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The map label for this gene is pfpI [H]

Identifier: 17545135

GI number: 17545135

Start: 443612

End: 444190

Strand: Reverse

Name: pfpI [H]

Synonym: RSc0416

Alternate gene names: 17545135

Gene position: 444190-443612 (Counterclockwise)

Preceding gene: 17545138

Following gene: 17545134

Centisome position: 11.95

GC content: 68.39

Gene sequence:

>579_bases
ATGGCGAAGAAGATTCTGATGCTGGTGGGCGACTATGTCGAAGACTACGAAGTCATGGTGCCCTTCCAGGCCCTGCAGAT
GGTCGGCCACACCGTGCACGCGGTCTGCCCGGACAAGCAGGCCGGCGAATCGATCGCCACCGCCGTGCACGACTTCGAGG
GCGCGCAGACGTATTCCGAGAAGCCGGGCCACCGCTTCACCGTCAATGCCAGCTTCGCCGACGTCGATCCCGCCGCCTAT
GACGCGCTGGTCGTGCCCGGCGGCCGCGCTCCCGAATACCTGCGCCTCGATCCGCGCGTGCTCGACATCGTGCGCCACTT
CAGCGGCGCCGGCAAACCCATCGCGGCGATCTGCCACGGCGCGCAGCTGCTGTCGGGGGCGGGCGTCCTGAAGGACCGCG
CGTGCTCCGCCTATCCGGCATGTGGCCCGGAAGTCACGGCAGCGGGCGGCACCTACCAGGACATCCCCGTCGACCAGGCC
CATACCGACGGCAACCTGGTGACCGCGCCCGCGTGGCCGGCCCACCCCGCATGGCTGGCGCAATTTCTTGAAGTCCTGGG
GACGCGGGTCCTACACTGA

Upstream 100 bases:

>100_bases
AGCCGGCTACCACCCGGCCCGGATGGCGCCGCACCACAATGCGGCAGGCACGGTGCACGGCAGCCGCCATGCACAAACTG
CAACCATACAGGAGACGACG

Downstream 100 bases:

>100_bases
TCGGACTGCCCGCGCGCCGGGCGCTCCCGCCCGGAGACCCCGCCATGTGCGAAATCTTCATCCGCGCCAATCCGCAGTCG
TACGAAACCCAGGCCCGCTC

Product: hypothetical protein

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKQAGESIATAVHDFEGAQTYSEKPGHRFTVNASFADVDPAAY
DALVVPGGRAPEYLRLDPRVLDIVRHFSGAGKPIAAICHGAQLLSGAGVLKDRACSAYPACGPEVTAAGGTYQDIPVDQA
HTDGNLVTAPAWPAHPAWLAQFLEVLGTRVLH

Sequences:

>Translated_192_residues
MAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKQAGESIATAVHDFEGAQTYSEKPGHRFTVNASFADVDPAAY
DALVVPGGRAPEYLRLDPRVLDIVRHFSGAGKPIAAICHGAQLLSGAGVLKDRACSAYPACGPEVTAAGGTYQDIPVDQA
HTDGNLVTAPAWPAHPAWLAQFLEVLGTRVLH
>Mature_191_residues
AKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKQAGESIATAVHDFEGAQTYSEKPGHRFTVNASFADVDPAAYD
ALVVPGGRAPEYLRLDPRVLDIVRHFSGAGKPIAAICHGAQLLSGAGVLKDRACSAYPACGPEVTAAGGTYQDIPVDQAH
TDGNLVTAPAWPAHPAWLAQFLEVLGTRVLH

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=189, Percent_Identity=34.9206349206349, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 20414; Mature: 20283

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKQAGESIATAVHDFEGAQTYSE
CCCHHEEHHHHHHHCCEEECCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCC
KPGHRFTVNASFADVDPAAYDALVVPGGRAPEYLRLDPRVLDIVRHFSGAGKPIAAICHG
CCCCEEEEECCCCCCCCHHCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHH
AQLLSGAGVLKDRACSAYPACGPEVTAAGGTYQDIPVDQAHTDGNLVTAPAWPAHPAWLA
HHHHHCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHH
QFLEVLGTRVLH
HHHHHHHHHHCC
>Mature Secondary Structure 
AKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKQAGESIATAVHDFEGAQTYSE
CCHHEEHHHHHHHCCEEECCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCC
KPGHRFTVNASFADVDPAAYDALVVPGGRAPEYLRLDPRVLDIVRHFSGAGKPIAAICHG
CCCCEEEEECCCCCCCCHHCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHH
AQLLSGAGVLKDRACSAYPACGPEVTAAGGTYQDIPVDQAHTDGNLVTAPAWPAHPAWLA
HHHHHCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHH
QFLEVLGTRVLH
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]