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The map label for this gene is lipA [H]

Identifier: 17545041

GI number: 17545041

Start: 354674

End: 355675

Strand: Reverse

Name: lipA [H]

Synonym: RSc0322

Alternate gene names: 17545041

Gene position: 355675-354674 (Counterclockwise)

Preceding gene: 17545042

Following gene: 17545040

Centisome position: 9.57

GC content: 66.17

Gene sequence:

>1002_bases
ATGACCGATTCCGCCTCCGGCGCCTCCGCAGTCGCCAACATCGCCACCCCGTCGAACGAGCCGTACGACGCGACCCGCAA
GCAGAAGTCGCTCGACAAGACCGCGCGCATCCCCATCAAGATCGTGCCGGCCGAGAAGCTGAAGAAGCCGGAGTGGATCC
GCGTCAAGGCCGCCACCGGCAACTCACGCTTCTACGAGATCAAGGACATCCTGCGCGCCAACAACCTCGTGACGGTGTGC
GAGGAGGCGAGCTGCCCGAACATCGGCGAGTGCTTCGGCAAGGGCACGGCCACCTTCATGATCATGGGCGACAAGTGCAC
GCGCCGCTGCCCGTTCTGCGACGTCGGCCACGGCCGCCCCGACCCGCTCGATGCGAACGAGCCCGAGAACCTGGCCAAGA
CCATCGCCCAGCTCAGACTGAACTACGTGGTCATCACCAGCGTGGACCGCGACGACCTGCGCGACGGCGGTGCCCAGCAC
TATGTCGATTGCATCTCGCGCACGCGCGAGCTGTCGCCGGCCACGCGCATCGAGGTGCTGGTGCCGGACTTCCGCGGCCG
GCTGGAAAAGGCGCTGGACATCCTCCAGGCCTGCCCGCCGGACGTGATGAACCACAACATGGAAACCGTGCCGCGCCTGT
ACAAGCAGGCCCGCCCGGGCGCCGACTACGCGCACTCGCTGAAGCTGCTCAAGGACTTCAAGGCGCGCAACCCGAACCTC
CCGACCAAGTCCGGCCTGATGGTCGGCCTGGGCGAGACCGACGAGGAAATCCTGGAGGTGATGCGCGACATGCGCGAGCA
CGACATCGACATGCTGACCATCGGCCAGTATCTCGCTCCGTCGGGCCACCATCTGCCGGTGCTGCGCTACGTGCACCCGG
ACACCTTCAAGATGTTCGAGGAGAAAGCCTACGAGATGGGCTTCACGCACGCCGCTGTCGGCGCCATGGTGCGCAGCTCG
TACCACGCGGATCAGCAGGCGCACGAAGCCGGCTTCGCCTGA

Upstream 100 bases:

>100_bases
AGCACCGCGCAAATGCGCACCCCGCCGAAGCCGCTACCGCGTAGCAGCCGGCAAGCAGGACCGAATGAATGCACGTGCCA
CAGGCCGTGCGGAGAACACG

Downstream 100 bases:

>100_bases
TCGGCGCAGCGGTATCCGCCCCGGACAGCAAGGGCCTTGGGATCAAACCCAAGGCCCTTTTTCCTTGCCGGTGACCGGGA
CGGACGGACCGGCGCGGCCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTDSASGASAVANIATPSNEPYDATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATGNSRFYEIKDILRANNLVTVC
EEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDANEPENLAKTIAQLRLNYVVITSVDRDDLRDGGAQH
YVDCISRTRELSPATRIEVLVPDFRGRLEKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNL
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSS
YHADQQAHEAGFA

Sequences:

>Translated_333_residues
MTDSASGASAVANIATPSNEPYDATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATGNSRFYEIKDILRANNLVTVC
EEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDANEPENLAKTIAQLRLNYVVITSVDRDDLRDGGAQH
YVDCISRTRELSPATRIEVLVPDFRGRLEKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNL
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSS
YHADQQAHEAGFA
>Mature_332_residues
TDSASGASAVANIATPSNEPYDATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATGNSRFYEIKDILRANNLVTVCE
EASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDANEPENLAKTIAQLRLNYVVITSVDRDDLRDGGAQHY
VDCISRTRELSPATRIEVLVPDFRGRLEKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNLP
TKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSSY
HADQQAHEAGFA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=46.9387755102041, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI37577164, Length=256, Percent_Identity=47.65625, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI1786846, Length=304, Percent_Identity=64.8026315789474, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=255, Percent_Identity=43.921568627451, Blast_Score=217, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=45.360824742268, Blast_Score=253, Evalue=2e-68,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=46.875, Blast_Score=261, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 37085; Mature: 36954

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSASGASAVANIATPSNEPYDATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATG
CCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEECC
NSRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRP
CCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCC
DPLDANEPENLAKTIAQLRLNYVVITSVDRDDLRDGGAQHYVDCISRTRELSPATRIEVL
CCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEE
VPDFRGRLEKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNL
CCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFE
CCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEECCHHHHHHH
EKAYEMGFTHAAVGAMVRSSYHADQQAHEAGFA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDSASGASAVANIATPSNEPYDATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATG
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEECC
NSRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRP
CCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCC
DPLDANEPENLAKTIAQLRLNYVVITSVDRDDLRDGGAQHYVDCISRTRELSPATRIEVL
CCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEE
VPDFRGRLEKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNL
CCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PTKSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFE
CCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEECCHHHHHHH
EKAYEMGFTHAAVGAMVRSSYHADQQAHEAGFA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA