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The map label for this gene is murD

Identifier: 17231604

GI number: 17231604

Start: 4955215

End: 4956603

Strand: Direct

Name: murD

Synonym: alr4112

Alternate gene names: 17231604

Gene position: 4955215-4956603 (Clockwise)

Preceding gene: 17231603

Following gene: 17231606

Centisome position: 77.26

GC content: 47.59

Gene sequence:

>1389_bases
ATGTCCAAAGCCCATGTAGTTGGATTAGGAAAGTCCGGTGTTGCTGCGGCGAGATTGTTGAAACGGGAAGGTTGGGAGGT
AGTTCTGAGCGACAGTAACACCTCCGATACCCTCCTCAAACAGCAACAAGAACTCGCTAAAGAACAAATCACAGTTGAAT
TAGGGTATTCATTAGATTTTGCTGGTGCTTTACCTGATTTGATTATTGTGAGTCCCGGCGTTCCTTGGGACATTCCCGAC
TTAGTCAAAGCGCGAGATTTGGGCATAGAAACTATTGGCGAAATGGAACTCGCTTGGCGACATTTAAAGTCTTTGCCTTG
GGTAGGAATTACCGGCACTAACGGCAAAACCACCACCACAGCCTTAATTGCCGCTATCTTTCAAGCAGCCGGATTCGATG
CCCCCGCTTGCGGTAACATTGGCTATGCGGCTTGTGAAGTGGCTCTAGCAGAAATACCCCCCGATTGGATAATTGGGGAA
ATGAGCAGCTATCAAATAGAATCGTCCGTCACCCTAGCCCCTCATATTAGTATCTGGACAACCTTCACCCCAGATCACCT
AGCCCGTCACAAGACTTTAGAAAACTATTACGACATTAAAGCCAAGTTATTACGTCAATCCCATTTACAAGTGTTCAATG
GTGATGATGCCTACTTATCTAAGATAGGTGCAAGTCATTGGCCGGATGCCTATTGGACAAGTGTGCAAGGGAAAGAATCC
CTACTTGGTGAAAAAGGCTTTTATATTGAAGATGGCTGGGTGGTAGAACAATTGCCAAACTCCCCACCCCAAAGAATTGT
CGAAGCCTCAGCTCTACGCATGGTAGGGGCGCACAACCTGCAAAATCTCCTCATGGCAGTAGCCGCAGCCCGTTTAGCAG
ATATTTCCCCCAATGCCATTGACAAGGCGGTGCGAGAATTTCCAGGGGTTGCTCATCGCTTAGAACACATCTGCACTTGG
GAAGGCATCGACTTTATCAACGACAGCAAAGCCACAAACTACGATGCGGCGGAAGTAGGACTAGCATCGGTGAAAAGCCC
AGTGGTTTTAATAGCTGGCGGTGAAGCCAAACCAGGGGATGATACAGCTTGGTTGGCGAAAATCCAAGCGCAAACATCAG
CCGTGTTACTTATTGGTAGTGCTGCACCAGCATTCGCCCAACGCCTAAAAGAAGTTGGGTATACCCATTATGAAATCGTG
GAGACGATGGAAAAAGCGGTAAGGCGATCGCTAGAATTAGCTAAACACCATCAAGCCCCCGTAGTCTTGTTATCCCCAGC
CTGCGCCAGCTTCGACCAATACCCCAATTTTGAAGCACGGGGCGATCATTTTCGGGAGTTGTGTCTGGAATTGGTAGGGG
AAAAAGAAACAAATTCAAATCATCATTAA

Upstream 100 bases:

>100_bases
CGTGAAAAATCTGTAGTATAAAGCAATAAAAAGTGGTGTAATTTTTGCCCATTAAGCGCTACCATTATGAGTGCTTAACC
AGGGAACTCTGCCACAGTCT

Downstream 100 bases:

>100_bases
TGCTGATGTACTCTCTCCAGTAGCCAAATTGAAGCGATCGCCCCCACAAAATGGGCCGCAATACCATTGATATTCCCTAC
CATCACAAACACATCCAGCG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSKAHVVGLGKSGVAAARLLKREGWEVVLSDSNTSDTLLKQQQELAKEQITVELGYSLDFAGALPDLIIVSPGVPWDIPD
LVKARDLGIETIGEMELAWRHLKSLPWVGITGTNGKTTTTALIAAIFQAAGFDAPACGNIGYAACEVALAEIPPDWIIGE
MSSYQIESSVTLAPHISIWTTFTPDHLARHKTLENYYDIKAKLLRQSHLQVFNGDDAYLSKIGASHWPDAYWTSVQGKES
LLGEKGFYIEDGWVVEQLPNSPPQRIVEASALRMVGAHNLQNLLMAVAAARLADISPNAIDKAVREFPGVAHRLEHICTW
EGIDFINDSKATNYDAAEVGLASVKSPVVLIAGGEAKPGDDTAWLAKIQAQTSAVLLIGSAAPAFAQRLKEVGYTHYEIV
ETMEKAVRRSLELAKHHQAPVVLLSPACASFDQYPNFEARGDHFRELCLELVGEKETNSNHH

Sequences:

>Translated_462_residues
MSKAHVVGLGKSGVAAARLLKREGWEVVLSDSNTSDTLLKQQQELAKEQITVELGYSLDFAGALPDLIIVSPGVPWDIPD
LVKARDLGIETIGEMELAWRHLKSLPWVGITGTNGKTTTTALIAAIFQAAGFDAPACGNIGYAACEVALAEIPPDWIIGE
MSSYQIESSVTLAPHISIWTTFTPDHLARHKTLENYYDIKAKLLRQSHLQVFNGDDAYLSKIGASHWPDAYWTSVQGKES
LLGEKGFYIEDGWVVEQLPNSPPQRIVEASALRMVGAHNLQNLLMAVAAARLADISPNAIDKAVREFPGVAHRLEHICTW
EGIDFINDSKATNYDAAEVGLASVKSPVVLIAGGEAKPGDDTAWLAKIQAQTSAVLLIGSAAPAFAQRLKEVGYTHYEIV
ETMEKAVRRSLELAKHHQAPVVLLSPACASFDQYPNFEARGDHFRELCLELVGEKETNSNHH
>Mature_461_residues
SKAHVVGLGKSGVAAARLLKREGWEVVLSDSNTSDTLLKQQQELAKEQITVELGYSLDFAGALPDLIIVSPGVPWDIPDL
VKARDLGIETIGEMELAWRHLKSLPWVGITGTNGKTTTTALIAAIFQAAGFDAPACGNIGYAACEVALAEIPPDWIIGEM
SSYQIESSVTLAPHISIWTTFTPDHLARHKTLENYYDIKAKLLRQSHLQVFNGDDAYLSKIGASHWPDAYWTSVQGKESL
LGEKGFYIEDGWVVEQLPNSPPQRIVEASALRMVGAHNLQNLLMAVAAARLADISPNAIDKAVREFPGVAHRLEHICTWE
GIDFINDSKATNYDAAEVGLASVKSPVVLIAGGEAKPGDDTAWLAKIQAQTSAVLLIGSAAPAFAQRLKEVGYTHYEIVE
TMEKAVRRSLELAKHHQAPVVLLSPACASFDQYPNFEARGDHFRELCLELVGEKETNSNHH

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=449, Percent_Identity=30.7349665924276, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_NOSS1 (Q8YPS9)

Other databases:

- EMBL:   BA000019
- PIR:   AI2319
- RefSeq:   NP_488152.1
- ProteinModelPortal:   Q8YPS9
- STRING:   Q8YPS9
- GeneID:   1107714
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr4112
- NMPDR:   fig|103690.1.peg.4419
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   LPWVGIT
- ProtClustDB:   PRK02705
- BioCyc:   NSP103690:ALR4112-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50315; Mature: 50183

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAHVVGLGKSGVAAARLLKREGWEVVLSDSNTSDTLLKQQQELAKEQITVELGYSLDF
CCCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCEEC
AGALPDLIIVSPGVPWDIPDLVKARDLGIETIGEMELAWRHLKSLPWVGITGTNGKTTTT
CCCCCCEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
ALIAAIFQAAGFDAPACGNIGYAACEVALAEIPPDWIIGEMSSYQIESSVTLAPHISIWT
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCEEECCCEEECCCEEEEE
TFTPDHLARHKTLENYYDIKAKLLRQSHLQVFNGDDAYLSKIGASHWPDAYWTSVQGKES
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHCCCCCCCHHEECCCCHHH
LLGEKGFYIEDGWVVEQLPNSPPQRIVEASALRMVGAHNLQNLLMAVAAARLADISPNAI
HHCCCCCEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DKAVREFPGVAHRLEHICTWEGIDFINDSKATNYDAAEVGLASVKSPVVLIAGGEAKPGD
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCC
DTAWLAKIQAQTSAVLLIGSAAPAFAQRLKEVGYTHYEIVETMEKAVRRSLELAKHHQAP
CHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VVLLSPACASFDQYPNFEARGDHFRELCLELVGEKETNSNHH
EEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SKAHVVGLGKSGVAAARLLKREGWEVVLSDSNTSDTLLKQQQELAKEQITVELGYSLDF
CCCEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCEEC
AGALPDLIIVSPGVPWDIPDLVKARDLGIETIGEMELAWRHLKSLPWVGITGTNGKTTTT
CCCCCCEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
ALIAAIFQAAGFDAPACGNIGYAACEVALAEIPPDWIIGEMSSYQIESSVTLAPHISIWT
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCEEECCCEEECCCEEEEE
TFTPDHLARHKTLENYYDIKAKLLRQSHLQVFNGDDAYLSKIGASHWPDAYWTSVQGKES
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHCCCCCCCHHEECCCCHHH
LLGEKGFYIEDGWVVEQLPNSPPQRIVEASALRMVGAHNLQNLLMAVAAARLADISPNAI
HHCCCCCEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DKAVREFPGVAHRLEHICTWEGIDFINDSKATNYDAAEVGLASVKSPVVLIAGGEAKPGD
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCC
DTAWLAKIQAQTSAVLLIGSAAPAFAQRLKEVGYTHYEIVETMEKAVRRSLELAKHHQAP
CHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VVLLSPACASFDQYPNFEARGDHFRELCLELVGEKETNSNHH
EEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840