Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 17545042

GI number: 17545042

Start: 355732

End: 356421

Strand: Reverse

Name: lipB [H]

Synonym: RSc0323

Alternate gene names: 17545042

Gene position: 356421-355732 (Counterclockwise)

Preceding gene: 17545043

Following gene: 17545041

Centisome position: 9.59

GC content: 68.26

Gene sequence:

>690_bases
ATGATTCCGATCGACATCGTCGAGCGCGGCCAGCAGGACTACACTGCCTGCTTCGACGCGATGCAGACCTTCACCGCCCA
GCGCGGTCCCGACACCCCCGACACGATCTGGCTGGTCGAGCACCCGCCGGTGTTCACGCTCGGACTGGCCGGCGATCCGG
CCCACCTGTTGGCGCCGGGCAACATTCCGCTGGTAAAGGTCGACCGTGGTGGACAGATCACCTATCACGGCCCCGGCCAA
GTGGTGGCTTACCTCCTGCTGGATCTGCGCCGCCGCGGGCTGTTCGTGAAGGCACTGGTCGACGCCATCGAGGCGGCCGT
CATCGATACGCTCGCCACGTATAATGTGGCCTCCGAACGGAAGGCCGGCGCGCCCGGCATCTACTTGTCGGCCGGGCCGC
ACCAGGGCGCCAAGATCGCCGCGCTGGGGCTGAAGATCCGCAACGGCTGCAGCTACCACGGCGTCAGCCTGAACCTGGCG
ATGGACCTGTCGCCCTTCACGCAGATCAACCCCTGCGGCTATGCCGGCCTGGAAACCGTCGACATGGTGACCGCCGGTGC
CCGCGACGCAAGCGGGCGCGCGGTCGATGCCGCCGACTGGCAGCGCGTGTCGCACGTGCTGGCCGACCGGCTGGTCGCCC
AATTGCAGCACCGCGCAAATGCGCACCCCGCCGAAGCCGCTACCGCGTAG

Upstream 100 bases:

>100_bases
GCGCCGGTATCGTCTTTTCCGCGCGGCAACGTTCGACCCTGCTGCCTTCCAGGGCAAAGGCGGCCCGCGCCGTTGCCGGC
CCTGCGCTACACTCGCGGGC

Downstream 100 bases:

>100_bases
CAGCCGGCAAGCAGGACCGAATGAATGCACGTGCCACAGGCCGTGCGGAGAACACGATGACCGATTCCGCCTCCGGCGCC
TCCGCAGTCGCCAACATCGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MIPIDIVERGQQDYTACFDAMQTFTAQRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPGNIPLVKVDRGGQITYHGPGQ
VVAYLLLDLRRRGLFVKALVDAIEAAVIDTLATYNVASERKAGAPGIYLSAGPHQGAKIAALGLKIRNGCSYHGVSLNLA
MDLSPFTQINPCGYAGLETVDMVTAGARDASGRAVDAADWQRVSHVLADRLVAQLQHRANAHPAEAATA

Sequences:

>Translated_229_residues
MIPIDIVERGQQDYTACFDAMQTFTAQRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPGNIPLVKVDRGGQITYHGPGQ
VVAYLLLDLRRRGLFVKALVDAIEAAVIDTLATYNVASERKAGAPGIYLSAGPHQGAKIAALGLKIRNGCSYHGVSLNLA
MDLSPFTQINPCGYAGLETVDMVTAGARDASGRAVDAADWQRVSHVLADRLVAQLQHRANAHPAEAATA
>Mature_229_residues
MIPIDIVERGQQDYTACFDAMQTFTAQRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPGNIPLVKVDRGGQITYHGPGQ
VVAYLLLDLRRRGLFVKALVDAIEAAVIDTLATYNVASERKAGAPGIYLSAGPHQGAKIAALGLKIRNGCSYHGVSLNLA
MDLSPFTQINPCGYAGLETVDMVTAGARDASGRAVDAADWQRVSHVLADRLVAQLQHRANAHPAEAATA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=175, Percent_Identity=52.5714285714286, Blast_Score=191, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323268, Length=130, Percent_Identity=33.0769230769231, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=184, Percent_Identity=30.4347826086957, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24296; Mature: 24296

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIDIVERGQQDYTACFDAMQTFTAQRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPG
CCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCEEECCC
NIPLVKVDRGGQITYHGPGQVVAYLLLDLRRRGLFVKALVDAIEAAVIDTLATYNVASER
CCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHC
KAGAPGIYLSAGPHQGAKIAALGLKIRNGCSYHGVSLNLAMDLSPFTQINPCGYAGLETV
CCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHH
DMVTAGARDASGRAVDAADWQRVSHVLADRLVAQLQHRANAHPAEAATA
HHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIPIDIVERGQQDYTACFDAMQTFTAQRGPDTPDTIWLVEHPPVFTLGLAGDPAHLLAPG
CCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCEEECCC
NIPLVKVDRGGQITYHGPGQVVAYLLLDLRRRGLFVKALVDAIEAAVIDTLATYNVASER
CCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHC
KAGAPGIYLSAGPHQGAKIAALGLKIRNGCSYHGVSLNLAMDLSPFTQINPCGYAGLETV
CCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHH
DMVTAGARDASGRAVDAADWQRVSHVLADRLVAQLQHRANAHPAEAATA
HHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA