Click here to switch to the map view.

The map label for this gene is dctB2 [H]

Identifier: 17545050

GI number: 17545050

Start: 362536

End: 364533

Strand: Direct

Name: dctB2 [H]

Synonym: RSc0331

Alternate gene names: 17545050

Gene position: 362536-364533 (Clockwise)

Preceding gene: 17545049

Following gene: 17545051

Centisome position: 9.75

GC content: 71.12

Gene sequence:

>1998_bases
ATGCGCCACGGCACCGAGCGGCTGATGGCCGGCGCGGCCCCGGAAGGCACGGCGAGCCCGTCCGCCGTTCACCACCCGCC
GATGTCCGAGCCCGATTTCCCGTTTTCCTCCGCCAGCCCGCGCCGCGGCTTCGGGTGGCTGGTGGCGGCCGCTCTGGTGG
CGGTGATGGCGCTGGTGTGCGCGACGACCTTCGTGGTGACCTGGAACCGCGGCCTGTCGCAGGCCCAGCAGGCCGCCGGC
GCCCGCGTCGACCGCTATGCCGCCAACCTCAAGAGCACGCTCGACCGCTACGAGTACCTGCCGGCGCTGGTCGCGCTGCA
TCCGTTCATCCACGACCTGCTGGCCCACCCGACGCCCGACAACGTCGCGCGCGCCAACCACTACCTGCGCGAGGTCAACG
ACCGCGCCCGCGCCACCGCCACCTACGTGATCGCCCCGACCGGCCTGGCCCTGGCGGCATCCAACTACAACCTGCCCGAT
AGCTTCGTCGGCGCCGAATACCTGTTCCGGCCGTATTTCCAGCAGGCGGCCGCCGGACACGTCGGGCGCTTCTACGGCAT
CGGCATCACGCGCGAGGAGCCCGGCTACTACATCTCGCAGCCGGTGATGCAGAACGGCAGCGTGATCGGCGTGACGGTGG
TCAAGCTCAACCTGGAGTGGTTCGGCCGCGCCAGCCATGATGCCTCCGAGCCGGTGATGGTGGCCGACGAGAACGGCGTG
ATCTTCCTGTCGTCGGTGCCGGCGTGGCAGTACCGCACGGTCGAGCCGCTCACGCCCGAGCTGCAGGCGCAGCTGCAGGC
GACCCGCCAGTACTTCCGCAAGCAGATCACGCCGCTGCCGCTGCAGCCCGAGATGCCGGCCTTCCTGAAGCTGGTCGGGC
GCATGCCCGACGGCGCGCGTCTGGTGCGCGTCGGCGACCGCATCAACGCGCCGCGCTACCTGCTGGTATCGCGCGAGCTG
GTCGAGCCCGACTGGCAGCTGATGTACCTGACGCCCATCGATCCGGTGATGGGCGCGGCGCGCAGCGCGACGGTCGCCGC
GGCCTTCGCCTTCGCCTTCGTCTGCCTGCTGCTGTTCTATTGGCGCCAGCGCCGGCTGCGCCTGAGGGAGATGCTGCGCG
GCCGCCGCCTGCTCGAAGCGGCCTACGACCAGCTGGAGCGCCGCGTCGAAGACCGCACCGCCGACCTGATGGCAACCAAC
GAGCAACTGCAGCACGAGATCGTCGACCGCACCCGCGCCGAAGCCGAGCTGCGCGCCACGCAGGACGAACTCGTGCAGGC
CAGCAAGCTTGCCGCCCTGGGGCAGATGGCGGCCGGCATCACGCACGAGCTCAACCAGCCGCTGGCTGCGCTGCGCACCT
TCTCCGACAACACGCGCATCCTGCTGGAGCGCGGCCAGCACGGCGCCGCCGCCGACAACCTGCGCGCCATCGCCGACCTG
ACCGAACGCATGGGCAAGATCACCGCGCAGCTCAAGCTGTTTGCCGGCCGCTCGCGCCGCAGGCTTGCCGATGTGCAGGT
GCGCGTGGCGCTCGACCACGTGCTGGCGCTGCTGCGCCCGCGCCTGGCCGAGGTGGAGACCGCGCTGCACTGGCGCCTGC
CCGAGGCCGCATGCGTGGTCCGCGCGGATGAGCTCAAGCTGGAGCAGGTGCTGATCAACCTGATCGGCAACGCGCTCGAC
GCCATCGCCGCCAACGATCTGCCGCGCCCCGGCCGCATCGACATCGACATCGGCCCGGCCGATGGCGCGCCCGCGCAGCT
GTGCATCGTCGTGCGCGACAATGGCCCCGGCATTGCGGCCGATGCCATGCCGCACCTGTTCGAACCGTTCTTCACCACCA
AGGAGATCGGCCAGGGCCTCGGCCTGGGGCTGGCGATTTCCACCAGCATCGCGCGCGACTTCGGCGGCTCGCTGTCGGCG
GCCAATGTGCCGGGCGGCGGCGCCCAGTTCACGCTGTCGCTGGTCCGCGCCGAGGTGGTGCCGGCCGCGACGCCCTGA

Upstream 100 bases:

>100_bases
GCCAGAGCCGGCGGTGCCCCACCGGGGCGTCGCCGGATGGCAAGCGTATGATGGCGGGGCCGCTCCTTCCGGACGGCCCC
GCTTTCTTTTGGAAAGTGTG

Downstream 100 bases:

>100_bases
CCCATTCCTTTCACCCAAACGATCCATGTCCGAAGGCTTGAACGTTCTCTTCATCGAAGACGATCCGCCCGTCCGCCAGG
CCACCGCGCAGAGCCTGGAG

Product: C4-dicarboxylate transport sensor kinase transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MRHGTERLMAGAAPEGTASPSAVHHPPMSEPDFPFSSASPRRGFGWLVAAALVAVMALVCATTFVVTWNRGLSQAQQAAG
ARVDRYAANLKSTLDRYEYLPALVALHPFIHDLLAHPTPDNVARANHYLREVNDRARATATYVIAPTGLALAASNYNLPD
SFVGAEYLFRPYFQQAAAGHVGRFYGIGITREEPGYYISQPVMQNGSVIGVTVVKLNLEWFGRASHDASEPVMVADENGV
IFLSSVPAWQYRTVEPLTPELQAQLQATRQYFRKQITPLPLQPEMPAFLKLVGRMPDGARLVRVGDRINAPRYLLVSREL
VEPDWQLMYLTPIDPVMGAARSATVAAAFAFAFVCLLLFYWRQRRLRLREMLRGRRLLEAAYDQLERRVEDRTADLMATN
EQLQHEIVDRTRAEAELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRILLERGQHGAAADNLRAIADL
TERMGKITAQLKLFAGRSRRRLADVQVRVALDHVLALLRPRLAEVETALHWRLPEAACVVRADELKLEQVLINLIGNALD
AIAANDLPRPGRIDIDIGPADGAPAQLCIVVRDNGPGIAADAMPHLFEPFFTTKEIGQGLGLGLAISTSIARDFGGSLSA
ANVPGGGAQFTLSLVRAEVVPAATP

Sequences:

>Translated_665_residues
MRHGTERLMAGAAPEGTASPSAVHHPPMSEPDFPFSSASPRRGFGWLVAAALVAVMALVCATTFVVTWNRGLSQAQQAAG
ARVDRYAANLKSTLDRYEYLPALVALHPFIHDLLAHPTPDNVARANHYLREVNDRARATATYVIAPTGLALAASNYNLPD
SFVGAEYLFRPYFQQAAAGHVGRFYGIGITREEPGYYISQPVMQNGSVIGVTVVKLNLEWFGRASHDASEPVMVADENGV
IFLSSVPAWQYRTVEPLTPELQAQLQATRQYFRKQITPLPLQPEMPAFLKLVGRMPDGARLVRVGDRINAPRYLLVSREL
VEPDWQLMYLTPIDPVMGAARSATVAAAFAFAFVCLLLFYWRQRRLRLREMLRGRRLLEAAYDQLERRVEDRTADLMATN
EQLQHEIVDRTRAEAELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRILLERGQHGAAADNLRAIADL
TERMGKITAQLKLFAGRSRRRLADVQVRVALDHVLALLRPRLAEVETALHWRLPEAACVVRADELKLEQVLINLIGNALD
AIAANDLPRPGRIDIDIGPADGAPAQLCIVVRDNGPGIAADAMPHLFEPFFTTKEIGQGLGLGLAISTSIARDFGGSLSA
ANVPGGGAQFTLSLVRAEVVPAATP
>Mature_665_residues
MRHGTERLMAGAAPEGTASPSAVHHPPMSEPDFPFSSASPRRGFGWLVAAALVAVMALVCATTFVVTWNRGLSQAQQAAG
ARVDRYAANLKSTLDRYEYLPALVALHPFIHDLLAHPTPDNVARANHYLREVNDRARATATYVIAPTGLALAASNYNLPD
SFVGAEYLFRPYFQQAAAGHVGRFYGIGITREEPGYYISQPVMQNGSVIGVTVVKLNLEWFGRASHDASEPVMVADENGV
IFLSSVPAWQYRTVEPLTPELQAQLQATRQYFRKQITPLPLQPEMPAFLKLVGRMPDGARLVRVGDRINAPRYLLVSREL
VEPDWQLMYLTPIDPVMGAARSATVAAAFAFAFVCLLLFYWRQRRLRLREMLRGRRLLEAAYDQLERRVEDRTADLMATN
EQLQHEIVDRTRAEAELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRILLERGQHGAAADNLRAIADL
TERMGKITAQLKLFAGRSRRRLADVQVRVALDHVLALLRPRLAEVETALHWRLPEAACVVRADELKLEQVLINLIGNALD
AIAANDLPRPGRIDIDIGPADGAPAQLCIVVRDNGPGIAADAMPHLFEPFFTTKEIGQGLGLGLAISTSIARDFGGSLSA
ANVPGGGAQFTLSLVRAEVVPAATP

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=32.7659574468085, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=30.3703703703704, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=26.6409266409266, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=286, Percent_Identity=27.972027972028, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=288, Percent_Identity=25.6944444444444, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=29.8449612403101, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=27.3809523809524, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72786; Mature: 72786

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHGTERLMAGAAPEGTASPSAVHHPPMSEPDFPFSSASPRRGFGWLVAAALVAVMALVC
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ATTFVVTWNRGLSQAQQAAGARVDRYAANLKSTLDRYEYLPALVALHPFIHDLLAHPTPD
HHHHHEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NVARANHYLREVNDRARATATYVIAPTGLALAASNYNLPDSFVGAEYLFRPYFQQAAAGH
HHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
VGRFYGIGITREEPGYYISQPVMQNGSVIGVTVVKLNLEWFGRASHDASEPVMVADENGV
CCCEEECCEECCCCCCEECCHHHCCCCEEEEEEEEEEHHHCCCCCCCCCCCEEEECCCCE
IFLSSVPAWQYRTVEPLTPELQAQLQATRQYFRKQITPLPLQPEMPAFLKLVGRMPDGAR
EEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCE
LVRVGDRINAPRYLLVSRELVEPDWQLMYLTPIDPVMGAARSATVAAAFAFAFVCLLLFY
EEEECCCCCCCEEEEEEHHHCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
WRQRRLRLREMLRGRRLLEAAYDQLERRVEDRTADLMATNEQLQHEIVDRTRAEAELRAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRILLERGQHGAAADNLRAIADL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
TERMGKITAQLKLFAGRSRRRLADVQVRVALDHVLALLRPRLAEVETALHWRLPEAACVV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEE
RADELKLEQVLINLIGNALDAIAANDLPRPGRIDIDIGPADGAPAQLCIVVRDNGPGIAA
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCH
DAMPHLFEPFFTTKEIGQGLGLGLAISTSIARDFGGSLSAANVPGGGAQFTLSLVRAEVV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
PAATP
CCCCC
>Mature Secondary Structure
MRHGTERLMAGAAPEGTASPSAVHHPPMSEPDFPFSSASPRRGFGWLVAAALVAVMALVC
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ATTFVVTWNRGLSQAQQAAGARVDRYAANLKSTLDRYEYLPALVALHPFIHDLLAHPTPD
HHHHHEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NVARANHYLREVNDRARATATYVIAPTGLALAASNYNLPDSFVGAEYLFRPYFQQAAAGH
HHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
VGRFYGIGITREEPGYYISQPVMQNGSVIGVTVVKLNLEWFGRASHDASEPVMVADENGV
CCCEEECCEECCCCCCEECCHHHCCCCEEEEEEEEEEHHHCCCCCCCCCCCEEEECCCCE
IFLSSVPAWQYRTVEPLTPELQAQLQATRQYFRKQITPLPLQPEMPAFLKLVGRMPDGAR
EEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCE
LVRVGDRINAPRYLLVSRELVEPDWQLMYLTPIDPVMGAARSATVAAAFAFAFVCLLLFY
EEEECCCCCCCEEEEEEHHHCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
WRQRRLRLREMLRGRRLLEAAYDQLERRVEDRTADLMATNEQLQHEIVDRTRAEAELRAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRILLERGQHGAAADNLRAIADL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
TERMGKITAQLKLFAGRSRRRLADVQVRVALDHVLALLRPRLAEVETALHWRLPEAACVV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEE
RADELKLEQVLINLIGNALDAIAANDLPRPGRIDIDIGPADGAPAQLCIVVRDNGPGIAA
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCH
DAMPHLFEPFFTTKEIGQGLGLGLAISTSIARDFGGSLSAANVPGGGAQFTLSLVRAEVV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
PAATP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]