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The map label for this gene is alaXM [H]

Identifier: 17545707

GI number: 17545707

Start: 1038894

End: 1039631

Strand: Reverse

Name: alaXM [H]

Synonym: RSc0988

Alternate gene names: 17545707

Gene position: 1039631-1038894 (Counterclockwise)

Preceding gene: 17545711

Following gene: 17545706

Centisome position: 27.97

GC content: 70.87

Gene sequence:

>738_bases
ATGCCCACGCTCAAGCGCTTTGACGAAGACGCCTATCGCACCGAGTGCGGCGCCACGGTCGTCGCCGTGCATCCCGAGGG
CATCGAACTGGACCAGACCGTGTTCTACGCCCGCGGCGGCGGCCAGGCCGGCGACACCGGCACCCTGATCCTGCCCGACG
GCCAGACCGTGGCGATCGCCGACACCGTCTACCACGCCGACCGCCTGGCCATCCTGCATGTGCCGGCCGATCCGGCCAAC
CTGGGCCTGCTGGCGCCCGGCCTGCCGGTGTCCGCCGCGATCGACTGGGCACGCCGGTACCGGCTAATGCGACTGCACAC
CTGCCTGCATCTGCTGGGCGCGCTGATCCCGGTGCCGGTCACGGGCTGCGGCATCTCGCCGGACGCCGGCCGCATCGACT
TCGACCTGCCCGAATCCACGCTCGAGCGCGAGACGCTGACCGCGCAGCTCAATGCGCTGATCGCGCGCGACACGCCGGTG
CGCATCGCCCTGATCACGCCGGAGGCCTTGGCCGCACAGCCGCAGTTGGTACGCACCATCGGCGCTGCGCCGCCGGCGGG
CGTGTCCGCCATCCGCATCGTGGAGATCGACGGCATCGACCGTCAGCCCTGCGGCGGCACCCACGTGCGCGCCACGGGCG
AGATCGGTCGCGCCACCGTCACCAAAATCGAAAAGAAGAGCCGCCAGAACCGCCGCGTGGTGGTGGCGCTGGCCGATGAG
ATCAGGAGCACGCCATGA

Upstream 100 bases:

>100_bases
AACTGTACAGAAAGCGCCACGCCAAAGTGTACAGGTACTGTACAGCCACAGTTCGCTACCATCAAGGTCGGCTTTCCGCC
CCCACGCCAGGAGCCCCACC

Downstream 100 bases:

>100_bases
CCCGGTCCACCGCCCTGCCGCTCGGCCATGCGCCCGACCACGCCGAACGCCGCGGCCTGCTGCTCGGCTTCATCGGCGTG
ACGATCTTCAGCCAGACGCT

Product: hypothetical protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPTLKRFDEDAYRTECGATVVAVHPEGIELDQTVFYARGGGQAGDTGTLILPDGQTVAIADTVYHADRLAILHVPADPAN
LGLLAPGLPVSAAIDWARRYRLMRLHTCLHLLGALIPVPVTGCGISPDAGRIDFDLPESTLERETLTAQLNALIARDTPV
RIALITPEALAAQPQLVRTIGAAPPAGVSAIRIVEIDGIDRQPCGGTHVRATGEIGRATVTKIEKKSRQNRRVVVALADE
IRSTP

Sequences:

>Translated_245_residues
MPTLKRFDEDAYRTECGATVVAVHPEGIELDQTVFYARGGGQAGDTGTLILPDGQTVAIADTVYHADRLAILHVPADPAN
LGLLAPGLPVSAAIDWARRYRLMRLHTCLHLLGALIPVPVTGCGISPDAGRIDFDLPESTLERETLTAQLNALIARDTPV
RIALITPEALAAQPQLVRTIGAAPPAGVSAIRIVEIDGIDRQPCGGTHVRATGEIGRATVTKIEKKSRQNRRVVVALADE
IRSTP
>Mature_244_residues
PTLKRFDEDAYRTECGATVVAVHPEGIELDQTVFYARGGGQAGDTGTLILPDGQTVAIADTVYHADRLAILHVPADPANL
GLLAPGLPVSAAIDWARRYRLMRLHTCLHLLGALIPVPVTGCGISPDAGRIDFDLPESTLERETLTAQLNALIARDTPVR
IALITPEALAAQPQLVRTIGAAPPAGVSAIRIVEIDGIDRQPCGGTHVRATGEIGRATVTKIEKKSRQNRRVVVALADEI
RSTP

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=215, Percent_Identity=27.4418604651163, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI217416402, Length=215, Percent_Identity=27.4418604651163, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789048, Length=200, Percent_Identity=31, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26076; Mature: 25945

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLKRFDEDAYRTECGATVVAVHPEGIELDQTVFYARGGGQAGDTGTLILPDGQTVAIA
CCCHHHCCCHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEECCCCEEEEE
DTVYHADRLAILHVPADPANLGLLAPGLPVSAAIDWARRYRLMRLHTCLHLLGALIPVPV
EEEEECCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TGCGISPDAGRIDFDLPESTLERETLTAQLNALIARDTPVRIALITPEALAAQPQLVRTI
ECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHHHCCHHHHHHH
GAAPPAGVSAIRIVEIDGIDRQPCGGTHVRATGEIGRATVTKIEKKSRQNRRVVVALADE
CCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEHH
IRSTP
HCCCC
>Mature Secondary Structure 
PTLKRFDEDAYRTECGATVVAVHPEGIELDQTVFYARGGGQAGDTGTLILPDGQTVAIA
CCHHHCCCHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEECCCCEEEEE
DTVYHADRLAILHVPADPANLGLLAPGLPVSAAIDWARRYRLMRLHTCLHLLGALIPVPV
EEEEECCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TGCGISPDAGRIDFDLPESTLERETLTAQLNALIARDTPVRIALITPEALAAQPQLVRTI
ECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEECCHHHHCCHHHHHHH
GAAPPAGVSAIRIVEIDGIDRQPCGGTHVRATGEIGRATVTKIEKKSRQNRRVVVALADE
CCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEHH
IRSTP
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]