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The map label for this gene is gcvA [H]

Identifier: 17545044

GI number: 17545044

Start: 357103

End: 358065

Strand: Direct

Name: gcvA [H]

Synonym: RSc0325

Alternate gene names: 17545044

Gene position: 357103-358065 (Clockwise)

Preceding gene: 17545039

Following gene: 17545049

Centisome position: 9.61

GC content: 69.78

Gene sequence:

>963_bases
ATGGCGGAAATCCGTGAGCCGATGCGGCTGCCGCCGCTGGGGGCGTTGCGCGTATTCGAGGCGGCCGCGCGTTACGAGAG
CTTCTCGCGCGCGGCCATTGAGCTGTTCGTCACGCACGGCGCGGTCAGCCACCAGATGCGCACACTGGAGGAGGAGCTGG
GCGTGCCGCTGTTCGAGCGGCGCGGCAAGCGCGTCACCCTGACCCACGCCGGGCGCACCTACGCCGAGCGCGTGCGCGAC
GCGCTCGACCAGATCGCCCAGGCCACGCACCAGCTGCGCGCGGGCAACCGCGACAATCGCCTGACCATCAGCACCATGCC
GTCGTTCGCGGCGCGCTGGCTGACCCCGCACATCGGCACCTTCATCGAGCGGCATCCGGAGCTGGAGGTGGAGCTGTTCT
CCAGCCCGGCGCTGGTCGACTTCGCGCGCGAGGAGGTGGACGTGGCGCTGCGCATGGGCTCGGGCAACTACCCCGGCCTG
TACGTGGAGACGCTGCTCGACGACGTGTTCTTCCCGATCTGCAGCCCGACGTTCAACGGCGGCCGGCTGCCGCGCACGCC
TGCCGAGATGGCCGGCATGACGCTGCTGCGCAGCGAGGGCGAGCAATGGAAGCCGTGGTTCGACGCGGCCGGCGTGGAGG
GCTTTCCCGAGCCGCGCGCCGGGCTGATGTTCCAGGACTCGTCGCTGCTGCTGCAAGCGGCGGCGGCCGGGCAGGGCATT
GCGCTGATCCGCCCGACGCTGGCGTTCAACGACCTGATGGCCGGCCGTGTCGTGCGCCTGTTCGACGTGACGATCCCCTG
CCCGTGGAACTACTACTTCGTCTGTCCGCACGGCGCGCTGCAGACGCCCAAGATGCAGGCTTTCCGCGCGTGGCTGCTGC
CGGAGATCGCCGCCTTCAAGCTCAAGCTGGACCGGCTGATGAGCGACACCACGGTGAGCCTAGGGCACGTCGCGAAGGGA
TAA

Upstream 100 bases:

>100_bases
GGAACCAGTCTAGGCGCTTGGTATCGGTGCACCAAACGATATATATTGATTTCTTTGATAAAAGAATTCACATAAAACGG
CACAAAATCCAGGAGGAAAC

Downstream 100 bases:

>100_bases
GCCGCCGCGGCTCGCGATAAAAAAACGCCCCGGTCATGCCGGGGCGTTTGCGTTGGCCGGGTGCCTTGCCGGCGAGCGGC
TACAGCACGACCTTGACCAT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MAEIREPMRLPPLGALRVFEAAARYESFSRAAIELFVTHGAVSHQMRTLEEELGVPLFERRGKRVTLTHAGRTYAERVRD
ALDQIAQATHQLRAGNRDNRLTISTMPSFAARWLTPHIGTFIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGL
YVETLLDDVFFPICSPTFNGGRLPRTPAEMAGMTLLRSEGEQWKPWFDAAGVEGFPEPRAGLMFQDSSLLLQAAAAGQGI
ALIRPTLAFNDLMAGRVVRLFDVTIPCPWNYYFVCPHGALQTPKMQAFRAWLLPEIAAFKLKLDRLMSDTTVSLGHVAKG

Sequences:

>Translated_320_residues
MAEIREPMRLPPLGALRVFEAAARYESFSRAAIELFVTHGAVSHQMRTLEEELGVPLFERRGKRVTLTHAGRTYAERVRD
ALDQIAQATHQLRAGNRDNRLTISTMPSFAARWLTPHIGTFIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGL
YVETLLDDVFFPICSPTFNGGRLPRTPAEMAGMTLLRSEGEQWKPWFDAAGVEGFPEPRAGLMFQDSSLLLQAAAAGQGI
ALIRPTLAFNDLMAGRVVRLFDVTIPCPWNYYFVCPHGALQTPKMQAFRAWLLPEIAAFKLKLDRLMSDTTVSLGHVAKG
>Mature_319_residues
AEIREPMRLPPLGALRVFEAAARYESFSRAAIELFVTHGAVSHQMRTLEEELGVPLFERRGKRVTLTHAGRTYAERVRDA
LDQIAQATHQLRAGNRDNRLTISTMPSFAARWLTPHIGTFIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGLY
VETLLDDVFFPICSPTFNGGRLPRTPAEMAGMTLLRSEGEQWKPWFDAAGVEGFPEPRAGLMFQDSSLLLQAAAAGQGIA
LIRPTLAFNDLMAGRVVRLFDVTIPCPWNYYFVCPHGALQTPKMQAFRAWLLPEIAAFKLKLDRLMSDTTVSLGHVAKG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=39.7260273972603, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=31.6326530612245, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=31.4189189189189, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=28.8659793814433, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786401, Length=283, Percent_Identity=27.5618374558304, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI157672245, Length=159, Percent_Identity=35.2201257861635, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787128, Length=142, Percent_Identity=34.5070422535211, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790262, Length=239, Percent_Identity=30.9623430962343, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=263, Percent_Identity=25.8555133079848, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=256, Percent_Identity=24.609375, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=256, Percent_Identity=27.734375, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081978, Length=171, Percent_Identity=30.4093567251462, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789204, Length=311, Percent_Identity=24.4372990353698, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35683; Mature: 35551

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIREPMRLPPLGALRVFEAAARYESFSRAAIELFVTHGAVSHQMRTLEEELGVPLFER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHH
RGKRVTLTHAGRTYAERVRDALDQIAQATHQLRAGNRDNRLTISTMPSFAARWLTPHIGT
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCHHHHH
FIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGLYVETLLDDVFFPICSPTFNG
HHHCCCCCEEEEECCCCHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
GRLPRTPAEMAGMTLLRSEGEQWKPWFDAAGVEGFPEPRAGLMFQDSSLLLQAAAAGQGI
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCE
ALIRPTLAFNDLMAGRVVRLFDVTIPCPWNYYFVCPHGALQTPKMQAFRAWLLPEIAAFK
EEEECHHHHHHHHHCCEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LKLDRLMSDTTVSLGHVAKG
HHHHHHHCCCCEECCCCCCC
>Mature Secondary Structure 
AEIREPMRLPPLGALRVFEAAARYESFSRAAIELFVTHGAVSHQMRTLEEELGVPLFER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHH
RGKRVTLTHAGRTYAERVRDALDQIAQATHQLRAGNRDNRLTISTMPSFAARWLTPHIGT
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCHHHHH
FIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGLYVETLLDDVFFPICSPTFNG
HHHCCCCCEEEEECCCCHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
GRLPRTPAEMAGMTLLRSEGEQWKPWFDAAGVEGFPEPRAGLMFQDSSLLLQAAAAGQGI
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCE
ALIRPTLAFNDLMAGRVVRLFDVTIPCPWNYYFVCPHGALQTPKMQAFRAWLLPEIAAFK
EEEECHHHHHHHHHCCEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LKLDRLMSDTTVSLGHVAKG
HHHHHHHCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]