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The map label for this gene is ndhB [H]

Identifier: 17232375

GI number: 17232375

Start: 5818141

End: 5819703

Strand: Reverse

Name: ndhB [H]

Synonym: all4883

Alternate gene names: 17232375

Gene position: 5819703-5818141 (Counterclockwise)

Preceding gene: 17232376

Following gene: 17232367

Centisome position: 90.74

GC content: 45.68

Gene sequence:

>1563_bases
ATGGATTTTGCTAATCTTGCAGCCCAGTTAAATGCTGGAACAATTTTGCCAGAGGGAATTGTAATTGTCACCCTCATGGG
GGTTTTGATTGTTGATTTGATTTTAGGGCGTACATCCTCACGCTGGATAGGATATCTGGCGATCGCAGGCTTACTCGCGG
CGATCGTCGCCCTGTATTTTCAATGGGATGCTACCAATCCCATCTCTTTTACTGGCGCGTTTATCGGTGATGACCTCAGC
ATCATCTTTCGCGGTATCATCGCCCTGTCTGCCGTTGTTACCATCTTGATGTCAATTCGCTATGTTGAGCAGAGTGGAAC
TGCTTTAGCGGAATTCATCGCTATTTTGTTAACTGCGACTTTGGGCGGGATGTTTGTCTCTGGGGCTAGTGAGTTGGTGA
TGATCTTCATCTCCCTAGAAACCTTAAGTATCTCCTCGTATTTGTTAACAGGTTATACCAAGCGTGACCCCCGCTCTAAT
GAAGCGGCGCTGAAATACTTGTTAATTGGCGCTTCCAGCACAGCAGTATTCTTGTATGGCGTTTCACTGCTATACGGTCT
ATCTGGTGGACAAACAGAACTGAATGCGATCGCTAATGGAATTATTACAGCTAACGTTGGTCAATCACTAGGTGCAGTTA
TTGCCCTGGTTTTTGTCATAGCGGGTATTGGCTTTAAGATTTCCGCCGCACCTTTCCACCAATGGACACCAGACGTATAT
GAAGGCGCTCCCACCCCAGTGATTGCCTTTTTATCCGTTGGTTCTAAGGCAGCAGGCTTTGCCCTCGCTATCCGTCTGTT
AACTACAGTTTTCCCCTTCGTCGCCGAAGAGTGGAAGTTTGTGTTCACAGCTTTGGCCGTCCTCAGCATGATTTTGGGTA
ACGTTGTCGCCCTAGCCCAAACCAGCATGAAACGGATGCTGGCTTATTCATCCATTGCCCAAGCCGGGTTTGTGATGATT
GGCTTGATTGCTGGAACTGATGCCGGATATGCCAGCATGATCTTTTACTTGCTGGTTTACCTATTCATGAATCTGTGCGG
TTTTACCTGCATCATTCTGTTCTCCCTGCGGACAGGAACCGACCAAATTGCCGAATACTCTGGGTTATATCAAAAAGACC
CACTCCTCACACTAGGTTTGAGTATCTCCCTGCTTTCATTGGGCGGTATTCCTCCACTAGCTGGGTTTTTTGGCAAAATT
TACTTATTCTGGGCTGGTTGGCAAGCAGGTCTTTACTGGTTGGTCTTACTAGGCTTAGTAACCAGTGTTATCTCCATCTA
CTACTACATTCGCGTAGTCAAAATGATGGTGGTCAAAGAACCCCAAGAAATGTCCGATGTAGTCAAAAATTACCCCGAAA
TACGTTGGAATTTACCTGGATTTAGACCATTGCAAGTGGGTTTAGTGTTGACTTTGATTGCTACCTCTGTAGCAGGAATT
TTATCTAATCCACTCTTTACTTTGGCAAATAATTCTGTTGCCAATACTGCAATTCTGCAAGCCACAAAAGTTGTCAGTAC
TCAGGTAAGTGCTATTCCTGCTGAGAAACCAGACGGTTTGTAA

Upstream 100 bases:

>100_bases
AAACTAAAAGCTAAAAAGATAAATGCAACTTTGGTCAGGAACAATAGATAGAATGAACAAAGTTAGCCTTGATGTCTAAT
CGCCAAAACCTATATATCTC

Downstream 100 bases:

>100_bases
GCTCTATCTCAAATCTATACTATCCCCGATTTTTCCCAAAATTCGGGGATATTTTTTTAACCTATTCAATAGGAAGAGTA
ATAATAAATTCCGAACCCTC

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2 [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MDFANLAAQLNAGTILPEGIVIVTLMGVLIVDLILGRTSSRWIGYLAIAGLLAAIVALYFQWDATNPISFTGAFIGDDLS
IIFRGIIALSAVVTILMSIRYVEQSGTALAEFIAILLTATLGGMFVSGASELVMIFISLETLSISSYLLTGYTKRDPRSN
EAALKYLLIGASSTAVFLYGVSLLYGLSGGQTELNAIANGIITANVGQSLGAVIALVFVIAGIGFKISAAPFHQWTPDVY
EGAPTPVIAFLSVGSKAAGFALAIRLLTTVFPFVAEEWKFVFTALAVLSMILGNVVALAQTSMKRMLAYSSIAQAGFVMI
GLIAGTDAGYASMIFYLLVYLFMNLCGFTCIILFSLRTGTDQIAEYSGLYQKDPLLTLGLSISLLSLGGIPPLAGFFGKI
YLFWAGWQAGLYWLVLLGLVTSVISIYYYIRVVKMMVVKEPQEMSDVVKNYPEIRWNLPGFRPLQVGLVLTLIATSVAGI
LSNPLFTLANNSVANTAILQATKVVSTQVSAIPAEKPDGL

Sequences:

>Translated_520_residues
MDFANLAAQLNAGTILPEGIVIVTLMGVLIVDLILGRTSSRWIGYLAIAGLLAAIVALYFQWDATNPISFTGAFIGDDLS
IIFRGIIALSAVVTILMSIRYVEQSGTALAEFIAILLTATLGGMFVSGASELVMIFISLETLSISSYLLTGYTKRDPRSN
EAALKYLLIGASSTAVFLYGVSLLYGLSGGQTELNAIANGIITANVGQSLGAVIALVFVIAGIGFKISAAPFHQWTPDVY
EGAPTPVIAFLSVGSKAAGFALAIRLLTTVFPFVAEEWKFVFTALAVLSMILGNVVALAQTSMKRMLAYSSIAQAGFVMI
GLIAGTDAGYASMIFYLLVYLFMNLCGFTCIILFSLRTGTDQIAEYSGLYQKDPLLTLGLSISLLSLGGIPPLAGFFGKI
YLFWAGWQAGLYWLVLLGLVTSVISIYYYIRVVKMMVVKEPQEMSDVVKNYPEIRWNLPGFRPLQVGLVLTLIATSVAGI
LSNPLFTLANNSVANTAILQATKVVSTQVSAIPAEKPDGL
>Mature_520_residues
MDFANLAAQLNAGTILPEGIVIVTLMGVLIVDLILGRTSSRWIGYLAIAGLLAAIVALYFQWDATNPISFTGAFIGDDLS
IIFRGIIALSAVVTILMSIRYVEQSGTALAEFIAILLTATLGGMFVSGASELVMIFISLETLSISSYLLTGYTKRDPRSN
EAALKYLLIGASSTAVFLYGVSLLYGLSGGQTELNAIANGIITANVGQSLGAVIALVFVIAGIGFKISAAPFHQWTPDVY
EGAPTPVIAFLSVGSKAAGFALAIRLLTTVFPFVAEEWKFVFTALAVLSMILGNVVALAQTSMKRMLAYSSIAQAGFVMI
GLIAGTDAGYASMIFYLLVYLFMNLCGFTCIILFSLRTGTDQIAEYSGLYQKDPLLTLGLSISLLSLGGIPPLAGFFGKI
YLFWAGWQAGLYWLVLLGLVTSVISIYYYIRVVKMMVVKEPQEMSDVVKNYPEIRWNLPGFRPLQVGLVLTLIATSVAGI
LSNPLFTLANNSVANTAILQATKVVSTQVSAIPAEKPDGL

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=406, Percent_Identity=23.1527093596059, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI145693160, Length=446, Percent_Identity=34.5291479820628, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1788829, Length=463, Percent_Identity=26.133909287257, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788831, Length=346, Percent_Identity=24.8554913294798, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788614, Length=323, Percent_Identity=25.077399380805, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788613, Length=355, Percent_Identity=25.6338028169014, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788827, Length=327, Percent_Identity=26.2996941896024, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 55465; Mature: 55465

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFANLAAQLNAGTILPEGIVIVTLMGVLIVDLILGRTSSRWIGYLAIAGLLAAIVALYF
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QWDATNPISFTGAFIGDDLSIIFRGIIALSAVVTILMSIRYVEQSGTALAEFIAILLTAT
HCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LGGMFVSGASELVMIFISLETLSISSYLLTGYTKRDPRSNEAALKYLLIGASSTAVFLYG
HHHHHHCCHHHHEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCHHHHHHH
VSLLYGLSGGQTELNAIANGIITANVGQSLGAVIALVFVIAGIGFKISAAPFHQWTPDVY
HHHHHCCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHH
EGAPTPVIAFLSVGSKAAGFALAIRLLTTVFPFVAEEWKFVFTALAVLSMILGNVVALAQ
CCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSMKRMLAYSSIAQAGFVMIGLIAGTDAGYASMIFYLLVYLFMNLCGFTCIILFSLRTGT
HHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DQIAEYSGLYQKDPLLTLGLSISLLSLGGIPPLAGFFGKIYLFWAGWQAGLYWLVLLGLV
HHHHHHCCCCCCCCCEEECHHHHHHHCCCCCHHHHHHHHHHHEEECHHHHHHHHHHHHHH
TSVISIYYYIRVVKMMVVKEPQEMSDVVKNYPEIRWNLPGFRPLQVGLVLTLIATSVAGI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
LSNPLFTLANNSVANTAILQATKVVSTQVSAIPAEKPDGL
HCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDFANLAAQLNAGTILPEGIVIVTLMGVLIVDLILGRTSSRWIGYLAIAGLLAAIVALYF
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QWDATNPISFTGAFIGDDLSIIFRGIIALSAVVTILMSIRYVEQSGTALAEFIAILLTAT
HCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LGGMFVSGASELVMIFISLETLSISSYLLTGYTKRDPRSNEAALKYLLIGASSTAVFLYG
HHHHHHCCHHHHEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCHHHHHHH
VSLLYGLSGGQTELNAIANGIITANVGQSLGAVIALVFVIAGIGFKISAAPFHQWTPDVY
HHHHHCCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHH
EGAPTPVIAFLSVGSKAAGFALAIRLLTTVFPFVAEEWKFVFTALAVLSMILGNVVALAQ
CCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSMKRMLAYSSIAQAGFVMIGLIAGTDAGYASMIFYLLVYLFMNLCGFTCIILFSLRTGT
HHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DQIAEYSGLYQKDPLLTLGLSISLLSLGGIPPLAGFFGKIYLFWAGWQAGLYWLVLLGLV
HHHHHHCCCCCCCCCEEECHHHHHHHCCCCCHHHHHHHHHHHEEECHHHHHHHHHHHHHH
TSVISIYYYIRVVKMMVVKEPQEMSDVVKNYPEIRWNLPGFRPLQVGLVLTLIATSVAGI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
LSNPLFTLANNSVANTAILQATKVVSTQVSAIPAEKPDGL
HCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA