Click here to switch to the map view.

The map label for this gene is cbiF [H]

Identifier: 17232190

GI number: 17232190

Start: 5602904

End: 5603689

Strand: Reverse

Name: cbiF [H]

Synonym: all4698

Alternate gene names: 17232190

Gene position: 5603689-5602904 (Counterclockwise)

Preceding gene: 17232191

Following gene: 17232189

Centisome position: 87.37

GC content: 44.78

Gene sequence:

>786_bases
ATGCAAAATCATGTAAATTCCCTAGCTTCCGCCGTGTATATTGTAGGCGCAGGGCCTGGAGACCCAGATTTACTGACAAT
TAAAGCCCAAAAATTACTGTCAGTAGCAGATGTAATTTTATTCGCAGATTCGTTAATACCAGAAGAGCTTTTGCAGTTTT
GCCGCAAAGATGCAGAAATTATTCAGACTGCGAACAAAACTTTGGAGGAGATTATACCTTTGATGATCGCAAGGGTGCGA
TCGCAAAAATCTGTAGTACGTCTCCACTCAGGTGATCCCAGTCTCTACAGCGCAATTAATGAGCAAATGCAGCTCCTGGC
GGCAGCTAATATTCCTTTTGAAGTTATTCCTGGTATTAGTGTTTTCCAAGCCGCAGCCGCCAAACTGAAGGTAGAATTGA
CTGTTCCTGGTTTAGTCCAGACAATCATTCTGACGCGCATTAGTGGACGAACACAAGTCCCACATACAGAAGAATTAGCC
AGCCTCGCAGCACATCAAGCTAGTTTATGCTTATATCTGAGTGCGCGCCATGTAGAAAATGCTCAAGCTAAACTACTAGA
ACACTATCCCCCAGATACACCTGTAGCTATTTGCTTTCGCATAGGATGGCCAGATGAAAAAATTCGTGTAGTTCCTCTCC
AGGAGATGGCAGACTGTACTCAGCGAGAAAATCTGATTCGCACCACGTTATATGTGATTAGTCCTGCATTATCACACACC
ACCGGGCGATCGCGCTTATATCATCCTGAGCATAGTCATCTGTTCCGCACGGCTGGAGATAGCTGA

Upstream 100 bases:

>100_bases
CTAGAGGGATAATCCGGAAAGATACCGAGCAAATTCTCAAAAAATTTTTTGATTTTTTTATGTCTACTGATAATTACTTA
GATTTTATATAGAACTTTGT

Downstream 100 bases:

>100_bases
TAATTCAGAATAATTTTTTATCTGCAAGTCCCTAAAAACTATGAGTTCTCAAAAGGAAATATTCAAGCCTTGACTAACCG
ATTTTCATTAAAAGAAAAAC

Product: precorrin-3 methylase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MQNHVNSLASAVYIVGAGPGDPDLLTIKAQKLLSVADVILFADSLIPEELLQFCRKDAEIIQTANKTLEEIIPLMIARVR
SQKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGISVFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPHTEELA
SLAAHQASLCLYLSARHVENAQAKLLEHYPPDTPVAICFRIGWPDEKIRVVPLQEMADCTQRENLIRTTLYVISPALSHT
TGRSRLYHPEHSHLFRTAGDS

Sequences:

>Translated_261_residues
MQNHVNSLASAVYIVGAGPGDPDLLTIKAQKLLSVADVILFADSLIPEELLQFCRKDAEIIQTANKTLEEIIPLMIARVR
SQKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGISVFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPHTEELA
SLAAHQASLCLYLSARHVENAQAKLLEHYPPDTPVAICFRIGWPDEKIRVVPLQEMADCTQRENLIRTTLYVISPALSHT
TGRSRLYHPEHSHLFRTAGDS
>Mature_261_residues
MQNHVNSLASAVYIVGAGPGDPDLLTIKAQKLLSVADVILFADSLIPEELLQFCRKDAEIIQTANKTLEEIIPLMIARVR
SQKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGISVFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPHTEELA
SLAAHQASLCLYLSARHVENAQAKLLEHYPPDTPVAICFRIGWPDEKIRVVPLQEMADCTQRENLIRTTLYVISPALSHT
TGRSRLYHPEHSHLFRTAGDS

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=193, Percent_Identity=32.6424870466321, Blast_Score=107, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322922, Length=208, Percent_Identity=25.9615384615385, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28692; Mature: 28692

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNHVNSLASAVYIVGAGPGDPDLLTIKAQKLLSVADVILFADSLIPEELLQFCRKDAEI
CCHHHHHHHHEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IQTANKTLEEIIPLMIARVRSQKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGIS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHCCCHH
VFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPHTEELASLAAHQASLCLYLSARHVEN
HHHHHHHHEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEHHHHHHH
AQAKLLEHYPPDTPVAICFRIGWPDEKIRVVPLQEMADCTQRENLIRTTLYVISPALSHT
HHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGRSRLYHPEHSHLFRTAGDS
CCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MQNHVNSLASAVYIVGAGPGDPDLLTIKAQKLLSVADVILFADSLIPEELLQFCRKDAEI
CCHHHHHHHHEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IQTANKTLEEIIPLMIARVRSQKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGIS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHCCCHH
VFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPHTEELASLAAHQASLCLYLSARHVEN
HHHHHHHHEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEHHHHHHH
AQAKLLEHYPPDTPVAICFRIGWPDEKIRVVPLQEMADCTQRENLIRTTLYVISPALSHT
HHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGRSRLYHPEHSHLFRTAGDS
CCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]