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The map label for this gene is phoB [H]

Identifier: 17544737

GI number: 17544737

Start: 21645

End: 22484

Strand: Direct

Name: phoB [H]

Synonym: RSc0018

Alternate gene names: 17544737

Gene position: 21645-22484 (Clockwise)

Preceding gene: 17544736

Following gene: 17544738

Centisome position: 0.58

GC content: 69.52

Gene sequence:

>840_bases
GTGGGCATTCAGATCTTGCTGGTTGAACAGGACCAGGCTGAGGGGGAGCGACTGTCGTTGTCCCTGCGCGATGGCGGACA
CCACGCCCAGCGCGTCGACAGTCCGGCGCAGGCGCAGGCCGCGATGCAGGCATCGCAGCCGGACCTGCTGCTGATGGAGT
GGAACTGGCCGGAATGCGAGTCGATCGACATGCTGCTGGCCCTGCGTGCCAGCGCGCACACGCGCAGCCTGCCGGTCATC
GTGCTGTCGCGCCACGGCGACGCGCGCGCCAAGATCGCGGCACTCGATGCCGGCGCCGACGATTACGTGGTCAAGCCCTG
CGATGTGGGCGAGCTGCATGCCCGCATTCGTGCCGTGCTGCGCCGCCGCTCGCCGCAGCAGGGGGACGAGATCCTGCAGA
TCAACGGCCTGCGGCTTGACCCGCTCACGCTGGGCGTGACCGCGCAGACGGACGAGGGGCCGCGGCCGGTCGCGCTGAGC
CCGCTGGAATTCCGCCTGCTGCATTTCCTGGTGGCGCATCCGCAGCGCGTGCATTCGCGCACCCAACTGCTCGACCGCGT
CTGGGGCAACCAGGTCTTCGTGGGCGAACGCACGGTCGATGTGCACGTCCGCAAACTGCGCGTGGCGCTGGAGGGCACGC
TGTGCGATGGCCTGATCCAGACCGTGCGCGGCGGCGGTTATCGCCTGCTGGTCGGCAATGGTGTCCACCAGGGGCCATCC
GGCGAGGCGATCGACGCGCAATTCGAGGTCGCGGCGCGCGAGCATGATGAGTCTTCGGCTTACGAAGGCCCTTCGGCCCG
TACCCGCGCTGCCCGGGAGCCGGCCCGCCTGAACGCCTGA

Upstream 100 bases:

>100_bases
CCCGCATGGCTTCATAACAACGCGCACCGCAGACAGAAGAGCGCGGCCTCAGGCCGCTCGCCGGATATTTGTCGGCTGGG
TTAGACGGGAGGTTTTACAA

Downstream 100 bases:

>100_bases
GCTGAGCGCCGGTTTGGCCAACAAGACAGGATCGTATCGACAACGCCCTTCGCCTCGCAGGCCGCGAGATGAAGGGCGTT
GGCGTGTTTATTTGTGTCAA

Product: response regulator transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MGIQILLVEQDQAEGERLSLSLRDGGHHAQRVDSPAQAQAAMQASQPDLLLMEWNWPECESIDMLLALRASAHTRSLPVI
VLSRHGDARAKIAALDAGADDYVVKPCDVGELHARIRAVLRRRSPQQGDEILQINGLRLDPLTLGVTAQTDEGPRPVALS
PLEFRLLHFLVAHPQRVHSRTQLLDRVWGNQVFVGERTVDVHVRKLRVALEGTLCDGLIQTVRGGGYRLLVGNGVHQGPS
GEAIDAQFEVAAREHDESSAYEGPSARTRAAREPARLNA

Sequences:

>Translated_279_residues
MGIQILLVEQDQAEGERLSLSLRDGGHHAQRVDSPAQAQAAMQASQPDLLLMEWNWPECESIDMLLALRASAHTRSLPVI
VLSRHGDARAKIAALDAGADDYVVKPCDVGELHARIRAVLRRRSPQQGDEILQINGLRLDPLTLGVTAQTDEGPRPVALS
PLEFRLLHFLVAHPQRVHSRTQLLDRVWGNQVFVGERTVDVHVRKLRVALEGTLCDGLIQTVRGGGYRLLVGNGVHQGPS
GEAIDAQFEVAAREHDESSAYEGPSARTRAAREPARLNA
>Mature_278_residues
GIQILLVEQDQAEGERLSLSLRDGGHHAQRVDSPAQAQAAMQASQPDLLLMEWNWPECESIDMLLALRASAHTRSLPVIV
LSRHGDARAKIAALDAGADDYVVKPCDVGELHARIRAVLRRRSPQQGDEILQINGLRLDPLTLGVTAQTDEGPRPVALSP
LEFRLLHFLVAHPQRVHSRTQLLDRVWGNQVFVGERTVDVHVRKLRVALEGTLCDGLIQTVRGGGYRLLVGNGVHQGPSG
EAIDAQFEVAAREHDESSAYEGPSARTRAAREPARLNA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=41.0480349344978, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786784, Length=232, Percent_Identity=30.1724137931034, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786911, Length=209, Percent_Identity=33.0143540669856, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082012, Length=231, Percent_Identity=29.4372294372294, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790552, Length=232, Percent_Identity=32.3275862068966, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790860, Length=230, Percent_Identity=31.304347826087, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=29.6137339055794, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=29.8701298701299, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=25.3333333333333, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI2367329, Length=180, Percent_Identity=37.2222222222222, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=29.9559471365639, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=30.1310043668122, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=27.8481012658228, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 30563; Mature: 30432

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIQILLVEQDQAEGERLSLSLRDGGHHAQRVDSPAQAQAAMQASQPDLLLMEWNWPECE
CCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCC
SIDMLLALRASAHTRSLPVIVLSRHGDARAKIAALDAGADDYVVKPCDVGELHARIRAVL
CHHHHEEEHHCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEECCCCCHHHHHHHHHHH
RRRSPQQGDEILQINGLRLDPLTLGVTAQTDEGPRPVALSPLEFRLLHFLVAHPQRVHSR
HHCCCCCCCCEEEECCEEECCEEEEEEECCCCCCCCEEECHHHHHHHHHHHHCHHHHHHH
TQLLDRVWGNQVFVGERTVDVHVRKLRVALEGTLCDGLIQTVRGGGYRLLVGNGVHQGPS
HHHHHHHCCCEEEECCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
GEAIDAQFEVAAREHDESSAYEGPSARTRAAREPARLNA
CCEECCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
GIQILLVEQDQAEGERLSLSLRDGGHHAQRVDSPAQAQAAMQASQPDLLLMEWNWPECE
CEEEEEEECCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCC
SIDMLLALRASAHTRSLPVIVLSRHGDARAKIAALDAGADDYVVKPCDVGELHARIRAVL
CHHHHEEEHHCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEECCCCCHHHHHHHHHHH
RRRSPQQGDEILQINGLRLDPLTLGVTAQTDEGPRPVALSPLEFRLLHFLVAHPQRVHSR
HHCCCCCCCCEEEECCEEECCEEEEEEECCCCCCCCEEECHHHHHHHHHHHHCHHHHHHH
TQLLDRVWGNQVFVGERTVDVHVRKLRVALEGTLCDGLIQTVRGGGYRLLVGNGVHQGPS
HHHHHHHCCCEEEECCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
GEAIDAQFEVAAREHDESSAYEGPSARTRAAREPARLNA
CCEECCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]