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The map label for this gene is tobE [H]

Identifier: 17231950

GI number: 17231950

Start: 5342428

End: 5343384

Strand: Reverse

Name: tobE [H]

Synonym: all4458

Alternate gene names: 17231950

Gene position: 5343384-5342428 (Counterclockwise)

Preceding gene: 17231951

Following gene: 17231945

Centisome position: 83.31

GC content: 45.66

Gene sequence:

>957_bases
ATGAAAGGACTCTGGCTAGAAAATAACCAGTTACAATTGCTTACAGATATTCCCGTTCCGGAAGCACCACCGGGAGAAGC
GCTTGTACGGGTTCTGCGTGCGGGAATTTGCAACACAGATTTGGAGTTGCTGCGGGGCTATTATCCCTACACAGGAATTT
TAGGACACGAATTTGTGGGACTGGTGGAACAAGGGCCAGAACATCTACTAAATCAAAGAGTCGTCGGGGAAATCAACGCT
GTCTGCGGTTATTGTCGTTTTTGTCGCCGAGGACAACCAACTCACTGTGAAAATCGGACAGTTTTAGGTATTGTCAACCG
TCATGGAGCATTTGCAGAGTACCTTTGTTTACCAGTAGAAAATCTCCATGTTGTACCTGAAAATGTACCAACAGACGCGG
CAACTTTTACCGAACCTTTAGCAGCCGCCCTAGAAATTCAACAACAGATAATATTGAGTAGAGATGACCGTGTGCTAGTA
GTCGGAGATGGCAAATTGGGACAATTAGTAGCCCAGACATTAGCTCTAACTGGTTGTGAACTATTGGTTGTTGGTCGTCA
CCAAGAAAAACTTGCTAATTTAAAAGTACGGGGTGTGCAAACGGGTTTAGTTGATGCTGTCCAAGATAGAGCTTTTGATA
TTGTAGTCGAGTGTACTGGTAATCCAGAAGGATTTGCGATCGCTCGTCGGGCTTTGCGTCCCCGTGGTACTCTAGTGCTG
AAAAGCACCTATGCTGGCAATCTCAGCTTAGATGCTTCTTCTTTAGTAGTAGACGAAATTACTCTCATCGGTTCTCGTTG
TGGTGTTTTTCCCCCTGCACTGGAGCTATTAGCTACAGGAAAAGTTGACGTAGAACCCCTGATTCATAGCCAATACCCCC
TCACTCAAGGACTTTTGGCCTTTGAGGAAGCACAACGCCGAGGCGTGTTGAAAGTTTTGCTGGAAATGGGTAATTAG

Upstream 100 bases:

>100_bases
TCAAAACTTTAGGAAGTGAGATTGCTGTTCACTCTGATGACTTAGTAATTTGGTTAAGCAAGATTGAGCAGAAGTTTCCC
AATACTACTGAGATATAAAG

Downstream 100 bases:

>100_bases
TAAACCGTTAACCTAAACTAATACAAATAAGGATTAGTCTTCGCTTGATAATCCATACAGTTGATGGCTTCTTCTGTGTT
AGCACGGGATGGGTGTACAG

Product: hypothetical protein

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKGLWLENNQLQLLTDIPVPEAPPGEALVRVLRAGICNTDLELLRGYYPYTGILGHEFVGLVEQGPEHLLNQRVVGEINA
VCGYCRFCRRGQPTHCENRTVLGIVNRHGAFAEYLCLPVENLHVVPENVPTDAATFTEPLAAALEIQQQIILSRDDRVLV
VGDGKLGQLVAQTLALTGCELLVVGRHQEKLANLKVRGVQTGLVDAVQDRAFDIVVECTGNPEGFAIARRALRPRGTLVL
KSTYAGNLSLDASSLVVDEITLIGSRCGVFPPALELLATGKVDVEPLIHSQYPLTQGLLAFEEAQRRGVLKVLLEMGN

Sequences:

>Translated_318_residues
MKGLWLENNQLQLLTDIPVPEAPPGEALVRVLRAGICNTDLELLRGYYPYTGILGHEFVGLVEQGPEHLLNQRVVGEINA
VCGYCRFCRRGQPTHCENRTVLGIVNRHGAFAEYLCLPVENLHVVPENVPTDAATFTEPLAAALEIQQQIILSRDDRVLV
VGDGKLGQLVAQTLALTGCELLVVGRHQEKLANLKVRGVQTGLVDAVQDRAFDIVVECTGNPEGFAIARRALRPRGTLVL
KSTYAGNLSLDASSLVVDEITLIGSRCGVFPPALELLATGKVDVEPLIHSQYPLTQGLLAFEEAQRRGVLKVLLEMGN
>Mature_318_residues
MKGLWLENNQLQLLTDIPVPEAPPGEALVRVLRAGICNTDLELLRGYYPYTGILGHEFVGLVEQGPEHLLNQRVVGEINA
VCGYCRFCRRGQPTHCENRTVLGIVNRHGAFAEYLCLPVENLHVVPENVPTDAATFTEPLAAALEIQQQIILSRDDRVLV
VGDGKLGQLVAQTLALTGCELLVVGRHQEKLANLKVRGVQTGLVDAVQDRAFDIVVECTGNPEGFAIARRALRPRGTLVL
KSTYAGNLSLDASSLVVDEITLIGSRCGVFPPALELLATGKVDVEPLIHSQYPLTQGLLAFEEAQRRGVLKVLLEMGN

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=334, Percent_Identity=25.4491017964072, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787863, Length=340, Percent_Identity=28.8235294117647, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1790045, Length=340, Percent_Identity=25.2941176470588, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788075, Length=337, Percent_Identity=26.7062314540059, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI226510992, Length=303, Percent_Identity=27.0627062706271, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790718, Length=302, Percent_Identity=25.1655629139073, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788407, Length=294, Percent_Identity=26.1904761904762, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788073, Length=333, Percent_Identity=23.1231231231231, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082125, Length=269, Percent_Identity=26.0223048327138, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786825, Length=224, Percent_Identity=27.6785714285714, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=333, Percent_Identity=25.8258258258258, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562878, Length=329, Percent_Identity=24.0121580547112, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323729, Length=294, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=291, Percent_Identity=25.7731958762887, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=344, Percent_Identity=23.2558139534884, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=290, Percent_Identity=24.4827586206897, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=338, Percent_Identity=22.189349112426, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17737897, Length=330, Percent_Identity=25.1515151515152, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17137530, Length=328, Percent_Identity=23.4756097560976, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 34607; Mature: 34607

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLWLENNQLQLLTDIPVPEAPPGEALVRVLRAGICNTDLELLRGYYPYTGILGHEFVG
CCCCEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHH
LVEQGPEHLLNQRVVGEINAVCGYCRFCRRGQPTHCENRTVLGIVNRHGAFAEYLCLPVE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHEECCC
NLHVVPENVPTDAATFTEPLAAALEIQQQIILSRDDRVLVVGDGKLGQLVAQTLALTGCE
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCE
LLVVGRHQEKLANLKVRGVQTGLVDAVQDRAFDIVVECTGNPEGFAIARRALRPRGTLVL
EEEECCCHHHHHCEEEECCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEE
KSTYAGNLSLDASSLVVDEITLIGSRCGVFPPALELLATGKVDVEPLIHSQYPLTQGLLA
EEECCCCEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHH
FEEAQRRGVLKVLLEMGN
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGLWLENNQLQLLTDIPVPEAPPGEALVRVLRAGICNTDLELLRGYYPYTGILGHEFVG
CCCCEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHH
LVEQGPEHLLNQRVVGEINAVCGYCRFCRRGQPTHCENRTVLGIVNRHGAFAEYLCLPVE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHEECCC
NLHVVPENVPTDAATFTEPLAAALEIQQQIILSRDDRVLVVGDGKLGQLVAQTLALTGCE
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCE
LLVVGRHQEKLANLKVRGVQTGLVDAVQDRAFDIVVECTGNPEGFAIARRALRPRGTLVL
EEEECCCHHHHHCEEEECCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEE
KSTYAGNLSLDASSLVVDEITLIGSRCGVFPPALELLATGKVDVEPLIHSQYPLTQGLLA
EEECCCCEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHH
FEEAQRRGVLKVLLEMGN
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA