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The map label for this gene is trmD

Identifier: 17231374

GI number: 17231374

Start: 4683304

End: 4684062

Strand: Direct

Name: trmD

Synonym: alr3882

Alternate gene names: 17231374

Gene position: 4683304-4684062 (Clockwise)

Preceding gene: 17231373

Following gene: 17231375

Centisome position: 73.02

GC content: 45.59

Gene sequence:

>759_bases
GTGCGTTTTGATATAGTTACACTTTTTCCTGACTGTTTTACCTCAGTTCTTAGCTCTGGCCTGCTGGGTAAAGCTCTTGC
CAAACAGATTGCCCAAGTGAATTTGGTAAATCCCAGAGATTTCACTACAGATAAACACCGCAAGGTAGATGACGAACCTT
ATGGTGGCGGTGTAGGGATGTTGATGAAACCAGAACCAATTTTTAGTGCTGTAGAGTCGCTGCCGATTTTAGAGCGCCGA
GAAGTTATTTTGATGAGTCCCCAAGGTCAAACAATTAATCAGCCGCTATTACGAGAATTGGTCAGCAATTATGAGCAATT
AGTAGTAATTTGTGGGCATTACGAAGGGGTAGATGATAGGGTATTACATTTAGTCACCCGTGAAGTATCTTTGGGAGATT
TCATCCTCACAGGGGGAGAAATTCCGGCAATGGCATTAATTAATGGTGTGGTGCGATTGTTACCTGGAACTGTCGCCAAA
ACAGAATCCCTGACTGCGGAAAGTTTTGAGGAAGGTTTATTAGATTATCCCCAGTACACCCGTCCGGCAAACTTTCGCGG
CTGGAAAGTCCCTGATGTTTTGTTATCTGGAAATCACGCCGCTATTGCCCAATGGCGCTATGAACAACAGATTAAAAGAA
CAAGCGATCGCCGCCCTGATTTGCTAGAAAAATGGCAACAGGAGAAGAAGCAGGGGAGCAGGGAGCAGGGAGCAAGGGAA
GCAGGGGGAGTAGGGGGAGAAATAACAACTGACGACTGA

Upstream 100 bases:

>100_bases
ATACTATTGTGAAAGCTCATCTGTACTTTTACCCTGTGTAAGCATCTTGTGTTTGATAGTCATTCAAATCTAAGATTAGT
GTTCTCGAATACGAATTACT

Downstream 100 bases:

>100_bases
CAACTGACTAATGACTATGAGTATAAGAATTGGTAACGGCTACGATATTCACAGATTAGTGAGCGATCGCGCTTTGATTT
TGGGTGGTGTCCACATTCCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRFDIVTLFPDCFTSVLSSGLLGKALAKQIAQVNLVNPRDFTTDKHRKVDDEPYGGGVGMLMKPEPIFSAVESLPILERR
EVILMSPQGQTINQPLLRELVSNYEQLVVICGHYEGVDDRVLHLVTREVSLGDFILTGGEIPAMALINGVVRLLPGTVAK
TESLTAESFEEGLLDYPQYTRPANFRGWKVPDVLLSGNHAAIAQWRYEQQIKRTSDRRPDLLEKWQQEKKQGSREQGARE
AGGVGGEITTDD

Sequences:

>Translated_252_residues
MRFDIVTLFPDCFTSVLSSGLLGKALAKQIAQVNLVNPRDFTTDKHRKVDDEPYGGGVGMLMKPEPIFSAVESLPILERR
EVILMSPQGQTINQPLLRELVSNYEQLVVICGHYEGVDDRVLHLVTREVSLGDFILTGGEIPAMALINGVVRLLPGTVAK
TESLTAESFEEGLLDYPQYTRPANFRGWKVPDVLLSGNHAAIAQWRYEQQIKRTSDRRPDLLEKWQQEKKQGSREQGARE
AGGVGGEITTDD
>Mature_252_residues
MRFDIVTLFPDCFTSVLSSGLLGKALAKQIAQVNLVNPRDFTTDKHRKVDDEPYGGGVGMLMKPEPIFSAVESLPILERR
EVILMSPQGQTINQPLLRELVSNYEQLVVICGHYEGVDDRVLHLVTREVSLGDFILTGGEIPAMALINGVVRLLPGTVAK
TESLTAESFEEGLLDYPQYTRPANFRGWKVPDVLLSGNHAAIAQWRYEQQIKRTSDRRPDLLEKWQQEKKQGSREQGARE
AGGVGGEITTDD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=227, Percent_Identity=45.8149779735683, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_NOSS1 (Q8YQF1)

Other databases:

- EMBL:   BA000019
- PIR:   AC2291
- RefSeq:   NP_487922.1
- ProteinModelPortal:   Q8YQF1
- SMR:   Q8YQF1
- STRING:   Q8YQF1
- GeneID:   1107480
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr3882
- NMPDR:   fig|103690.1.peg.4189
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q8YQF1
- ProtClustDB:   PRK00026
- BioCyc:   NSP103690:ALR3882-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28046; Mature: 28046

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDIVTLFPDCFTSVLSSGLLGKALAKQIAQVNLVNPRDFTTDKHRKVDDEPYGGGVGM
CCEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCE
LMKPEPIFSAVESLPILERREVILMSPQGQTINQPLLRELVSNYEQLVVICGHYEGVDDR
EECCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHCHHHEEEEECCCCCCHHH
VLHLVTREVSLGDFILTGGEIPAMALINGVVRLLPGTVAKTESLTAESFEEGLLDYPQYT
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
RPANFRGWKVPDVLLSGNHAAIAQWRYEQQIKRTSDRRPDLLEKWQQEKKQGSREQGARE
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCHHH
AGGVGGEITTDD
HCCCCCEECCCC
>Mature Secondary Structure
MRFDIVTLFPDCFTSVLSSGLLGKALAKQIAQVNLVNPRDFTTDKHRKVDDEPYGGGVGM
CCEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCE
LMKPEPIFSAVESLPILERREVILMSPQGQTINQPLLRELVSNYEQLVVICGHYEGVDDR
EECCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHCHHHEEEEECCCCCCHHH
VLHLVTREVSLGDFILTGGEIPAMALINGVVRLLPGTVAKTESLTAESFEEGLLDYPQYT
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
RPANFRGWKVPDVLLSGNHAAIAQWRYEQQIKRTSDRRPDLLEKWQQEKKQGSREQGARE
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCHHH
AGGVGGEITTDD
HCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840