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The map label for this gene is pmbA [H]

Identifier: 17232710

GI number: 17232710

Start: 6228174

End: 6229514

Strand: Reverse

Name: pmbA [H]

Synonym: all5218

Alternate gene names: 17232710

Gene position: 6229514-6228174 (Counterclockwise)

Preceding gene: 17232711

Following gene: 17232707

Centisome position: 97.13

GC content: 39.0

Gene sequence:

>1341_bases
ATGCCGAATATTAATGAAATCGCTACTTATGCCCAAGATAATGCTCAAAAGCTGGGCATCCAAAAATTTGACATTTATGG
CTCAACTGTAGATGAAACTAGTGTACAAGTTGACCAAGGTGAGCCAAAACAAGTCAAGGCTTCAAATCGCTCTGGTGTAA
CTGTACGCGTCTGGAATGAAGACAATACCATAGGTATTACTAGCACCACAGATGTAGATCCCAAAGGATTGGAATTAGCT
TTAAAAACGGCTTATGAAGCTAGTTTTTTTGGTGTGAAGGAAAATGTTCCTGATTTTAGTCCAGAGGCTACTGTTCCTCT
CGCTAATACGTTAAATGAGAAAGCCCCTCAAGCTCCTGTTTCGCAGCTCATAGAAAAATTGCTAGTAGCTGAAAAAGAAC
TGCTGTCAGCTCATCCGGCTATCAAAGGTGTACCTTATAACGGCTTGGCACAAAGAGATATTGATCGATTTTATCTCAAT
AGCCAAGGTGCTTTAAGAACTGAGTCTGTTTCTTTGTCATCAGTTTATCTGTACAGCAAAACTGAAGAGGAAGGGAAAAA
ACCACGCAGTGCAGGTGCTTACAGAATCAACCGTAGTTTAGAAAATTTGGATATCAATGGATGTATCCGAGAAACTGCTG
AGAAAACCATTAGTCATCTCAACTACGATAAAATTAAAACTGGTAAGTATCGAGTTGTTTTTTCACCGGAAGCTTTTTTA
AGTTTGCTAGGGGCTTTTTCTAATTTATTTAATGCCCAAAATATCCTTGATAAACAAAGCTTATCTACTCCTGATGATTT
AGGTAAGCAAATTGCTTCGCCTTTATTATCTGTTTATGATGATGCTCTACATCCTGCCAACATAGGTGCAGAAAGTTTTG
ATGGTGAAGGTACACCTACTCGCAAGGTGGCATTAATAGAAAATGGAATCTTAACAGGCTTTCTTCACAGTGCAGGGACT
GCAAAAAGGTTAAATACTCAACCGACAGGTAATGCTAGTATTGGGGCAAAAGTTAGTGTTAGTCCTAACTTTTATCATGT
GTTTAGCTCTGGGGATTCTGAGCAGAAACTTAACTTAGAAACAGCAGAAAATGTGATTTTGATCGATGATTTACACGCTC
TGCACGCTGGTGTGAAATCTTTGCAAGGATCTTTTTCTCTGCCCTTTGATGGTTGGTTGATTAACAAAGGTGTCAAAACA
AGTATTGAATCAGCAACAGTTGCTGGTGATTTCTTGGAACTTTTGAAATCAATTATTTACGTAGAGCCAGAAGTGGCTTT
AACACCTGGTGGTGTGGCTCCAAGAATTTGGGTAGAGGAATTATCAATCACTGGCGAATAA

Upstream 100 bases:

>100_bases
CTACGTATATTTTTTAGGCGAAAAACTTACATCATAATATCCAAAAATCCAAAGTTTAATCTACTTATCAAAATACAAAA
TCTAAAATCAAAAATTCACT

Downstream 100 bases:

>100_bases
AAGTTAGAAGTGATTTTGAGTTAATTAGTGGGGTATTTTCAACCCCACTAATTTTTTTTACAATGGCGCGTTAGGAGAAC
ACCAAAAAATAAATTATCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNEDNTIGITSTTDVDPKGLELA
LKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPVSQLIEKLLVAEKELLSAHPAIKGVPYNGLAQRDIDRFYLN
SQGALRTESVSLSSVYLYSKTEEEGKKPRSAGAYRINRSLENLDINGCIRETAEKTISHLNYDKIKTGKYRVVFSPEAFL
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPTRKVALIENGILTGFLHSAGT
AKRLNTQPTGNASIGAKVSVSPNFYHVFSSGDSEQKLNLETAENVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKT
SIESATVAGDFLELLKSIIYVEPEVALTPGGVAPRIWVEELSITGE

Sequences:

>Translated_446_residues
MPNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNEDNTIGITSTTDVDPKGLELA
LKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPVSQLIEKLLVAEKELLSAHPAIKGVPYNGLAQRDIDRFYLN
SQGALRTESVSLSSVYLYSKTEEEGKKPRSAGAYRINRSLENLDINGCIRETAEKTISHLNYDKIKTGKYRVVFSPEAFL
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPTRKVALIENGILTGFLHSAGT
AKRLNTQPTGNASIGAKVSVSPNFYHVFSSGDSEQKLNLETAENVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKT
SIESATVAGDFLELLKSIIYVEPEVALTPGGVAPRIWVEELSITGE
>Mature_445_residues
PNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNEDNTIGITSTTDVDPKGLELAL
KTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPVSQLIEKLLVAEKELLSAHPAIKGVPYNGLAQRDIDRFYLNS
QGALRTESVSLSSVYLYSKTEEEGKKPRSAGAYRINRSLENLDINGCIRETAEKTISHLNYDKIKTGKYRVVFSPEAFLS
LLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPTRKVALIENGILTGFLHSAGTA
KRLNTQPTGNASIGAKVSVSPNFYHVFSSGDSEQKLNLETAENVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKTS
IESATVAGDFLELLKSIIYVEPEVALTPGGVAPRIWVEELSITGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=423, Percent_Identity=23.4042553191489, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48273; Mature: 48141

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNE
CCCHHHHHHHHHCCHHHCCCEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCEEEEECC
DNTIGITSTTDVDPKGLELALKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPV
CCEEEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHH
SQLIEKLLVAEKELLSAHPAIKGVPYNGLAQRDIDRFYLNSQGALRTESVSLSSVYLYSK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHEECCCCCEEECCEEEEEEEEEEC
TEEEGKKPRSAGAYRINRSLENLDINGCIRETAEKTISHLNYDKIKTGKYRVVFSPEAFL
CHHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHH
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RKVALIENGILTGFLHSAGTAKRLNTQPTGNASIGAKVSVSPNFYHVFSSGDSEQKLNLE
EEEEEEECCCHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEEE
TAENVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKTSIESATVAGDFLELLKSIIY
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHE
VEPEVALTPGGVAPRIWVEELSITGE
ECCCEEECCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
PNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNE
CCHHHHHHHHHCCHHHCCCEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCEEEEECC
DNTIGITSTTDVDPKGLELALKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPV
CCEEEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHH
SQLIEKLLVAEKELLSAHPAIKGVPYNGLAQRDIDRFYLNSQGALRTESVSLSSVYLYSK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHEECCCCCEEECCEEEEEEEEEEC
TEEEGKKPRSAGAYRINRSLENLDINGCIRETAEKTISHLNYDKIKTGKYRVVFSPEAFL
CHHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHH
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RKVALIENGILTGFLHSAGTAKRLNTQPTGNASIGAKVSVSPNFYHVFSSGDSEQKLNLE
EEEEEEECCCHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEEE
TAENVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKTSIESATVAGDFLELLKSIIY
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHE
VEPEVALTPGGVAPRIWVEELSITGE
ECCCEEECCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]