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The map label for this gene is ispF [H]

Identifier: 17231375

GI number: 17231375

Start: 4684073

End: 4684570

Strand: Direct

Name: ispF [H]

Synonym: alr3883

Alternate gene names: 17231375

Gene position: 4684073-4684570 (Clockwise)

Preceding gene: 17231374

Following gene: 17231376

Centisome position: 73.03

GC content: 42.77

Gene sequence:

>498_bases
ATGACTATGAGTATAAGAATTGGTAACGGCTACGATATTCACAGATTAGTGAGCGATCGCGCTTTGATTTTGGGTGGTGT
CCACATTCCCCATGAACTTGGTTTATTGGGGCATAGTGATGCTGATGTCCTCACTCACGCCATTATGGATGCCATGTTGG
GGGCATTATCTTTGGGGGATATTGGTCATTATTTCCCCCCCACTGATCCCCAATGGGCTGGGGCAGATAGTTTAGTTTTG
TTAAGTCAGGTAAATGAGTTAATCCGCACTCAAGGTTGGCGTATAGGTAACATTGACTCGGTAGTAGTTGCAGAACGTCC
AAAATTGAAACCACACATTAAAACCATGCGTGACAAACTAGCCGATATTTTGCAATTAGAACCAAATCAAATTGGTGTCA
AAGCTACAACTAATGAGAAATTAGGGCCTGTGGGACGAGAAGAGGGTATTTGTGCTTATGCTGTTGTCTTGCTGGTAAGT
TCTGATGAGATTAGTTAA

Upstream 100 bases:

>100_bases
AAATGGCAACAGGAGAAGAAGCAGGGGAGCAGGGAGCAGGGAGCAAGGGAAGCAGGGGGAGTAGGGGGAGAAATAACAAC
TGACGACTGACAACTGACTA

Downstream 100 bases:

>100_bases
CTCAAAATTGGAGGATGGAGAATGCAGGCAAAAATGCAACATAGCTAAAATATTAAATCAACTATTAGTGCATCTAAATT
TATGCCATATACAAATAATC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTMSIRIGNGYDIHRLVSDRALILGGVHIPHELGLLGHSDADVLTHAIMDAMLGALSLGDIGHYFPPTDPQWAGADSLVL
LSQVNELIRTQGWRIGNIDSVVVAERPKLKPHIKTMRDKLADILQLEPNQIGVKATTNEKLGPVGREEGICAYAVVLLVS
SDEIS

Sequences:

>Translated_165_residues
MTMSIRIGNGYDIHRLVSDRALILGGVHIPHELGLLGHSDADVLTHAIMDAMLGALSLGDIGHYFPPTDPQWAGADSLVL
LSQVNELIRTQGWRIGNIDSVVVAERPKLKPHIKTMRDKLADILQLEPNQIGVKATTNEKLGPVGREEGICAYAVVLLVS
SDEIS
>Mature_164_residues
TMSIRIGNGYDIHRLVSDRALILGGVHIPHELGLLGHSDADVLTHAIMDAMLGALSLGDIGHYFPPTDPQWAGADSLVLL
SQVNELIRTQGWRIGNIDSVVVAERPKLKPHIKTMRDKLADILQLEPNQIGVKATTNEKLGPVGREEGICAYAVVLLVSS
DEIS

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=49.0445859872611, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17874; Mature: 17743

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSIRIGNGYDIHRLVSDRALILGGVHIPHELGLLGHSDADVLTHAIMDAMLGALSLGD
CEEEEEECCCCCHHHHHHCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
IGHYFPPTDPQWAGADSLVLLSQVNELIRTQGWRIGNIDSVVVAERPKLKPHIKTMRDKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHH
ADILQLEPNQIGVKATTNEKLGPVGREEGICAYAVVLLVSSDEIS
HHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMSIRIGNGYDIHRLVSDRALILGGVHIPHELGLLGHSDADVLTHAIMDAMLGALSLGD
EEEEEECCCCCHHHHHHCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
IGHYFPPTDPQWAGADSLVLLSQVNELIRTQGWRIGNIDSVVVAERPKLKPHIKTMRDKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHH
ADILQLEPNQIGVKATTNEKLGPVGREEGICAYAVVLLVSSDEIS
HHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA