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The map label for this gene is bcr [H]

Identifier: 17544935

GI number: 17544935

Start: 238633

End: 239862

Strand: Direct

Name: bcr [H]

Synonym: RSc0216

Alternate gene names: 17544935

Gene position: 238633-239862 (Clockwise)

Preceding gene: 17544933

Following gene: 17544936

Centisome position: 6.42

GC content: 71.46

Gene sequence:

>1230_bases
ATGCAGCCCACCGCCTCGCCCCCGCAAGACCGCAGTCTCTGGATGCTGGCCGTCCTGAGCCTGCTGATGGCCTTCGCCTC
CATCTCCACCGACCTGTACCTGCCGGCGATGCCGGCCATGCAGGCCACCCTCGGTGCGAGCGCCGGTGCGCTCGAATGGA
CCATCTCGGGCTATCTGGTCGGCTTCAGCCTGGGGCAACTGCTGTGGGGCCCGATCGGTGACCGCACCGGCCGGCGGCTG
CCGATTGCGCTGGGCCTGCTGCTGTTCATCGGCGGCTCGGCCGGCTGTGCGCTGGCCGCCAGTGCCCCGGCCCTCATCGC
ATGGCGGGCGGTGCAGGCCGCGGGTGCTTGCGCCAGCGTAGTGCTGGCGCGCGCCATGGTGCGCGATCTGTACGAGGGCG
AGCGTGCCGCGCGCATGATGTCCACGCTGATGACGGTGATGGCGATCGCGCCGCTGGTGGGGCCGAGCGCCGGCGGCCTG
ATCCTGAAGCTGTGGTCGTGGCGCGCGATTTTCTGGACGCTGGTGATCGTGGGGTTGGCCACGCTGGCGGCGGTCGGCAC
GCTGCCCGAGACCCTGCCGGCCCACCGCCGCAACGATGCGCCGCTGCATCGCACGCTCGCGCGCTACGGCGTGCTGCTCC
GGCATCCGCGGTTGCTGGGCTACCTCGGCGTCGGCGGCTTCTTTTATGGCGGGATGTACGCCTATGTCGCCGGAACGCCG
TTCGCGTACATCGATTACTACCACGTCCCGGCCCAGCACTATGGGCTGCTGTTCGGGCTCGGGATCGTGGGCATCATGGC
CACCAACCTGCTCAACGCGAACCTCGTCGGGCGTTTCGGCGGCGACCGCCTGATGCGTGCCGGCGCTGTTGCCGCCGCGC
TGGCCGGCGCGGCTGTCGCGCTGGCTGCCCGCATGGGCTGGGGCGGGTTGATGGGCCTGGTGATTCCGCTTTTCGTGTTC
GTCTCCACCACCGGCTTCATCGTGGCCAACGCGATGACCGGTGCCCTGGGCACCTTCCCGCAGTTTGCCGGCTCGGTCTC
CGCGCTGGTCGGCGCCACGCAGTACGGCACCGGCATCCTCGGCTCGGCCCTGGTCGGCGCGCTGGCCAACGGCACGCCCG
TCCCCATGGGGCTGGTGATTGCGGCATGCGGGAGCGGAGGTGCGCTGGCCGCCGTCCTGCTCGTGCCGGCGCGCCGGCCG
ATGCCCGTGGCCCCAGCGGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
GATTATCAATTGTGGCTTGTCACTGGAGAAAGCCAGGCTGGGCTACTCAGTTGACAGCGCGGCGCATACAGTCCGAGCCA
TCCCGCGCTCCCTGGACGCC

Downstream 100 bases:

>100_bases
TCCCTCTCATCCCGTTTCACATGATCGGCTCGATATTCGACCAGCTGTGGGGTGCACGCATGAAGAAACGCCAACTCGGC
AGGAGCGGCCTGCAAGTCTC

Product: transport transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQPTASPPQDRSLWMLAVLSLLMAFASISTDLYLPAMPAMQATLGASAGALEWTISGYLVGFSLGQLLWGPIGDRTGRRL
PIALGLLLFIGGSAGCALAASAPALIAWRAVQAAGACASVVLARAMVRDLYEGERAARMMSTLMTVMAIAPLVGPSAGGL
ILKLWSWRAIFWTLVIVGLATLAAVGTLPETLPAHRRNDAPLHRTLARYGVLLRHPRLLGYLGVGGFFYGGMYAYVAGTP
FAYIDYYHVPAQHYGLLFGLGIVGIMATNLLNANLVGRFGGDRLMRAGAVAAALAGAAVALAARMGWGGLMGLVIPLFVF
VSTTGFIVANAMTGALGTFPQFAGSVSALVGATQYGTGILGSALVGALANGTPVPMGLVIAACGSGGALAAVLLVPARRP
MPVAPAAAG

Sequences:

>Translated_409_residues
MQPTASPPQDRSLWMLAVLSLLMAFASISTDLYLPAMPAMQATLGASAGALEWTISGYLVGFSLGQLLWGPIGDRTGRRL
PIALGLLLFIGGSAGCALAASAPALIAWRAVQAAGACASVVLARAMVRDLYEGERAARMMSTLMTVMAIAPLVGPSAGGL
ILKLWSWRAIFWTLVIVGLATLAAVGTLPETLPAHRRNDAPLHRTLARYGVLLRHPRLLGYLGVGGFFYGGMYAYVAGTP
FAYIDYYHVPAQHYGLLFGLGIVGIMATNLLNANLVGRFGGDRLMRAGAVAAALAGAAVALAARMGWGGLMGLVIPLFVF
VSTTGFIVANAMTGALGTFPQFAGSVSALVGATQYGTGILGSALVGALANGTPVPMGLVIAACGSGGALAAVLLVPARRP
MPVAPAAAG
>Mature_409_residues
MQPTASPPQDRSLWMLAVLSLLMAFASISTDLYLPAMPAMQATLGASAGALEWTISGYLVGFSLGQLLWGPIGDRTGRRL
PIALGLLLFIGGSAGCALAASAPALIAWRAVQAAGACASVVLARAMVRDLYEGERAARMMSTLMTVMAIAPLVGPSAGGL
ILKLWSWRAIFWTLVIVGLATLAAVGTLPETLPAHRRNDAPLHRTLARYGVLLRHPRLLGYLGVGGFFYGGMYAYVAGTP
FAYIDYYHVPAQHYGLLFGLGIVGIMATNLLNANLVGRFGGDRLMRAGAVAAALAGAAVALAARMGWGGLMGLVIPLFVF
VSTTGFIVANAMTGALGTFPQFAGSVSALVGATQYGTGILGSALVGALANGTPVPMGLVIAACGSGGALAAVLLVPARRP
MPVAPAAAG

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=394, Percent_Identity=35.0253807106599, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI48994889, Length=377, Percent_Identity=30.5039787798408, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082312, Length=340, Percent_Identity=30, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=36.144578313253, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790794, Length=226, Percent_Identity=26.1061946902655, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787065, Length=262, Percent_Identity=27.4809160305344, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=210, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=178, Percent_Identity=26.4044943820225, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319517, Length=203, Percent_Identity=26.6009852216749, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41864; Mature: 41864

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTASPPQDRSLWMLAVLSLLMAFASISTDLYLPAMPAMQATLGASAGALEWTISGYLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHH
GFSLGQLLWGPIGDRTGRRLPIALGLLLFIGGSAGCALAASAPALIAWRAVQAAGACASV
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHH
VLARAMVRDLYEGERAARMMSTLMTVMAIAPLVGPSAGGLILKLWSWRAIFWTLVIVGLA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TLAAVGTLPETLPAHRRNDAPLHRTLARYGVLLRHPRLLGYLGVGGFFYGGMYAYVAGTP
HHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
FAYIDYYHVPAQHYGLLFGLGIVGIMATNLLNANLVGRFGGDRLMRAGAVAAALAGAAVA
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHH
LAARMGWGGLMGLVIPLFVFVSTTGFIVANAMTGALGTFPQFAGSVSALVGATQYGTGIL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHH
GSALVGALANGTPVPMGLVIAACGSGGALAAVLLVPARRPMPVAPAAAG
HHHHHHHHCCCCCCCHHHHEEECCCCCCEEHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQPTASPPQDRSLWMLAVLSLLMAFASISTDLYLPAMPAMQATLGASAGALEWTISGYLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHH
GFSLGQLLWGPIGDRTGRRLPIALGLLLFIGGSAGCALAASAPALIAWRAVQAAGACASV
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHH
VLARAMVRDLYEGERAARMMSTLMTVMAIAPLVGPSAGGLILKLWSWRAIFWTLVIVGLA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TLAAVGTLPETLPAHRRNDAPLHRTLARYGVLLRHPRLLGYLGVGGFFYGGMYAYVAGTP
HHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
FAYIDYYHVPAQHYGLLFGLGIVGIMATNLLNANLVGRFGGDRLMRAGAVAAALAGAAVA
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHH
LAARMGWGGLMGLVIPLFVFVSTTGFIVANAMTGALGTFPQFAGSVSALVGATQYGTGIL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHH
GSALVGALANGTPVPMGLVIAACGSGGALAAVLLVPARRPMPVAPAAAG
HHHHHHHHCCCCCCCHHHHEEECCCCCCEEHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]