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The map label for this gene is mutM

Identifier: 17545118

GI number: 17545118

Start: 426720

End: 427586

Strand: Direct

Name: mutM

Synonym: RSc0399

Alternate gene names: 17545118

Gene position: 426720-427586 (Clockwise)

Preceding gene: 17545110

Following gene: 17545119

Centisome position: 11.48

GC content: 71.16

Gene sequence:

>867_bases
ATGCCTGAATTGCCCGAGGTCGAGGTCACCCGGCTGGGACTCGTCCCGCACCTGACAGGTCGGCGGATCGTTCGCGCCGT
GGTGCGGCATCACGGCCTGCGCTGGCCGGTCGATCCGGCGCTGCCCGAGCTGCTGGGCGGGCGCACGGTCGCGCGCGTGC
TGCGCCGGGGCAAGTATCTGCTGATCGAGTGCGTGCCCGACATTGCCCACGGGCCGCAGGCGGGGGCCGGCTGGCTGCTG
GTCCACCTGGGCATGACGGGCACCCTGCGCGTGCTGGAGACGCCTGCTGCACCCGGCACCCACGACCATCTCGACATCGA
GCTCGCGGATGCGGCCGGCCGGCCCATCACATTGCGCTACCGCGATCCGCGCCGCTTTGGTGCCGTGCTATGGCACGATG
GGGACGAGGCTGCGCTGTCGGCGCATCCGCTGCTGCGCAACCTCGGCATCGAGCCCTTCGACACGCGCTTCGACGGCGAC
TGGATGTACGCCCGCACCCGCGGCCGCAGCGCTGCCATCAAGACCGTGCTGCTGGCCGGCGACATCGTCGTCGGCGTCGG
CAATATCTATTGCTCGGAAAGCCTGTTCCGAGCCGGTATCCGGCCCACCACGGCGGCCGGGCGCATCAGCCGGCCGCGCT
ATGCGGCGCTGGCGGAGGCCATCCGCGCCACGCTGGCGGACGCCATTGCCCGCGGCGGCAGCACGCTGCGCGACTTCGTC
GGCTCGGACGGGCAGAGCGGCTATTTCCAGCTGGATGCGCTCGTCTACGACCGAGCAGGCTTGCCGTGCCGCGTGTGCGG
CACGCCGATCCGCCAGATCGTGCAGGGCCAGCGCTCGACCTTCTATTGCCCGGCCTGTCAGCGCTGA

Upstream 100 bases:

>100_bases
GTCAAAAGACTCCGGATTCGGTTCGGCTCATTGTAGCCTGACCGCTACAATTGCCGTCTGATCAATCACCGATTGGAGCC
GCGCATGCGGCGGAGGTTCG

Downstream 100 bases:

>100_bases
AGGCCGGCACGCCGTGAGGCAAACGGCGCCGTAGAGCGCTTTGCACAGCATTTCGCAAGATTTTCGAAGTGGGGTGTCGC
GTCGCCGATGGCATCCCGCA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPELPEVEVTRLGLVPHLTGRRIVRAVVRHHGLRWPVDPALPELLGGRTVARVLRRGKYLLIECVPDIAHGPQAGAGWLL
VHLGMTGTLRVLETPAAPGTHDHLDIELADAAGRPITLRYRDPRRFGAVLWHDGDEAALSAHPLLRNLGIEPFDTRFDGD
WMYARTRGRSAAIKTVLLAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALAEAIRATLADAIARGGSTLRDFV
GSDGQSGYFQLDALVYDRAGLPCRVCGTPIRQIVQGQRSTFYCPACQR

Sequences:

>Translated_288_residues
MPELPEVEVTRLGLVPHLTGRRIVRAVVRHHGLRWPVDPALPELLGGRTVARVLRRGKYLLIECVPDIAHGPQAGAGWLL
VHLGMTGTLRVLETPAAPGTHDHLDIELADAAGRPITLRYRDPRRFGAVLWHDGDEAALSAHPLLRNLGIEPFDTRFDGD
WMYARTRGRSAAIKTVLLAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALAEAIRATLADAIARGGSTLRDFV
GSDGQSGYFQLDALVYDRAGLPCRVCGTPIRQIVQGQRSTFYCPACQR
>Mature_287_residues
PELPEVEVTRLGLVPHLTGRRIVRAVVRHHGLRWPVDPALPELLGGRTVARVLRRGKYLLIECVPDIAHGPQAGAGWLLV
HLGMTGTLRVLETPAAPGTHDHLDIELADAAGRPITLRYRDPRRFGAVLWHDGDEAALSAHPLLRNLGIEPFDTRFDGDW
MYARTRGRSAAIKTVLLAGDIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALAEAIRATLADAIARGGSTLRDFVG
SDGQSGYFQLDALVYDRAGLPCRVCGTPIRQIVQGQRSTFYCPACQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=288, Percent_Identity=46.1805555555556, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_RALSO (Q8Y2D7)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518520.1
- ProteinModelPortal:   Q8Y2D7
- SMR:   Q8Y2D7
- GeneID:   1219203
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0399
- NMPDR:   fig|267608.1.peg.399
- HOGENOM:   HBG690070
- OMA:   FLKVHTK
- ProtClustDB:   PRK01103
- BioCyc:   RSOL267608:RSC0399-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31390; Mature: 31258

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 278-278 BINDING 101-101 BINDING 124-124 BINDING 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVTRLGLVPHLTGRRIVRAVVRHHGLRWPVDPALPELLGGRTVARVLRRGKYL
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCEE
LIECVPDIAHGPQAGAGWLLVHLGMTGTLRVLETPAAPGTHDHLDIELADAAGRPITLRY
EEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEEE
RDPRRFGAVLWHDGDEAALSAHPLLRNLGIEPFDTRFDGDWMYARTRGRSAAIKTVLLAG
CCCHHHCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHEEEC
DIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALAEAIRATLADAIARGGSTLRDFV
CEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GSDGQSGYFQLDALVYDRAGLPCRVCGTPIRQIVQGQRSTFYCPACQR
CCCCCCCEEEEEEEEECCCCCCEECCCHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure 
PELPEVEVTRLGLVPHLTGRRIVRAVVRHHGLRWPVDPALPELLGGRTVARVLRRGKYL
CCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCEE
LIECVPDIAHGPQAGAGWLLVHLGMTGTLRVLETPAAPGTHDHLDIELADAAGRPITLRY
EEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEEE
RDPRRFGAVLWHDGDEAALSAHPLLRNLGIEPFDTRFDGDWMYARTRGRSAAIKTVLLAG
CCCHHHCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHEEEC
DIVVGVGNIYCSESLFRAGIRPTTAAGRISRPRYAALAEAIRATLADAIARGGSTLRDFV
CEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GSDGQSGYFQLDALVYDRAGLPCRVCGTPIRQIVQGQRSTFYCPACQR
CCCCCCCEEEEEEEEECCCCCCEECCCHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852