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The map label for this gene is gdhA [H]

Identifier: 17231747

GI number: 17231747

Start: 5104415

End: 5105728

Strand: Direct

Name: gdhA [H]

Synonym: alr4255

Alternate gene names: 17231747

Gene position: 5104415-5105728 (Clockwise)

Preceding gene: 17231743

Following gene: 17231750

Centisome position: 79.59

GC content: 47.56

Gene sequence:

>1314_bases
ATGCGGCGAATAGGAGAAAAACATATGGTTTCAATGCCGACCCTACCTTTGGAAGGTGCTAGTCCAGCACATATTTGTCC
ATTTGATCAAGCTTGTAGCTACTTGGAAGCAGCCGCAAAAGAGTTGAGGCTAGATCAAGGGGCGCTGGAAATTCTCAGTC
ACCCACGTAAAGTTGTGACAGTTTCTATACCTGTGAGATTAGATAACGGTGACATCCGGGTGCTAGCAGGACATCGAGTA
CAGCATTCTGATATATTAGGCCCCTACAAAGGTGGAACGCGTTACCATCCAGCTGTGACACTGCGGGAAGTATCTGCTCT
AGCAATGCTAATGACTTGGAAATGTGCGCTATTGGGTATTCCTTATGGTGGTGCGAAGGGAGGTATTGCTATAGATCCCG
AACACTACAGTGTAGGTGAATTAGAGAGAATTACTCGTCGTTATACAAGTGAATTGATTAAAGATATCGGCCCTTCCGTA
GATATACCAGCGCCAGATGTGGGAACTTCCGCCCGTGAGATGGCTTGGATGATGGACACTTATTCTATGAATGTTGGTCA
CGCAGTCCCAGGGGTTGTCACAGGTAAGCCAATTTCTATCGGTGGTTCACGGGGACGGGAAATGGCAACTGGACGCGGTG
TAATGATTATTGTCCGTGAAGCACTGGCGGATATGGGCAGAACCTTAGAGGGAGTCAAAGTAGTTATTCAAGGTTTTGGC
AATGTAGGCGGTGCTGCGGCTGAATTACTGTACCAAGCTGGAGCGAAAATTCTTGCTGTTTCTACAGGTGCAGGAGGTTT
ATTTGCGTCTGATGGTCTGGATATTCCGGCATTAAAAGCCTACGCTGCGGAAAATCATCGGAGTATTGTGGGTTTTCCCC
AAGCAGTACCAATTAGTAATGCAGAGTTGCTAACTTTACCTTGCGATGTTTTGATACCCGCAGCTTTGGAAAACCAAATC
ACTGAGGAAAATGTAGACCAGATACAGGCGCAAATTGTGGCGGAAGCTGCTAATGGCCCGGTGACTTTGGAGGCTAACCG
AGTTCTAGAAGGGCGGGGTGTGACTGTACTACCAGACATCTTGGCGAATGCTGGGGGTGTGGTGGTGAGTTATTTAGAGT
GGGTGCAAGGTCTTTCTTACCTATTTTGGGATGAGGAGCGTGTCAATCGGGAAATGGAACACTTGATGGTGCAAGCTTAT
CATCAAGTTATTAAACAGTCACAGATGCGGCAAGTTCCTTTGCGATTAGCCGCTTATACCTTGGGTGTGGGTAGGGTTGC
TCAGGCGTTGAGCGATCGCGGTCTTTATCCTTGA

Upstream 100 bases:

>100_bases
GCTTTTTTCTATAAAACATAAAAATGTAACAAAAACTACAAAATCACCATTTTTCTGAAAAACCAAAGATAAGCTAGGCA
TATATATCTGATTCGCCAAA

Downstream 100 bases:

>100_bases
GGAAGGGACTGGGGATTGGGGATTGGGGACTGGGTATGAAATAAACTCTTTATTCTCTCTGCGCCTCTGCGCCACCCTGC
GGGTTCGCTCTTAGCGTTCT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MRRIGEKHMVSMPTLPLEGASPAHICPFDQACSYLEAAAKELRLDQGALEILSHPRKVVTVSIPVRLDNGDIRVLAGHRV
QHSDILGPYKGGTRYHPAVTLREVSALAMLMTWKCALLGIPYGGAKGGIAIDPEHYSVGELERITRRYTSELIKDIGPSV
DIPAPDVGTSAREMAWMMDTYSMNVGHAVPGVVTGKPISIGGSRGREMATGRGVMIIVREALADMGRTLEGVKVVIQGFG
NVGGAAAELLYQAGAKILAVSTGAGGLFASDGLDIPALKAYAAENHRSIVGFPQAVPISNAELLTLPCDVLIPAALENQI
TEENVDQIQAQIVAEAANGPVTLEANRVLEGRGVTVLPDILANAGGVVVSYLEWVQGLSYLFWDEERVNREMEHLMVQAY
HQVIKQSQMRQVPLRLAAYTLGVGRVAQALSDRGLYP

Sequences:

>Translated_437_residues
MRRIGEKHMVSMPTLPLEGASPAHICPFDQACSYLEAAAKELRLDQGALEILSHPRKVVTVSIPVRLDNGDIRVLAGHRV
QHSDILGPYKGGTRYHPAVTLREVSALAMLMTWKCALLGIPYGGAKGGIAIDPEHYSVGELERITRRYTSELIKDIGPSV
DIPAPDVGTSAREMAWMMDTYSMNVGHAVPGVVTGKPISIGGSRGREMATGRGVMIIVREALADMGRTLEGVKVVIQGFG
NVGGAAAELLYQAGAKILAVSTGAGGLFASDGLDIPALKAYAAENHRSIVGFPQAVPISNAELLTLPCDVLIPAALENQI
TEENVDQIQAQIVAEAANGPVTLEANRVLEGRGVTVLPDILANAGGVVVSYLEWVQGLSYLFWDEERVNREMEHLMVQAY
HQVIKQSQMRQVPLRLAAYTLGVGRVAQALSDRGLYP
>Mature_437_residues
MRRIGEKHMVSMPTLPLEGASPAHICPFDQACSYLEAAAKELRLDQGALEILSHPRKVVTVSIPVRLDNGDIRVLAGHRV
QHSDILGPYKGGTRYHPAVTLREVSALAMLMTWKCALLGIPYGGAKGGIAIDPEHYSVGELERITRRYTSELIKDIGPSV
DIPAPDVGTSAREMAWMMDTYSMNVGHAVPGVVTGKPISIGGSRGREMATGRGVMIIVREALADMGRTLEGVKVVIQGFG
NVGGAAAELLYQAGAKILAVSTGAGGLFASDGLDIPALKAYAAENHRSIVGFPQAVPISNAELLTLPCDVLIPAALENQI
TEENVDQIQAQIVAEAANGPVTLEANRVLEGRGVTVLPDILANAGGVVVSYLEWVQGLSYLFWDEERVNREMEHLMVQAY
HQVIKQSQMRQVPLRLAAYTLGVGRVAQALSDRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=380, Percent_Identity=43.421052631579, Blast_Score=304, Evalue=9e-83,
Organism=Homo sapiens, GI4885281, Length=351, Percent_Identity=44.7293447293447, Blast_Score=299, Evalue=3e-81,
Organism=Escherichia coli, GI1788059, Length=403, Percent_Identity=33.0024813895782, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17544676, Length=389, Percent_Identity=38.560411311054, Blast_Score=274, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6319256, Length=427, Percent_Identity=29.2740046838407, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=385, Percent_Identity=30.9090909090909, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45549226, Length=376, Percent_Identity=42.0212765957447, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI45553475, Length=367, Percent_Identity=43.0517711171662, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24649283, Length=348, Percent_Identity=41.3793103448276, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24649460, Length=311, Percent_Identity=43.7299035369775, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46863; Mature: 46863

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIGEKHMVSMPTLPLEGASPAHICPFDQACSYLEAAAKELRLDQGALEILSHPRKVVT
CCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEE
VSIPVRLDNGDIRVLAGHRVQHSDILGPYKGGTRYHPAVTLREVSALAMLMTWKCALLGI
EEEEEEECCCCEEEEECCCEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHEEC
PYGGAKGGIAIDPEHYSVGELERITRRYTSELIKDIGPSVDIPAPDVGTSAREMAWMMDT
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
YSMNVGHAVPGVVTGKPISIGGSRGREMATGRGVMIIVREALADMGRTLEGVKVVIQGFG
HCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCC
NVGGAAAELLYQAGAKILAVSTGAGGLFASDGLDIPALKAYAAENHRSIVGFPQAVPISN
CCCHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCHHHHHHHCCCCEEECCCCCCCCCC
AELLTLPCDVLIPAALENQITEENVDQIQAQIVAEAANGPVTLEANRVLEGRGVTVLPDI
CEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCEEEHHH
LANAGGVVVSYLEWVQGLSYLFWDEERVNREMEHLMVQAYHQVIKQSQMRQVPLRLAAYT
HCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVGRVAQALSDRGLYP
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRIGEKHMVSMPTLPLEGASPAHICPFDQACSYLEAAAKELRLDQGALEILSHPRKVVT
CCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEE
VSIPVRLDNGDIRVLAGHRVQHSDILGPYKGGTRYHPAVTLREVSALAMLMTWKCALLGI
EEEEEEECCCCEEEEECCCEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHEEC
PYGGAKGGIAIDPEHYSVGELERITRRYTSELIKDIGPSVDIPAPDVGTSAREMAWMMDT
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
YSMNVGHAVPGVVTGKPISIGGSRGREMATGRGVMIIVREALADMGRTLEGVKVVIQGFG
HCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCC
NVGGAAAELLYQAGAKILAVSTGAGGLFASDGLDIPALKAYAAENHRSIVGFPQAVPISN
CCCHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCHHHHHHHCCCCEEECCCCCCCCCC
AELLTLPCDVLIPAALENQITEENVDQIQAQIVAEAANGPVTLEANRVLEGRGVTVLPDI
CEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCEEEHHH
LANAGGVVVSYLEWVQGLSYLFWDEERVNREMEHLMVQAYHQVIKQSQMRQVPLRLAAYT
HCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVGRVAQALSDRGLYP
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]