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The map label for this gene is gatA [H]

Identifier: 17544776

GI number: 17544776

Start: 62121

End: 63608

Strand: Reverse

Name: gatA [H]

Synonym: RSc0057

Alternate gene names: 17544776

Gene position: 63608-62121 (Counterclockwise)

Preceding gene: 17544777

Following gene: 17544775

Centisome position: 1.71

GC content: 70.36

Gene sequence:

>1488_bases
ATGACCGCATCCACGCTCAAAACCCTGTCCGCCCAACTCGCCGCCAAGGAAGTGTCGGCGGTGGAACTGGCGCGCCACTA
TCTGGCGCGCATCGAGGCGCGGGCCGACCTGAACGCCTTCATCCATGTCGATCCCGAGGCCACGCTGGCGCAGGCGCAAG
CCGCCGACGCGCGCCTGGCCGCCGGCGACGCGGGGCCGCTCGCCGGTATCCCGATCGCGCACAAGGACGTGTTCGTCACG
CGCGGCTGGCGCGCCACGGCCGGCTCGAAGATGCTCGACAGCTACGTGAGCCCGTTCGACGCCACCGTGGTCGAGCGCCT
GGCCGCCGCCGGCATGGTGACGCTGGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCCAACGAGAACTCGCATT
TCGGCCCGGTCAAGAATCCGTGGGATGTCGCGCGCGTGCCGGGCGGCTCGTCCGGCGGCTCGGCCGCAGCCGTGGCGGCG
GACCTGGCGCCGGCCGCGACCGGCACCGACACCGGCGGCTCGATCCGCCAGCCGGCGTCGTTCTCGGGCATCACCGGCAT
CAAGCCGACGTATGGCCGGGTGTCGCGCTACGGCATGATCGCCTTTGCCTCGTCGCTGGACCAGGGCGGCCCGATGGCGC
GCACGGCCGAGGACTGCGCGCTGCTGCTGTCGGCCATGGCCGGCTTCGACGCGCGCGATTCCACCAGCCTCGAGCCGGGC
CGCGGCGGCGACGCGGAAGACTTCGGCCGCCTGCTAGGCCGGCCGCTGGAAGGCGCCGACGCCGCCCGCCCGCTGGCCGG
CCTGCGCATCGGGCTGCCGCAGGAATACTTCGGCGCAGGGCTGGCCGACGACGTGCGCACCGCCGTACGGGCCGCGCTGG
CCGAGTTGGAGACGCTGGGCGCTACGCTGGTCGACATCAGCCTGCCCAAGACCGAACTGTCGATCCCGACCTACTACGTG
ATCGCCCCGGCCGAGGCCTCGTCCAACCTGTCGCGCTTCGACGGCGTGCGCTACGGCCACCGCGCGGCGGCGTACCGCGA
CCTGGCCGACATGTACCGCAAGACCCGCGCCGAAGGCTTCGGCTGGGAGGTCAAGCGCCGCATCCTGGTCGGCACCTATG
TGCTGTCGCACGGCTACTACGACGCCTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGCAGGATTTCCAGAAC
GCGTTCGGGCAATGCGATGTGATCATGGGGCCGGTGGCGCCGACGGTGGCCTGGAAGCTCGGCGAGAAGACCGACGATCC
GCTGCAGATGTACCTGGCCGACATCTTCACGCTATCGACCAGCCTGGCCGGCCTGCCGGGCATGAGCGTGCCGGCCGGCT
TCGGCGCAAACGGTCTCCCGGTTGGCTTGCAGATCATTGGCAACTATTTTGAAGAGGCACGCATGCTGCAGATCGCGCAT
GCGTTCCAGCAGGCGACCGACTGGCACACGCGCCGGCCGGCGGCCTGA

Upstream 100 bases:

>100_bases
ATCGAGTAACGCATCGCCCCGAGGCGCCGACGCGCCTGCCCCGACCCCGTCCCATTTGCCGTCGCCGGCCGCTGCCGGCA
GCCCATCCGAACCGTATTCG

Downstream 100 bases:

>100_bases
CCCCGGGGTCCGGCGGCTCAACGCAATGGCGGAGGCGATCATGGCGCGACGCATCGGCATGGGCGGGTTGGCGGCATTGT
CCGCCGTGGTGCTGACCGCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTASTLKTLSAQLAAKEVSAVELARHYLARIEARADLNAFIHVDPEATLAQAQAADARLAAGDAGPLAGIPIAHKDVFVT
RGWRATAGSKMLDSYVSPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSHFGPVKNPWDVARVPGGSSGGSAAAVAA
DLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARTAEDCALLLSAMAGFDARDSTSLEPG
RGGDAEDFGRLLGRPLEGADAARPLAGLRIGLPQEYFGAGLADDVRTAVRAALAELETLGATLVDISLPKTELSIPTYYV
IAPAEASSNLSRFDGVRYGHRAAAYRDLADMYRKTRAEGFGWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQN
AFGQCDVIMGPVAPTVAWKLGEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGNYFEEARMLQIAH
AFQQATDWHTRRPAA

Sequences:

>Translated_495_residues
MTASTLKTLSAQLAAKEVSAVELARHYLARIEARADLNAFIHVDPEATLAQAQAADARLAAGDAGPLAGIPIAHKDVFVT
RGWRATAGSKMLDSYVSPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSHFGPVKNPWDVARVPGGSSGGSAAAVAA
DLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARTAEDCALLLSAMAGFDARDSTSLEPG
RGGDAEDFGRLLGRPLEGADAARPLAGLRIGLPQEYFGAGLADDVRTAVRAALAELETLGATLVDISLPKTELSIPTYYV
IAPAEASSNLSRFDGVRYGHRAAAYRDLADMYRKTRAEGFGWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQN
AFGQCDVIMGPVAPTVAWKLGEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGNYFEEARMLQIAH
AFQQATDWHTRRPAA
>Mature_494_residues
TASTLKTLSAQLAAKEVSAVELARHYLARIEARADLNAFIHVDPEATLAQAQAADARLAAGDAGPLAGIPIAHKDVFVTR
GWRATAGSKMLDSYVSPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSHFGPVKNPWDVARVPGGSSGGSAAAVAAD
LAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARTAEDCALLLSAMAGFDARDSTSLEPGR
GGDAEDFGRLLGRPLEGADAARPLAGLRIGLPQEYFGAGLADDVRTAVRAALAELETLGATLVDISLPKTELSIPTYYVI
APAEASSNLSRFDGVRYGHRAAAYRDLADMYRKTRAEGFGWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNA
FGQCDVIMGPVAPTVAWKLGEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGNYFEEARMLQIAHA
FQQATDWHTRRPAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=513, Percent_Identity=37.6218323586745, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI195972892, Length=505, Percent_Identity=25.9405940594059, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI166795287, Length=160, Percent_Identity=38.125, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=39.3805309734513, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=25.8, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=193, Percent_Identity=32.6424870466321, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=196, Percent_Identity=31.6326530612245, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=30.1310043668122, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=32.8125, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=166, Percent_Identity=34.9397590361446, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=192, Percent_Identity=30.2083333333333, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=443, Percent_Identity=34.9887133182844, Blast_Score=243, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=454, Percent_Identity=27.7533039647577, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=241, Percent_Identity=29.045643153527, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.1836734693878, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=26.8244575936884, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=26.8244575936884, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=26.8244575936884, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=26.8244575936884, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=27.0541082164329, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078093, Length=528, Percent_Identity=26.8939393939394, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648435, Length=510, Percent_Identity=24.5098039215686, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648437, Length=510, Percent_Identity=24.5098039215686, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550774, Length=233, Percent_Identity=32.1888412017167, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648441, Length=444, Percent_Identity=25.4504504504505, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648439, Length=444, Percent_Identity=25.4504504504505, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356731, Length=232, Percent_Identity=31.4655172413793, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52357; Mature: 52226

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTLKTLSAQLAAKEVSAVELARHYLARIEARADLNAFIHVDPEATLAQAQAADARLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEE
AGDAGPLAGIPIAHKDVFVTRGWRATAGSKMLDSYVSPFDATVVERLAAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSHFGPVKNPWDVARVPGGSSGGSAAAVAADLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARTAEDCALLLSAMAGFDARDSTSLEPG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
RGGDAEDFGRLLGRPLEGADAARPLAGLRIGLPQEYFGAGLADDVRTAVRAALAELETLG
CCCCHHHHHHHHCCCCCCCCHHCCHHCCEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHC
ATLVDISLPKTELSIPTYYVIAPAEASSNLSRFDGVRYGHRAAAYRDLADMYRKTRAEGF
CEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
GWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFGQCDVIMGPVAPTVAWKL
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHC
GEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGNYFEEARMLQIAH
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AFQQATDWHTRRPAA
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
TASTLKTLSAQLAAKEVSAVELARHYLARIEARADLNAFIHVDPEATLAQAQAADARLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEE
AGDAGPLAGIPIAHKDVFVTRGWRATAGSKMLDSYVSPFDATVVERLAAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSHFGPVKNPWDVARVPGGSSGGSAAAVAADLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARTAEDCALLLSAMAGFDARDSTSLEPG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
RGGDAEDFGRLLGRPLEGADAARPLAGLRIGLPQEYFGAGLADDVRTAVRAALAELETLG
CCCCHHHHHHHHCCCCCCCCHHCCHHCCEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHC
ATLVDISLPKTELSIPTYYVIAPAEASSNLSRFDGVRYGHRAAAYRDLADMYRKTRAEGF
CEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
GWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFGQCDVIMGPVAPTVAWKL
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHC
GEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGNYFEEARMLQIAH
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AFQQATDWHTRRPAA
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA