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The map label for this gene is yneJ [H]

Identifier: 17545523

GI number: 17545523

Start: 851739

End: 852647

Strand: Reverse

Name: yneJ [H]

Synonym: RSc0804

Alternate gene names: 17545523

Gene position: 852647-851739 (Counterclockwise)

Preceding gene: 17545530

Following gene: 17545522

Centisome position: 22.94

GC content: 70.08

Gene sequence:

>909_bases
ATGGATCTGGCCTCGCTGGAAATCTTCCGCACCGTCGTCCGCGAAGGCGGCGTCACACGCGCGGCGGCGCAACTGCATCG
GGTGCAGTCCAACGTGACGACCCGCATCCGCCAGCTCGAGGCATCGCTGGGCGTGCCGCTGTTCGTGCGCGAAGGCAAGC
GCATGGTGCTGACGCCGGCCGGCCAGACCCTGTTGACCTATGCCGACGACCTCCTGCGCCTGGCCGCGGAAGCCCGGCAG
GCCGTGCAGCCGCGCGAGCCGCACGGCCGCCTGCGCATTGCCTCGATGGAAAGCACGGCCGCCAGTCGCCTGCCGACCCT
GCTGGCGTCGCTGCATCAGCGCTGGCCCAGGGTCCAGCTCGAGCTCGTCACCATGACGACGCGGCAATCGGTGCAGGCAC
TGTCCCGCTTCGAGGTCGATTGCGCCTTCATTGCCGAGACGACGTGCCTGGCCAACATGCGTTCCGCACTGACGACGCTG
CCGGCGTTTGCCGAAGAACTGGTGCTGATTGCCCACCGCGCGCATCCGCCCATCCGCCGCGCCGCCGACGTGCAGACCCC
CACCCTGCTCGGTTTCGAGCCCGGCTGCGCCTATCGCCAATTGATCGACGACTGGTTCGAGGCCGGCGGCGTGGTGCCCT
CGCGCGTACTGGAGCTGGGTTCGTACCACGCCATCATCGCGTGTGCGGCGGCGGGGATCGGCGTGGCGCTGGTACCGCGC
TCGGTGCTGGATCTGTACACCAACGCGCCGGAGATCAGCGTGCATGCGCTCGGCAATCCGGGGCGCGTCGACACGCTGCT
GGCCTGGCACCGCGACATGGCCGGGCCGGCGCTGCAGGCGCTGGTGCAGGTGCTGAGCGAGGCGCATGCCACGGCGCCGT
CACCCACCGGTGAGGCTGTCGCCGCCTAA

Upstream 100 bases:

>100_bases
TGGATCCGTACGAAGGCAATCGGCACCTCATGCTATGCAAGCCAACCCATCCTGAAAAATGAATTATATTGAACAGATCC
ATTCATATTTGAGATGCACC

Downstream 100 bases:

>100_bases
ACTGAATGCGAAGACCCCGCCGCAGGTCTAACAAGAAGCGCAAGCAAGCGCGGCAGTAACGAACGGAGGCGCGCATGCGA
CCGTCGTTGCGGATACGGCA

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDLASLEIFRTVVREGGVTRAAAQLHRVQSNVTTRIRQLEASLGVPLFVREGKRMVLTPAGQTLLTYADDLLRLAAEARQ
AVQPREPHGRLRIASMESTAASRLPTLLASLHQRWPRVQLELVTMTTRQSVQALSRFEVDCAFIAETTCLANMRSALTTL
PAFAEELVLIAHRAHPPIRRAADVQTPTLLGFEPGCAYRQLIDDWFEAGGVVPSRVLELGSYHAIIACAAAGIGVALVPR
SVLDLYTNAPEISVHALGNPGRVDTLLAWHRDMAGPALQALVQVLSEAHATAPSPTGEAVAA

Sequences:

>Translated_302_residues
MDLASLEIFRTVVREGGVTRAAAQLHRVQSNVTTRIRQLEASLGVPLFVREGKRMVLTPAGQTLLTYADDLLRLAAEARQ
AVQPREPHGRLRIASMESTAASRLPTLLASLHQRWPRVQLELVTMTTRQSVQALSRFEVDCAFIAETTCLANMRSALTTL
PAFAEELVLIAHRAHPPIRRAADVQTPTLLGFEPGCAYRQLIDDWFEAGGVVPSRVLELGSYHAIIACAAAGIGVALVPR
SVLDLYTNAPEISVHALGNPGRVDTLLAWHRDMAGPALQALVQVLSEAHATAPSPTGEAVAA
>Mature_302_residues
MDLASLEIFRTVVREGGVTRAAAQLHRVQSNVTTRIRQLEASLGVPLFVREGKRMVLTPAGQTLLTYADDLLRLAAEARQ
AVQPREPHGRLRIASMESTAASRLPTLLASLHQRWPRVQLELVTMTTRQSVQALSRFEVDCAFIAETTCLANMRSALTTL
PAFAEELVLIAHRAHPPIRRAADVQTPTLLGFEPGCAYRQLIDDWFEAGGVVPSRVLELGSYHAIIACAAAGIGVALVPR
SVLDLYTNAPEISVHALGNPGRVDTLLAWHRDMAGPALQALVQVLSEAHATAPSPTGEAVAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=37.6306620209059, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI87082132, Length=297, Percent_Identity=29.2929292929293, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788481, Length=272, Percent_Identity=29.7794117647059, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=25.8389261744966, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=31.6017316017316, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=29.9595141700405, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790208, Length=259, Percent_Identity=28.957528957529, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790262, Length=267, Percent_Identity=27.7153558052434, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=28.0487804878049, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788748, Length=263, Percent_Identity=25.4752851711027, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787530, Length=255, Percent_Identity=25.8823529411765, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787949, Length=297, Percent_Identity=24.5791245791246, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI48994962, Length=114, Percent_Identity=38.5964912280702, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=299, Percent_Identity=27.0903010033445, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=26.7206477732794, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32605; Mature: 32605

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLASLEIFRTVVREGGVTRAAAQLHRVQSNVTTRIRQLEASLGVPLFVREGKRMVLTPA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCC
GQTLLTYADDLLRLAAEARQAVQPREPHGRLRIASMESTAASRLPTLLASLHQRWPRVQL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEE
ELVTMTTRQSVQALSRFEVDCAFIAETTCLANMRSALTTLPAFAEELVLIAHRAHPPIRR
EEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AADVQTPTLLGFEPGCAYRQLIDDWFEAGGVVPSRVLELGSYHAIIACAAAGIGVALVPR
HCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHH
SVLDLYTNAPEISVHALGNPGRVDTLLAWHRDMAGPALQALVQVLSEAHATAPSPTGEAV
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
AA
CC
>Mature Secondary Structure
MDLASLEIFRTVVREGGVTRAAAQLHRVQSNVTTRIRQLEASLGVPLFVREGKRMVLTPA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCC
GQTLLTYADDLLRLAAEARQAVQPREPHGRLRIASMESTAASRLPTLLASLHQRWPRVQL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEE
ELVTMTTRQSVQALSRFEVDCAFIAETTCLANMRSALTTLPAFAEELVLIAHRAHPPIRR
EEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AADVQTPTLLGFEPGCAYRQLIDDWFEAGGVVPSRVLELGSYHAIIACAAAGIGVALVPR
HCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHH
SVLDLYTNAPEISVHALGNPGRVDTLLAWHRDMAGPALQALVQVLSEAHATAPSPTGEAV
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]