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The map label for this gene is rrmB [H]

Identifier: 17544794

GI number: 17544794

Start: 85330

End: 86655

Strand: Direct

Name: rrmB [H]

Synonym: RSc0075

Alternate gene names: 17544794

Gene position: 85330-86655 (Clockwise)

Preceding gene: 17544793

Following gene: 17544795

Centisome position: 2.3

GC content: 71.49

Gene sequence:

>1326_bases
ATGCGTCTGCCGCCCGATTCCCTCGCCCATGCCCTGTCGCTCGCCGCTGCCGTCATCGGCCGGTTGCGGCAGGGGACGGC
GCTGCCGCAGGCCATCGATGCCGCATGCCCGGGCCAGCCCGGTGCCACGCGTGGCGCGGTGCAGGATCTCGCCTATCGCG
CCACACGCTGGCTCGGCAGCGCCGATTGGCTGATCCGCACGCTGATTCCGCGCCCGCCCGCCGAGGGTGCGGCCAACCTG
TTGCGCGTGGCGCTGGCTCAGCTGCTCGACACGCCGCAGCCCTATGCACCTTTCACGGTCGTCGATCAGACCGTGCGTGC
TGCCTCGGCCGACCCGAAGCTCGCGCACGCCAAGGGCATGATCAACGGGGTGTTGCGGCGTTTCCTGCGGGAGCAGGCGG
ACCTGACGGCCGCCATGCAGGCCGATCCGGTCGCGGCGATCAACTATCCGGCCTGGTGGATCGGTGCCGTGCGCGAGGCC
TACCCCGAGGCGTGGCAGGCCATCCTGGCGGCCGGCAACCGTCGTCCGCCGATGGTGTTGCGCGTCAATCCGAGCCGCGT
GCCGGTCGAGGCCTACCTCGCGCGGCTGGCCGAGGTCGGCATCGAGGCCGAACGCATCGGCGCGCAGGCCGTGCGCCTGG
CGCGCGCGATGCCGGTGGGCGATCTGCCCGGCTTCGCCGAGGGCGATGTCTCGGTGCAGGATGCGGGCGCGCAACTCGCG
GCGGCGCTGCTCGATGTGGCGCCCGGCCAGCGTGTGCTGGATGCCTGCGCCGCGCCGGGCGGCAAGACCGGCCATCTGCT
GGAGCTCGCCGACGGCCTGGACGTGGTCGCGCTCGAATCCGATGCCGCGCGCGCCGCGCGCATCGGCGAGAACCTGGCGC
GGCTGGGGCAGACCGCCACCGTTTGCGTGGGCGACGCGGCCAAGCCCGATGCATGGTGGGACGGCCGCCCGTTCGACCGC
ATCCTGGTCGACGTGCCGTGCTCCGCGGCGGGCATCGTGCGGCGTCATCCGGACATCCGCTGGCTGCGCCGTCCGGCCGA
TCTGCCTGCGCTGGCCGCGCTGCAGCGGGACATCGTGAGCGCGCTATGGGCATGCCTGAAGCCCGGTGGCAAGCTGGTTT
ATGTCACCTGTTCGATTTTCCCGACGGAAGGCGAGGCCCAGGCCCGATGGTTTGAAAGCCGTCTGCAAGATGCGATACGA
TTGCACGCGCCCGGCCAGTTGCTGCCGGCCATGCCCGAGGCGGCTGGCGGCTTCGTTCCTGGCGTCTCATCCCTGCCGCT
CGACCACGACGGCTTCTATTACGCGGTGTTCCAGAAACGGCCCTGA

Upstream 100 bases:

>100_bases
TCCGGCCTGAGCCCGCGCTTCCTTTATACTTGCGCCGTCCGCACGAGCCCGCCCACCACGGGCTCGTTGCTTTTCCACCG
TCCGCTTTCCGCCGTCGTCG

Downstream 100 bases:

>100_bases
TGCGACGACCGTTGTGACTGCACTCCTTCGCCCATCGACCTGGCTGCCGGCGGTGCCCCGGTTCCGGCAAGTTCTGCATC
GGCTGGTCGTGCTGGGCATG

Product: RNA methyltransferase (SUN protein)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MRLPPDSLAHALSLAAAVIGRLRQGTALPQAIDAACPGQPGATRGAVQDLAYRATRWLGSADWLIRTLIPRPPAEGAANL
LRVALAQLLDTPQPYAPFTVVDQTVRAASADPKLAHAKGMINGVLRRFLREQADLTAAMQADPVAAINYPAWWIGAVREA
YPEAWQAILAAGNRRPPMVLRVNPSRVPVEAYLARLAEVGIEAERIGAQAVRLARAMPVGDLPGFAEGDVSVQDAGAQLA
AALLDVAPGQRVLDACAAPGGKTGHLLELADGLDVVALESDAARAARIGENLARLGQTATVCVGDAAKPDAWWDGRPFDR
ILVDVPCSAAGIVRRHPDIRWLRRPADLPALAALQRDIVSALWACLKPGGKLVYVTCSIFPTEGEAQARWFESRLQDAIR
LHAPGQLLPAMPEAAGGFVPGVSSLPLDHDGFYYAVFQKRP

Sequences:

>Translated_441_residues
MRLPPDSLAHALSLAAAVIGRLRQGTALPQAIDAACPGQPGATRGAVQDLAYRATRWLGSADWLIRTLIPRPPAEGAANL
LRVALAQLLDTPQPYAPFTVVDQTVRAASADPKLAHAKGMINGVLRRFLREQADLTAAMQADPVAAINYPAWWIGAVREA
YPEAWQAILAAGNRRPPMVLRVNPSRVPVEAYLARLAEVGIEAERIGAQAVRLARAMPVGDLPGFAEGDVSVQDAGAQLA
AALLDVAPGQRVLDACAAPGGKTGHLLELADGLDVVALESDAARAARIGENLARLGQTATVCVGDAAKPDAWWDGRPFDR
ILVDVPCSAAGIVRRHPDIRWLRRPADLPALAALQRDIVSALWACLKPGGKLVYVTCSIFPTEGEAQARWFESRLQDAIR
LHAPGQLLPAMPEAAGGFVPGVSSLPLDHDGFYYAVFQKRP
>Mature_441_residues
MRLPPDSLAHALSLAAAVIGRLRQGTALPQAIDAACPGQPGATRGAVQDLAYRATRWLGSADWLIRTLIPRPPAEGAANL
LRVALAQLLDTPQPYAPFTVVDQTVRAASADPKLAHAKGMINGVLRRFLREQADLTAAMQADPVAAINYPAWWIGAVREA
YPEAWQAILAAGNRRPPMVLRVNPSRVPVEAYLARLAEVGIEAERIGAQAVRLARAMPVGDLPGFAEGDVSVQDAGAQLA
AALLDVAPGQRVLDACAAPGGKTGHLLELADGLDVVALESDAARAARIGENLARLGQTATVCVGDAAKPDAWWDGRPFDR
ILVDVPCSAAGIVRRHPDIRWLRRPADLPALAALQRDIVSALWACLKPGGKLVYVTCSIFPTEGEAQARWFESRLQDAIR
LHAPGQLLPAMPEAAGGFVPGVSSLPLDHDGFYYAVFQKRP

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=288, Percent_Identity=27.7777777777778, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI76150623, Length=288, Percent_Identity=27.7777777777778, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI32698918, Length=172, Percent_Identity=35.4651162790698, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI39995082, Length=341, Percent_Identity=25.5131964809384, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI301336155, Length=226, Percent_Identity=29.646017699115, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI40316918, Length=168, Percent_Identity=32.7380952380952, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI23199998, Length=234, Percent_Identity=32.9059829059829, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI270288816, Length=234, Percent_Identity=32.9059829059829, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI270288818, Length=230, Percent_Identity=33.4782608695652, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI8922322, Length=234, Percent_Identity=32.9059829059829, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI2367212, Length=433, Percent_Identity=41.108545034642, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI87081985, Length=313, Percent_Identity=29.7124600638978, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17536757, Length=335, Percent_Identity=25.3731343283582, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25143471, Length=203, Percent_Identity=32.512315270936, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319447, Length=309, Percent_Identity=28.8025889967638, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=286, Percent_Identity=27.6223776223776, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI22024126, Length=175, Percent_Identity=32.5714285714286, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355201, Length=240, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24668781, Length=240, Percent_Identity=32.9166666666667, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356513, Length=240, Percent_Identity=32.9166666666667, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19920494, Length=165, Percent_Identity=30.3030303030303, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46896; Mature: 46896

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPPDSLAHALSLAAAVIGRLRQGTALPQAIDAACPGQPGATRGAVQDLAYRATRWLGS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
ADWLIRTLIPRPPAEGAANLLRVALAQLLDTPQPYAPFTVVDQTVRAASADPKLAHAKGM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
INGVLRRFLREQADLTAAMQADPVAAINYPAWWIGAVREAYPEAWQAILAAGNRRPPMVL
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
RVNPSRVPVEAYLARLAEVGIEAERIGAQAVRLARAMPVGDLPGFAEGDVSVQDAGAQLA
EECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
AALLDVAPGQRVLDACAAPGGKTGHLLELADGLDVVALESDAARAARIGENLARLGQTAT
HHHHHCCCCHHHHHHHCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCEE
VCVGDAAKPDAWWDGRPFDRILVDVPCSAAGIVRRHPDIRWLRRPADLPALAALQRDIVS
EEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHH
ALWACLKPGGKLVYVTCSIFPTEGEAQARWFESRLQDAIRLHAPGQLLPAMPEAAGGFVP
HHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHCCCCCC
GVSSLPLDHDGFYYAVFQKRP
CCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MRLPPDSLAHALSLAAAVIGRLRQGTALPQAIDAACPGQPGATRGAVQDLAYRATRWLGS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
ADWLIRTLIPRPPAEGAANLLRVALAQLLDTPQPYAPFTVVDQTVRAASADPKLAHAKGM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
INGVLRRFLREQADLTAAMQADPVAAINYPAWWIGAVREAYPEAWQAILAAGNRRPPMVL
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
RVNPSRVPVEAYLARLAEVGIEAERIGAQAVRLARAMPVGDLPGFAEGDVSVQDAGAQLA
EECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
AALLDVAPGQRVLDACAAPGGKTGHLLELADGLDVVALESDAARAARIGENLARLGQTAT
HHHHHCCCCHHHHHHHCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCEE
VCVGDAAKPDAWWDGRPFDRILVDVPCSAAGIVRRHPDIRWLRRPADLPALAALQRDIVS
EEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHH
ALWACLKPGGKLVYVTCSIFPTEGEAQARWFESRLQDAIRLHAPGQLLPAMPEAAGGFVP
HHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHCCCCCC
GVSSLPLDHDGFYYAVFQKRP
CCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA