BacMap

Definition	Nostoc sp. PCC 7120, complete genome.
Accession	NC_003272
Length	6,413,771

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The map label for this gene is rfbB [H]

Identifier: 17231978

GI number: 17231978

Start: 5370206

End: 5370946

Strand: Direct

Name: rfbB [H]

Synonym: alr4486

Alternate gene names: 17231978

Gene position: 5370206-5370946 (Clockwise)

Preceding gene: 17231977

Following gene: 17231979

Centisome position: 83.73

GC content: 36.71

Gene sequence:

>741_bases
ATGGAAGTAATTCGTCTGGATAAAGTTTCGCTATGGCGGCGAACCCAAGAAGAGTTTTCTTATGACCTCAAGAAAACTCT
CCTATCTTTGATAGAAGGTAAATATCGTAAGCCAGCGAAAAAATTGGTTTTAGACAACGTCGATTTAGTTGTTGAAAAAG
GCGACAAAATTGGTATTATTGGCGCAAATGGATCTGGTAAATCAACTTTATTGAAGATCATTTCTGGTATACTTCAACCA
ACGACTGGCACAGTCAGAGTCAAAGGGCAAGTTGCACCTTTGATTGAACTAGGTGCTGGTTTTGATGCAGAAATATCTGT
AATGGATAATATTCTGCTCTATGGTGTATTACTGGGATTTTCGAGGGCGGAAATGAAAGAAAGGGCGAACTCAATATTGG
AGTTTGCAGAATTACAGGACTATGCTTTAGTTCCTGTAAAAGGACTTTCTTCTGGTATGGTTGCTCGTTTAGGGTTTTCC
ATTGCCACAGATGTGCAGCCAAATATCTTAATTTTAGATGAGGTCTTATCGGTAGGAGACGAAAATTTTAAAAATAAATG
CAAGCAAAGAATTGATAATTTTTGGAATGGAGATGCAACAGTTTTAGTAGTTTCCCATGATTTACAGTTTGTTAAACAAT
ATTGTAATCAGTTGGTTTGGTTATCTCATGGAAAGATAGAGTTTATAGGCGATTCAGGAGAGGCAATTCAACATTATTTA
AGTACATTAACATCCACATGA

Upstream 100 bases:

>100_bases
AAGTTTAATTTGGGGTGGTTTACTCGGCGGCATAATTATTTTGTCATTGGGATGGTTTTGTTTTCAGTTGTGGCGACATC
AATTTATGGATCTGCTGTAG

Downstream 100 bases:

>100_bases
ATATCTAAACATGGATACTTTCAATTATCAAAATTTTTATAATCAAGAATATTATCTAAATATGGTACCGGGTATTCCTT
ATGATAGGGAAGCTCATGGT

Product: ABC transporter ATP binding subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MEVIRLDKVSLWRRTQEEFSYDLKKTLLSLIEGKYRKPAKKLVLDNVDLVVEKGDKIGIIGANGSGKSTLLKIISGILQP
TTGTVRVKGQVAPLIELGAGFDAEISVMDNILLYGVLLGFSRAEMKERANSILEFAELQDYALVPVKGLSSGMVARLGFS
IATDVQPNILILDEVLSVGDENFKNKCKQRIDNFWNGDATVLVVSHDLQFVKQYCNQLVWLSHGKIEFIGDSGEAIQHYL
STLTST

Sequences:

>Translated_246_residues
MEVIRLDKVSLWRRTQEEFSYDLKKTLLSLIEGKYRKPAKKLVLDNVDLVVEKGDKIGIIGANGSGKSTLLKIISGILQP
TTGTVRVKGQVAPLIELGAGFDAEISVMDNILLYGVLLGFSRAEMKERANSILEFAELQDYALVPVKGLSSGMVARLGFS
IATDVQPNILILDEVLSVGDENFKNKCKQRIDNFWNGDATVLVVSHDLQFVKQYCNQLVWLSHGKIEFIGDSGEAIQHYL
STLTST
>Mature_246_residues
MEVIRLDKVSLWRRTQEEFSYDLKKTLLSLIEGKYRKPAKKLVLDNVDLVVEKGDKIGIIGANGSGKSTLLKIISGILQP
TTGTVRVKGQVAPLIELGAGFDAEISVMDNILLYGVLLGFSRAEMKERANSILEFAELQDYALVPVKGLSSGMVARLGFS
IATDVQPNILILDEVLSVGDENFKNKCKQRIDNFWNGDATVLVVSHDLQFVKQYCNQLVWLSHGKIEFIGDSGEAIQHYL
STLTST

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=212, Percent_Identity=29.2452830188679, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI27436953, Length=210, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI6005701, Length=224, Percent_Identity=27.6785714285714, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI153792144, Length=232, Percent_Identity=26.7241379310345, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI27262624, Length=231, Percent_Identity=25.5411255411255, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27262626, Length=231, Percent_Identity=25.5411255411255, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI30795238, Length=211, Percent_Identity=22.7488151658768, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27881501, Length=211, Percent_Identity=22.7488151658768, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI31657092, Length=216, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI47078218, Length=216, Percent_Identity=26.8518518518519, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI45446740, Length=216, Percent_Identity=26.8518518518519, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI148612853, Length=210, Percent_Identity=26.1904761904762, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI66529005, Length=208, Percent_Identity=27.8846153846154, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87081791, Length=206, Percent_Identity=26.6990291262136, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081709, Length=201, Percent_Identity=26.865671641791, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787182, Length=198, Percent_Identity=34.3434343434343, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789891, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789032, Length=211, Percent_Identity=25.1184834123223, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788761, Length=202, Percent_Identity=27.7227722772277, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787029, Length=200, Percent_Identity=26.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788225, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI48994943, Length=207, Percent_Identity=24.6376811594203, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786563, Length=196, Percent_Identity=27.5510204081633, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786703, Length=205, Percent_Identity=28.780487804878, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786398, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789873, Length=203, Percent_Identity=26.1083743842365, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081782, Length=204, Percent_Identity=24.0196078431373, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789751, Length=204, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787041, Length=237, Percent_Identity=27.4261603375527, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081834, Length=197, Percent_Identity=30.4568527918782, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788165, Length=188, Percent_Identity=27.6595744680851, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48995001, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788450, Length=205, Percent_Identity=27.3170731707317, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787164, Length=210, Percent_Identity=29.0476190476191, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786319, Length=210, Percent_Identity=26.1904761904762, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1786872, Length=181, Percent_Identity=28.1767955801105, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367384, Length=193, Percent_Identity=27.979274611399, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1790467, Length=209, Percent_Identity=24.8803827751196, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=206, Percent_Identity=24.7572815533981, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=232, Percent_Identity=24.5689655172414, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321121, Length=183, Percent_Identity=28.9617486338798, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574150, Length=221, Percent_Identity=29.8642533936652, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI116007328, Length=221, Percent_Identity=29.8642533936652, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI116007184, Length=181, Percent_Identity=24.8618784530387, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221500365, Length=181, Percent_Identity=24.8618784530387, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574259, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362751, Length=191, Percent_Identity=28.7958115183246, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62484234, Length=198, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650853, Length=198, Percent_Identity=29.2929292929293, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24650855, Length=198, Percent_Identity=29.2929292929293, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585091, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648279, Length=229, Percent_Identity=27.0742358078603, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550390, Length=218, Percent_Identity=27.5229357798165, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161078426, Length=229, Percent_Identity=27.0742358078603, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585557, Length=215, Percent_Identity=28.8372093023256, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552437, Length=215, Percent_Identity=28.8372093023256, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI85725262, Length=187, Percent_Identity=25.668449197861, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27189; Mature: 27189

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIRLDKVSLWRRTQEEFSYDLKKTLLSLIEGKYRKPAKKLVLDNVDLVVEKGDKIGII
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCEEEEE
GANGSGKSTLLKIISGILQPTTGTVRVKGQVAPLIELGAGFDAEISVMDNILLYGVLLGF
ECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCC
SRAEMKERANSILEFAELQDYALVPVKGLSSGMVARLGFSIATDVQPNILILDEVLSVGD
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCHHCCCCCCCEEEHHHHHHCCC
ENFKNKCKQRIDNFWNGDATVLVVSHDLQFVKQYCNQLVWLSHGKIEFIGDSGEAIQHYL
HHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHEEECCCEEEEECCCHHHHHHHH
STLTST
HHHHCC
>Mature Secondary Structure
MEVIRLDKVSLWRRTQEEFSYDLKKTLLSLIEGKYRKPAKKLVLDNVDLVVEKGDKIGII
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCEEEEE
GANGSGKSTLLKIISGILQPTTGTVRVKGQVAPLIELGAGFDAEISVMDNILLYGVLLGF
ECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCC
SRAEMKERANSILEFAELQDYALVPVKGLSSGMVARLGFSIATDVQPNILILDEVLSVGD
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCHHCCCCCCCEEEHHHHHHCCC
ENFKNKCKQRIDNFWNGDATVLVVSHDLQFVKQYCNQLVWLSHGKIEFIGDSGEAIQHYL
HHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHEEECCCEEEEECCCHHHHHHHH
STLTST
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8626291 [H]