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The map label for this gene is sigE [H]

Identifier: 17231292

GI number: 17231292

Start: 4592103

End: 4593086

Strand: Direct

Name: sigE [H]

Synonym: alr3800

Alternate gene names: 17231292

Gene position: 4592103-4593086 (Clockwise)

Preceding gene: 17231291

Following gene: 17231293

Centisome position: 71.6

GC content: 47.15

Gene sequence:

>984_bases
ATGCCTACTGTTAACACCCACACAGAAGACACAAACACCAAATTCACGGCTGATATGGTGCGAACCTATCTGCGCGAAAT
TGGTCGTGTACCCCTGCTCACCCGTGAGCAAGAGATTGTTTACGGGAAGCAGGTGCAGCAAATGATGTCGCTGATTGACG
CTAAAGAGGCTTTAGCGAAAAAGTTGGATCGGGAACCGAGTTTACCAGAGTGGGCTGACCACGTTCAAAAGTCTGAAACA
GAGGTGAAACAGACTGTAACTCAGGGTAAGCGAGCCAAGCAAAAAATGATTGAAGCGAACCTGCGTTTGGTAGTGGCGAT
TGCCAAGAAATACCAAAAGCGGAATATGGAGTTCCTGGATTTAATCCAAGAAGGAACTCTAGGACTAGAAAGAGGCGTAG
AGAAATTTGACCCAATGAGGGGTTATAAATTTTCAACCTACGCTTACTGGTGGATTCGCCAAGCGATTACCCGTGCGATC
GCTCAACAAGGTCGGACTATCAGGCTACCTATCCATATTACCGAGAAACTGAACAAAATCAAAAAAGTTCAGCGCGAACT
GGCTCAAACTTTGGGGCGTTCTCCATCACCGGCAGAAATCGCCAAAGAACTAGAACTAGAACCTGCTCAGATTCGTGAGT
ATCTAAACATGGCGCGTCAACCAGTTTCTCTAGATGTGAAAGTTGGTGACAACCAGGATACAGAACTGCAAGAAATGCTC
GAAGATGATGGGCCATCCCCTGAGTATTACACCACTCAAGAATTCTTGCGCCAAGACCTGAATACCCTCTTGGCAGAACT
CACACCCCAACAGCGAGAAGTCGTAGCCTTACGCTTTGGTTTGTTAGACGGTAACGAATTATCCTTGGCGAAAGTCGGTG
AACGATTGAACCTCAGTCGTGAACGCGTCCGCCAATTAGAACATCAAGCACTAGCTCATCTGCGTCGCCGTCGAGCCAAT
GTTAAAGAATACGTCGCTAGCTAA

Upstream 100 bases:

>100_bases
TTTGGTAGCGCCTTGAGTTCTGTGAGATGCGGACGATGTTTACTAGCTAAATTTGTTACTCAATATTGATTGTGTTTACG
GAGTAGAGGACAACGCACCA

Downstream 100 bases:

>100_bases
ATTATCAAGACGTAGCGGTGCTACGTCTGTGCAAATCCCAGCGCCTCCTGAACTCACTCAGGGGGCGATTTTTTTTATGG
GTGTGTGGGTTTAGGGGTTT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPTVNTHTEDTNTKFTADMVRTYLREIGRVPLLTREQEIVYGKQVQQMMSLIDAKEALAKKLDREPSLPEWADHVQKSET
EVKQTVTQGKRAKQKMIEANLRLVVAIAKKYQKRNMEFLDLIQEGTLGLERGVEKFDPMRGYKFSTYAYWWIRQAITRAI
AQQGRTIRLPIHITEKLNKIKKVQRELAQTLGRSPSPAEIAKELELEPAQIREYLNMARQPVSLDVKVGDNQDTELQEML
EDDGPSPEYYTTQEFLRQDLNTLLAELTPQQREVVALRFGLLDGNELSLAKVGERLNLSRERVRQLEHQALAHLRRRRAN
VKEYVAS

Sequences:

>Translated_327_residues
MPTVNTHTEDTNTKFTADMVRTYLREIGRVPLLTREQEIVYGKQVQQMMSLIDAKEALAKKLDREPSLPEWADHVQKSET
EVKQTVTQGKRAKQKMIEANLRLVVAIAKKYQKRNMEFLDLIQEGTLGLERGVEKFDPMRGYKFSTYAYWWIRQAITRAI
AQQGRTIRLPIHITEKLNKIKKVQRELAQTLGRSPSPAEIAKELELEPAQIREYLNMARQPVSLDVKVGDNQDTELQEML
EDDGPSPEYYTTQEFLRQDLNTLLAELTPQQREVVALRFGLLDGNELSLAKVGERLNLSRERVRQLEHQALAHLRRRRAN
VKEYVAS
>Mature_326_residues
PTVNTHTEDTNTKFTADMVRTYLREIGRVPLLTREQEIVYGKQVQQMMSLIDAKEALAKKLDREPSLPEWADHVQKSETE
VKQTVTQGKRAKQKMIEANLRLVVAIAKKYQKRNMEFLDLIQEGTLGLERGVEKFDPMRGYKFSTYAYWWIRQAITRAIA
QQGRTIRLPIHITEKLNKIKKVQRELAQTLGRSPSPAEIAKELELEPAQIREYLNMARQPVSLDVKVGDNQDTELQEMLE
DDGPSPEYYTTQEFLRQDLNTLLAELTPQQREVVALRFGLLDGNELSLAKVGERLNLSRERVRQLEHQALAHLRRRRANV
KEYVAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=46.2882096069869, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789098, Length=304, Percent_Identity=39.4736842105263, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789871, Length=234, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788231, Length=209, Percent_Identity=27.2727272727273, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37866; Mature: 37735

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00715 SIGMA70_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVNTHTEDTNTKFTADMVRTYLREIGRVPLLTREQEIVYGKQVQQMMSLIDAKEALAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLDREPSLPEWADHVQKSETEVKQTVTQGKRAKQKMIEANLRLVVAIAKKYQKRNMEFLD
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LIQEGTLGLERGVEKFDPMRGYKFSTYAYWWIRQAITRAIAQQGRTIRLPIHITEKLNKI
HHHHCCCCHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
KKVQRELAQTLGRSPSPAEIAKELELEPAQIREYLNMARQPVSLDVKVGDNQDTELQEML
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
EDDGPSPEYYTTQEFLRQDLNTLLAELTPQQREVVALRFGLLDGNELSLAKVGERLNLSR
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
ERVRQLEHQALAHLRRRRANVKEYVAS
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTVNTHTEDTNTKFTADMVRTYLREIGRVPLLTREQEIVYGKQVQQMMSLIDAKEALAK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLDREPSLPEWADHVQKSETEVKQTVTQGKRAKQKMIEANLRLVVAIAKKYQKRNMEFLD
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LIQEGTLGLERGVEKFDPMRGYKFSTYAYWWIRQAITRAIAQQGRTIRLPIHITEKLNKI
HHHHCCCCHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
KKVQRELAQTLGRSPSPAEIAKELELEPAQIREYLNMARQPVSLDVKVGDNQDTELQEML
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
EDDGPSPEYYTTQEFLRQDLNTLLAELTPQQREVVALRFGLLDGNELSLAKVGERLNLSR
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
ERVRQLEHQALAHLRRRRANVKEYVAS
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]