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The map label for this gene is gsiB [H]

Identifier: 17544763

GI number: 17544763

Start: 47608

End: 49200

Strand: Reverse

Name: gsiB [H]

Synonym: RSc0044

Alternate gene names: 17544763

Gene position: 49200-47608 (Counterclockwise)

Preceding gene: 17544764

Following gene: 17544762

Centisome position: 1.32

GC content: 69.11

Gene sequence:

>1593_bases
ATGCTGGTCCTGCGTCTGCTGCGCGCCGCGAGTGCGGTCGCCACGCTGTCCCTTGCCATGGCACTCGCACCCGCCGCGGC
CCACGCGCAGCCGCCGTCCACGCTGGTGGTCGGCCTGTCGGGCGACATCACCTCGCTCGACCCGCACTTCCACAACGTCA
CCCCCAACGCGAACGTCGCCGAGCACCTGTTCGAGACGCTGGTCGCCAAGGACGACAAGATGCGCATGCGGCCCGGCCTG
GCGATCTCCTGGCGGGCCGTCAGCGACACCGTGTGGGAGCTCAAGCTGCGCCCCGGCGTGCAGTTCCACGACGGCAGCGA
ATTCACCTCGGCCGACGTGGTGTACTCGCTGGCCCGGCCGGCCACCATCAAGAACAGCCCGTCGCCGTACACCATCTACA
CACGCGGCTTCAAGGACGTCACGGCGCTCGACAAGCTGACCGTGCGCATCACCACCAACGGACCGTACCCGCTGGTGCCG
AACGACCTGTCGACCCTCTACATCGTGTCGAAGAAGGCCGCCGAGAAAGCCGGCCCCGACGATTTCAACAACGGCCGCGC
GCTGATCGGCACCGGACCGTACAAGTTCGTCTCGTTCACCAAGGGCGACCGCTTGGAGCTGGCGCGCAACGACGCCTACT
GGGGCAAGAAGCCGCAATGGGATCGCGTGACGCTGCGCATCCTGACCGCCGACGCCTCGCGTGTGGCCGCGCTGCTGGCC
GGCGACGTGCAGGTCATCGAGAGCGTGCCGCCGCCCGACATCAAAAGGCTGGCGGCCAATCCGGCGGTCTCGGTGTTCAA
GATGCCGGCGTTCCGCCTGATGTACCTGCACATGGATTCGGCGCGCGAGGTCTCGCCGTTCGTGACCGACAAGGCCGGCC
GGCCGCTGGCGAAGAACCCGCTCAAGGACGTGCGCGTGCGCCAGGCGATCTCGCTGGCGATCTCGCGCCAGGCCATCGCC
GACCACGTCATGGAAGGCGCGGCCGAGCCGACCGGTCAGCTCATCAACAGCACGCTGTTCGGCCACGTGCCCGGCCTGAA
GGCGCCGGTGGCCGACGTGGCCGCCGCGAAGACACTGCTGGCCCAGGCCGGCTACCCCGACGGCTTCGGCATCACGCTGC
ACGCCACCAACAACCGCTACGTCAACGACCACCGCGTCGCGCAGGCGATCGCGTCGATGCTCACGCGCGTGGGCATCGCC
ACCCGGGTGGAGACCATGCCGTCGGCCACGTTCTTCATGCGCGCCAACAAGCTGGAGTTCTCGTTCCTGCTGGCCGGCTG
GGGCGCGGATACGGGCGAGGCCAGCTCCTGCCTGAAGGCCCTGCTGGCCACCTACGACCCGGCGCGCGGCTGGGGCGCCT
CCAACCGGGGCCGCTATTCCAGCCCCGCGCTCGACGCCAAGCTGGCCGAGGCCCTGCAGACGCTCGACGACGCCAGGCGC
GAGAAGCTGCTGCAGGAGGCGACGGAGATCGGCATGCGCGAGGTCGGGGTGATTCCGCTCTACTTCAACATCAACGCCTG
GGCGGCGCGCAAGGGCTACACGGTGGTGCCGCACCTGGACGACCGCACCCACGCCTTCGAGGTGACGCGCTAA

Upstream 100 bases:

>100_bases
ATCGGGGTTGCCCCCGCTTCACCAGCATTGACACCGGTGTCGCCCGGTCCCGACAATCCCCCGAGCGCTTGAACACGTTT
TAACATTTCGGGGAGTCGTC

Downstream 100 bases:

>100_bases
CCGCATCGAAGCGGCCCATGGCGCACAAATCCACGCGCGGCGCACGGGAATCGAGGCTTGAAATCCTGACGGCCGGCGTC
AAGTATGGGACGCTTTGACC

Product: substrate-binding periplasmic (PBP) ABC transporter protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLVLRLLRAASAVATLSLAMALAPAAAHAQPPSTLVVGLSGDITSLDPHFHNVTPNANVAEHLFETLVAKDDKMRMRPGL
AISWRAVSDTVWELKLRPGVQFHDGSEFTSADVVYSLARPATIKNSPSPYTIYTRGFKDVTALDKLTVRITTNGPYPLVP
NDLSTLYIVSKKAAEKAGPDDFNNGRALIGTGPYKFVSFTKGDRLELARNDAYWGKKPQWDRVTLRILTADASRVAALLA
GDVQVIESVPPPDIKRLAANPAVSVFKMPAFRLMYLHMDSAREVSPFVTDKAGRPLAKNPLKDVRVRQAISLAISRQAIA
DHVMEGAAEPTGQLINSTLFGHVPGLKAPVADVAAAKTLLAQAGYPDGFGITLHATNNRYVNDHRVAQAIASMLTRVGIA
TRVETMPSATFFMRANKLEFSFLLAGWGADTGEASSCLKALLATYDPARGWGASNRGRYSSPALDAKLAEALQTLDDARR
EKLLQEATEIGMREVGVIPLYFNINAWAARKGYTVVPHLDDRTHAFEVTR

Sequences:

>Translated_530_residues
MLVLRLLRAASAVATLSLAMALAPAAAHAQPPSTLVVGLSGDITSLDPHFHNVTPNANVAEHLFETLVAKDDKMRMRPGL
AISWRAVSDTVWELKLRPGVQFHDGSEFTSADVVYSLARPATIKNSPSPYTIYTRGFKDVTALDKLTVRITTNGPYPLVP
NDLSTLYIVSKKAAEKAGPDDFNNGRALIGTGPYKFVSFTKGDRLELARNDAYWGKKPQWDRVTLRILTADASRVAALLA
GDVQVIESVPPPDIKRLAANPAVSVFKMPAFRLMYLHMDSAREVSPFVTDKAGRPLAKNPLKDVRVRQAISLAISRQAIA
DHVMEGAAEPTGQLINSTLFGHVPGLKAPVADVAAAKTLLAQAGYPDGFGITLHATNNRYVNDHRVAQAIASMLTRVGIA
TRVETMPSATFFMRANKLEFSFLLAGWGADTGEASSCLKALLATYDPARGWGASNRGRYSSPALDAKLAEALQTLDDARR
EKLLQEATEIGMREVGVIPLYFNINAWAARKGYTVVPHLDDRTHAFEVTR
>Mature_530_residues
MLVLRLLRAASAVATLSLAMALAPAAAHAQPPSTLVVGLSGDITSLDPHFHNVTPNANVAEHLFETLVAKDDKMRMRPGL
AISWRAVSDTVWELKLRPGVQFHDGSEFTSADVVYSLARPATIKNSPSPYTIYTRGFKDVTALDKLTVRITTNGPYPLVP
NDLSTLYIVSKKAAEKAGPDDFNNGRALIGTGPYKFVSFTKGDRLELARNDAYWGKKPQWDRVTLRILTADASRVAALLA
GDVQVIESVPPPDIKRLAANPAVSVFKMPAFRLMYLHMDSAREVSPFVTDKAGRPLAKNPLKDVRVRQAISLAISRQAIA
DHVMEGAAEPTGQLINSTLFGHVPGLKAPVADVAAAKTLLAQAGYPDGFGITLHATNNRYVNDHRVAQAIASMLTRVGIA
TRVETMPSATFFMRANKLEFSFLLAGWGADTGEASSCLKALLATYDPARGWGASNRGRYSSPALDAKLAEALQTLDDARR
EKLLQEATEIGMREVGVIPLYFNINAWAARKGYTVVPHLDDRTHAFEVTR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=536, Percent_Identity=28.1716417910448, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789966, Length=518, Percent_Identity=28.5714285714286, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1787495, Length=565, Percent_Identity=26.3716814159292, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=25.1396648044693, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787762, Length=533, Percent_Identity=24.765478424015, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=24.9056603773585, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789887, Length=498, Percent_Identity=23.2931726907631, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787551, Length=540, Percent_Identity=22.962962962963, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57519; Mature: 57519

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLRLLRAASAVATLSLAMALAPAAAHAQPPSTLVVGLSGDITSLDPHFHNVTPNANVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHH
EHLFETLVAKDDKMRMRPGLAISWRAVSDTVWELKLRPGVQFHDGSEFTSADVVYSLARP
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEECCCCCCCHHHHHHHHCCC
ATIKNSPSPYTIYTRGFKDVTALDKLTVRITTNGPYPLVPNDLSTLYIVSKKAAEKAGPD
CCCCCCCCCEEEEECCCCHHEEECEEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHCCCC
DFNNGRALIGTGPYKFVSFTKGDRLELARNDAYWGKKPQWDRVTLRILTADASRVAALLA
CCCCCEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHH
GDVQVIESVPPPDIKRLAANPAVSVFKMPAFRLMYLHMDSAREVSPFVTDKAGRPLAKNP
CCHHHHHCCCCHHHHHHHCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
LKDVRVRQAISLAISRQAIADHVMEGAAEPTGQLINSTLFGHVPGLKAPVADVAAAKTLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
AQAGYPDGFGITLHATNNRYVNDHRVAQAIASMLTRVGIATRVETMPSATFFMRANKLEF
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
SFLLAGWGADTGEASSCLKALLATYDPARGWGASNRGRYSSPALDAKLAEALQTLDDARR
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKLLQEATEIGMREVGVIPLYFNINAWAARKGYTVVPHLDDRTHAFEVTR
HHHHHHHHHCCHHHCCEEEEEEECCEEECCCCCEEECCCCCCCCEEEECC
>Mature Secondary Structure
MLVLRLLRAASAVATLSLAMALAPAAAHAQPPSTLVVGLSGDITSLDPHFHNVTPNANVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHH
EHLFETLVAKDDKMRMRPGLAISWRAVSDTVWELKLRPGVQFHDGSEFTSADVVYSLARP
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEECCCCCCCHHHHHHHHCCC
ATIKNSPSPYTIYTRGFKDVTALDKLTVRITTNGPYPLVPNDLSTLYIVSKKAAEKAGPD
CCCCCCCCCEEEEECCCCHHEEECEEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHCCCC
DFNNGRALIGTGPYKFVSFTKGDRLELARNDAYWGKKPQWDRVTLRILTADASRVAALLA
CCCCCEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHH
GDVQVIESVPPPDIKRLAANPAVSVFKMPAFRLMYLHMDSAREVSPFVTDKAGRPLAKNP
CCHHHHHCCCCHHHHHHHCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
LKDVRVRQAISLAISRQAIADHVMEGAAEPTGQLINSTLFGHVPGLKAPVADVAAAKTLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
AQAGYPDGFGITLHATNNRYVNDHRVAQAIASMLTRVGIATRVETMPSATFFMRANKLEF
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
SFLLAGWGADTGEASSCLKALLATYDPARGWGASNRGRYSSPALDAKLAEALQTLDDARR
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKLLQEATEIGMREVGVIPLYFNINAWAARKGYTVVPHLDDRTHAFEVTR
HHHHHHHHHCCHHHCCEEEEEEECCEEECCCCCEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA