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The map label for this gene is pilJ [H]

Identifier: 17545390

GI number: 17545390

Start: 714713

End: 716944

Strand: Direct

Name: pilJ [H]

Synonym: RSc0671

Alternate gene names: 17545390

Gene position: 714713-716944 (Clockwise)

Preceding gene: 17545389

Following gene: 17545391

Centisome position: 19.23

GC content: 65.05

Gene sequence:

>2232_bases
ATGGGGTTCAAGTGGTTCAACGCGGGTCGCCGTGCCGGGGGTGAGGCAGAGGCGCCGGAAGTTGCAGCGGAAGGCGCCGG
GGTCGCGGCCGTGCAGTTGGATAGCGCCCAGGTGGACGAGATCGATCCTGCTGGTCCGCCGTCGCAACTCAGCCACGGCC
CGATCGACAAGGTGGTCGGCTGGATCGAGCGGGTGCCGTTCGTGGCCCAGCAGCGCGTGTTCACGGTCGGCCTGCTGGCC
GGTCTGGTGGCGCTGCTGGCCTCGATCTACTTCGATAACCGGCAAGCCAACAACGGTTCGCTGCAGATCGAAATCGCCGG
TGACACGCTGATGCACTCGCAGCGTCTGGCCAAGGCAGTGCCGGTGGCGCTGCTGGGTAACGAGAGCGCCTTCACGCAGC
TCAAGGAAAGCCGTGCGCGGCTGCAGAGCAACCTCGAAGCGCTGAAGACCGGTAGCGCCGACCGCGGCTTGAGCGCCACG
GCCGGCGGTGAAGCCCAGGCGTTGCTCGACAAGTCCATCGAAGAGTGGAAGCGCAGCGAGAAGAGCGCCCAGGCGGTGCT
GGCCCAGGAAAAGACCCTGGTGTCGGTGGGTAAGACGCTGAACGTGTTCAATGCCTCCAACCCGGAACTGCTGGAAGAAG
CCGAGCAGATCTCGTCGATGAAGCTGCAGACCAACGCGCCGGCGCGTGAAGTGGCCGCGTCGTCGCAGCTGGTGATGCTG
ACGCAGCGTCTCGGTAAGAACATGAACGAGTTCCTGGCCGGTGAAGGCGTCAACCCGGAAACCGCGTTCCTGCTGGGCAA
GGACACCAACACCTTCCGCGAAACCCTGAACGGTCTGCTCAACGGCTCGGAAGCGCTGCGCCTGTCGGCAGCCACCGATC
CGGAAACCAAGGGCTATCTGCAAGAGCTGTCGACCCGTTTCGACAACGTGCAGAAGACCACGCAGATCATTCTGGAGAAC
CTGCCGGGCCTGATCGCCGCCAAGCGCGCCCAGCAGCAGATCTTCAACGACAACGAAAAGCTGCGTGCCGACCTGTCGGA
CCTGCAGTCGGCCTATTCGGGCAGCGTGCGCAGCCGTCCGCTGACGCTCGGCGCCGTCGTGCTGTCGGCCATCCTGACGG
TGGCCTGCCTGGCCGGTCTGGCGGCCCTGTACCTGCGCGACTCGCGTGTGCGTGCAATGGAAGCAGAAGCGCGCCAGCGT
GAAGCCGAAGAGCGCCGGATGGTGGAAAAGCGCAACAACGATGCCACCCAGGCCGCAATTCTGCGTCTGATGAACGAACT
GCAGGACATCGCAGACGGTGACTTGACCAAGCAGGCCACCGTGTCGGAAGACATTACCGGCGCGATCGCCGACTCGGTGA
ACTACACCGTGGAAGAACTGCGCGAGCTGGTCGGCCGGGTGCAGCAGACCGCCGAGCAGGTGACGCAGGCCTCGTCGGCG
GTGCAGACCACCTCCGACAAGCTGGTGCTCGCCTCCGAAGAACAGTCCCGCCAGATCCGCCAGACCGGCCAGTCGGTGGT
GGAGATGGCCGACCGCATTACGCAGGTCTCGCGCGGCGCCGCCGAATCCGCGAACGTGGCGCGCGCCTCGCTGGCTGCTG
CCGAGCAGGGTCAGGTGGCCGTGCAGAACGCGATCACGGGCATGAACGACATCCGCGACCAGATTCAGGACACCTCCAAG
CGGATCAAGCGCCTGGGCGAATCGTCGCAGGAGATCGGTGAAATCGTGGAGCTGATCTCGGACATTACCGAGCAGACCAA
CGTGCTGGCACTGAACGCAGCCATCCAGGCCGCCTCCGCAGGTGAAGCCGGCCGCGGCTTCTCCGTGGTGGCCGAGGAAG
TGCAGCGTCTGGCAGAACGTTCCGCCGAGGCAGCCAAGCAGATCGGTGCGCTGATCCGCACGATTCAGACCGACACCCAG
GACGCGGTGCACGCAATGGAGCGCAGCACGGCCGGTGTGGTGGAAGGGGCCAAGCTGTCGGACAACGCTGGTACCGCTCT
GGTGGAGATCGGCCGCGTGTCGCGCCAGCTTGCCGAACTGATTGAAAGCATCTCGCAAACGACTTCGCACGAAGCGACGC
TGGCTTCCGACGTGGCGCAGAACATCGAGCAGATCCTGCAGATGACGGAGCAGACGTCGACGGGGTCCCGTCAGACCGCG
CAATCGGTGCGCCAGCTCACGCAGCTTGCAGAAGAGCTGCGCGATTCGGTGGCCCGTTTCCGCATCGCCTGA

Upstream 100 bases:

>100_bases
TGGAAGGTGCGGACCTCGCTGATCACATGGGCGCGGCCGCGGATGGGAAATCCATGCCGTCACTCTCGACAAGGCGGCAT
CGACAAGACAAGAGGGTCCT

Downstream 100 bases:

>100_bases
CGGGCGTCGCCCCCGGATTTCGGGCGGGCGCGGCGCGCAAGCGCCGCACGCAACCGCCACACGCTTTTTGGAAGCCCTCA
TGCATCATCCATCTTCCGCC

Product: twitching motility transmembrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MGFKWFNAGRRAGGEAEAPEVAAEGAGVAAVQLDSAQVDEIDPAGPPSQLSHGPIDKVVGWIERVPFVAQQRVFTVGLLA
GLVALLASIYFDNRQANNGSLQIEIAGDTLMHSQRLAKAVPVALLGNESAFTQLKESRARLQSNLEALKTGSADRGLSAT
AGGEAQALLDKSIEEWKRSEKSAQAVLAQEKTLVSVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLVML
TQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDPETKGYLQELSTRFDNVQKTTQIILEN
LPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYSGSVRSRPLTLGAVVLSAILTVACLAGLAALYLRDSRVRAMEAEARQR
EAEERRMVEKRNNDATQAAILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQASSA
VQTTSDKLVLASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQVAVQNAITGMNDIRDQIQDTSK
RIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQ
DAVHAMERSTAGVVEGAKLSDNAGTALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILQMTEQTSTGSRQTA
QSVRQLTQLAEELRDSVARFRIA

Sequences:

>Translated_743_residues
MGFKWFNAGRRAGGEAEAPEVAAEGAGVAAVQLDSAQVDEIDPAGPPSQLSHGPIDKVVGWIERVPFVAQQRVFTVGLLA
GLVALLASIYFDNRQANNGSLQIEIAGDTLMHSQRLAKAVPVALLGNESAFTQLKESRARLQSNLEALKTGSADRGLSAT
AGGEAQALLDKSIEEWKRSEKSAQAVLAQEKTLVSVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLVML
TQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDPETKGYLQELSTRFDNVQKTTQIILEN
LPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYSGSVRSRPLTLGAVVLSAILTVACLAGLAALYLRDSRVRAMEAEARQR
EAEERRMVEKRNNDATQAAILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQASSA
VQTTSDKLVLASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQVAVQNAITGMNDIRDQIQDTSK
RIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQ
DAVHAMERSTAGVVEGAKLSDNAGTALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILQMTEQTSTGSRQTA
QSVRQLTQLAEELRDSVARFRIA
>Mature_742_residues
GFKWFNAGRRAGGEAEAPEVAAEGAGVAAVQLDSAQVDEIDPAGPPSQLSHGPIDKVVGWIERVPFVAQQRVFTVGLLAG
LVALLASIYFDNRQANNGSLQIEIAGDTLMHSQRLAKAVPVALLGNESAFTQLKESRARLQSNLEALKTGSADRGLSATA
GGEAQALLDKSIEEWKRSEKSAQAVLAQEKTLVSVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLVMLT
QRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDPETKGYLQELSTRFDNVQKTTQIILENL
PGLIAAKRAQQQIFNDNEKLRADLSDLQSAYSGSVRSRPLTLGAVVLSAILTVACLAGLAALYLRDSRVRAMEAEARQRE
AEERRMVEKRNNDATQAAILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQASSAV
QTTSDKLVLASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQVAVQNAITGMNDIRDQIQDTSKR
IKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQD
AVHAMERSTAGVVEGAKLSDNAGTALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILQMTEQTSTGSRQTAQ
SVRQLTQLAEELRDSVARFRIA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=326, Percent_Identity=30.0613496932515, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788194, Length=320, Percent_Identity=29.375, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788195, Length=325, Percent_Identity=27.6923076923077, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789453, Length=282, Percent_Identity=30.8510638297872, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787690, Length=283, Percent_Identity=31.8021201413428, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 79661; Mature: 79530

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKWFNAGRRAGGEAEAPEVAAEGAGVAAVQLDSAQVDEIDPAGPPSQLSHGPIDKVVG
CCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
WIERVPFVAQQRVFTVGLLAGLVALLASIYFDNRQANNGSLQIEIAGDTLMHSQRLAKAV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHC
PVALLGNESAFTQLKESRARLQSNLEALKTGSADRGLSATAGGEAQALLDKSIEEWKRSE
CHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KSAQAVLAQEKTLVSVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLVML
HHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHH
TQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDPETKGYL
HHHHCCCHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHEEEECCCCCCHHHHH
QELSTRFDNVQKTTQIILENLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYSGSVRSRP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
LTLGAVVLSAILTVACLAGLAALYLRDSRVRAMEAEARQREAEERRMVEKRNNDATQAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQASSA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQTTSDKLVLASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQVA
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
VQNAITGMNDIRDQIQDTSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASA
HHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCC
GEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQDAVHAMERSTAGVVEGAKLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
DNAGTALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILQMTEQTSTGSRQTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QSVRQLTQLAEELRDSVARFRIA
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GFKWFNAGRRAGGEAEAPEVAAEGAGVAAVQLDSAQVDEIDPAGPPSQLSHGPIDKVVG
CCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
WIERVPFVAQQRVFTVGLLAGLVALLASIYFDNRQANNGSLQIEIAGDTLMHSQRLAKAV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHC
PVALLGNESAFTQLKESRARLQSNLEALKTGSADRGLSATAGGEAQALLDKSIEEWKRSE
CHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KSAQAVLAQEKTLVSVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLVML
HHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHH
TQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDPETKGYL
HHHHCCCHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHEEEECCCCCCHHHHH
QELSTRFDNVQKTTQIILENLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYSGSVRSRP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
LTLGAVVLSAILTVACLAGLAALYLRDSRVRAMEAEARQREAEERRMVEKRNNDATQAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQASSA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQTTSDKLVLASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQVA
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
VQNAITGMNDIRDQIQDTSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASA
HHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCC
GEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQDAVHAMERSTAGVVEGAKLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
DNAGTALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILQMTEQTSTGSRQTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QSVRQLTQLAEELRDSVARFRIA
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]