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The map label for this gene is nfi [H]

Identifier: 17231577

GI number: 17231577

Start: 4924034

End: 4924699

Strand: Reverse

Name: nfi [H]

Synonym: all4085

Alternate gene names: 17231577

Gene position: 4924699-4924034 (Counterclockwise)

Preceding gene: 17231579

Following gene: 17231576

Centisome position: 76.78

GC content: 41.29

Gene sequence:

>666_bases
ATGCAGATTTACCAACCTCATCCTTGGCCATTAACGGTGGAAGAAGCCATAACTATCCAAGAAGAATTAAGACATCAAGT
AATTACAGAAGACCAATTTACACAACCTGTGCAGTACGTCGCTGGTGTTGATATGGGTTTTGAAGCCGATGGCACAATTA
GCCGTGCAGCAGTAGCAGTACTGAGTTTTCCTGATTTACAAGTCATCGAAACGAACCTAGCATATCGTCCTACGTCATTT
CCCTACATTCCTGGTTTTCTTTCTTTCCGAGAAATACCAGCAGTACTTGATGCCCTAGCAAAAGTTCAAACAAAACCAGA
TATTATTTTGTGTGATGGTCAAGGAATCGCCCATCCTCGAAGATTAGGTATAGCTAGTCACTTAGGAGTACTGCTCAATA
TTCCCACAATTGGTGTAGCTAAATCTCTATTAATTGGTAAACATGAGGAATTAGCAGACACCAAAGGCAGTTGGCAACCA
CTTATACACAGAGGTGAAATTATTGGTGCAGTTCTACGAACTCGCGTAGGAGTAAAGCCTGTATATGTTTCTAGTGGACA
TAAAATCAGTTTACCAACCGCTATTGACTACGTATTGCGATGTACACCAAAATATCGCCTACCAGAAACTACTCGCGTAG
CCGATAAACTAGCATCCAATCGATAA

Upstream 100 bases:

>100_bases
TAACGCAACCAACAATAGTTAAAAATTTCACAAATGTCTTAAATCTCTACGTACATTTATTGAAATTGCTAAAATTATTA
TTTATAACCAATTTTTAATT

Downstream 100 bases:

>100_bases
AGTCATCAAGCCACAATATAAATTTGTTGAAGTTTGTTCTCAAAACTTGCTTGCAACTGCTCACTCATGTTAGGAATCGC
ACAGAAGGAAGTTGAAGTAG

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MQIYQPHPWPLTVEEAITIQEELRHQVITEDQFTQPVQYVAGVDMGFEADGTISRAAVAVLSFPDLQVIETNLAYRPTSF
PYIPGFLSFREIPAVLDALAKVQTKPDIILCDGQGIAHPRRLGIASHLGVLLNIPTIGVAKSLLIGKHEELADTKGSWQP
LIHRGEIIGAVLRTRVGVKPVYVSSGHKISLPTAIDYVLRCTPKYRLPETTRVADKLASNR

Sequences:

>Translated_221_residues
MQIYQPHPWPLTVEEAITIQEELRHQVITEDQFTQPVQYVAGVDMGFEADGTISRAAVAVLSFPDLQVIETNLAYRPTSF
PYIPGFLSFREIPAVLDALAKVQTKPDIILCDGQGIAHPRRLGIASHLGVLLNIPTIGVAKSLLIGKHEELADTKGSWQP
LIHRGEIIGAVLRTRVGVKPVYVSSGHKISLPTAIDYVLRCTPKYRLPETTRVADKLASNR
>Mature_221_residues
MQIYQPHPWPLTVEEAITIQEELRHQVITEDQFTQPVQYVAGVDMGFEADGTISRAAVAVLSFPDLQVIETNLAYRPTSF
PYIPGFLSFREIPAVLDALAKVQTKPDIILCDGQGIAHPRRLGIASHLGVLLNIPTIGVAKSLLIGKHEELADTKGSWQP
LIHRGEIIGAVLRTRVGVKPVYVSSGHKISLPTAIDYVLRCTPKYRLPETTRVADKLASNR

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=197, Percent_Identity=37.0558375634518, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI257467548, Length=192, Percent_Identity=31.7708333333333, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI257467550, Length=192, Percent_Identity=31.7708333333333, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI87082357, Length=203, Percent_Identity=47.2906403940887, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI71981509, Length=113, Percent_Identity=38.9380530973451, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24378; Mature: 24378

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYQPHPWPLTVEEAITIQEELRHQVITEDQFTQPVQYVAGVDMGFEADGTISRAAVAV
CCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LSFPDLQVIETNLAYRPTSFPYIPGFLSFREIPAVLDALAKVQTKPDIILCDGQGIAHPR
HCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
RLGIASHLGVLLNIPTIGVAKSLLIGKHEELADTKGSWQPLIHRGEIIGAVLRTRVGVKP
HHHHHHHHHHEEECCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCE
VYVSSGHKISLPTAIDYVLRCTPKYRLPETTRVADKLASNR
EEECCCCEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQIYQPHPWPLTVEEAITIQEELRHQVITEDQFTQPVQYVAGVDMGFEADGTISRAAVAV
CCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LSFPDLQVIETNLAYRPTSFPYIPGFLSFREIPAVLDALAKVQTKPDIILCDGQGIAHPR
HCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
RLGIASHLGVLLNIPTIGVAKSLLIGKHEELADTKGSWQPLIHRGEIIGAVLRTRVGVKP
HHHHHHHHHHEEECCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCE
VYVSSGHKISLPTAIDYVLRCTPKYRLPETTRVADKLASNR
EEECCCCEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA