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The map label for this gene is nth [H]

Identifier: 17545724

GI number: 17545724

Start: 1055611

End: 1056255

Strand: Reverse

Name: nth [H]

Synonym: RSc1005

Alternate gene names: 17545724

Gene position: 1056255-1055611 (Counterclockwise)

Preceding gene: 17545725

Following gene: 17545722

Centisome position: 28.42

GC content: 66.67

Gene sequence:

>645_bases
ATGAACCCAGCCAAGCGCCGCGCCCTCTTCGAAACGCTCCGCGAACACAACCCGACACCGACCACCGAGCTGGAATACAC
CACGCCGTTCGAACTGCTGATCGCCGTGCTGCTGTCGGCCCAGGCGACCGACGTGGGCGTGAACAAGGCCACGCGCAAGT
TGTTTCCGGTGGCCGATACGCCGGCCAAGATGCTGGCGCTGGGCGAGGAAGGCCTGACCGCCTACATCAAGACCATCGGC
CTGTACCGCACCAAGTGCAAGCACATCCTGCAGACCTGCCGCATCCTGCTCGACCAGTACGGCGGCCAGGTCCCGCGCGA
CCGCGCCGCGCTGGAAGCCTTGCCGGGCGTGGGCCGCAAGACCGCCAACGTGGTGCTGAACACGGCCTTCGGCGAGCCGA
CCATTGCGGTCGACACGCACATCTTCCGCGTCGCCAACCGCACCGGGCTGGCGCCGGGCAAGAACGTCCTGGAAGTCGAG
CTGAAGCTGCTGAAGGTGGTACCGGAGGAGTTCCGCCAGGACGCGCACCACTGGCTGATCCTGCACGGGCGCTACGTGTG
CAGGGCGCGCAAGCCCGAATGCTGGCACTGCGCGATCGAGCCGCTATGCGAGTTCAGGCCGAAGACGCCGGCGCCGAAAG
AATGA

Upstream 100 bases:

>100_bases
CAGATCGACGAGGCCGAGGCGCGCCGCAAGCGCATCGCCGACCTCAGCAAGCCGCCCTCGGACAAGCCCTGACCTCCCCT
CTCCCCACGATCGTCCCTCG

Downstream 100 bases:

>100_bases
AAAACGGGGGACCCAGAGTCCCCCGCTGCACGTCTGCTTCTGCGTATTGCCGGCCGGTCAGAGCACCCGGCAGGCCTCTT
CGAACGTCAGGCGCGGATTG

Product: endonuclease III protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNPAKRRALFETLREHNPTPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLFPVADTPAKMLALGEEGLTAYIKTIG
LYRTKCKHILQTCRILLDQYGGQVPRDRAALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVE
LKLLKVVPEEFRQDAHHWLILHGRYVCRARKPECWHCAIEPLCEFRPKTPAPKE

Sequences:

>Translated_214_residues
MNPAKRRALFETLREHNPTPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLFPVADTPAKMLALGEEGLTAYIKTIG
LYRTKCKHILQTCRILLDQYGGQVPRDRAALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVE
LKLLKVVPEEFRQDAHHWLILHGRYVCRARKPECWHCAIEPLCEFRPKTPAPKE
>Mature_214_residues
MNPAKRRALFETLREHNPTPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLFPVADTPAKMLALGEEGLTAYIKTIG
LYRTKCKHILQTCRILLDQYGGQVPRDRAALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVE
LKLLKVVPEEFRQDAHHWLILHGRYVCRARKPECWHCAIEPLCEFRPKTPAPKE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=73.0769230769231, Blast_Score=312, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.6666666666667, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23954; Mature: 23954

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAKRRALFETLREHNPTPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLFPVADT
CCHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
PAKMLALGEEGLTAYIKTIGLYRTKCKHILQTCRILLDQYGGQVPRDRAALEALPGVGRK
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVELKLLKVVPEEFRQDAHHWLI
HHHEEEECCCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
LHGRYVCRARKPECWHCAIEPLCEFRPKTPAPKE
EECEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNPAKRRALFETLREHNPTPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLFPVADT
CCHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
PAKMLALGEEGLTAYIKTIGLYRTKCKHILQTCRILLDQYGGQVPRDRAALEALPGVGRK
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVELKLLKVVPEEFRQDAHHWLI
HHHEEEECCCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
LHGRYVCRARKPECWHCAIEPLCEFRPKTPAPKE
EECEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]