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The map label for this gene is rpoH [H]

Identifier: 17545093

GI number: 17545093

Start: 401283

End: 402203

Strand: Reverse

Name: rpoH [H]

Synonym: RSc0374

Alternate gene names: 17545093

Gene position: 402203-401283 (Counterclockwise)

Preceding gene: 17545094

Following gene: 17545087

Centisome position: 10.82

GC content: 66.12

Gene sequence:

>921_bases
GTGAACGCAGTCATTCCCGTGAACACCGTTTCCGCCGTCGCCACGCAGCCGTCGTCCAATGCCTGGGCGCTAGCCTTTCC
CGGCAGCCTGGGCAACCTTGACGCCTATATCCAGAGCGTCAACCGCGTGCCGATGCTCACGGCCGAAGAAGAACAACAGC
TGGCCCGCGACTATCACGCGACCGGCAACCTCGACGCCGCGCGCCGGCTGGTGCTATCGCACCTGCGCCTGGTGGTGTCG
ATCGCCCGCCAGTACCTCGGCTATGGCCTGCCGCATGCCGACCTGATCCAGGAAGGCAACGTCGGCCTGATGAAGGCCGT
CAAGCGCTTCGACCCGGACCAGGGCGTGCGGCTGGTGTCGTACGCGATCCACTGGATCAAGGCCGAGATGCACGAGTACA
TCCTGAAGAACTGGCGCATGGTCAAGGTGGCGACCACCAAGGCCCAGCGCAAGCTGTTCTTCAACCTGCGCAGCCACAAG
CAGGACGCCCACGCCACCTTCACGTCCGACGAAGTCGATGCGGTGGCGCAAGAGCTGAACGTCAAGGGTGCCGAGGTGCG
CGAGATGGAAACGCGCCTGTCCGGCGGCGACATCGCGCTGGAGGGCCAGATCGACGACGGCGAAGAGTCGTTCGCCCCGA
TCGCCTACCTGGCCGATACGCACAACGAGCCCACCCGCGTGCTGGAAGGGCGCGGCCGCGACCGCTTGCAGAGCGAGGGC
ATCGAAACCGCCCTGGCCAAGCTGGACGACCGCAGCCGCCGCATCATCGAGGCGCGCTGGCTCCACGTCAACGACGACGG
CTCGGGCGGCGCCACGCTGCATGAACTGGCCGACGAGTTCGGCGTGTCGGCCGAGCGCATCCGCCAGATCGAATCGGCCG
CGATGAAGAAGATGAAGGGCGCGCTGCAGTCTTTTGCCTGA

Upstream 100 bases:

>100_bases
AGGAACTATCAGACAGCGCAAAAAGTCTGATTAGCACTCATCCAGCCGGAGTGCTAAGATGCCTCTCCAAACTTCCACTG
GATTTCGACAGGAGTCCTGA

Downstream 100 bases:

>100_bases
TCGACCGCCCACGCAACGAAAAGCCCGCCAACTGGCGGGCTTTTTCTTTGGGCGCGGGCTGGATCAGTCCAGCAGCAGCT
TGATGTCGTGCACGATCGGC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNAVIPVNTVSAVATQPSSNAWALAFPGSLGNLDAYIQSVNRVPMLTAEEEQQLARDYHATGNLDAARRLVLSHLRLVVS
IARQYLGYGLPHADLIQEGNVGLMKAVKRFDPDQGVRLVSYAIHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHK
QDAHATFTSDEVDAVAQELNVKGAEVREMETRLSGGDIALEGQIDDGEESFAPIAYLADTHNEPTRVLEGRGRDRLQSEG
IETALAKLDDRSRRIIEARWLHVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALQSFA

Sequences:

>Translated_306_residues
MNAVIPVNTVSAVATQPSSNAWALAFPGSLGNLDAYIQSVNRVPMLTAEEEQQLARDYHATGNLDAARRLVLSHLRLVVS
IARQYLGYGLPHADLIQEGNVGLMKAVKRFDPDQGVRLVSYAIHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHK
QDAHATFTSDEVDAVAQELNVKGAEVREMETRLSGGDIALEGQIDDGEESFAPIAYLADTHNEPTRVLEGRGRDRLQSEG
IETALAKLDDRSRRIIEARWLHVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALQSFA
>Mature_306_residues
MNAVIPVNTVSAVATQPSSNAWALAFPGSLGNLDAYIQSVNRVPMLTAEEEQQLARDYHATGNLDAARRLVLSHLRLVVS
IARQYLGYGLPHADLIQEGNVGLMKAVKRFDPDQGVRLVSYAIHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHK
QDAHATFTSDEVDAVAQELNVKGAEVREMETRLSGGDIALEGQIDDGEESFAPIAYLADTHNEPTRVLEGRGRDRLQSEG
IETALAKLDDRSRRIIEARWLHVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALQSFA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=277, Percent_Identity=53.7906137184115, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789098, Length=314, Percent_Identity=30.8917197452229, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=31.1203319502075, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33913; Mature: 33913

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVIPVNTVSAVATQPSSNAWALAFPGSLGNLDAYIQSVNRVPMLTAEEEQQLARDYHA
CCCEECCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHCC
TGNLDAARRLVLSHLRLVVSIARQYLGYGLPHADLIQEGNVGLMKAVKRFDPDQGVRLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHH
YAIHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHKQDAHATFTSDEVDAVAQELN
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHC
VKGAEVREMETRLSGGDIALEGQIDDGEESFAPIAYLADTHNEPTRVLEGRGRDRLQSEG
CCCCHHHHHHHHCCCCCEEEECCCCCCCHHCCCCCEEECCCCCCHHHHCCCCHHHHHHHH
IETALAKLDDRSRRIIEARWLHVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKG
HHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ALQSFA
HHHHCC
>Mature Secondary Structure
MNAVIPVNTVSAVATQPSSNAWALAFPGSLGNLDAYIQSVNRVPMLTAEEEQQLARDYHA
CCCEECCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHCC
TGNLDAARRLVLSHLRLVVSIARQYLGYGLPHADLIQEGNVGLMKAVKRFDPDQGVRLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHH
YAIHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHKQDAHATFTSDEVDAVAQELN
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHC
VKGAEVREMETRLSGGDIALEGQIDDGEESFAPIAYLADTHNEPTRVLEGRGRDRLQSEG
CCCCHHHHHHHHCCCCCEEEECCCCCCCHHCCCCCEEECCCCCCHHHHCCCCHHHHHHHH
IETALAKLDDRSRRIIEARWLHVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKG
HHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ALQSFA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168128; 10952301 [H]