGTGTCCTCGGGCGCGGCGCGCCGGCCCGTTGCCGGCTGGGCCGCGTATTGCACCGGCAAGGCG
GGCCTGGACATGTTCGTGCGCGCCATCAATGCCGAGGGCGACGCCAGCACGCGCGCCGTGGCGCTGGCCCCGGGCGTGCT
CGACACCGACATGCAGCGCACCATCCGCAGCACCGACTTCGCCGGCGTGCAGCGCTTCCGCGATCTGCACGCACACGGCG
AGCTGGTCTCGCCGCAGGACGCCGCCCGGCGCATCGTCGCCTACCTCGCCCGGCCGGATTTCGGCGCGACCGAACTCGAC
GATCTGCGCAACATCGCCTGA

Upstream 100 bases:

>100_bases
GAAATCTCCAACACCCAGAAAGTGCTGGACGGCGACCTCGATGCCTTCATCGAAGCCAGCCTGAAGCAAGGCGTCTGATC
CGGGGCACGGGCGCATCACC

Downstream 100 bases:

>100_bases
CACGACTCTCCCTACACAATCATCATGACCGAACCGAACACCCCGGCCGCACCCACTGGACAGGCGCAAGACGACAACCA
GATCATGGCCGAGCGCCGCG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MADTSLFILTGASRGLGAALAEALLQPGHRLVCVARGDNPALRAQAERAGVTLDWHQCDLADARAAEAWMARTLAALPEH
KRVALVLNAGVVEPIGAIQTLHADALLPHLHLNLAGPMAMTAALLRGTAGWGAQRRILAVSSGAARRPVAGWAAYCTGKA
GLDMFVRAINAEGDASTRAVALAPGVLDTDMQRTIRSTDFAGVQRFRDLHAHGELVSPQDAARRIVAYLARPDFGATELD
DLRNIA

Sequences:

>Translated_246_residues
MADTSLFILTGASRGLGAALAEALLQPGHRLVCVARGDNPALRAQAERAGVTLDWHQCDLADARAAEAWMARTLAALPEH
KRVALVLNAGVVEPIGAIQTLHADALLPHLHLNLAGPMAMTAALLRGTAGWGAQRRILAVSSGAARRPVAGWAAYCTGKA
GLDMFVRAINAEGDASTRAVALAPGVLDTDMQRTIRSTDFAGVQRFRDLHAHGELVSPQDAARRIVAYLARPDFGATELD
DLRNIA
>Mature_245_residues
ADTSLFILTGASRGLGAALAEALLQPGHRLVCVARGDNPALRAQAERAGVTLDWHQCDLADARAAEAWMARTLAALPEHK
RVALVLNAGVVEPIGAIQTLHADALLPHLHLNLAGPMAMTAALLRGTAGWGAQRRILAVSSGAARRPVAGWAAYCTGKAG
LDMFVRAINAEGDASTRAVALAPGVLDTDMQRTIRSTDFAGVQRFRDLHAHGELVSPQDAARRIVAYLARPDFGATELDD
LRNIA

Specific function: Reduces benzil stereospecifically to (S)-benzoin. Can also reduces 1-phenyl-1,2-propanedione, 1,4-naphthoquinone, 1-(4- methyl-phenyl)-2-phenyl-ethane-1,2-dione, 1-(4-fluoro-phenyl)-2- phenyl-ethane-1,2-dione, methyl benzoylformate, p- nitrobenzaldehyde i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=246, Percent_Identity=34.1463414634146, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI40254992, Length=189, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=232, Percent_Identity=28.448275862069, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322226, Length=223, Percent_Identity=28.2511210762332, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI23397609, Length=235, Percent_Identity=31.063829787234, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 25956; Mature: 25825

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTSLFILTGASRGLGAALAEALLQPGHRLVCVARGDNPALRAQAERAGVTLDWHQCDL
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEEHHCCCC
ADARAAEAWMARTLAALPEHKRVALVLNAGVVEPIGAIQTLHADALLPHLHLNLAGPMAM
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCHHHH
TAALLRGTAGWGAQRRILAVSSGAARRPVAGWAAYCTGKAGLDMFVRAINAEGDASTRAV
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCEEE
ALAPGVLDTDMQRTIRSTDFAGVQRFRDLHAHGELVSPQDAARRIVAYLARPDFGATELD
EECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
DLRNIA
HHHCCC
>Mature Secondary Structure 
ADTSLFILTGASRGLGAALAEALLQPGHRLVCVARGDNPALRAQAERAGVTLDWHQCDL
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEEHHCCCC
ADARAAEAWMARTLAALPEHKRVALVLNAGVVEPIGAIQTLHADALLPHLHLNLAGPMAM
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCHHHH
TAALLRGTAGWGAQRRILAVSSGAARRPVAGWAAYCTGKAGLDMFVRAINAEGDASTRAV
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCEEE
ALAPGVLDTDMQRTIRSTDFAGVQRFRDLHAHGELVSPQDAARRIVAYLARPDFGATELD
EECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
DLRNIA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11745140; 11796169 [H]