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The map label for this gene is gyrB [H]

Identifier: 17232757

GI number: 17232757

Start: 6281429

End: 6283366

Strand: Reverse

Name: gyrB [H]

Synonym: all5265

Alternate gene names: 17232757

Gene position: 6283366-6281429 (Counterclockwise)

Preceding gene: 17232759

Following gene: 17232756

Centisome position: 97.97

GC content: 46.08

Gene sequence:

>1938_bases
ATGACGAGCAGTTACAGTGCCGATCAGATTCAAGTTCTGGAAGGTCTGGAAGCCGTCCGTAAACGACCGGGGATGTACAT
CGGTTCTACCGGGCCGCGAGGACTCCACCATCTAGTTTATGAGGTGGTAGACAACTCTATCGATGAAGCATTGGCAGGTT
ACTGCACTCATATAGAGGTGGAGATAAATGCCGATGGTTCCGTGGCTGTCACAGATGATGGGCGCGGTATTCCTATCGAT
ACTCACTCGCGGACGGGGAAATCCGCTTTGGAAACCGTGATGACAGTACTACACGCCGGGGGTAAGTTTGGCGGTGGTGG
TTACAAAGTTTCTGGAGGACTGCACGGGGTTGGTATTTCTGTAGTTAACGCCTTATCAGAAGTTGTAGAAGTTACAGTCT
GGCGAGATAAAAAAGTTCATACCCAGCGCTACGAACGAGGTGTTCCGGTTACTGAACTCATCACAAATCCTTATAAAGAA
GCGAGAACGGGAACATCTGTCACCTTCAAACCGGATACTCAAATCTTTACTAATAGTATTGAATTTGATTACATCACCCT
ATCAGGTCGCTTGCGTGAGTTGGCGTATCTGAATGCAGGAGTGAAGATTACTTTTACTGACAACCGTTTAGAACTGCTCA
AAAGCGATACACCTAAAGTAGAAAGCTACGAATACAAGGGTGGTATCAAAGAGTATATTGCTTACATGAACCGTGATAAG
CAACCACTACATGAAGAAATCATCTATGTGCAGGGAGAACGCAACAACGTACAGATAGAAGTCTCCTTGCAGTGGTGTAC
TGATGCTTATACAGATAACGTACTGGGTTTTGCCAATAATATCCGTACTATAGATGGCGGTACACACCTAGAAGGGTTGA
AGGCTGTTCTGACTCGCACATTAAATACGATCGCCCGCAAGCGCAATAAAATTAAAGAACATGAACCCAACCTTAGTGGT
GAACACGTCCGCGAAGGTTTAACCGCAGTCATTTCTGTTAAAGTCCCAGACCCAGAATTTGAAGGACAAACCAAAACCAA
ACTAGGTAACACTGAAGTCCGGGGTATTGTTGACTCTTTGGTAGGGGAAGTCCTCACCGAGTACTTAGAATTTCACCCTG
GTATCGCTGACTCAATTTTAGATAAAGCCATCCAAGCTTTCAAAGCCGCAGAAGCAGCACGCCACGCACGGGAGTTAGTG
CGACGTAAATCAGTACTAGAATCTTCCCCATTACCCGGTAAATTAGCAGATTGCAGTTCCCGCGACCCCAGCGAATCAGA
AATTTACATTGTGGAAGGTGACTCAGCCGGTGGTAGCGCCAAACAAGGACGTGATCGCCGCACCCAAGCCATCCTCCCCC
TACGTGGTAAAATCCTCAACATAGAAAAAACTGACGATTCCAAAATCTATAAAAATAATGAAATTCAGGCGTTAATTACC
GCCCTGGGTTTAGGAGTCAAAGGTGAAGAATTCGACTCCACCCAACTACGCTATCACCGCATCATCATCATGACCGATGC
TGACGTGGATGGGGCGCATATCCGCACATTAATATTGACCTTCTTTTATCGCTACCAGCGAGCGCTGATTGAACAAGGTT
TCATCTACATTGCTTGCCCCCCACTATATAAAGTAGAACGGGGACGCAATTATGAGTATTGTTACAGTGAGCGTGAACTA
CAACAAGCGATCGCCAAATTCCCAGCAAATGCCAACTACACCATTCAACGCTTCAAAGGTTTGGGTGAAATGATGCCAGA
ACAACTCTGGACAACCACTATGAATCCGGAAACTCGCACCCTCAAGCGAGTGGAAATCGAAGACGCAGCAGAGGCCGATC
GCATCTTTACAATTTTAATGGGCGATCGGGTTGCACCCAGACGCGAATTTATTGAAACCTACGGTTCTAAACTTAACCTC
ACGGATTTAGATATCTAA

Upstream 100 bases:

>100_bases
TGCCCTACAGTCGCCGTTAAGGCTTTTGTGTTAGAATTTTGGAGTGATTTGACTTTTTAGCCTTTGGGCGATTTCAACCC
CACGCATCAGGAGAATATTC

Downstream 100 bases:

>100_bases
TCGGTCACAAGTTTGAATATTTGAGATAACAATGGGTAATTGATTTTAAACAATTACCCATTCTCTTTACAAAACAAAAT
TCATACAAAACACACGCAGG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MTSSYSADQIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEVVDNSIDEALAGYCTHIEVEINADGSVAVTDDGRGIPID
THSRTGKSALETVMTVLHAGGKFGGGGYKVSGGLHGVGISVVNALSEVVEVTVWRDKKVHTQRYERGVPVTELITNPYKE
ARTGTSVTFKPDTQIFTNSIEFDYITLSGRLRELAYLNAGVKITFTDNRLELLKSDTPKVESYEYKGGIKEYIAYMNRDK
QPLHEEIIYVQGERNNVQIEVSLQWCTDAYTDNVLGFANNIRTIDGGTHLEGLKAVLTRTLNTIARKRNKIKEHEPNLSG
EHVREGLTAVISVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGEVLTEYLEFHPGIADSILDKAIQAFKAAEAARHARELV
RRKSVLESSPLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRTQAILPLRGKILNIEKTDDSKIYKNNEIQALIT
ALGLGVKGEEFDSTQLRYHRIIIMTDADVDGAHIRTLILTFFYRYQRALIEQGFIYIACPPLYKVERGRNYEYCYSEREL
QQAIAKFPANANYTIQRFKGLGEMMPEQLWTTTMNPETRTLKRVEIEDAAEADRIFTILMGDRVAPRREFIETYGSKLNL
TDLDI

Sequences:

>Translated_645_residues
MTSSYSADQIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEVVDNSIDEALAGYCTHIEVEINADGSVAVTDDGRGIPID
THSRTGKSALETVMTVLHAGGKFGGGGYKVSGGLHGVGISVVNALSEVVEVTVWRDKKVHTQRYERGVPVTELITNPYKE
ARTGTSVTFKPDTQIFTNSIEFDYITLSGRLRELAYLNAGVKITFTDNRLELLKSDTPKVESYEYKGGIKEYIAYMNRDK
QPLHEEIIYVQGERNNVQIEVSLQWCTDAYTDNVLGFANNIRTIDGGTHLEGLKAVLTRTLNTIARKRNKIKEHEPNLSG
EHVREGLTAVISVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGEVLTEYLEFHPGIADSILDKAIQAFKAAEAARHARELV
RRKSVLESSPLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRTQAILPLRGKILNIEKTDDSKIYKNNEIQALIT
ALGLGVKGEEFDSTQLRYHRIIIMTDADVDGAHIRTLILTFFYRYQRALIEQGFIYIACPPLYKVERGRNYEYCYSEREL
QQAIAKFPANANYTIQRFKGLGEMMPEQLWTTTMNPETRTLKRVEIEDAAEADRIFTILMGDRVAPRREFIETYGSKLNL
TDLDI
>Mature_644_residues
TSSYSADQIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEVVDNSIDEALAGYCTHIEVEINADGSVAVTDDGRGIPIDT
HSRTGKSALETVMTVLHAGGKFGGGGYKVSGGLHGVGISVVNALSEVVEVTVWRDKKVHTQRYERGVPVTELITNPYKEA
RTGTSVTFKPDTQIFTNSIEFDYITLSGRLRELAYLNAGVKITFTDNRLELLKSDTPKVESYEYKGGIKEYIAYMNRDKQ
PLHEEIIYVQGERNNVQIEVSLQWCTDAYTDNVLGFANNIRTIDGGTHLEGLKAVLTRTLNTIARKRNKIKEHEPNLSGE
HVREGLTAVISVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGEVLTEYLEFHPGIADSILDKAIQAFKAAEAARHARELVR
RKSVLESSPLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRTQAILPLRGKILNIEKTDDSKIYKNNEIQALITA
LGLGVKGEEFDSTQLRYHRIIIMTDADVDGAHIRTLILTFFYRYQRALIEQGFIYIACPPLYKVERGRNYEYCYSERELQ
QAIAKFPANANYTIQRFKGLGEMMPEQLWTTTMNPETRTLKRVEIEDAAEADRIFTILMGDRVAPRREFIETYGSKLNLT
DLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=614, Percent_Identity=25.0814332247557, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI19913408, Length=608, Percent_Identity=25, Blast_Score=139, Evalue=9e-33,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=53.2859680284192, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1789408, Length=643, Percent_Identity=39.50233281493, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=565, Percent_Identity=24.2477876106195, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=544, Percent_Identity=25, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=196, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=690, Percent_Identity=25.6521739130435, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17136538, Length=561, Percent_Identity=24.5989304812834, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72052; Mature: 71920

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSYSADQIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEVVDNSIDEALAGYCTHIEV
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHCCEEEEEE
EINADGSVAVTDDGRGIPIDTHSRTGKSALETVMTVLHAGGKFGGGGYKVSGGLHGVGIS
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEVVEVTVWRDKKVHTQRYERGVPVTELITNPYKEARTGTSVTFKPDTQIFTNSI
HHHHHHHHHHHHEECCCCHHHHHHHCCCCHHHHHCCCHHHHCCCCEEEECCCCCEEECCE
EFDYITLSGRLRELAYLNAGVKITFTDNRLELLKSDTPKVESYEYKGGIKEYIAYMNRDK
EEEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCC
QPLHEEIIYVQGERNNVQIEVSLQWCTDAYTDNVLGFANNIRTIDGGTHLEGLKAVLTRT
CCCCCCEEEEECCCCCEEEEEEEEECCCCHHCCHHHHCCCCEECCCCCCHHHHHHHHHHH
LNTIARKRNKIKEHEPNLSGEHVREGLTAVISVKVPDPEFEGQTKTKLGNTEVRGIVDSL
HHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHH
VGEVLTEYLEFHPGIADSILDKAIQAFKAAEAARHARELVRRKSVLESSPLPGKLADCSS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RDPSESEIYIVEGDSAGGSAKQGRDRRTQAILPLRGKILNIEKTDDSKIYKNNEIQALIT
CCCCCCEEEEEECCCCCCCCCCCCCCHHHEECCCCCEEEEEEECCCCCCCCCCHHHHHHH
ALGLGVKGEEFDSTQLRYHRIIIMTDADVDGAHIRTLILTFFYRYQRALIEQGFIYIACP
HHCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYKVERGRNYEYCYSERELQQAIAKFPANANYTIQRFKGLGEMMPEQLWTTTMNPETRT
CCHHHHCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
LKRVEIEDAAEADRIFTILMGDRVAPRREFIETYGSKLNLTDLDI
EEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEECEECCCC
>Mature Secondary Structure 
TSSYSADQIQVLEGLEAVRKRPGMYIGSTGPRGLHHLVYEVVDNSIDEALAGYCTHIEV
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHCCEEEEEE
EINADGSVAVTDDGRGIPIDTHSRTGKSALETVMTVLHAGGKFGGGGYKVSGGLHGVGIS
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEVVEVTVWRDKKVHTQRYERGVPVTELITNPYKEARTGTSVTFKPDTQIFTNSI
HHHHHHHHHHHHEECCCCHHHHHHHCCCCHHHHHCCCHHHHCCCCEEEECCCCCEEECCE
EFDYITLSGRLRELAYLNAGVKITFTDNRLELLKSDTPKVESYEYKGGIKEYIAYMNRDK
EEEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCC
QPLHEEIIYVQGERNNVQIEVSLQWCTDAYTDNVLGFANNIRTIDGGTHLEGLKAVLTRT
CCCCCCEEEEECCCCCEEEEEEEEECCCCHHCCHHHHCCCCEECCCCCCHHHHHHHHHHH
LNTIARKRNKIKEHEPNLSGEHVREGLTAVISVKVPDPEFEGQTKTKLGNTEVRGIVDSL
HHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHH
VGEVLTEYLEFHPGIADSILDKAIQAFKAAEAARHARELVRRKSVLESSPLPGKLADCSS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RDPSESEIYIVEGDSAGGSAKQGRDRRTQAILPLRGKILNIEKTDDSKIYKNNEIQALIT
CCCCCCEEEEEECCCCCCCCCCCCCCHHHEECCCCCEEEEEEECCCCCCCCCCHHHHHHH
ALGLGVKGEEFDSTQLRYHRIIIMTDADVDGAHIRTLILTFFYRYQRALIEQGFIYIACP
HHCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYKVERGRNYEYCYSERELQQAIAKFPANANYTIQRFKGLGEMMPEQLWTTTMNPETRT
CCHHHHCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
LKRVEIEDAAEADRIFTILMGDRVAPRREFIETYGSKLNLTDLDI
EEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEECEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]