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The map label for this gene is ubiB

Identifier: 17545180

GI number: 17545180

Start: 491857

End: 493434

Strand: Direct

Name: ubiB

Synonym: RSc0461

Alternate gene names: 17545180

Gene position: 491857-493434 (Clockwise)

Preceding gene: 17545179

Following gene: 17545181

Centisome position: 13.23

GC content: 67.24

Gene sequence:

>1578_bases
ATGACGCGCTTGTTCCGGCTGTGCAAGATCATCTTCGTCATCCTCTATCACGGGCTGGATCAGCTTGCGCTGTCGGGCTT
CAAGAGCCGGCGCATCCGCGCGCTGGTCTGGGTGCTGACCCTCGGCCGCAGGCAGACACGCCCGCGCGGTGAGCGCCTGC
GCCTGGCGCTGGAGCAGCTCGGCCCGATCTTCGTGAAGTTCGGGCAGGTCCTGTCCACGCGTCGCGACCTGCTGCCGCCC
GATGTGGCGGACGAACTGGCCAAGCTGCAGGACCGTGTGCCGCCGTTCGACCCCAAGATCGCGGCCGCCATTGTCGAGCG
ATCGCTGGGCAAGCCGCTGTCGGCGCTGTTCCATCGCTTCGACCATCATCCGGTGGCCAGCGCCTCGATCGCGCAGGTGC
ACTTCGCCACCCTGCGCGGCGGTCCCGACGACGGCCGCGAGGTGGCCGTGAAGGTGCTGCGTCCCGGCATGCTGCCGGTG
ATCGACAGCGATCTCGCGCTGATGCGCGACGTGGCCACGTGGATGGAGAAGCTGTGGGCGGACGGCAAGCGCCTGAAGCC
GCGCGAGGTGGTCGCCGAGTTCGACAAGTACCTGCACGACGAACTCGACCTGATGCGCGAGGCCGCCAACGCCAGCCAGC
TGCGCCGCAACTTCGCCAAGTCCGAGCTGCTGCTGGTGCCCGAGGTGTTCTGGGACTGGTGCACCTCCGAGGTGTTCGTG
ATGGAGCGCATGCACGGCGTGCGCGTCTCGCACGCCGACGAACTGCGTGCGGCCGGCGTCGACACGCATAAGCTGGCGCG
CGACGGCGTGGAGATCTTCTTCACGCAGGTGTTTCGCGACGGCTTCTTCCACGCCGACATGCACCCCGGCAACATCCTGG
TGAGCGTGGCGCCCGAGTCCCTGGGCCGCTATATCGCGCTGGATTTCGGCATCGTCGGGGCGCTGTCGGAATTCGACAAG
AATTACCTGGCGCAGAATTTCCTGGCCTTCTTCCAGCGCGATTACCACCGTGTGGCGCTGCTGCATGTCGAATCCGGCTG
GGCGCCCGAAGAGACCCGCGTGGAGGAGCTCGAAGGCGCCATTCGCGCCTGCTGCGAGCCGTACTTCGATCGCCCCCTCG
GCGAGATTTCGCTGGGGCTCGTGCTGATGCGGCTGTTCCAGACTTCGCGCCGCTTCAACGTGGAAGTGCAGCCGCAGCTG
GTGCTGCTGCAGAAGACGCTGCTCAACGTCGAGGGCCTGGGCCGCCAGCTCGATCCGGACCTGGATCTGTGGAAGACCGC
CAAGCCATTCCTGGAACGCTGGATGCACGAGCAGATCGGCTGGCGCGGCCTGGTTGATCGCCTCAAGATCGAGGCGCCGC
AATGGGCCAACATGCTGCCGGACTTCCCGCGCCTGGCGCACCAGATCCTGGAGCGCCACGCCCGCGATAACGGCAGCGCT
CAGACCGCCACGCTGAGCGCGCTGCTGGCCGAGCAGCGCCGCACCAACCGCCTGCTGTCGGCGGCGCTGCTGTTCATCGG
CGGCTTTGCGGTCGGCATCATTGCCACGCATGTGCTGGCGTGGCTGGCCCGCCACTGA

Upstream 100 bases:

>100_bases
TGGCGGCCGTGCGCGATGATGCGGCGCGCCTGGAGAAGCGGCTGGAGCGGCTCGAACGCGCCGCGCGCGGGACCTCGACG
GCACGGGCGGGCGAATCGGC

Downstream 100 bases:

>100_bases
CCGCCGACCGACTCTGACCCGCATGGCCCAACCGCCCGTCTTCACCACCCGCGATGCCGCCGCCCCCGCGTTCTGGGACG
AGCGCTTCAGCCGCGACCAC

Product: ubiquinone biosynthesis protein UbiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTRLFRLCKIIFVILYHGLDQLALSGFKSRRIRALVWVLTLGRRQTRPRGERLRLALEQLGPIFVKFGQVLSTRRDLLPP
DVADELAKLQDRVPPFDPKIAAAIVERSLGKPLSALFHRFDHHPVASASIAQVHFATLRGGPDDGREVAVKVLRPGMLPV
IDSDLALMRDVATWMEKLWADGKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSELLLVPEVFWDWCTSEVFV
MERMHGVRVSHADELRAAGVDTHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPESLGRYIALDFGIVGALSEFDK
NYLAQNFLAFFQRDYHRVALLHVESGWAPEETRVEELEGAIRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQL
VLLQKTLLNVEGLGRQLDPDLDLWKTAKPFLERWMHEQIGWRGLVDRLKIEAPQWANMLPDFPRLAHQILERHARDNGSA
QTATLSALLAEQRRTNRLLSAALLFIGGFAVGIIATHVLAWLARH

Sequences:

>Translated_525_residues
MTRLFRLCKIIFVILYHGLDQLALSGFKSRRIRALVWVLTLGRRQTRPRGERLRLALEQLGPIFVKFGQVLSTRRDLLPP
DVADELAKLQDRVPPFDPKIAAAIVERSLGKPLSALFHRFDHHPVASASIAQVHFATLRGGPDDGREVAVKVLRPGMLPV
IDSDLALMRDVATWMEKLWADGKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSELLLVPEVFWDWCTSEVFV
MERMHGVRVSHADELRAAGVDTHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPESLGRYIALDFGIVGALSEFDK
NYLAQNFLAFFQRDYHRVALLHVESGWAPEETRVEELEGAIRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQL
VLLQKTLLNVEGLGRQLDPDLDLWKTAKPFLERWMHEQIGWRGLVDRLKIEAPQWANMLPDFPRLAHQILERHARDNGSA
QTATLSALLAEQRRTNRLLSAALLFIGGFAVGIIATHVLAWLARH
>Mature_524_residues
TRLFRLCKIIFVILYHGLDQLALSGFKSRRIRALVWVLTLGRRQTRPRGERLRLALEQLGPIFVKFGQVLSTRRDLLPPD
VADELAKLQDRVPPFDPKIAAAIVERSLGKPLSALFHRFDHHPVASASIAQVHFATLRGGPDDGREVAVKVLRPGMLPVI
DSDLALMRDVATWMEKLWADGKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSELLLVPEVFWDWCTSEVFVM
ERMHGVRVSHADELRAAGVDTHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPESLGRYIALDFGIVGALSEFDKN
YLAQNFLAFFQRDYHRVALLHVESGWAPEETRVEELEGAIRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQLV
LLQKTLLNVEGLGRQLDPDLDLWKTAKPFLERWMHEQIGWRGLVDRLKIEAPQWANMLPDFPRLAHQILERHARDNGSAQ
TATLSALLAEQRRTNRLLSAALLFIGGFAVGIIATHVLAWLARH

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily

Homologues:

Organism=Homo sapiens, GI40254938, Length=261, Percent_Identity=32.183908045977, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI41393593, Length=274, Percent_Identity=31.021897810219, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI32261307, Length=316, Percent_Identity=30.379746835443, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI217035081, Length=209, Percent_Identity=33.4928229665072, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI34147522, Length=262, Percent_Identity=27.8625954198473, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI27363457, Length=269, Percent_Identity=27.8810408921933, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI217416386, Length=226, Percent_Identity=28.7610619469027, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI2367309, Length=472, Percent_Identity=54.2372881355932, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17559152, Length=278, Percent_Identity=27.3381294964029, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32565180, Length=200, Percent_Identity=32, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325148, Length=441, Percent_Identity=26.3038548752834, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323282, Length=260, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321319, Length=200, Percent_Identity=28, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI22024280, Length=255, Percent_Identity=30.1960784313725, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24662575, Length=262, Percent_Identity=29.3893129770992, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIB_RALSO (Q8Y275)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518582.1
- ProteinModelPortal:   Q8Y275
- GeneID:   1219265
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0461
- NMPDR:   fig|267608.1.peg.461
- HOGENOM:   HBG593509
- OMA:   LERFWAD
- ProtClustDB:   PRK04750
- BioCyc:   RSOL267608:RSC0461-MONOMER
- HAMAP:   MF_00414
- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR010232
- TIGRFAMs:   TIGR01982

Pfam domain/function: PF03109 ABC1; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 59804; Mature: 59673

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13e1d278)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TRLFRLCKIIFVILYHGLDQLALSGFKSRRIRALVWVLTLGRRQTRPRGERLRLALEQL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
GPIFVKFGQVLSTRRDLLPPDVADELAKLQDRVPPFDPKIAAAIVERSLGKPLSALFHRF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
DHHPVASASIAQVHFATLRGGPDDGREVAVKVLRPGMLPVIDSDLALMRDVATWMEKLWA
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHHC
DGKRLKPREVVAEFDKYLHDELDLMREAANASQLRRNFAKSELLLVPEVFWDWCTSEVFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
MERMHGVRVSHADELRAAGVDTHKLARDGVEIFFTQVFRDGFFHADMHPGNILVSVAPES
HHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHH
LGRYIALDFGIVGALSEFDKNYLAQNFLAFFQRDYHRVALLHVESGWAPEETRVEELEGA
HCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEECCCCCCCHHHHHHHHHH
IRACCEPYFDRPLGEISLGLVLMRLFQTSRRFNVEVQPQLVLLQKTLLNVEGLGRQLDPD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCCCCCC
LDLWKTAKPFLERWMHEQIGWRGLVDRLKIEAPQWANMLPDFPRLAHQILERHARDNGSA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCH
QTATLSALLAEQRRTNRLLSAALLFIGGFAVGIIATHVLAWLARH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852