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The map label for this gene is ribH [H]

Identifier: 17545431

GI number: 17545431

Start: 760036

End: 760530

Strand: Reverse

Name: ribH [H]

Synonym: RSc0712

Alternate gene names: 17545431

Gene position: 760530-760036 (Counterclockwise)

Preceding gene: 229259638

Following gene: 17545430

Centisome position: 20.46

GC content: 67.27

Gene sequence:

>495_bases
ATGGATCACGGCTTCTACCCGACCAACCTCGAAGGCGAAGGCCTGCGCATCGGCATCGTTCAGGCGCGTTTCAACGAGCC
CGTGTGCGAAGCGCTGCGCGAAGCGTGCGTCGCCGAGCTCGAGCAGTTGGGCGTGGCGGGCGAGGACGTGCTGCTGGTTA
CCGTGCCGGGCGCGCTGGAAGTGCCGCTCGCCCTGCAGAAGATGGCCGAATCGGGCCAGTTCGACGCGCTGATCGCGCTG
GGTGCCGTGATCCGCGGCGAGACCTATCATTTCGAGTTGGTGTCCAACGAGTCCGGCGCCGGCATCACGCGCGTCTGCCT
GGACTTCAACATCGCCATCGCCAACGGCATCCTGACCACCGACACCGACGCGCAGGCCCACGCCCGCACGCGCGAGAAGG
GCCGCGACTGCGCCCGCACCGCCATCGAAATGGCCAACCTGACCGCCGCCCTGGACGGCCTGCAAGACCACGGCCAGGAC
GACGAGAACGAATAA

Upstream 100 bases:

>100_bases
GCCGCCACGCATTGAGGTTCCGGCGGTGGCCGCGCGCCGCCGCTGCTGTCTTTCTTCTTCCCTGACGTTGCCCTGACGGC
GACACCAACCGGACTACACC

Downstream 100 bases:

>100_bases
GCGCGTTTGCGCAAAGGCATCACCATGACGCAAGACAATTCCCCGGCCAAGCCCAAGGCGCCGCCCAAGAGCGCGCGCCG
CCGTGCCCGCGAACTGGCGC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MDHGFYPTNLEGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALEVPLALQKMAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRVCLDFNIAIANGILTTDTDAQAHARTREKGRDCARTAIEMANLTAALDGLQDHGQD
DENE

Sequences:

>Translated_164_residues
MDHGFYPTNLEGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALEVPLALQKMAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRVCLDFNIAIANGILTTDTDAQAHARTREKGRDCARTAIEMANLTAALDGLQDHGQD
DENE
>Mature_164_residues
MDHGFYPTNLEGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALEVPLALQKMAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRVCLDFNIAIANGILTTDTDAQAHARTREKGRDCARTAIEMANLTAALDGLQDHGQD
DENE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=41.8439716312057, Blast_Score=102, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 17537; Mature: 17537

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHGFYPTNLEGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VPLALQKMAESGQFDALIALGAVIRGETYHFELVSNESGAGITRVCLDFNIAIANGILTT
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEEEEEEECCEEEC
DTDAQAHARTREKGRDCARTAIEMANLTAALDGLQDHGQDDENE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDHGFYPTNLEGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VPLALQKMAESGQFDALIALGAVIRGETYHFELVSNESGAGITRVCLDFNIAIANGILTT
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEEEEEEECCEEEC
DTDAQAHARTREKGRDCARTAIEMANLTAALDGLQDHGQDDENE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA