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The map label for this gene is alkJ [H]

Identifier: 17544842

GI number: 17544842

Start: 145024

End: 146748

Strand: Reverse

Name: alkJ [H]

Synonym: RSc0123

Alternate gene names: 17544842

Gene position: 146748-145024 (Counterclockwise)

Preceding gene: 17544845

Following gene: 17544834

Centisome position: 3.95

GC content: 71.42

Gene sequence:

>1725_bases
ATGGCCACGCCCGCCGCCCCGCTCACCTTCGACTACGTCATCGTCGGCGCAGGCTCGGCCGGCTGCGCGCTGGCGAGCCG
CCTGACCGAAGACCCCGACGTGACGGTCGCGCTGCTGGAGGCCGGGCCGCACGATCATCATTTCTCCGTGTGGGTGCCGG
CGGGCTACGCGGCCTCGCTGCCGTTCCGGAGCCGGCGCAACTACGCCTACCTGACCACGCCGCAGCCGGGACTGGGCGGG
CGTCAGGGGTATCAGCCGCGCGGGCGCGGTCTGGGCGGCAGCTCATCGCTCAACGCGATGATCTACACCCGCGGCCACCG
GCACGACTACGACCACTGGGCCGCGCTCGGCTGCACCGGCTGGGGCTGGGACAGCGTGCTGCCCTACTTCAAGCGGTCGG
AATGCAACGCGCGTGTCGCGGGCCGCGACGATGATCCGCTGCACGGCGGCAACGGCCCGCTGCACGTGAGCGACCTGCGC
ACCGACAATCCGATCGCGCAGCGCTTCGTGGAAGCGGCGGTGGCGGCGGGCTACCGCCGCAACGATGATTTCAACGGCCC
CGAACAGGAAGGGGTCGGGCTCTACCAGGTCACGCAATACAACGGGGAGCGCTGGAACGCCGCGCGCGCCTACCTGCACG
GCGGTGACAGGGCCGACACCACGTTCAACCGCGGACGGCGCCGGCTGACGGTGCTGCCCGACACGCAGGCGCTGCGCATC
GTCTTCGAGGACAGGCGCGCGACGGGCGTGGTGGTGGACCGCGCCGGCCGCACCGAGACGCTGCACGCACGGCGCGAGGT
CATCGTGTCATCGGGGACGTTCGGTTCGCCGCAGTTGCTGATGGCCTCGGGCGTGGGTCCGGCCGCGCAGCTGCGCGCGC
TGGGGATTCCGGTGGTGCAGGACCTGCCCGGCGTCGGCCAGAACCTGCAGGACCACCTCGACGTGATCCTGCACAAGAAG
CTGTTCAACCTCGACCTGGTCGGCTATTCGCTGCGCGGCAGCGTGCGCATGCTGGGCGAGATCCTGCGCTACCGGCGCGA
GCGCCGCGGCATGATCGCCACCAACTTCGCCGAGGCGGGCGGCTTCATCAAGAGCCGGCCCGGCCTGCTTGAACCGGACC
TGCAACTGCATTTCGTGGTCGCCATGGCCGACAACCACAACCGCACGTTCCACTACGGCCACGGCTATTCGTGCCACGCA
TGCGTGCTGCGCCCGGAAAGCCGCGGCGAGGTGCGCCTGGCATCGGCCGATACGCGGCAGGCGCCGTGCATCGATCCGCG
CTTCCTGTCCGATCCGACCGACATGGCGGGCATGATGGCGGGCTTGCGCGCCGTGCGCAGCATCTTCGCGCAGCAGCCGC
TGGCCGAACTGGGCGGGCGCGAGCTGTATTCAGAAGGGATTCGCGGCGACGGCTCCGACGACGAAGCCGTGCGCGCGCTG
ATCCGCCGGCATGCCGACACCATCTACCACCCGGTCGGCACCTGCCGCATGGGCGGCGCGGGCGACCCCGCGGCGGTGGT
CGACCCCGAGCTGCGCGTGCGCGGCATCGAGGGCCTGCGGGTGATCGACGGCTCGGTGATGCCGACGCTCATCGGCGGCA
ACACCAACGCGCCCGTCATCATGATCGCCGAGCGCGCGGCCGACCTGATCCGCCGGAGCGGCCGGCCGGTGCTCAACGTG
GTGACGCAGGCCCCGGCACCGCCCGTGCCGGCCGAGGGGCACTAG

Upstream 100 bases:

>100_bases
TGTTTGAAACCCCAGGCGCGGCCTACGATGGAGCCATCAAAAATGATGGCGCGCCCGCGAGCGTCGCCCGCCTCGCAGCA
GCGTCCATCCGGAGCCCCCC

Downstream 100 bases:

>100_bases
GCGGGGGGGCGCGGCGCGTCAGTTGCCGGCGCTGTCGGTGGGGATGCTGAAGGAGTCGCGCAGCAGATGGTTCATCGGAA
TCTGCAGGTTCAGGTTGTTG

Product: alcohol dehydrogenase oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MATPAAPLTFDYVIVGAGSAGCALASRLTEDPDVTVALLEAGPHDHHFSVWVPAGYAASLPFRSRRNYAYLTTPQPGLGG
RQGYQPRGRGLGGSSSLNAMIYTRGHRHDYDHWAALGCTGWGWDSVLPYFKRSECNARVAGRDDDPLHGGNGPLHVSDLR
TDNPIAQRFVEAAVAAGYRRNDDFNGPEQEGVGLYQVTQYNGERWNAARAYLHGGDRADTTFNRGRRRLTVLPDTQALRI
VFEDRRATGVVVDRAGRTETLHARREVIVSSGTFGSPQLLMASGVGPAAQLRALGIPVVQDLPGVGQNLQDHLDVILHKK
LFNLDLVGYSLRGSVRMLGEILRYRRERRGMIATNFAEAGGFIKSRPGLLEPDLQLHFVVAMADNHNRTFHYGHGYSCHA
CVLRPESRGEVRLASADTRQAPCIDPRFLSDPTDMAGMMAGLRAVRSIFAQQPLAELGGRELYSEGIRGDGSDDEAVRAL
IRRHADTIYHPVGTCRMGGAGDPAAVVDPELRVRGIEGLRVIDGSVMPTLIGGNTNAPVIMIAERAADLIRRSGRPVLNV
VTQAPAPPVPAEGH

Sequences:

>Translated_574_residues
MATPAAPLTFDYVIVGAGSAGCALASRLTEDPDVTVALLEAGPHDHHFSVWVPAGYAASLPFRSRRNYAYLTTPQPGLGG
RQGYQPRGRGLGGSSSLNAMIYTRGHRHDYDHWAALGCTGWGWDSVLPYFKRSECNARVAGRDDDPLHGGNGPLHVSDLR
TDNPIAQRFVEAAVAAGYRRNDDFNGPEQEGVGLYQVTQYNGERWNAARAYLHGGDRADTTFNRGRRRLTVLPDTQALRI
VFEDRRATGVVVDRAGRTETLHARREVIVSSGTFGSPQLLMASGVGPAAQLRALGIPVVQDLPGVGQNLQDHLDVILHKK
LFNLDLVGYSLRGSVRMLGEILRYRRERRGMIATNFAEAGGFIKSRPGLLEPDLQLHFVVAMADNHNRTFHYGHGYSCHA
CVLRPESRGEVRLASADTRQAPCIDPRFLSDPTDMAGMMAGLRAVRSIFAQQPLAELGGRELYSEGIRGDGSDDEAVRAL
IRRHADTIYHPVGTCRMGGAGDPAAVVDPELRVRGIEGLRVIDGSVMPTLIGGNTNAPVIMIAERAADLIRRSGRPVLNV
VTQAPAPPVPAEGH
>Mature_573_residues
ATPAAPLTFDYVIVGAGSAGCALASRLTEDPDVTVALLEAGPHDHHFSVWVPAGYAASLPFRSRRNYAYLTTPQPGLGGR
QGYQPRGRGLGGSSSLNAMIYTRGHRHDYDHWAALGCTGWGWDSVLPYFKRSECNARVAGRDDDPLHGGNGPLHVSDLRT
DNPIAQRFVEAAVAAGYRRNDDFNGPEQEGVGLYQVTQYNGERWNAARAYLHGGDRADTTFNRGRRRLTVLPDTQALRIV
FEDRRATGVVVDRAGRTETLHARREVIVSSGTFGSPQLLMASGVGPAAQLRALGIPVVQDLPGVGQNLQDHLDVILHKKL
FNLDLVGYSLRGSVRMLGEILRYRRERRGMIATNFAEAGGFIKSRPGLLEPDLQLHFVVAMADNHNRTFHYGHGYSCHAC
VLRPESRGEVRLASADTRQAPCIDPRFLSDPTDMAGMMAGLRAVRSIFAQQPLAELGGRELYSEGIRGDGSDDEAVRALI
RRHADTIYHPVGTCRMGGAGDPAAVVDPELRVRGIEGLRVIDGSVMPTLIGGNTNAPVIMIAERAADLIRRSGRPVLNVV
TQAPAPPVPAEGH

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=556, Percent_Identity=42.9856115107914, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI1786503, Length=559, Percent_Identity=39.1771019677996, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17532301, Length=565, Percent_Identity=38.5840707964602, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24642055, Length=589, Percent_Identity=36.8421052631579, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI45551458, Length=576, Percent_Identity=36.2847222222222, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI45549471, Length=576, Percent_Identity=36.2847222222222, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=37.7391304347826, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24642048, Length=614, Percent_Identity=34.0390879478827, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI18859995, Length=576, Percent_Identity=36.2847222222222, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24642039, Length=578, Percent_Identity=36.159169550173, Blast_Score=296, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24642035, Length=581, Percent_Identity=33.9070567986231, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24650267, Length=583, Percent_Identity=35.1629502572899, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24642059, Length=580, Percent_Identity=34.6551724137931, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24642037, Length=582, Percent_Identity=35.0515463917526, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI17137792, Length=565, Percent_Identity=34.1592920353982, Blast_Score=272, Evalue=6e-73,
Organism=Drosophila melanogaster, GI18859993, Length=581, Percent_Identity=33.3907056798623, Blast_Score=264, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642051, Length=576, Percent_Identity=32.1180555555556, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24645930, Length=589, Percent_Identity=27.6740237691002, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24642057, Length=597, Percent_Identity=28.9782244556114, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 62254; Mature: 62123

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPAAPLTFDYVIVGAGSAGCALASRLTEDPDVTVALLEAGPHDHHFSVWVPAGYAASL
CCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCEECC
PFRSRRNYAYLTTPQPGLGGRQGYQPRGRGLGGSSSLNAMIYTRGHRHDYDHWAALGCTG
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEECCCC
WGWDSVLPYFKRSECNARVAGRDDDPLHGGNGPLHVSDLRTDNPIAQRFVEAAVAAGYRR
CCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
NDDFNGPEQEGVGLYQVTQYNGERWNAARAYLHGGDRADTTFNRGRRRLTVLPDTQALRI
CCCCCCCHHHCCEEEEEEECCCCCCCCEEHEEECCCCCCCHHHCCCEEEEECCCCCEEEE
VFEDRRATGVVVDRAGRTETLHARREVIVSSGTFGSPQLLMASGVGPAAQLRALGIPVVQ
EEECCCCCEEEEECCCCCHHHHHHHHEEEECCCCCCCCEEEECCCCHHHHHHHCCCCHHH
DLPGVGQNLQDHLDVILHKKLFNLDLVGYSLRGSVRMLGEILRYRRERRGMIATNFAEAG
CCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECHHHHC
GFIKSRPGLLEPDLQLHFVVAMADNHNRTFHYGHGYSCHACVLRPESRGEVRLASADTRQ
CCCCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCCCEEEEEECCCCCCCEEEECCCCCC
APCIDPRFLSDPTDMAGMMAGLRAVRSIFAQQPLAELGGRELYSEGIRGDGSDDEAVRAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCCCCHHHHHHH
IRRHADTIYHPVGTCRMGGAGDPAAVVDPELRVRGIEGLRVIDGSVMPTLIGGNTNAPVI
HHHHHHHHHCCCCCEECCCCCCCCEEECCCCEEECCCCEEEECCCCCEEEECCCCCCCEE
MIAERAADLIRRSGRPVLNVVTQAPAPPVPAEGH
EEHHHHHHHHHHCCCCEEEHEECCCCCCCCCCCC
>Mature Secondary Structure 
ATPAAPLTFDYVIVGAGSAGCALASRLTEDPDVTVALLEAGPHDHHFSVWVPAGYAASL
CCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCEECC
PFRSRRNYAYLTTPQPGLGGRQGYQPRGRGLGGSSSLNAMIYTRGHRHDYDHWAALGCTG
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEECCCC
WGWDSVLPYFKRSECNARVAGRDDDPLHGGNGPLHVSDLRTDNPIAQRFVEAAVAAGYRR
CCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
NDDFNGPEQEGVGLYQVTQYNGERWNAARAYLHGGDRADTTFNRGRRRLTVLPDTQALRI
CCCCCCCHHHCCEEEEEEECCCCCCCCEEHEEECCCCCCCHHHCCCEEEEECCCCCEEEE
VFEDRRATGVVVDRAGRTETLHARREVIVSSGTFGSPQLLMASGVGPAAQLRALGIPVVQ
EEECCCCCEEEEECCCCCHHHHHHHHEEEECCCCCCCCEEEECCCCHHHHHHHCCCCHHH
DLPGVGQNLQDHLDVILHKKLFNLDLVGYSLRGSVRMLGEILRYRRERRGMIATNFAEAG
CCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECHHHHC
GFIKSRPGLLEPDLQLHFVVAMADNHNRTFHYGHGYSCHACVLRPESRGEVRLASADTRQ
CCCCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCCCEEEEEECCCCCCCEEEECCCCCC
APCIDPRFLSDPTDMAGMMAGLRAVRSIFAQQPLAELGGRELYSEGIRGDGSDDEAVRAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCCCCHHHHHHH
IRRHADTIYHPVGTCRMGGAGDPAAVVDPELRVRGIEGLRVIDGSVMPTLIGGNTNAPVI
HHHHHHHHHCCCCCEECCCCCCCCEEECCCCEEECCCCEEEECCCCCEEEECCCCCCCEE
MIAERAADLIRRSGRPVLNVVTQAPAPPVPAEGH
EEHHHHHHHHHHCCCCEEEHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]