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The map label for this gene is yneJ [H]

Identifier: 17544933

GI number: 17544933

Start: 236487

End: 237353

Strand: Direct

Name: yneJ [H]

Synonym: RSc0214

Alternate gene names: 17544933

Gene position: 236487-237353 (Clockwise)

Preceding gene: 17544929

Following gene: 17544935

Centisome position: 6.36

GC content: 61.48

Gene sequence:

>867_bases
ATGAGAAAGCTTGATCTTGAATCCCTGCTGTTCTTCAAGTCGGTCGCAGATCTCGGGAGCATCACCCAGGCCGCCAGGCA
GCTGAACCGCGTGCAGTCGAACGTCACCACGCGCGTCAAGAATCTGGAAGAGCGCCTTGGCGTGAAGCTGTTTCTGAGGC
ACGGGAATCGGCTGACGCTCTCCGCAGACGGGGAACGGCTGCGGGGCTACGCCGAAAGGCTTCTCCGCCTCTCGGCAGAA
GCGGAATCCGCCATGCTGGTTCGTGCCCCGCAAGGGACATTGAAGCTGGGCACGCTGGAAAGCACCGCTGCCGCTCGATT
GCCGCCCGTGCTGTCTCAATTTCACCGGCGCTACCCTGACGTGCTCATCGAGCTGGTGACAGGCTCATCAGCGAAGCTGC
TCGACATGGTTCAGCGATACGAGGTTGACGCGGCGCTCATCTCTGAGCCGTTCCATGCTCCTGGGCTGGCAACGCAGGAG
GCGTTCGATGAGGTGTTGGTGCTGATTGCGCCACTGACCGCCACCCATGTCTTGAGCCACGATCATCTGCAGCGTTGCAC
CGTGATCGCCTTCTCGGCGGGCTGCTCGTACCGGCGCATCTTTGAGGAATGGCTGGCAAGCAACGGCATTGCGCCTGCCC
GGATCCTGGAGCTGGCGTCATATCACGCGATTGTGGCCTGCGTGGCCGCCGGAACGGGCATTGGCATCATGCCAAGATCC
GTGCTGAAGGCCGTGCATGCGGAGCCTCAACTACAGCTGCTCCCTCTGCCACCCAGGTATGCCGAGGTCAAAACGCATCT
CGTATGGCAGCCGGATCATGACTTTGCGGCGGTGGATGCGCTTCGACAGCACCTTCTCGAGTGCTGA

Upstream 100 bases:

>100_bases
TCTAACGGTCACGAGCGAGGGGGCAGTCAAACAAAGCTGTTCACTTTAGATGCGGCGTGATATTACGAAAAATGAATAGT
TGAGATGGTGGATTCACAAA

Downstream 100 bases:

>100_bases
GTCGGGCAGTCGCGAATCTTCAGCCAACTGCCGATACCCGAGCTGAGCCAGGACGCAATCACCCCGCCCCCAACTCCCCA
TACCAATAAGCCGCAGTCAC

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRKLDLESLLFFKSVADLGSITQAARQLNRVQSNVTTRVKNLEERLGVKLFLRHGNRLTLSADGERLRGYAERLLRLSAE
AESAMLVRAPQGTLKLGTLESTAAARLPPVLSQFHRRYPDVLIELVTGSSAKLLDMVQRYEVDAALISEPFHAPGLATQE
AFDEVLVLIAPLTATHVLSHDHLQRCTVIAFSAGCSYRRIFEEWLASNGIAPARILELASYHAIVACVAAGTGIGIMPRS
VLKAVHAEPQLQLLPLPPRYAEVKTHLVWQPDHDFAAVDALRQHLLEC

Sequences:

>Translated_288_residues
MRKLDLESLLFFKSVADLGSITQAARQLNRVQSNVTTRVKNLEERLGVKLFLRHGNRLTLSADGERLRGYAERLLRLSAE
AESAMLVRAPQGTLKLGTLESTAAARLPPVLSQFHRRYPDVLIELVTGSSAKLLDMVQRYEVDAALISEPFHAPGLATQE
AFDEVLVLIAPLTATHVLSHDHLQRCTVIAFSAGCSYRRIFEEWLASNGIAPARILELASYHAIVACVAAGTGIGIMPRS
VLKAVHAEPQLQLLPLPPRYAEVKTHLVWQPDHDFAAVDALRQHLLEC
>Mature_288_residues
MRKLDLESLLFFKSVADLGSITQAARQLNRVQSNVTTRVKNLEERLGVKLFLRHGNRLTLSADGERLRGYAERLLRLSAE
AESAMLVRAPQGTLKLGTLESTAAARLPPVLSQFHRRYPDVLIELVTGSSAKLLDMVQRYEVDAALISEPFHAPGLATQE
AFDEVLVLIAPLTATHVLSHDHLQRCTVIAFSAGCSYRRIFEEWLASNGIAPARILELASYHAIVACVAAGTGIGIMPRS
VLKAVHAEPQLQLLPLPPRYAEVKTHLVWQPDHDFAAVDALRQHLLEC

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=267, Percent_Identity=41.1985018726592, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI87082132, Length=242, Percent_Identity=29.3388429752066, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=253, Percent_Identity=26.8774703557312, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=28.9156626506024, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=28.4552845528455, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789204, Length=246, Percent_Identity=31.7073170731707, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788481, Length=252, Percent_Identity=30.5555555555556, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788626, Length=141, Percent_Identity=34.7517730496454, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788296, Length=226, Percent_Identity=26.5486725663717, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=25.609756097561, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786401, Length=166, Percent_Identity=32.5301204819277, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=24.8995983935743, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=25.2032520325203, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=239, Percent_Identity=25.1046025104602, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=196, Percent_Identity=31.1224489795918, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787601, Length=134, Percent_Identity=29.8507462686567, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=242, Percent_Identity=24.7933884297521, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=242, Percent_Identity=23.5537190082645, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787879, Length=304, Percent_Identity=23.3552631578947, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788706, Length=210, Percent_Identity=27.6190476190476, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31803; Mature: 31803

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLDLESLLFFKSVADLGSITQAARQLNRVQSNVTTRVKNLEERLGVKLFLRHGNRLTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEE
SADGERLRGYAERLLRLSAEAESAMLVRAPQGTLKLGTLESTAAARLPPVLSQFHRRYPD
ECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCHHHHHHCCHHHHHHHHHHHH
VLIELVTGSSAKLLDMVQRYEVDAALISEPFHAPGLATQEAFDEVLVLIAPLTATHVLSH
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DHLQRCTVIAFSAGCSYRRIFEEWLASNGIAPARILELASYHAIVACVAAGTGIGIMPRS
HHHHHHHHEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLKAVHAEPQLQLLPLPPRYAEVKTHLVWQPDHDFAAVDALRQHLLEC
HHHHHCCCCCEEEEECCCCHHHHHEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLDLESLLFFKSVADLGSITQAARQLNRVQSNVTTRVKNLEERLGVKLFLRHGNRLTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEE
SADGERLRGYAERLLRLSAEAESAMLVRAPQGTLKLGTLESTAAARLPPVLSQFHRRYPD
ECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCHHHHHHCCHHHHHHHHHHHH
VLIELVTGSSAKLLDMVQRYEVDAALISEPFHAPGLATQEAFDEVLVLIAPLTATHVLSH
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DHLQRCTVIAFSAGCSYRRIFEEWLASNGIAPARILELASYHAIVACVAAGTGIGIMPRS
HHHHHHHHEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLKAVHAEPQLQLLPLPPRYAEVKTHLVWQPDHDFAAVDALRQHLLEC
HHHHHCCCCCEEEEECCCCHHHHHEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]