Click here to switch to the map view.

The map label for this gene is ribE [H]

Identifier: 17545433

GI number: 17545433

Start: 761850

End: 762503

Strand: Reverse

Name: ribE [H]

Synonym: RSc0714

Alternate gene names: 17545433

Gene position: 762503-761850 (Counterclockwise)

Preceding gene: 17545434

Following gene: 229259638

Centisome position: 20.52

GC content: 68.2

Gene sequence:

>654_bases
ATGTTTACCGGAATCGTCGCCGCCATCGGCCGCATTGAAACCGTCGAGCCGCTCGCCGCCGACGCACAGGCCGGCGTGCG
CCTGGGCATCCATGCGGGCGGCCTGCCGCTGGCCGATGTGGCGCTCGGCGACTCGATCGCCATCCAGGGCGCCTGCATGA
CGGTCGTCGCCAAGAACGACACCCACTTCGACGTGGACGTCTCGCGCGAATCGCTGTCCAAGACGGTCGGCCTGGACCGC
CCGGGCGAAGTCAACCTGGAAAAGGCATTGCGCCTGGCCGACCACATCGGCGGGCACCTGGTCTCGGGCCACGTGGACGG
CCTGGGCACCGTCAGCCATTTCGCGCCGGTGGGCGAGTCATACGAACTGCGGGTGCGCACCTCGCCGGAACTCGGCCGCT
ACTTCGCCTACAAGGGCTCGGTGGTGGTCAACGGCGTGTCGCTGACGGTCAACAGCGTGCGCGACGATGCCACCGGCTGC
GAGTTCTCGATCAACCTGATCCCGCACACGCTGGCCGTCACCACGCTCAAGCATCTGGCGGTGGGCGGCCAGGTCAATCT
GGAAATCGACCTGATCGCCCGCTACGTCGAGCGGATGCTGACCCATACGGCGACCGGCCTGCCTGCCGCGGCCCGTCCGC
TGGCCGCCGTTTGA

Upstream 100 bases:

>100_bases
CGCTGGAAGGCGCGGCGGCATTCTTCTTTCACGACATCACGCGCGTCGGCGGCGATCTCCGCATCCTGGCGCGCCGCAAC
CCGACGGATTGATCGCACGC

Downstream 100 bases:

>100_bases
CATCCCGGCCCCCGGGCCGCCTTCGCGTACAATAATGGGTTCCCATTTTTGCCGTCGCGCGGCCGCCAACGCGCTGGCCG
GCGGCCCGCTCCAGTTACCC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MFTGIVAAIGRIETVEPLAADAQAGVRLGIHAGGLPLADVALGDSIAIQGACMTVVAKNDTHFDVDVSRESLSKTVGLDR
PGEVNLEKALRLADHIGGHLVSGHVDGLGTVSHFAPVGESYELRVRTSPELGRYFAYKGSVVVNGVSLTVNSVRDDATGC
EFSINLIPHTLAVTTLKHLAVGGQVNLEIDLIARYVERMLTHTATGLPAAARPLAAV

Sequences:

>Translated_217_residues
MFTGIVAAIGRIETVEPLAADAQAGVRLGIHAGGLPLADVALGDSIAIQGACMTVVAKNDTHFDVDVSRESLSKTVGLDR
PGEVNLEKALRLADHIGGHLVSGHVDGLGTVSHFAPVGESYELRVRTSPELGRYFAYKGSVVVNGVSLTVNSVRDDATGC
EFSINLIPHTLAVTTLKHLAVGGQVNLEIDLIARYVERMLTHTATGLPAAARPLAAV
>Mature_217_residues
MFTGIVAAIGRIETVEPLAADAQAGVRLGIHAGGLPLADVALGDSIAIQGACMTVVAKNDTHFDVDVSRESLSKTVGLDR
PGEVNLEKALRLADHIGGHLVSGHVDGLGTVSHFAPVGESYELRVRTSPELGRYFAYKGSVVVNGVSLTVNSVRDDATGC
EFSINLIPHTLAVTTLKHLAVGGQVNLEIDLIARYVERMLTHTATGLPAAARPLAAV

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=210, Percent_Identity=34.2857142857143, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319733, Length=208, Percent_Identity=33.1730769230769, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22663; Mature: 22663

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVAAIGRIETVEPLAADAQAGVRLGIHAGGLPLADVALGDSIAIQGACMTVVAKND
CCCHHHHHHCCCCCCCCHHCCCCCCEEEEEEECCCCEEHEECCCCEEECCEEEEEEECCC
THFDVDVSRESLSKTVGLDRPGEVNLEKALRLADHIGGHLVSGHVDGLGTVSHFAPVGES
CEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCCCCC
YELRVRTSPELGRYFAYKGSVVVNGVSLTVNSVRDDATGCEFSINLIPHTLAVTTLKHLA
EEEEEECCCCCCCEEEEECCEEEEEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
VGGQVNLEIDLIARYVERMLTHTATGLPAAARPLAAV
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFTGIVAAIGRIETVEPLAADAQAGVRLGIHAGGLPLADVALGDSIAIQGACMTVVAKND
CCCHHHHHHCCCCCCCCHHCCCCCCEEEEEEECCCCEEHEECCCCEEECCEEEEEEECCC
THFDVDVSRESLSKTVGLDRPGEVNLEKALRLADHIGGHLVSGHVDGLGTVSHFAPVGES
CEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCCCCC
YELRVRTSPELGRYFAYKGSVVVNGVSLTVNSVRDDATGCEFSINLIPHTLAVTTLKHLA
EEEEEECCCCCCCEEEEECCEEEEEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
VGGQVNLEIDLIARYVERMLTHTATGLPAAARPLAAV
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]