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The map label for this gene is thyA

Identifier: 17545666

GI number: 17545666

Start: 994874

End: 995668

Strand: Reverse

Name: thyA

Synonym: RSc0947

Alternate gene names: 17545666

Gene position: 995668-994874 (Counterclockwise)

Preceding gene: 17545668

Following gene: 17545665

Centisome position: 26.79

GC content: 63.77

Gene sequence:

>795_bases
ATGCAGCCCTATCTCGACCTGATGCGCCATGTCCACGAGCATGGCACCGACAAAGCCGACCGCACCGGCACTGGCACGCG
CTCCGTGTTCGGCCATCAGATGCGCTTCGACCTGCAGCAGGGCTTTCCGCTGGTGACCACCAAGAAGGTCCACATCAAGT
CCATCGTCTATGAGCTGCTGTGGTTCCTGCAGGGCGCGACCAACGTGCGCTGGCTGCGGGAGAACGGCGTCACCATCTGG
GACGAATGGGCCGATGCGGAAGGTGAACTGGGCCCGATCTACGGCTACCAGTGGCGCTCGTGGCCGACGCCGTCCGGCGA
GCACATCGACCAGATCGCCGAACTGATCGCGCAGATCAAGCGCAATCCGGACTCGCGCCGGCTGATCGTCTCGGCCTGGA
ACGTGGCCGACATTCCGCGCATGAAGCTGCCGCCCTGCCACGCGTTCTTCCAGTTCTATGTGGCCGACGGCAAGCTGTCG
TGCCAGCTCTACCAGCGCAGCGCGGACATCTTCCTCGGCGTGCCGTTCAACATCGCCAGCTACGCGCTGCTCACGCACAT
GATCGCGCAGCAGACCGGCCTGGACGTGGGCGACTTCGTCTGGACCGGCGGCGACTGCCATCTCTACAACAACCACTTCG
AACAGGTCGAGACGCAACTCGCGCGCACGCCGCTGCCGCTGCCGAAGCTGCACATCAAGCGCAAGCCGGACAGCATCTTC
GATTACCGGTACGAAGACTTCGAGATCGTCGGCTACGAAAGCCATCCCGCGATCAAGGCGCCCGTGGCCGTATGA

Upstream 100 bases:

>100_bases
GACCGGGCCAGTTCGCCGGAGCAGCCGGTACAGTCCCCCGGCCGTGCCAGGCCGGTCACGTTACAATGCGGCTCGATTGT
TTCCGCCGCCCCGCCGCGCC

Downstream 100 bases:

>100_bases
CGCTCCTGACCCTGATCGTCGCGCGCGCGCGCAATGGCGTGATCGGCCGCGACAACACCCTGCCCTGGCGCCTGCCCGAA
GACCTGGCGCACTTCAAGCG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQPYLDLMRHVHEHGTDKADRTGTGTRSVFGHQMRFDLQQGFPLVTTKKVHIKSIVYELLWFLQGATNVRWLRENGVTIW
DEWADAEGELGPIYGYQWRSWPTPSGEHIDQIAELIAQIKRNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGLDVGDFVWTGGDCHLYNNHFEQVETQLARTPLPLPKLHIKRKPDSIF
DYRYEDFEIVGYESHPAIKAPVAV

Sequences:

>Translated_264_residues
MQPYLDLMRHVHEHGTDKADRTGTGTRSVFGHQMRFDLQQGFPLVTTKKVHIKSIVYELLWFLQGATNVRWLRENGVTIW
DEWADAEGELGPIYGYQWRSWPTPSGEHIDQIAELIAQIKRNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGLDVGDFVWTGGDCHLYNNHFEQVETQLARTPLPLPKLHIKRKPDSIF
DYRYEDFEIVGYESHPAIKAPVAV
>Mature_264_residues
MQPYLDLMRHVHEHGTDKADRTGTGTRSVFGHQMRFDLQQGFPLVTTKKVHIKSIVYELLWFLQGATNVRWLRENGVTIW
DEWADAEGELGPIYGYQWRSWPTPSGEHIDQIAELIAQIKRNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGLDVGDFVWTGGDCHLYNNHFEQVETQLARTPLPLPKLHIKRKPDSIF
DYRYEDFEIVGYESHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=52.3131672597865, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=71.2121212121212, Blast_Score=416, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=46.8085106382979, Blast_Score=259, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=228, Percent_Identity=45.1754385964912, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=53.7102473498233, Blast_Score=300, Evalue=8e-82,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_RALSO (Q8Y0U6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519068.1
- ProteinModelPortal:   Q8Y0U6
- SMR:   Q8Y0U6
- GeneID:   1219755
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0947
- NMPDR:   fig|267608.1.peg.947
- HOGENOM:   HBG588098
- OMA:   ADIPRMK
- ProtClustDB:   PRK01827
- BioCyc:   RSOL267608:RSC0947-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30381; Mature: 30381

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPYLDLMRHVHEHGTDKADRTGTGTRSVFGHQMRFDLQQGFPLVTTKKVHIKSIVYELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHH
WFLQGATNVRWLRENGVTIWDEWADAEGELGPIYGYQWRSWPTPSGEHIDQIAELIAQIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEECCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMIAQQTGLDVGDFVWTGGDCHLYNNHFEQVETQLARTPLPLPKLHIKRKPDSIF
HHHHHHHHHHHHCCCCHHEEEECCEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCHHH
DYRYEDFEIVGYESHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQPYLDLMRHVHEHGTDKADRTGTGTRSVFGHQMRFDLQQGFPLVTTKKVHIKSIVYELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHH
WFLQGATNVRWLRENGVTIWDEWADAEGELGPIYGYQWRSWPTPSGEHIDQIAELIAQIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEECCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMIAQQTGLDVGDFVWTGGDCHLYNNHFEQVETQLARTPLPLPKLHIKRKPDSIF
HHHHHHHHHHHHCCCCHHEEEECCEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCHHH
DYRYEDFEIVGYESHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852