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The map label for this gene is ybiK [C]

Identifier: 17231414

GI number: 17231414

Start: 4729986

End: 4730942

Strand: Reverse

Name: ybiK [C]

Synonym: all3922

Alternate gene names: 17231414

Gene position: 4730942-4729986 (Counterclockwise)

Preceding gene: 17231415

Following gene: 17231410

Centisome position: 73.76

GC content: 46.19

Gene sequence:

>957_bases
ATGAAGTCACAGGTGCAACCAAAATTAATTATTCATGGGGGAGCAGGTAGTTCTCTCCACGGCAAAGGTGGATTAGAAGC
AGTGCGCCAAACGCTTCATGCAGTAGTAGAGGAAGTCTACGCTCTATTATTGTCAGGAGTGAATGCTTCTGTGGCAGTGG
TACGCGGTTGTCAACTGTTGGAAGATGAACCCCGCTTCAATGCTGGTACTGGTTCGGTGCTGCAATCTGATGGGCAAATC
CGCATGAGTGCTTCCATCATGGATGGTGCATTAGGACGCTTTAGTGGTGTAATAAATGTTTCACGGGTAAAAAATCCGAT
TGAGTTAGCACAATTTTTACAAAATTCTCCAGACAGAGTGCTGTCAGATTATGGTTCGGCTGAATTAGCCAGGGAAATGC
AAATCCCTAGTTACAATGCTTTAACTGAGCTGCGATTACAAGAATGGATACAAGAACGGCAAGATAATTTTAAAAGAACA
ATGGCTGGGGTAATAGCCGAACCAGAACTATTGGAAACTAGTAATGCTGGGCGCGGTACAATCGGTGTAGTAGCTTTAGA
TACCTATGGTAAATTAGCAGTAGGTACTTCTACAGGTGGCAAAGGCTTTGAGCGCATTGGGCGCGTCAGTGATTCTGCTA
TGCCAGCAGGTAATTATGCTACTAGTTATGCCGCAGTCAGTTGTACTGGCATCGGTGAAGACATTATTGATGAGTGCCTA
GCCCCCAAGATTGTCATTCGTGTCACAGATGGATTGTCCCTCCAAGACTCTATGCAGCGATCCTTTGCCGAAGCACACGA
CAACAAAAGAGATTTCGGAGCGATCGCCTTAGATGCCAATGGTGCGATCGCCTGGGGTAAAACTTGTGACATCATCCTCG
CCGCCTTCCATGATGGCGAAAAGATTGGCGATACATTAGAACTTGCGGTTGGTACGCAGGTGGGTAGTATCAGTTAG

Upstream 100 bases:

>100_bases
CGCCGTCGCCGTTCTGAGGCTAAAACCGGGGATTGAATCAGTTGACAGTTAACTGACACACCCAATATTCAAACTAAAAC
GCAAATAGCACAGGGTATAA

Downstream 100 bases:

>100_bases
GGACTGGGGATTGGGGACTGGGCGAAAATCTTTAAGTCAGTTAGGGATTGGGGACTGGGGACTGGGCGAAAATCCTGAAG
TAATTTCTCCCCCTGCTCCC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAVVEEVYALLLSGVNASVAVVRGCQLLEDEPRFNAGTGSVLQSDGQI
RMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRVLSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRT
MAGVIAEPELLETSNAGRGTIGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
APKIVIRVTDGLSLQDSMQRSFAEAHDNKRDFGAIALDANGAIAWGKTCDIILAAFHDGEKIGDTLELAVGTQVGSIS

Sequences:

>Translated_318_residues
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAVVEEVYALLLSGVNASVAVVRGCQLLEDEPRFNAGTGSVLQSDGQI
RMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRVLSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRT
MAGVIAEPELLETSNAGRGTIGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
APKIVIRVTDGLSLQDSMQRSFAEAHDNKRDFGAIALDANGAIAWGKTCDIILAAFHDGEKIGDTLELAVGTQVGSIS
>Mature_318_residues
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAVVEEVYALLLSGVNASVAVVRGCQLLEDEPRFNAGTGSVLQSDGQI
RMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRVLSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRT
MAGVIAEPELLETSNAGRGTIGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
APKIVIRVTDGLSLQDSMQRSFAEAHDNKRDFGAIALDANGAIAWGKTCDIILAAFHDGEKIGDTLELAVGTQVGSIS

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L- asparagin

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family

Homologues:

Organism=Homo sapiens, GI145275202, Length=274, Percent_Identity=33.2116788321168, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI145275200, Length=274, Percent_Identity=33.2116788321168, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787050, Length=288, Percent_Identity=34.0277777777778, Blast_Score=99, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18921183, Length=312, Percent_Identity=30.1282051282051, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357893, Length=241, Percent_Identity=30.2904564315353, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASGX_NOSS1 (Q8YQB1)

Other databases:

- EMBL:   BA000019
- PIR:   AC2296
- RefSeq:   NP_487962.1
- HSSP:   P37595
- ProteinModelPortal:   Q8YQB1
- SMR:   Q8YQB1
- STRING:   Q8YQB1
- MEROPS:   T02.002
- GeneID:   1107520
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all3922
- NMPDR:   fig|103690.1.peg.4229
- eggNOG:   COG1446
- HOGENOM:   HBG735787
- OMA:   RVKNPIE
- PhylomeDB:   Q8YQB1
- ProtClustDB:   CLSK893289
- BioCyc:   NSP103690:ALL3922-MONOMER
- InterPro:   IPR000246
- PANTHER:   PTHR10188

Pfam domain/function: PF01112 Asparaginase_2

EC number: =3.4.19.5

Molecular weight: Translated: 33638; Mature: 33638

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAVVEEVYALLLSGVNASVAVVRGCQLL
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EDEPRFNAGTGSVLQSDGQIRMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRV
CCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHHHCCEEHHHCCCHHHHHHHHHCCHHHH
LSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRTMAGVIAEPELLETSNAGRGT
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCE
IGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
EEEEEEECCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCHHCEEEEEECCCCHHHHHHHC
APKIVIRVTDGLSLQDSMQRSFAEAHDNKRDFGAIALDANGAIAWGKTCDIILAAFHDGE
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCEEEEEEEECCCC
KIGDTLELAVGTQVGSIS
HHCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAVVEEVYALLLSGVNASVAVVRGCQLL
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EDEPRFNAGTGSVLQSDGQIRMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRV
CCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHHHCCEEHHHCCCHHHHHHHHHCCHHHH
LSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRTMAGVIAEPELLETSNAGRGT
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCE
IGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
EEEEEEECCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCHHCEEEEEECCCCHHHHHHHC
APKIVIRVTDGLSLQDSMQRSFAEAHDNKRDFGAIALDANGAIAWGKTCDIILAAFHDGE
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCEEEEEEEECCCC
KIGDTLELAVGTQVGSIS
HHCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840