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The map label for this gene is thiC

Identifier: 17544832

GI number: 17544832

Start: 130368

End: 132263

Strand: Reverse

Name: thiC

Synonym: RSc0113

Alternate gene names: 17544832

Gene position: 132263-130368 (Counterclockwise)

Preceding gene: 17544834

Following gene: 17544831

Centisome position: 3.56

GC content: 65.88

Gene sequence:

>1896_bases
ATGCCCCAAGCCAAAAACGCCCCCGCCGCCTCATTCGACTCGCTGCAGACCGAGCTCGACCAGAAATTCGCCTACCCGGC
CTCCAGCAAGACCTACATCGCCGGCAGCCGCCCAGACCTGCGCGTGCCGATGCGCACCATCAAGCAGACCGCCACGCGCA
CCGACCAGGGCGAAATGCTGAACCCGCCGATTCCCGTGTACGACACCTCGGGCCCGTACTCCGACCCCGACGTGCACATC
GACCTGAAGGCGGGCCTTGCCCCGCTGCGCGCCAAGTGGATCGACGAGCGCGGCGACACCGTGGTGCTGCCGAGCCTGTC
GTCGGAATACGGCCAGGCACGCGCGCATGACCCGGCCACGGCCCACCTGCGCTTTGCGCAACTGACCAACCCGCGCCGCG
CCAAGCCGGGCGCGAACGTGAGCCAGATGCATTACGCGCGCCGCGGCATCATCACGCCCGAGATGGAATATGTCGCGTTG
CGTGAATCGCTGAACCTGCAGGCGCTGCAAGACAAGCCCGAGTACCGCAAGCTGCTCAAGCAGCATCCGGGCTTCTCGTA
CGGCGCCAACCTGCCGCAGCGCCCGGAAGACATCACGCCGGAATTCGTGCGCCAGGAAATCGCCGCCGGCCGCGCCATCA
TCCCGGCCAACATCAACCACGTGGAGCTGGAGCCGATGGCCATCGGCCGCAACTTCCGCGTGAAGATCAACGGCAACCTG
GGCAACTCGGCGGTCACCTCGTCGCTGGCCGAGGAAGTGGAAAAGATGGTGTGGTCGATCCGCTGGGGCGCCGACACGAT
CATGGATCTGTCCACCGGCAAGCACATCCACGAGACGCGCGAATGGATCCTGCGCAACTCGCCGGTGCCCATCGGCACGG
TGCCGATCTACCAGGCGCTGGACAAGACCGGCGGCGTGGCCGAAGACCTGACGTGGGAGATGTTCCGCGACACCCTCATC
GAGCAGGCCGAGCAGGGCGTGGACTACTTCACCATCCACGCCGGCGTGCTGCTGCGCTATGTGCCGCTCACGGCCGACCG
CATCACCGGCATCGTCTCGCGCGGCGGCTCGATCATGGCCAAGTGGTGCCTGGCGCATCACAAGGAGAACTTCCTGTACA
CGCACTTCGACGAGATCTGCGAAATCATGAAGGCGTACGACGTGTCGTTCAGCCTGGGTGACGGCCTGCGCCCGGGCTGC
ATCGCCGACTCGAACGACGCCGCGCAGTTCGGCGAGCTGTACACGCTGGGCGAGCTGACCAAGAAGGCGTGGGAGCACGA
CGTGCAGGTCATGATCGAAGGCCCGGGCCACGTGCCGCTGCAGCGCGTGCAGGCCAACATGGACGAAGAGCTCAAGCACT
GCTTCGAGGCCCCGTTCTACACGCTGGGCCCGCTGGTGACCGACATCGCTCCGGGCTACGACCACATCACCAGCGGCATC
GGCGCGGCCAACATCGGCTGGTACGGCACCGCCATGCTGTGCTACGTCACGCCCAAGGAGCACCTGGGCCTGCCGGACAA
GGAAGACGTGCGCGAAGGCATCATCACCTACAAGATCGCCGCGCATGCGGCCGACCTTGCGAAGGGCTGGCCGGGCGCGC
AGCTGCGCGACAACGCGCTGTCGAAGGCGCGTTTCGAGTTCCGCTGGGAAGACCAGTTCAACCTCGGCCTCGACCCGGAA
AAGGCGCGCGCCTTCCACGACGAGACCCTGCCGGCCGAAGGCGCCAAGATCGCCCACTTCTGCTCGATGTGCGGGCCGAA
ATTCTGCTCGATGAAGATCACGCAGGAAGTGCGCGACTACGCCGCCTCGCTCGACGCGAACGCCACCGCCGCGGAGCATG
GCATGGAAGAAAAGTCGATCGAGTTCCGCAAGGCCGGCGCAAAGATTTACAGCTGA

Upstream 100 bases:

>100_bases
AACACCGTGTACTTGGCAGCCCGTCCTTCCGGGCCTCGCCGCTCCCTCCCGGTTTCGTCCACAGCCTGCCCCGCCAGGCC
ACAGAATCAAGGACGAGACC

Downstream 100 bases:

>100_bases
CACAACCGATGCAATGCCAACCCCATTTGACGTAACGATCCTCGGCGGCGGCCTCGCTGGCCGCCTGTGTGCATGGCAAC
TCGCGCAGACGGGGGTACCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MPQAKNAPAASFDSLQTELDQKFAYPASSKTYIAGSRPDLRVPMRTIKQTATRTDQGEMLNPPIPVYDTSGPYSDPDVHI
DLKAGLAPLRAKWIDERGDTVVLPSLSSEYGQARAHDPATAHLRFAQLTNPRRAKPGANVSQMHYARRGIITPEMEYVAL
RESLNLQALQDKPEYRKLLKQHPGFSYGANLPQRPEDITPEFVRQEIAAGRAIIPANINHVELEPMAIGRNFRVKINGNL
GNSAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGVAEDLTWEMFRDTLI
EQAEQGVDYFTIHAGVLLRYVPLTADRITGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGC
IADSNDAAQFGELYTLGELTKKAWEHDVQVMIEGPGHVPLQRVQANMDEELKHCFEAPFYTLGPLVTDIAPGYDHITSGI
GAANIGWYGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPE
KARAFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLDANATAAEHGMEEKSIEFRKAGAKIYS

Sequences:

>Translated_631_residues
MPQAKNAPAASFDSLQTELDQKFAYPASSKTYIAGSRPDLRVPMRTIKQTATRTDQGEMLNPPIPVYDTSGPYSDPDVHI
DLKAGLAPLRAKWIDERGDTVVLPSLSSEYGQARAHDPATAHLRFAQLTNPRRAKPGANVSQMHYARRGIITPEMEYVAL
RESLNLQALQDKPEYRKLLKQHPGFSYGANLPQRPEDITPEFVRQEIAAGRAIIPANINHVELEPMAIGRNFRVKINGNL
GNSAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGVAEDLTWEMFRDTLI
EQAEQGVDYFTIHAGVLLRYVPLTADRITGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGC
IADSNDAAQFGELYTLGELTKKAWEHDVQVMIEGPGHVPLQRVQANMDEELKHCFEAPFYTLGPLVTDIAPGYDHITSGI
GAANIGWYGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPE
KARAFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLDANATAAEHGMEEKSIEFRKAGAKIYS
>Mature_630_residues
PQAKNAPAASFDSLQTELDQKFAYPASSKTYIAGSRPDLRVPMRTIKQTATRTDQGEMLNPPIPVYDTSGPYSDPDVHID
LKAGLAPLRAKWIDERGDTVVLPSLSSEYGQARAHDPATAHLRFAQLTNPRRAKPGANVSQMHYARRGIITPEMEYVALR
ESLNLQALQDKPEYRKLLKQHPGFSYGANLPQRPEDITPEFVRQEIAAGRAIIPANINHVELEPMAIGRNFRVKINGNLG
NSAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGVAEDLTWEMFRDTLIE
QAEQGVDYFTIHAGVLLRYVPLTADRITGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCI
ADSNDAAQFGELYTLGELTKKAWEHDVQVMIEGPGHVPLQRVQANMDEELKHCFEAPFYTLGPLVTDIAPGYDHITSGIG
AANIGWYGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPEK
ARAFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLDANATAAEHGMEEKSIEFRKAGAKIYS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=612, Percent_Identity=69.7712418300653, Blast_Score=892, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_RALSO (Q8Y368)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518234.1
- GeneID:   1218916
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0113
- NMPDR:   fig|267608.1.peg.113
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   RSOL267608:RSC0113-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 70091; Mature: 69960

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: BINDING 239-239 BINDING 268-268 BINDING 297-297 BINDING 333-333 BINDING 433-433 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQAKNAPAASFDSLQTELDQKFAYPASSKTYIAGSRPDLRVPMRTIKQTATRTDQGEML
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCC
NPPIPVYDTSGPYSDPDVHIDLKAGLAPLRAKWIDERGDTVVLPSLSSEYGQARAHDPAT
CCCCCCEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCCHHCCCCCCCCHH
AHLRFAQLTNPRRAKPGANVSQMHYARRGIITPEMEYVALRESLNLQALQDKPEYRKLLK
HEEEEHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHH
QHPGFSYGANLPQRPEDITPEFVRQEIAAGRAIIPANINHVELEPMAIGRNFRVKINGNL
HCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCEEEECEEEECCEEEEEECCCC
GNSAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
DKTGGVAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTADRITGIVSRGGSIMA
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHH
KWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDAAQFGELYTLGELT
HHHHHHHCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KKAWEHDVQVMIEGPGHVPLQRVQANMDEELKHCFEAPFYTLGPLVTDIAPGYDHITSGI
HHHHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCC
GAANIGWYGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNAL
CCCCCCCEEEEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLGLDPEKARAFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDY
HHCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
AASLDANATAAEHGMEEKSIEFRKAGAKIYS
HHHCCCCCHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PQAKNAPAASFDSLQTELDQKFAYPASSKTYIAGSRPDLRVPMRTIKQTATRTDQGEML
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCC
NPPIPVYDTSGPYSDPDVHIDLKAGLAPLRAKWIDERGDTVVLPSLSSEYGQARAHDPAT
CCCCCCEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCCHHCCCCCCCCHH
AHLRFAQLTNPRRAKPGANVSQMHYARRGIITPEMEYVALRESLNLQALQDKPEYRKLLK
HEEEEHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHH
QHPGFSYGANLPQRPEDITPEFVRQEIAAGRAIIPANINHVELEPMAIGRNFRVKINGNL
HCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCEEEECEEEECCEEEEEECCCC
GNSAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
DKTGGVAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTADRITGIVSRGGSIMA
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHH
KWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDAAQFGELYTLGELT
HHHHHHHCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KKAWEHDVQVMIEGPGHVPLQRVQANMDEELKHCFEAPFYTLGPLVTDIAPGYDHITSGI
HHHHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCC
GAANIGWYGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNAL
CCCCCCCEEEEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLGLDPEKARAFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDY
HHCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
AASLDANATAAEHGMEEKSIEFRKAGAKIYS
HHHCCCCCHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852