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The map label for this gene is mdh [H]

Identifier: 17231814

GI number: 17231814

Start: 5174385

End: 5175239

Strand: Direct

Name: mdh [H]

Synonym: alr4322

Alternate gene names: 17231814

Gene position: 5174385-5175239 (Clockwise)

Preceding gene: 17231813

Following gene: 17231815

Centisome position: 80.68

GC content: 42.69

Gene sequence:

>855_bases
GTGGTGCTATTAGATATTGTTGAGGGTATACCTCAAGGATTAGCACTAGATTTATTAGAAGCTAGGGGTATTGAATTGCA
TAATCGTCAGATTATCGGCACAAATAATTATGCTGATACCTCTGGTTCGCAAATCGTTGTCATAACAGCCGGATTTCCCC
GCAAGCCAGGGATGAGTCGGGATGATTTGTTGAGAACTAACGCCAAGATTGTTATTGAGGCAGCAAAGCAAGCGATCGCC
TACTCTCCCTATGCTATTTTTATCGTAGTCACCAATCCTTTGGATGTGATGACTTATTTAGCTTGGGAAGCCACAGGTTT
ACCCCGTAACCGCATCATGGGTATGGCTGGGGTGTTAGACTCGGCAAGATTTGAAACTTTTATTGCTTTAGAATTAGGGG
TATTACCTGCGGATGTCAAAGCAATGGTATTGGGTAGCCACGGGGATTTGATGGTTCCCCTGTCTCGTTACGCCACAGTT
AATGGTATCCCCATTACACAATTGTTGGATGCAGTCACAATAGAAAGGTTAGTGGAAAGAACCCGTAATGGTGGTGCGGA
AATTGTGGAATTGATGCAGACTGGTGGCGCGTTTTTTGCACCTGCATCAGCTACAAGTTTGATGGTGGAATCAATTTTAT
TGAATCAGTCCCGGCTGTTACCTGTGTCTATATATCTGCAAGGCGAATACGATTTAAAAGATGTAGTGATTGGTGTTCCT
TGTCGGTTGGGATTAAATGGAATTGAGAGTGTAATAGAATTAAATCTCAGTGATAGTGAAAGAGAAGCTTTACATATCTC
GGCTAAATCAGTGCAGAAAAATATTGAGCGATGGCGTTCTCTCCAGAATAGCTGA

Upstream 100 bases:

>100_bases
CTCCCCAATTTTGCATTGTCTACCTCGTGTCGCCATTATTGGTGCTGGTAGGGTTGGTAGCACTTTAGCTCAACGTATTG
CTGAGAAAAATTTAGCAGAT

Downstream 100 bases:

>100_bases
TATCAATAAAATTTGAGCGTCACTTGATAAGTATACTCATTACTAATACTTCTTGATGATATAAGAATGTCTAACCTGAG
ATATATATTTTAAAAATATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MVLLDIVEGIPQGLALDLLEARGIELHNRQIIGTNNYADTSGSQIVVITAGFPRKPGMSRDDLLRTNAKIVIEAAKQAIA
YSPYAIFIVVTNPLDVMTYLAWEATGLPRNRIMGMAGVLDSARFETFIALELGVLPADVKAMVLGSHGDLMVPLSRYATV
NGIPITQLLDAVTIERLVERTRNGGAEIVELMQTGGAFFAPASATSLMVESILLNQSRLLPVSIYLQGEYDLKDVVIGVP
CRLGLNGIESVIELNLSDSEREALHISAKSVQKNIERWRSLQNS

Sequences:

>Translated_284_residues
MVLLDIVEGIPQGLALDLLEARGIELHNRQIIGTNNYADTSGSQIVVITAGFPRKPGMSRDDLLRTNAKIVIEAAKQAIA
YSPYAIFIVVTNPLDVMTYLAWEATGLPRNRIMGMAGVLDSARFETFIALELGVLPADVKAMVLGSHGDLMVPLSRYATV
NGIPITQLLDAVTIERLVERTRNGGAEIVELMQTGGAFFAPASATSLMVESILLNQSRLLPVSIYLQGEYDLKDVVIGVP
CRLGLNGIESVIELNLSDSEREALHISAKSVQKNIERWRSLQNS
>Mature_284_residues
MVLLDIVEGIPQGLALDLLEARGIELHNRQIIGTNNYADTSGSQIVVITAGFPRKPGMSRDDLLRTNAKIVIEAAKQAIA
YSPYAIFIVVTNPLDVMTYLAWEATGLPRNRIMGMAGVLDSARFETFIALELGVLPADVKAMVLGSHGDLMVPLSRYATV
NGIPITQLLDAVTIERLVERTRNGGAEIVELMQTGGAFFAPASATSLMVESILLNQSRLLPVSIYLQGEYDLKDVVIGVP
CRLGLNGIESVIELNLSDSEREALHISAKSVQKNIERWRSLQNS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=287, Percent_Identity=33.1010452961672, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI221136809, Length=287, Percent_Identity=33.1010452961672, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI260099723, Length=285, Percent_Identity=32.280701754386, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI5031857, Length=285, Percent_Identity=32.280701754386, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI291575128, Length=285, Percent_Identity=32.280701754386, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI4557032, Length=285, Percent_Identity=32.280701754386, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI15082234, Length=278, Percent_Identity=31.6546762589928, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI9257228, Length=278, Percent_Identity=32.3741007194245, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4504973, Length=278, Percent_Identity=32.3741007194245, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=32.4742268041237, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI260099725, Length=168, Percent_Identity=35.1190476190476, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI207028494, Length=197, Percent_Identity=32.994923857868, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI103472011, Length=275, Percent_Identity=26.1818181818182, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI21735621, Length=162, Percent_Identity=35.1851851851852, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789632, Length=251, Percent_Identity=29.8804780876494, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535107, Length=280, Percent_Identity=28.2142857142857, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17554310, Length=253, Percent_Identity=29.6442687747036, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=281, Percent_Identity=27.7580071174377, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322765, Length=253, Percent_Identity=28.4584980237154, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17136226, Length=276, Percent_Identity=32.2463768115942, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45550422, Length=278, Percent_Identity=28.4172661870504, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24647881, Length=296, Percent_Identity=30.4054054054054, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24663599, Length=251, Percent_Identity=31.8725099601594, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663595, Length=282, Percent_Identity=29.7872340425532, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 30869; Mature: 30869

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLDIVEGIPQGLALDLLEARGIELHNRQIIGTNNYADTSGSQIVVITAGFPRKPGMSR
CEEEEHHHCCCCCHHHHHHHHCCCEEECCEEEECCCCCCCCCCEEEEEECCCCCCCCCCH
DDLLRTNAKIVIEAAKQAIAYSPYAIFIVVTNPLDVMTYLAWEATGLPRNRIMGMAGVLD
HHHHHCCCCEEEEEHHHHHEECCEEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHC
SARFETFIALELGVLPADVKAMVLGSHGDLMVPLSRYATVNGIPITQLLDAVTIERLVER
CHHHHEEEEEEECCCCCCCEEEEEECCCCEEEEHHHHEEECCCCHHHHHHHHHHHHHHHH
TRNGGAEIVELMQTGGAFFAPASATSLMVESILLNQSRLLPVSIYLQGEYDLKDVVIGVP
HCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEEEECC
CRLGLNGIESVIELNLSDSEREALHISAKSVQKNIERWRSLQNS
CCCCCHHHHHHEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLLDIVEGIPQGLALDLLEARGIELHNRQIIGTNNYADTSGSQIVVITAGFPRKPGMSR
CEEEEHHHCCCCCHHHHHHHHCCCEEECCEEEECCCCCCCCCCEEEEEECCCCCCCCCCH
DDLLRTNAKIVIEAAKQAIAYSPYAIFIVVTNPLDVMTYLAWEATGLPRNRIMGMAGVLD
HHHHHCCCCEEEEEHHHHHEECCEEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHC
SARFETFIALELGVLPADVKAMVLGSHGDLMVPLSRYATVNGIPITQLLDAVTIERLVER
CHHHHEEEEEEECCCCCCCEEEEEECCCCEEEEHHHHEEECCCCHHHHHHHHHHHHHHHH
TRNGGAEIVELMQTGGAFFAPASATSLMVESILLNQSRLLPVSIYLQGEYDLKDVVIGVP
HCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEEEECC
CRLGLNGIESVIELNLSDSEREALHISAKSVQKNIERWRSLQNS
CCCCCHHHHHHEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA