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The map label for this gene is trmB [H]

Identifier: 17545422

GI number: 17545422

Start: 750408

End: 751163

Strand: Reverse

Name: trmB [H]

Synonym: RSc0703

Alternate gene names: 17545422

Gene position: 751163-750408 (Counterclockwise)

Preceding gene: 17545423

Following gene: 17545417

Centisome position: 20.21

GC content: 70.11

Gene sequence:

>756_bases
ATGCAGCCCAACGAACAGCCGGGCACCGGCCCCGCCGACACCACGCTGGAACAGCAAGACACGGCCGCCGCCGAAGTCGG
CCATCCGCGCCGCATCCGCTCCTTCGTGCGCCGCGCGGGGCGGACCTCGACCGGCCAGCAGCGTGCCATCGATGAACTCG
GGCCACGCTTCCTGCTGCCCTATGCCGCCGCGCCGCTCGACTGGGAAGCGGCCTTCGGCCGCACCGGCGCCCGCCGCATC
TTCGAGATCGGCTTCGGCATGGGCGAGACTTCCGCGCACATCGCGCAACTGCGCCCCGATGACGACTTCCTCGGCGTGGA
GGTGCACGAACCGGGCGTGGGCGCGCTGCTCAAGCTGATCGGCGAGCGCGGCATCGGCAACGTCCGCATCGTCTCGCACG
ATGCGGTGGAGGTGCTGGCGCAGATGATTCCCGAAGGCACGCTGGATGGCATCCACGTGTTCTTTCCCGACCCGTGGCAC
AAGAAGCGCCACAACAAGCGGCGCCTGATCCAGGGGCCGTTCGTGGCCCGGCTGGCGGCACACCTCAGGCCGGGCGGCTA
TCTGCATTGCGCGACGGACTGGGAGGAATACGCCCACCAGATGCTGGAAGTGCTGTCCGCCGAGCCGCTGCTGGAGAATA
CCGCCGACGGCTTCGCCCCGCGCCCCGACTACCGCCCCGTCACCAAGTTCGAGAAGCGCGGCCTGCGCCTGGGCCACGGC
GTGTGGGACGTGGTGTTCCGCAAGCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
TTCGATTCCCATCGCCCGCTCCACCGAACAGTCTGAAGCCGCCCTCGGCGGCTTTTTTGTTGTCCGCCGCCGTCGCGCGG
CATGCGGCACGAACATCCTC

Downstream 100 bases:

>100_bases
CCCGACGGCCCCGCCTTATCGGGCGCCGAAATGCGTCTCGCCGAACAGGTTCTTGGCGTGGCGCGGAAACGCGCGCCAGT
ACTGCGGCGGCGCCTTGATC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MQPNEQPGTGPADTTLEQQDTAAAEVGHPRRIRSFVRRAGRTSTGQQRAIDELGPRFLLPYAAAPLDWEAAFGRTGARRI
FEIGFGMGETSAHIAQLRPDDDFLGVEVHEPGVGALLKLIGERGIGNVRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWH
KKRHNKRRLIQGPFVARLAAHLRPGGYLHCATDWEEYAHQMLEVLSAEPLLENTADGFAPRPDYRPVTKFEKRGLRLGHG
VWDVVFRKRAA

Sequences:

>Translated_251_residues
MQPNEQPGTGPADTTLEQQDTAAAEVGHPRRIRSFVRRAGRTSTGQQRAIDELGPRFLLPYAAAPLDWEAAFGRTGARRI
FEIGFGMGETSAHIAQLRPDDDFLGVEVHEPGVGALLKLIGERGIGNVRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWH
KKRHNKRRLIQGPFVARLAAHLRPGGYLHCATDWEEYAHQMLEVLSAEPLLENTADGFAPRPDYRPVTKFEKRGLRLGHG
VWDVVFRKRAA
>Mature_251_residues
MQPNEQPGTGPADTTLEQQDTAAAEVGHPRRIRSFVRRAGRTSTGQQRAIDELGPRFLLPYAAAPLDWEAAFGRTGARRI
FEIGFGMGETSAHIAQLRPDDDFLGVEVHEPGVGALLKLIGERGIGNVRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWH
KKRHNKRRLIQGPFVARLAAHLRPGGYLHCATDWEEYAHQMLEVLSAEPLLENTADGFAPRPDYRPVTKFEKRGLRLGHG
VWDVVFRKRAA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=51.9823788546256, Blast_Score=233, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27913; Mature: 27913

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPNEQPGTGPADTTLEQQDTAAAEVGHPRRIRSFVRRAGRTSTGQQRAIDELGPRFLLP
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCEEECH
YAAAPLDWEAAFGRTGARRIFEIGFGMGETSAHIAQLRPDDDFLGVEVHEPGVGALLKLI
HHCCCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHH
GERGIGNVRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWHKKRHNKRRLIQGPFVARLAA
HHCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCHHHHHCCHHHHHHHH
HLRPGGYLHCATDWEEYAHQMLEVLSAEPLLENTADGFAPRPDYRPVTKFEKRGLRLGHG
HCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCH
VWDVVFRKRAA
HHHHHHHHCCC
>Mature Secondary Structure
MQPNEQPGTGPADTTLEQQDTAAAEVGHPRRIRSFVRRAGRTSTGQQRAIDELGPRFLLP
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCEEECH
YAAAPLDWEAAFGRTGARRIFEIGFGMGETSAHIAQLRPDDDFLGVEVHEPGVGALLKLI
HHCCCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHH
GERGIGNVRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWHKKRHNKRRLIQGPFVARLAA
HHCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCHHHHHCCHHHHHHHH
HLRPGGYLHCATDWEEYAHQMLEVLSAEPLLENTADGFAPRPDYRPVTKFEKRGLRLGHG
HCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCH
VWDVVFRKRAA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA