Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 17231871

GI number: 17231871

Start: 5248068

End: 5249690

Strand: Reverse

Name: prfC [H]

Synonym: all4379

Alternate gene names: 17231871

Gene position: 5249690-5248068 (Counterclockwise)

Preceding gene: 17231873

Following gene: 17231870

Centisome position: 81.85

GC content: 45.84

Gene sequence:

>1623_bases
ATGTCAACTGAAATACAGACGGAACTCCACCAAGCAGTTGAGCTTCGCCGCAATTTTGCTATTATCTCTCACCCTGACGC
TGGTAAAACTACGCTGACAGAAAAATTACTATTGTACGGGGGTGCAATTCACGAAGCTGGGGCAGTCAAAGCCCGCCGCG
CTCAACGCAAAGCTACCTCAGACTGGATGGCAATGGAACAACAACGAGGTATTTCCATTACATCTACAGTATTACAGTTT
GAGTACCAAAATTGCCAGATTAATTTACTCGACACACCCGGACACCAAGATTTTAGTGAAGACACTTATCGCACCTTGGC
GGCGGCTGATAATGCGGTGATGTTAATTGACGTGGCTAAAGGTTTGGAACCCCAAACGCGCAAGTTATTTGAGGTGTGTA
AATTACGAGGTTTACCAATCTTCACATTTATTAACAAACTTGACCGTCCAGGTAGGGAACCACTGGAGTTATTAGACGAG
ATTGAGCAAGAATTGGGTTTACAGACCTATGCTGTTAACTGGCCGATTGGCATGGGCGATCGCTTCAAGGGAGTATTTGA
TCGTCACAAGCAACAAATCCACCTGTTTGAACGTAGCGCTCACGGTAGCAAGGAAGCCCGTGATACAACAGTTGAGTTGG
GCGACCCCAGAATTGAAGAGCTATTAGAAGAAGATTTGTATTACCAACTGAAAAACGATCTAGAACTTTTAGAAGGTGTC
GGCCCGGAACTAGATTTAGATTTGGTACATCAAGGCAAGATGACACCAGTTTTCTTTGGTAGTGCCATGACAAACTTTGG
GGTTGAGTTATTCCTCAAGTACTTCCTAGACTATGCCCTAAAACCCGGTGTCCACATCAGCAGCGTTGGTGAAGTACCTC
CCACATACCCAGAGTTTTCTGGTTTTGTCTTTAAATTACAAGCCAATATGGACCCCAAGCATCGAGATCGGGTTGCTTTT
ATCCGGGTCTGCACTGGTAAGTTTGAAAAAGATATGACAGTTAATCACGCCCGGACTGGCAAAATTGTGCGCCTATCGCG
TCCACAAAAGCTCTTTGCCCAAGAACGGGAATCGATTGATGTAGCTTATCCAGGTGATGTGATCGGTTTGAATAATCCAG
GTGTTTTTGCGATCGGCGATACAATTTACACGGGGCAAAAGCTGGAATATGAAGGAATTCCGTATTTTTCACCAGAACTA
TTTGCGACTCTTCGCAACCCAAACCCCTCGAAATTTAAGCAATTTCAGAAAGGCATCTCCGAATTGCGCGAAGAGGGTGC
AGTGCAAATTATGTACTCTAACGATGAAGCCAAGCGCGACCCAATTATGGCAGCTGTGGGTCAGCTGCAATTCGAGGTGG
TGCAGTTTCGCTTACAAAACGAGTATGGTGTAGAAACCATTCTGGAACTACTACCTTACAGCGTCGCCCGTTGGGTCGAG
GGCGGTTGGGAAGCTTTGAACAAGGTGGGACGTATATTCAACACCACCACAGTCAAAGACAGCATGAACCGCCCAGTTTT
ACTGTTCCGCAACGAGTGGAACTGTCAACAGTTACAGGGAGATCATCCAGAGTTAAAACTCAGCGCGATCGCCCCAGTTT
TTTCTAGCCAAACAGTAGAATAA

Upstream 100 bases:

>100_bases
GAATAACCCTATCACGAGCATCGTTGTTTTAAGATGAAAAGACTCGCTAAAGTAGAAAAAAATTAAGTTAAGTTACGTAA
CAAGGAAGTTAATACATTTT

Downstream 100 bases:

>100_bases
TTCAAAATTCAAAATTAATAATACTAGACGTATTAATTACGAATTGCGAATCAAGAATTAGAAATTGCTAGGCTGGAATT
TACTGGCATTTAAAATCAGT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSTEIQTELHQAVELRRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRKATSDWMAMEQQRGISITSTVLQF
EYQNCQINLLDTPGHQDFSEDTYRTLAAADNAVMLIDVAKGLEPQTRKLFEVCKLRGLPIFTFINKLDRPGREPLELLDE
IEQELGLQTYAVNWPIGMGDRFKGVFDRHKQQIHLFERSAHGSKEARDTTVELGDPRIEELLEEDLYYQLKNDLELLEGV
GPELDLDLVHQGKMTPVFFGSAMTNFGVELFLKYFLDYALKPGVHISSVGEVPPTYPEFSGFVFKLQANMDPKHRDRVAF
IRVCTGKFEKDMTVNHARTGKIVRLSRPQKLFAQERESIDVAYPGDVIGLNNPGVFAIGDTIYTGQKLEYEGIPYFSPEL
FATLRNPNPSKFKQFQKGISELREEGAVQIMYSNDEAKRDPIMAAVGQLQFEVVQFRLQNEYGVETILELLPYSVARWVE
GGWEALNKVGRIFNTTTVKDSMNRPVLLFRNEWNCQQLQGDHPELKLSAIAPVFSSQTVE

Sequences:

>Translated_540_residues
MSTEIQTELHQAVELRRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRKATSDWMAMEQQRGISITSTVLQF
EYQNCQINLLDTPGHQDFSEDTYRTLAAADNAVMLIDVAKGLEPQTRKLFEVCKLRGLPIFTFINKLDRPGREPLELLDE
IEQELGLQTYAVNWPIGMGDRFKGVFDRHKQQIHLFERSAHGSKEARDTTVELGDPRIEELLEEDLYYQLKNDLELLEGV
GPELDLDLVHQGKMTPVFFGSAMTNFGVELFLKYFLDYALKPGVHISSVGEVPPTYPEFSGFVFKLQANMDPKHRDRVAF
IRVCTGKFEKDMTVNHARTGKIVRLSRPQKLFAQERESIDVAYPGDVIGLNNPGVFAIGDTIYTGQKLEYEGIPYFSPEL
FATLRNPNPSKFKQFQKGISELREEGAVQIMYSNDEAKRDPIMAAVGQLQFEVVQFRLQNEYGVETILELLPYSVARWVE
GGWEALNKVGRIFNTTTVKDSMNRPVLLFRNEWNCQQLQGDHPELKLSAIAPVFSSQTVE
>Mature_539_residues
STEIQTELHQAVELRRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRKATSDWMAMEQQRGISITSTVLQFE
YQNCQINLLDTPGHQDFSEDTYRTLAAADNAVMLIDVAKGLEPQTRKLFEVCKLRGLPIFTFINKLDRPGREPLELLDEI
EQELGLQTYAVNWPIGMGDRFKGVFDRHKQQIHLFERSAHGSKEARDTTVELGDPRIEELLEEDLYYQLKNDLELLEGVG
PELDLDLVHQGKMTPVFFGSAMTNFGVELFLKYFLDYALKPGVHISSVGEVPPTYPEFSGFVFKLQANMDPKHRDRVAFI
RVCTGKFEKDMTVNHARTGKIVRLSRPQKLFAQERESIDVAYPGDVIGLNNPGVFAIGDTIYTGQKLEYEGIPYFSPELF
ATLRNPNPSKFKQFQKGISELREEGAVQIMYSNDEAKRDPIMAAVGQLQFEVVQFRLQNEYGVETILELLPYSVARWVEG
GWEALNKVGRIFNTTTVKDSMNRPVLLFRNEWNCQQLQGDHPELKLSAIAPVFSSQTVE

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=29.253112033195, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI19923640, Length=505, Percent_Identity=26.1386138613861, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI25306283, Length=458, Percent_Identity=27.0742358078603, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=289, Percent_Identity=30.1038062283737, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157426893, Length=156, Percent_Identity=32.6923076923077, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.1167883211679, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.2013422818792, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=33.6206896551724, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=33.6206896551724, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=33.6206896551724, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790835, Length=473, Percent_Identity=50.9513742071882, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789738, Length=491, Percent_Identity=28.3095723014257, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.8993288590604, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=31.6939890710383, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=478, Percent_Identity=28.4518828451883, Blast_Score=168, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=160, Percent_Identity=36.875, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=34.0740740740741, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=150, Percent_Identity=32.6666666666667, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=150, Percent_Identity=32.6666666666667, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=29.8136645962733, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=27.7661795407098, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322359, Length=506, Percent_Identity=24.901185770751, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=154, Percent_Identity=32.4675324675325, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=171, Percent_Identity=30.9941520467836, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=171, Percent_Identity=30.9941520467836, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=490, Percent_Identity=30, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI221458488, Length=512, Percent_Identity=24.21875, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI78706572, Length=162, Percent_Identity=37.6543209876543, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=34.5070422535211, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357743, Length=151, Percent_Identity=30.4635761589404, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 61272; Mature: 61140

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIQTELHQAVELRRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRKATS
CCCCHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHH
DWMAMEQQRGISITSTVLQFEYQNCQINLLDTPGHQDFSEDTYRTLAAADNAVMLIDVAK
HHHHHHHHCCCCCEEHHHEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECC
GLEPQTRKLFEVCKLRGLPIFTFINKLDRPGREPLELLDEIEQELGLQTYAVNWPIGMGD
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCC
RFKGVFDRHKQQIHLFERSAHGSKEARDTTVELGDPRIEELLEEDLYYQLKNDLELLEGV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
GPELDLDLVHQGKMTPVFFGSAMTNFGVELFLKYFLDYALKPGVHISSVGEVPPTYPEFS
CCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCC
GFVFKLQANMDPKHRDRVAFIRVCTGKFEKDMTVNHARTGKIVRLSRPQKLFAQERESID
CEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCEE
VAYPGDVIGLNNPGVFAIGDTIYTGQKLEYEGIPYFSPELFATLRNPNPSKFKQFQKGIS
EECCCCEEECCCCCEEEECCEEECCCEEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHH
ELREEGAVQIMYSNDEAKRDPIMAAVGQLQFEVVQFRLQNEYGVETILELLPYSVARWVE
HHHHCCCEEEEECCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GGWEALNKVGRIFNTTTVKDSMNRPVLLFRNEWNCQQLQGDHPELKLSAIAPVFSSQTVE
HHHHHHHHHHHHHCCCEEHHCCCCCEEEEECCCCHHHHCCCCCCEEEEEHHHHHCCCCCC
>Mature Secondary Structure 
STEIQTELHQAVELRRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRKATS
CCCHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHH
DWMAMEQQRGISITSTVLQFEYQNCQINLLDTPGHQDFSEDTYRTLAAADNAVMLIDVAK
HHHHHHHHCCCCCEEHHHEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECC
GLEPQTRKLFEVCKLRGLPIFTFINKLDRPGREPLELLDEIEQELGLQTYAVNWPIGMGD
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCC
RFKGVFDRHKQQIHLFERSAHGSKEARDTTVELGDPRIEELLEEDLYYQLKNDLELLEGV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
GPELDLDLVHQGKMTPVFFGSAMTNFGVELFLKYFLDYALKPGVHISSVGEVPPTYPEFS
CCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCC
GFVFKLQANMDPKHRDRVAFIRVCTGKFEKDMTVNHARTGKIVRLSRPQKLFAQERESID
CEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCEE
VAYPGDVIGLNNPGVFAIGDTIYTGQKLEYEGIPYFSPELFATLRNPNPSKFKQFQKGIS
EECCCCEEECCCCCEEEECCEEECCCEEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHH
ELREEGAVQIMYSNDEAKRDPIMAAVGQLQFEVVQFRLQNEYGVETILELLPYSVARWVE
HHHHCCCEEEEECCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GGWEALNKVGRIFNTTTVKDSMNRPVLLFRNEWNCQQLQGDHPELKLSAIAPVFSSQTVE
HHHHHHHHHHHHHCCCEEHHCCCCCEEEEECCCCHHHHCCCCCCEEEEEHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA