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The map label for this gene is bah [H]

Identifier: 17545158

GI number: 17545158

Start: 466349

End: 467302

Strand: Direct

Name: bah [H]

Synonym: RSc0439

Alternate gene names: 17545158

Gene position: 466349-467302 (Clockwise)

Preceding gene: 17545155

Following gene: 17545159

Centisome position: 12.55

GC content: 70.55

Gene sequence:

>954_bases
ATGAGCTGGCAGAACTGGGTGGTGTCGTGGCTGGCGCGCAATCGCATCAAGAAGCCGACCGAGCGCTTGCCGTTCGACCC
GATCGCAACGCGCGTCAACGCAGAGACGCGATCGATGCCGATGCCGGTGCGCCTGCCGCCGGACTGGCGCATCGTTCGGG
CCGACGGCGCGCTGCCCGGTGAATGGATCGAGCCGGCCGATTCCGACAGTCTCGGCGATGCGCCGCCGGTGGTGTTCTAT
CTGCACGGCGGCGGCTACTTTTTCTGCAGCCCGCGCACGCATCGCTCCATCACCATCGGTCTGGCCGTGCATGCACGGGC
GCGCGTGTTCGCGCTGGATTACCGCCTGGCGCCCGAGCATCCGTTTCCCGCAGCCGTGCTCGATGCGCTGGCCGGCTATC
GGGGCTTGCTGGCGCAGCGGGTCGCGCCGTCGCGCATCGTGGTGGCGGGGGATTCGGCGGGCGGCGGGCTGTCGCTGGCG
CTGCTGGTGGCCCTGCGCGATGCCGGCGATCCGCTGCCGGCCGGCGCGATCCTGTATTCGCCGTGGACCGATCTGGCCGC
CACCGGCGCCAGCCTGGTCGAGAACGATGCCTCCGACGTGATGTTCCGCGGTGCCTGGATGGCGCGCGGCGCGACGCTCT
ATCTGGGCGACTCCGGCGTGCCGGCCGACCATCCGCTGGCCTCGCCGCTGTACGCCGATTTCACCGGGCTGCCGCCGCTG
CACTGCTATGTCAGCACCAGCGAAGTGCTGCGCGACGATACCCTCCGGATGGCCGAGCGGGCGCGCGCCGCGGGCGTATC
CGTTTCGGTGGAATTGGGCCGGCGGCTCCCACATGTGTGGCCGATCTTCTATCCTTTCTTACCAGAGGCGCGCAAGGCGC
TGCGGCAGTCCGGCGATGCGGTGCGTCGCATGACGGCGGGCCGCGCGGTGCCGGCGGAATGCGCGCCGGCATGA

Upstream 100 bases:

>100_bases
GACGCGACCGGTCGGCGGGCACATGGCCGGCGCGACGCGCGCCCATTGTCCGGATGGCGCAACCCCGCTACGCTGGACGT
TTCTTGGCACGGGGCAGGCA

Downstream 100 bases:

>100_bases
CACAGACACAGACGGCGCCCGAAAAGCGCTGCATGCACGAATGGAATGGCATCGAACGGGGAGCCCATCATGCGTCGGGT
GATGGCATTGGTGTTGGCGT

Product: lipase/esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSWQNWVVSWLARNRIKKPTERLPFDPIATRVNAETRSMPMPVRLPPDWRIVRADGALPGEWIEPADSDSLGDAPPVVFY
LHGGGYFFCSPRTHRSITIGLAVHARARVFALDYRLAPEHPFPAAVLDALAGYRGLLAQRVAPSRIVVAGDSAGGGLSLA
LLVALRDAGDPLPAGAILYSPWTDLAATGASLVENDASDVMFRGAWMARGATLYLGDSGVPADHPLASPLYADFTGLPPL
HCYVSTSEVLRDDTLRMAERARAAGVSVSVELGRRLPHVWPIFYPFLPEARKALRQSGDAVRRMTAGRAVPAECAPA

Sequences:

>Translated_317_residues
MSWQNWVVSWLARNRIKKPTERLPFDPIATRVNAETRSMPMPVRLPPDWRIVRADGALPGEWIEPADSDSLGDAPPVVFY
LHGGGYFFCSPRTHRSITIGLAVHARARVFALDYRLAPEHPFPAAVLDALAGYRGLLAQRVAPSRIVVAGDSAGGGLSLA
LLVALRDAGDPLPAGAILYSPWTDLAATGASLVENDASDVMFRGAWMARGATLYLGDSGVPADHPLASPLYADFTGLPPL
HCYVSTSEVLRDDTLRMAERARAAGVSVSVELGRRLPHVWPIFYPFLPEARKALRQSGDAVRRMTAGRAVPAECAPA
>Mature_316_residues
SWQNWVVSWLARNRIKKPTERLPFDPIATRVNAETRSMPMPVRLPPDWRIVRADGALPGEWIEPADSDSLGDAPPVVFYL
HGGGYFFCSPRTHRSITIGLAVHARARVFALDYRLAPEHPFPAAVLDALAGYRGLLAQRVAPSRIVVAGDSAGGGLSLAL
LVALRDAGDPLPAGAILYSPWTDLAATGASLVENDASDVMFRGAWMARGATLYLGDSGVPADHPLASPLYADFTGLPPLH
CYVSTSEVLRDDTLRMAERARAAGVSVSVELGRRLPHVWPIFYPFLPEARKALRQSGDAVRRMTAGRAVPAECAPA

Specific function: This protein removes the N-acetyl group from bialaphos as one of the final steps of the bialaphos biosynthetic pathway [H]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=238, Percent_Identity=28.1512605042017, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=24.5283018867925, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656084, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24656076, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20130169, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34249; Mature: 34117

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQNWVVSWLARNRIKKPTERLPFDPIATRVNAETRSMPMPVRLPPDWRIVRADGALPG
CCHHHHHHHHHHHHHCCCCHHHCCCCHHHHCCCCHHHCCCCCEECCCCCEEEEECCCCCC
EWIEPADSDSLGDAPPVVFYLHGGGYFFCSPRTHRSITIGLAVHARARVFALDYRLAPEH
CCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEEEECCEEEEEEEEEECCCC
PFPAAVLDALAGYRGLLAQRVAPSRIVVAGDSAGGGLSLALLVALRDAGDPLPAGAILYS
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEC
PWTDLAATGASLVENDASDVMFRGAWMARGATLYLGDSGVPADHPLASPLYADFTGLPPL
CCHHHHHCCHHHHHCCCHHHEEECHHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCE
HCYVSTSEVLRDDTLRMAERARAAGVSVSVELGRRLPHVWPIFYPFLPEARKALRQSGDA
EEEECHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHCCHHHHHHHHHCCHH
VRRMTAGRAVPAECAPA
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SWQNWVVSWLARNRIKKPTERLPFDPIATRVNAETRSMPMPVRLPPDWRIVRADGALPG
CHHHHHHHHHHHHHCCCCHHHCCCCHHHHCCCCHHHCCCCCEECCCCCEEEEECCCCCC
EWIEPADSDSLGDAPPVVFYLHGGGYFFCSPRTHRSITIGLAVHARARVFALDYRLAPEH
CCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEEEECCEEEEEEEEEECCCC
PFPAAVLDALAGYRGLLAQRVAPSRIVVAGDSAGGGLSLALLVALRDAGDPLPAGAILYS
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEC
PWTDLAATGASLVENDASDVMFRGAWMARGATLYLGDSGVPADHPLASPLYADFTGLPPL
CCHHHHHCCHHHHHCCCHHHEEECHHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCE
HCYVSTSEVLRDDTLRMAERARAAGVSVSVELGRRLPHVWPIFYPFLPEARKALRQSGDA
EEEECHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHCCHHHHHHHHHCCHH
VRRMTAGRAVPAECAPA
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2066341 [H]