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The map label for this gene is pyk2 [H]

Identifier: 17231500

GI number: 17231500

Start: 4827144

End: 4828913

Strand: Reverse

Name: pyk2 [H]

Synonym: all4008

Alternate gene names: 17231500

Gene position: 4828913-4827144 (Counterclockwise)

Preceding gene: 17231504

Following gene: 17231498

Centisome position: 75.29

GC content: 47.97

Gene sequence:

>1770_bases
ATGCAATTAAGAGATTCTGTACGCCGGACAAAAATCGTCGCTACAATTGGCCCTGCCACTAGTAGCCCCGAAATGCTGAA
AGCCATCATTGAAGCGGGTGCAACAACGCTGCGACTCAACTTTTCCCACGGTTCCCACGCCGACCATCAGCGTAGTATTC
GCCTAATTCGGCAAACCGCTTTTGAACTAAATCAGCCAGTGGCAATTCTCCAAGATTTGCAAGGGCCAAAAATTCGCTTG
GGAAAGTTTGAAAACGGGTCTATAGTTTTAGCTAAAGGCGATCGCTTCACCTTAACAAATCGCCCAGTTGTTGGTAGTCA
AGAAATTAGCTGTGTCACCTACGATTATTTGGCAGATGAGGTCCCTGTAGGTGCAAAAATCCTCCTGGATGATGGCCGTG
TAGAAATGGTGGTAGAGGATATCAACCGCGACAAAGGGGATTTGAACTGTCGGGTGACTGTAGCAGGTAAGTTATCTAAT
AACAAAGGTGTTAACTTTCCTGGCGTTTATCTCTCAATTAAAGCCATGACCGACAAAGATCGTGAGGATCTGATGTTTGG
TCTAGATCAGGGTGTTGATTGGGTAGCCCTTTCCTTTGTCCGCAATCCGCAAGACATCATTGAAATTAAAGAGCTAATTT
CCAGCACTGGTAAACAAGTGCCGGTAGTTGCCAAAATAGAAAAGCATGAAGCGATCGAACAAATGGAAGCAGTTCTCGCT
TTATGTGATGGCGTAATGGTGGCTAGAGGCGATTTAGGTGTAGAACTACCAGCAGAAGATGTCCCCGTACTACAAAAGCG
CTTAATTGCCACAGCAAACCGCTTGGGGATTCCCATCATCACTGCTACTCAAATGTTAGATAGCATGGTGAGCAATCCCC
GTCCCACCCGCGCGGAAGTGTCCGATGTAGCCAATGCCATTCTTGATGGTACAGATGCGGTCATGCTCTCCAATGAAACG
GCTGTGGGTAGCTACCCAGTGGAAGCAGTGGCGACAATGGCAAGAATTGCCGAACGCATCGAGCAGGAAGAAGCTATGGC
TAGTAAATTACGCCAAATGCGAGATAACCGCCGTTCTATTCCCAACGCCATCAGCCAAGCTGTAGGTCAAATTGCTGAAC
AGTTGGGAGCAGCAGCAATTATGACTCTCACCCAAACAGGGGCGACTGCACGCAACGTTTCCAAATTCCGCCCCCAAACA
CCAATCTTGGCAGTAACACCCCACGTTAACGTAGCTCGTCAACTACAAATGGTGTGGGGCGTAAAACCTTTGTTGGTCTT
AGAATTACCTTCCACTGGTCAAACTTTCCAAGCCGCCATTAACGTCGCCCAAGAGCATAGTTTACTGTTTGAAGGTGATT
TAGTCGTGATGACAGCCGGGACGTTACAAGGGGTATCTGGCTCAACGGACTTGATTAAAGTTGAAGTGGTAACGGCAGTT
CTAGGACAGGGAATTGGTCTAGGACAGGGTTTGGTAAGCGGTTGTGCTAGGGTTGCTCACACCGGGATGGATGTGGGGAA
CTTTAACGCTGGTGATATTTTGGTTGCACCCCGCACAGGTGCAGATTTTGTCGAGGCGATTCGCAAAGCCGGCGGGATTA
TTACAGAGGATGAAAGTTTAACTAGTCATGCGGCGGTGATTGGTTTACGCCTGGGTGTGCCGGTGATTGTAGGTGTGAAG
AAAGCCACGCAAGTTATCCGTGATGGAGCAATTTTGACTCTGGATTTGCAACGAGGTTTAGTTTATTCCGGAGCAGTAGG
AACTCCTTAA

Upstream 100 bases:

>100_bases
ACATCTCCCATAGTTTGAGTCACGGATATAACAGTGACTGAAAACTAAATGCCCCTTAATTTACTATGACTCACTAGATA
GCTCCCTAGAATCAGCCCCT

Downstream 100 bases:

>100_bases
TTAACTTAAACAAGGGTGTAGGGGTAGAGGGGTGTAGGGGTGTAAATGTAAATGTTTAAAACCCTTACACCCATTCTCAA
CGCAACTCTGTGAAAATCGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MQLRDSVRRTKIVATIGPATSSPEMLKAIIEAGATTLRLNFSHGSHADHQRSIRLIRQTAFELNQPVAILQDLQGPKIRL
GKFENGSIVLAKGDRFTLTNRPVVGSQEISCVTYDYLADEVPVGAKILLDDGRVEMVVEDINRDKGDLNCRVTVAGKLSN
NKGVNFPGVYLSIKAMTDKDREDLMFGLDQGVDWVALSFVRNPQDIIEIKELISSTGKQVPVVAKIEKHEAIEQMEAVLA
LCDGVMVARGDLGVELPAEDVPVLQKRLIATANRLGIPIITATQMLDSMVSNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGSYPVEAVATMARIAERIEQEEAMASKLRQMRDNRRSIPNAISQAVGQIAEQLGAAAIMTLTQTGATARNVSKFRPQT
PILAVTPHVNVARQLQMVWGVKPLLVLELPSTGQTFQAAINVAQEHSLLFEGDLVVMTAGTLQGVSGSTDLIKVEVVTAV
LGQGIGLGQGLVSGCARVAHTGMDVGNFNAGDILVAPRTGADFVEAIRKAGGIITEDESLTSHAAVIGLRLGVPVIVGVK
KATQVIRDGAILTLDLQRGLVYSGAVGTP

Sequences:

>Translated_589_residues
MQLRDSVRRTKIVATIGPATSSPEMLKAIIEAGATTLRLNFSHGSHADHQRSIRLIRQTAFELNQPVAILQDLQGPKIRL
GKFENGSIVLAKGDRFTLTNRPVVGSQEISCVTYDYLADEVPVGAKILLDDGRVEMVVEDINRDKGDLNCRVTVAGKLSN
NKGVNFPGVYLSIKAMTDKDREDLMFGLDQGVDWVALSFVRNPQDIIEIKELISSTGKQVPVVAKIEKHEAIEQMEAVLA
LCDGVMVARGDLGVELPAEDVPVLQKRLIATANRLGIPIITATQMLDSMVSNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGSYPVEAVATMARIAERIEQEEAMASKLRQMRDNRRSIPNAISQAVGQIAEQLGAAAIMTLTQTGATARNVSKFRPQT
PILAVTPHVNVARQLQMVWGVKPLLVLELPSTGQTFQAAINVAQEHSLLFEGDLVVMTAGTLQGVSGSTDLIKVEVVTAV
LGQGIGLGQGLVSGCARVAHTGMDVGNFNAGDILVAPRTGADFVEAIRKAGGIITEDESLTSHAAVIGLRLGVPVIVGVK
KATQVIRDGAILTLDLQRGLVYSGAVGTP
>Mature_589_residues
MQLRDSVRRTKIVATIGPATSSPEMLKAIIEAGATTLRLNFSHGSHADHQRSIRLIRQTAFELNQPVAILQDLQGPKIRL
GKFENGSIVLAKGDRFTLTNRPVVGSQEISCVTYDYLADEVPVGAKILLDDGRVEMVVEDINRDKGDLNCRVTVAGKLSN
NKGVNFPGVYLSIKAMTDKDREDLMFGLDQGVDWVALSFVRNPQDIIEIKELISSTGKQVPVVAKIEKHEAIEQMEAVLA
LCDGVMVARGDLGVELPAEDVPVLQKRLIATANRLGIPIITATQMLDSMVSNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGSYPVEAVATMARIAERIEQEEAMASKLRQMRDNRRSIPNAISQAVGQIAEQLGAAAIMTLTQTGATARNVSKFRPQT
PILAVTPHVNVARQLQMVWGVKPLLVLELPSTGQTFQAAINVAQEHSLLFEGDLVVMTAGTLQGVSGSTDLIKVEVVTAV
LGQGIGLGQGLVSGCARVAHTGMDVGNFNAGDILVAPRTGADFVEAIRKAGGIITEDESLTSHAAVIGLRLGVPVIVGVK
KATQVIRDGAILTLDLQRGLVYSGAVGTP

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=39.3877551020408, Blast_Score=326, Evalue=4e-89,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=39.8373983739837, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=38.4928716904277, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=38.4928716904277, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=39.0688259109312, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=40.0684931506849, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=40.0684931506849, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI310128736, Length=242, Percent_Identity=37.1900826446281, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128734, Length=242, Percent_Identity=37.1900826446281, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=35.9447004608295, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=45.0526315789474, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=41.7879417879418, Blast_Score=318, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=38.241308793456, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=39.1752577319588, Blast_Score=305, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=39.1752577319588, Blast_Score=304, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=39.1752577319588, Blast_Score=304, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=39.1752577319588, Blast_Score=303, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6319279, Length=492, Percent_Identity=39.6341463414634, Blast_Score=314, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=37.6278118609407, Blast_Score=287, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24648964, Length=503, Percent_Identity=39.5626242544732, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI28571814, Length=500, Percent_Identity=39.6, Blast_Score=339, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=39.4160583941606, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=32.1649484536082, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=39.3382352941176, Blast_Score=196, Evalue=5e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62849; Mature: 62849

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRDSVRRTKIVATIGPATSSPEMLKAIIEAGATTLRLNFSHGSHADHQRSIRLIRQTA
CCCHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHH
FELNQPVAILQDLQGPKIRLGKFENGSIVLAKGDRFTLTNRPVVGSQEISCVTYDYLADE
HHCCCCHHHHHCCCCCCEEEEEECCCCEEEECCCEEEECCCCCCCCCCCEEEEHHHHHCC
VPVGAKILLDDGRVEMVVEDINRDKGDLNCRVTVAGKLSNNKGVNFPGVYLSIKAMTDKD
CCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEECCCCC
REDLMFGLDQGVDWVALSFVRNPQDIIEIKELISSTGKQVPVVAKIEKHEAIEQMEAVLA
HHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHH
LCDGVMVARGDLGVELPAEDVPVLQKRLIATANRLGIPIITATQMLDSMVSNPRPTRAEV
HHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGSYPVEAVATMARIAERIEQEEAMASKLRQMRDNRRSI
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNAISQAVGQIAEQLGAAAIMTLTQTGATARNVSKFRPQTPILAVTPHVNVARQLQMVWG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHC
VKPLLVLELPSTGQTFQAAINVAQEHSLLFEGDLVVMTAGTLQGVSGSTDLIKVEVVTAV
CCCEEEEECCCCCHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCCCEEEHHHHHHH
LGQGIGLGQGLVSGCARVAHTGMDVGNFNAGDILVAPRTGADFVEAIRKAGGIITEDESL
HHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEEECCCCH
TSHAAVIGLRLGVPVIVGVKKATQVIRDGAILTLDLQRGLVYSGAVGTP
HHHHHHHHEECCCCEEEEHHHHHHHHHCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure
MQLRDSVRRTKIVATIGPATSSPEMLKAIIEAGATTLRLNFSHGSHADHQRSIRLIRQTA
CCCHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHH
FELNQPVAILQDLQGPKIRLGKFENGSIVLAKGDRFTLTNRPVVGSQEISCVTYDYLADE
HHCCCCHHHHHCCCCCCEEEEEECCCCEEEECCCEEEECCCCCCCCCCCEEEEHHHHHCC
VPVGAKILLDDGRVEMVVEDINRDKGDLNCRVTVAGKLSNNKGVNFPGVYLSIKAMTDKD
CCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEECCCCC
REDLMFGLDQGVDWVALSFVRNPQDIIEIKELISSTGKQVPVVAKIEKHEAIEQMEAVLA
HHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHH
LCDGVMVARGDLGVELPAEDVPVLQKRLIATANRLGIPIITATQMLDSMVSNPRPTRAEV
HHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGSYPVEAVATMARIAERIEQEEAMASKLRQMRDNRRSI
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNAISQAVGQIAEQLGAAAIMTLTQTGATARNVSKFRPQTPILAVTPHVNVARQLQMVWG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHC
VKPLLVLELPSTGQTFQAAINVAQEHSLLFEGDLVVMTAGTLQGVSGSTDLIKVEVVTAV
CCCEEEEECCCCCHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCCCEEEHHHHHHH
LGQGIGLGQGLVSGCARVAHTGMDVGNFNAGDILVAPRTGADFVEAIRKAGGIITEDESL
HHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEEECCCCH
TSHAAVIGLRLGVPVIVGVKKATQVIRDGAILTLDLQRGLVYSGAVGTP
HHHHHHHHEECCCCEEEEHHHHHHHHHCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]