Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 17231448

GI number: 17231448

Start: 4774335

End: 4776425

Strand: Direct

Name: ndhF [H]

Synonym: alr3956

Alternate gene names: 17231448

Gene position: 4774335-4776425 (Clockwise)

Preceding gene: 17231447

Following gene: 17231449

Centisome position: 74.44

GC content: 47.11

Gene sequence:

>2091_bases
ATGGAAGTAATCTATCAATATGCCTGGCTAATCCCAGTATTGCCTCTGTTGGGAGCAATGCTGGTCGGTCTTGGCTTAAT
TTCGTTCAATCAAACGACAAACCGCCTGCGGCAGCTAAACGCTGTGTTGATCATTTCCCTAATGGGCGCAGCCTTGGGTT
TGTCGTCAGCTTTGCTGTGGAGTCAACTCCAAGGACACCCAACTTATCTCCGCACCCTAGAATGGGCGGCAGCAGGCAAT
TTTCACCTGACTATGGGTTACACTATCGACAATCTCACATCCCTGATGCTGGTGATTGCAACATCGGTAGCTGTTCTAGT
CATGGTTTACACGGATGGCTACATGGCTCATGACCCAGGCTACGTTAGGTTTTATGCCTATCTCAGCCTATTTGGCTCCT
CAATGTTGGGTCTAGTAGTCAGCCCTAATCTAGTCCAGATTTATATTTTCTGGGAACTGGTGGGGATGTGTTCTTACTTG
CTGGTTGGTTTCTGGTACGATCGCAAATCAGCCGCAGATGCAGCCCAAAAAGCATTTGTCACCAACCGTGTGGGTGACTT
CGGTCTACTGCTGGGCATTCTGGGGCTATTTTGGGCAACAGGCAGCTTTGATTTTCAGATTATGGGCGATCGCCTAGCTG
AACTCGTGCAAACAGGTTCTATCAGCAATTTCCTAGCTGTACTGTTTGCAATTTTAGTCTTCCTCGGCCCAGTTGCTAAA
TCTGCCCAATTCCCCCTCCATGTCTGGTTACCAGACGCGATGGAAGTCCCCACCCCCATTTCTGCCTTAATCCACGCGGC
AACGATGGTGGCGGCTGGTGTATTCCTCGTGGCGCGGATGTACCCCGTTTTTGAACACGTTCCAGCTGCAATGAACGTGA
TTGCCTTTACTGGGGCATTTACAGCATTTTTGGGTGCGACCATTGCCATTACCCAAAACGACATCAAAAAGGGCTTGGCT
TACTCCACCATCTCCCAGTTGGGTTACATGGTCATGGCGATGGGCGTAGGGGCTTACAGTGCGGGATTATTCCACCTGAT
GACCCATGCTTATTTCAAAGCGATGTTGTTCTTGGGTTCTGGTTCCGTCATTCATGGCATGGAAGCCGTTGTTGGTCATG
ACCCAGCTTTAGCGCAAGATATGCGCTTAATGGGTGGACTGCGGAAGTATATGCCTGCCACTGGATTAACATTTTTAATT
GGTTGCTTGGCGATTTCGGGGATTCCTCCCTTTGCTGGTTTCTGGTCAAAAGATGAAATTCTCGGTGCTGCTTATGCCTC
TAACCCCTTACTGTGGTTTATTGGCTGGATGACTGCTGGGATTACCGCTTTTTATATGTTTAGAATGTATTTCTCGACAT
TTGAAGGCAAATTCCGGGGGAATGACGAGAAAATCAAAGACAAGCTTCTCAAAGCTAAAACAATCCTTCTGGAACTAGAG
TCAGCAGAACCCACACCTGTTTTTGGCCCTGGGGCAATGAAAAAAGGAGAATTGGCTGCTACTGGTGGTCATCATGATGG
TCACGGTCATCACAGTAGTTCTCCCCATGAGTCGCCTTGGACAATGACTCTGCCATTGCTGATTTTGGCTATACCTTCAA
TGTTGATTGGTTTGGTAGGTACACCCTACAACAATTACTTTGAAAGGTTTATTTTTTCGCCCACAGAAAGCTTGGCTGAA
GTACTGGAAAAAGCGGCTGAATTTGATCCTAATGAGTTTTACATCATGGCTGGTGGTTCAGTCGGTGTTTCCTTGATTGG
GATTACCTTGGCTTCGCTGATGTATTTGCAGCGTAAAATCGACCCGGCGGCGATCGCTGCTAAAATTAAGCCACTCTACG
AACTATCACTTAATAAATGGTATTTCGATGACATTTACCATCGTGTCTTTGTGCTTGGTTTACGTCGCCTTGCTAGACAA
GTCATGGAAGTAGACTTCCGAGTTGTTGATGGTGCTGTGAACTTGACAGGATTTTTCACCTTAGTTAGTGGTGAAGGTTT
GAAATACCTAGAAAACGGTCGGGTGCAGTTCTATGCTCTCATCGTATTCGGGGCTGTTTTGGGCTTAGTGATTGTCTTCG
GTGTTACCTAA

Upstream 100 bases:

>100_bases
CAATTGTTATTGGGTGGGGTATTTTATCCTCAAGGGTGTGTGTCACAATTGTTAACAGTTCCGACAATTTAGTCAAGAAT
CCTAAAAGTACACGTCAGTG

Downstream 100 bases:

>100_bases
TTTGAATCAGGGGGTGGGCGGGTGTCTGCCCCTAAGTTTTTTAAGAGTCTAAATTTAGACTCCTTTTTTTTTGGCCAATA
GTTGAGTAATTCAATATTCA

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MEVIYQYAWLIPVLPLLGAMLVGLGLISFNQTTNRLRQLNAVLIISLMGAALGLSSALLWSQLQGHPTYLRTLEWAAAGN
FHLTMGYTIDNLTSLMLVIATSVAVLVMVYTDGYMAHDPGYVRFYAYLSLFGSSMLGLVVSPNLVQIYIFWELVGMCSYL
LVGFWYDRKSAADAAQKAFVTNRVGDFGLLLGILGLFWATGSFDFQIMGDRLAELVQTGSISNFLAVLFAILVFLGPVAK
SAQFPLHVWLPDAMEVPTPISALIHAATMVAAGVFLVARMYPVFEHVPAAMNVIAFTGAFTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLGSGSVIHGMEAVVGHDPALAQDMRLMGGLRKYMPATGLTFLI
GCLAISGIPPFAGFWSKDEILGAAYASNPLLWFIGWMTAGITAFYMFRMYFSTFEGKFRGNDEKIKDKLLKAKTILLELE
SAEPTPVFGPGAMKKGELAATGGHHDGHGHHSSSPHESPWTMTLPLLILAIPSMLIGLVGTPYNNYFERFIFSPTESLAE
VLEKAAEFDPNEFYIMAGGSVGVSLIGITLASLMYLQRKIDPAAIAAKIKPLYELSLNKWYFDDIYHRVFVLGLRRLARQ
VMEVDFRVVDGAVNLTGFFTLVSGEGLKYLENGRVQFYALIVFGAVLGLVIVFGVT

Sequences:

>Translated_696_residues
MEVIYQYAWLIPVLPLLGAMLVGLGLISFNQTTNRLRQLNAVLIISLMGAALGLSSALLWSQLQGHPTYLRTLEWAAAGN
FHLTMGYTIDNLTSLMLVIATSVAVLVMVYTDGYMAHDPGYVRFYAYLSLFGSSMLGLVVSPNLVQIYIFWELVGMCSYL
LVGFWYDRKSAADAAQKAFVTNRVGDFGLLLGILGLFWATGSFDFQIMGDRLAELVQTGSISNFLAVLFAILVFLGPVAK
SAQFPLHVWLPDAMEVPTPISALIHAATMVAAGVFLVARMYPVFEHVPAAMNVIAFTGAFTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLGSGSVIHGMEAVVGHDPALAQDMRLMGGLRKYMPATGLTFLI
GCLAISGIPPFAGFWSKDEILGAAYASNPLLWFIGWMTAGITAFYMFRMYFSTFEGKFRGNDEKIKDKLLKAKTILLELE
SAEPTPVFGPGAMKKGELAATGGHHDGHGHHSSSPHESPWTMTLPLLILAIPSMLIGLVGTPYNNYFERFIFSPTESLAE
VLEKAAEFDPNEFYIMAGGSVGVSLIGITLASLMYLQRKIDPAAIAAKIKPLYELSLNKWYFDDIYHRVFVLGLRRLARQ
VMEVDFRVVDGAVNLTGFFTLVSGEGLKYLENGRVQFYALIVFGAVLGLVIVFGVT
>Mature_696_residues
MEVIYQYAWLIPVLPLLGAMLVGLGLISFNQTTNRLRQLNAVLIISLMGAALGLSSALLWSQLQGHPTYLRTLEWAAAGN
FHLTMGYTIDNLTSLMLVIATSVAVLVMVYTDGYMAHDPGYVRFYAYLSLFGSSMLGLVVSPNLVQIYIFWELVGMCSYL
LVGFWYDRKSAADAAQKAFVTNRVGDFGLLLGILGLFWATGSFDFQIMGDRLAELVQTGSISNFLAVLFAILVFLGPVAK
SAQFPLHVWLPDAMEVPTPISALIHAATMVAAGVFLVARMYPVFEHVPAAMNVIAFTGAFTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLGSGSVIHGMEAVVGHDPALAQDMRLMGGLRKYMPATGLTFLI
GCLAISGIPPFAGFWSKDEILGAAYASNPLLWFIGWMTAGITAFYMFRMYFSTFEGKFRGNDEKIKDKLLKAKTILLELE
SAEPTPVFGPGAMKKGELAATGGHHDGHGHHSSSPHESPWTMTLPLLILAIPSMLIGLVGTPYNNYFERFIFSPTESLAE
VLEKAAEFDPNEFYIMAGGSVGVSLIGITLASLMYLQRKIDPAAIAAKIKPLYELSLNKWYFDDIYHRVFVLGLRRLARQ
VMEVDFRVVDGAVNLTGFFTLVSGEGLKYLENGRVQFYALIVFGAVLGLVIVFGVT

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=388, Percent_Identity=39.1752577319588, Blast_Score=258, Evalue=2e-68,
Organism=Homo sapiens, GI251831116, Length=378, Percent_Identity=21.957671957672, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788614, Length=678, Percent_Identity=34.8082595870207, Blast_Score=316, Evalue=4e-87,
Organism=Escherichia coli, GI1788829, Length=365, Percent_Identity=32.6027397260274, Blast_Score=172, Evalue=1e-43,
Organism=Escherichia coli, GI1788827, Length=450, Percent_Identity=28.6666666666667, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788831, Length=359, Percent_Identity=29.5264623955432, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788613, Length=369, Percent_Identity=26.8292682926829, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI2367154, Length=352, Percent_Identity=24.7159090909091, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI145693160, Length=322, Percent_Identity=25.776397515528, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 76014; Mature: 76014

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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EECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]