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The map label for this gene is appA [H]

Identifier: 17231254

GI number: 17231254

Start: 4547801

End: 4549567

Strand: Direct

Name: appA [H]

Synonym: alr3762

Alternate gene names: 17231254

Gene position: 4547801-4549567 (Clockwise)

Preceding gene: 17231253

Following gene: 17231255

Centisome position: 70.91

GC content: 41.09

Gene sequence:

>1767_bases
ATGGGTATCTTGAATAAATTTTGTCAGGGTCAAATTTTTACTTGGTCGTTACTCAATATCTTTTTGCTTGCTGCTTGTTC
TTCTCCTCAGGGGGAAACTCCTACAAACTCTACACCCGTAACAAACTCTAGTGCAGGGGAAACCTTACGTCTACTCTATT
GGCAAGCACCAACTATACTCAATCCTCACCTAGCACAAGGATCTAAAGACTTTGAAGCCAGTCGTATCGTCTATGAACCC
CTGGCCAGCCATGATAAAGACGGTAAATTAGTGCTGTTTTTAGCCGCAGAGGAACCTACTCTAGAAAATGGTGGTATTGC
CAAAGATGGCAAATCAGTTACCTGGAAACTCAAGCAAGGAATCAAATGGTCTGATGGTCAACCTTTTACGGCTGCGGATG
TGGTATTTACTTACAAATTTCTCTCCAATCCGGCTGTCGGTGCTACCACCTCGGCTAATTACGAAGTCGTGAAAAGCGTC
GAAGCCATCGACGATTACACCGTCAAAGTTAATTTCCAGAGTCCTAACCCAGCTTGGTCACTACCTTTCGTGGGCTTAAA
TGGAATGATTATCCCCCGTCACATTTTCGAGAAATTTAATGGTAGTAACGCCAGGGAAGCCCCAGGGAATTTAATTCCTG
TAGGTACAGGCCCTTATAAAGTCGGAGAATTTAAACCCGGTGACACCATTATCTATGAGGCTAATTCTGTATTCCGTGAA
GCCAATAAACCTTTCTTTAAGCGAGTAGAACTCAAGGGAGGTGGTGATGCGACATCAGCCGCTAGAGCAGTACTGCAAAC
TGGAGATGTAGACTACGCTTGGAACTTGCAAGTAGAAGCCCCAATCCTCAAGCAACTAGAAGCAGCCGGTAAAGGGAGAT
TAGATATTAGTTTTGGTTCTTTTTTAGAACGGATTACTATCAATCATACCGATCCGAATAAACAAACAAAAGACGGCGAA
CGTTCCAGCACTGAATTTCCTCATCCTTTTTTCCAAGATATCAAAGTGCGTCAAGCGTTTAACTATGCAATTGATCGGGA
CACAATTAATCAGCAATTATACGGATCAAGTGGTCGTCCTGCTGCCAATATCCTATTAGCACCAGAGATTTATAACTCAC
CTAATACTAAATATGAATTTAGCCCCAAAAAAGCTATTGATTTATTAGATGAAGCTGGATGGAAAGATACAAATGGTAAT
GGCATTCGAGATAAAAACGGTATAGAGATGAATGTTTTACTTCAGACATCTGTAAATCCAGTAAGACAAAAAACTCAGGA
AATTATTAAACAAGGATTAACCTCTATTGGTGTCGGGGTGGAACTAAAAAGTATTGATGGTAGTATCTTCTTTTCTGGAG
ATCCATCGAACCCAGATACCTTAGGAAGGTTTCAAGCTGATTTACAAATGTTTAGTACAGGTAGTACGAATGTAGACCCT
GGTGCTTATATGAAAGGCTTTACTTGTAGCGAGATTTCCCAGAAAAAGAATAACTGGTCAAAATCCAATTATTCACGTTA
CTGTAATCCTGAATATGACAAGCTCTGGCAACAGTCCAACACAGAATTAAATCCTGAAAAACGTCGGCAGCTATTTATTC
AGATGAATGATTTGCTATTCAAAGATATTGCCCTAATTCCCTTGATTGCCCGTGCTGATGTCAATGGCGTGAGCAATAGA
CTAGTCGGTGTAGATTTAACTCCTTGGGATACCGATACGTGGAATATTAAAGATTGGCGACAAGTCCAGCCTCTTGGTAA
CAGGTAA

Upstream 100 bases:

>100_bases
AATAGTAGTACTATTTATTACATTTAAAGCTTGAGGATTTACCGTTACTACACCTGTAATCAATCAAGCCAATAACTAAT
ACTGCTAGAGAAAATCCAAC

Downstream 100 bases:

>100_bases
TTGGAATATTCTCATTACTTATTACATCAATCTCAAGCTAGAAATTCAATTTTTTAATTTATAGCTTGAGTATAATTTAA
TCTCTGCAAAATCCTACTTA

Product: oligopeptide binding protein of ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MGILNKFCQGQIFTWSLLNIFLLAACSSPQGETPTNSTPVTNSSAGETLRLLYWQAPTILNPHLAQGSKDFEASRIVYEP
LASHDKDGKLVLFLAAEEPTLENGGIAKDGKSVTWKLKQGIKWSDGQPFTAADVVFTYKFLSNPAVGATTSANYEVVKSV
EAIDDYTVKVNFQSPNPAWSLPFVGLNGMIIPRHIFEKFNGSNAREAPGNLIPVGTGPYKVGEFKPGDTIIYEANSVFRE
ANKPFFKRVELKGGGDATSAARAVLQTGDVDYAWNLQVEAPILKQLEAAGKGRLDISFGSFLERITINHTDPNKQTKDGE
RSSTEFPHPFFQDIKVRQAFNYAIDRDTINQQLYGSSGRPAANILLAPEIYNSPNTKYEFSPKKAIDLLDEAGWKDTNGN
GIRDKNGIEMNVLLQTSVNPVRQKTQEIIKQGLTSIGVGVELKSIDGSIFFSGDPSNPDTLGRFQADLQMFSTGSTNVDP
GAYMKGFTCSEISQKKNNWSKSNYSRYCNPEYDKLWQQSNTELNPEKRRQLFIQMNDLLFKDIALIPLIARADVNGVSNR
LVGVDLTPWDTDTWNIKDWRQVQPLGNR

Sequences:

>Translated_588_residues
MGILNKFCQGQIFTWSLLNIFLLAACSSPQGETPTNSTPVTNSSAGETLRLLYWQAPTILNPHLAQGSKDFEASRIVYEP
LASHDKDGKLVLFLAAEEPTLENGGIAKDGKSVTWKLKQGIKWSDGQPFTAADVVFTYKFLSNPAVGATTSANYEVVKSV
EAIDDYTVKVNFQSPNPAWSLPFVGLNGMIIPRHIFEKFNGSNAREAPGNLIPVGTGPYKVGEFKPGDTIIYEANSVFRE
ANKPFFKRVELKGGGDATSAARAVLQTGDVDYAWNLQVEAPILKQLEAAGKGRLDISFGSFLERITINHTDPNKQTKDGE
RSSTEFPHPFFQDIKVRQAFNYAIDRDTINQQLYGSSGRPAANILLAPEIYNSPNTKYEFSPKKAIDLLDEAGWKDTNGN
GIRDKNGIEMNVLLQTSVNPVRQKTQEIIKQGLTSIGVGVELKSIDGSIFFSGDPSNPDTLGRFQADLQMFSTGSTNVDP
GAYMKGFTCSEISQKKNNWSKSNYSRYCNPEYDKLWQQSNTELNPEKRRQLFIQMNDLLFKDIALIPLIARADVNGVSNR
LVGVDLTPWDTDTWNIKDWRQVQPLGNR
>Mature_587_residues
GILNKFCQGQIFTWSLLNIFLLAACSSPQGETPTNSTPVTNSSAGETLRLLYWQAPTILNPHLAQGSKDFEASRIVYEPL
ASHDKDGKLVLFLAAEEPTLENGGIAKDGKSVTWKLKQGIKWSDGQPFTAADVVFTYKFLSNPAVGATTSANYEVVKSVE
AIDDYTVKVNFQSPNPAWSLPFVGLNGMIIPRHIFEKFNGSNAREAPGNLIPVGTGPYKVGEFKPGDTIIYEANSVFREA
NKPFFKRVELKGGGDATSAARAVLQTGDVDYAWNLQVEAPILKQLEAAGKGRLDISFGSFLERITINHTDPNKQTKDGER
SSTEFPHPFFQDIKVRQAFNYAIDRDTINQQLYGSSGRPAANILLAPEIYNSPNTKYEFSPKKAIDLLDEAGWKDTNGNG
IRDKNGIEMNVLLQTSVNPVRQKTQEIIKQGLTSIGVGVELKSIDGSIFFSGDPSNPDTLGRFQADLQMFSTGSTNVDPG
AYMKGFTCSEISQKKNNWSKSNYSRYCNPEYDKLWQQSNTELNPEKRRQLFIQMNDLLFKDIALIPLIARADVNGVSNRL
VGVDLTPWDTDTWNIKDWRQVQPLGNR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=24.4186046511628, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789887, Length=433, Percent_Identity=25.1732101616628, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787495, Length=519, Percent_Identity=23.5067437379576, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789397, Length=514, Percent_Identity=20.4280155642023, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787551, Length=489, Percent_Identity=20.6543967280164, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 65191; Mature: 65060

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILNKFCQGQIFTWSLLNIFLLAACSSPQGETPTNSTPVTNSSAGETLRLLYWQAPTIL
CCCHHHHHCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
NPHLAQGSKDFEASRIVYEPLASHDKDGKLVLFLAAEEPTLENGGIAKDGKSVTWKLKQG
CCHHHCCCCCCCCHHEEEHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCC
IKWSDGQPFTAADVVFTYKFLSNPAVGATTSANYEVVKSVEAIDDYTVKVNFQSPNPAWS
CCCCCCCCCCHHHHEEEEHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEE
LPFVGLNGMIIPRHIFEKFNGSNAREAPGNLIPVGTGPYKVGEFKPGDTIIYEANSVFRE
CCEECCCCEEECHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCCCCCEEEECCHHHHHH
ANKPFFKRVELKGGGDATSAARAVLQTGDVDYAWNLQVEAPILKQLEAAGKGRLDISFGS
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEHHH
FLERITINHTDPNKQTKDGERSSTEFPHPFFQDIKVRQAFNYAIDRDTINQQLYGSSGRP
HHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCC
AANILLAPEIYNSPNTKYEFSPKKAIDLLDEAGWKDTNGNGIRDKNGIEMNVLLQTSVNP
CEEEEECCHHCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCH
VRQKTQEIIKQGLTSIGVGVELKSIDGSIFFSGDPSNPDTLGRFQADLQMFSTGSTNVDP
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHEEECCCCCCCC
GAYMKGFTCSEISQKKNNWSKSNYSRYCNPEYDKLWQQSNTELNPEKRRQLFIQMNDLLF
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHEEEEHHHHHHH
KDIALIPLIARADVNGVSNRLVGVDLTPWDTDTWNIKDWRQVQPLGNR
HHHHHHHEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
GILNKFCQGQIFTWSLLNIFLLAACSSPQGETPTNSTPVTNSSAGETLRLLYWQAPTIL
CCHHHHHCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
NPHLAQGSKDFEASRIVYEPLASHDKDGKLVLFLAAEEPTLENGGIAKDGKSVTWKLKQG
CCHHHCCCCCCCCHHEEEHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCC
IKWSDGQPFTAADVVFTYKFLSNPAVGATTSANYEVVKSVEAIDDYTVKVNFQSPNPAWS
CCCCCCCCCCHHHHEEEEHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEE
LPFVGLNGMIIPRHIFEKFNGSNAREAPGNLIPVGTGPYKVGEFKPGDTIIYEANSVFRE
CCEECCCCEEECHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCCCCCEEEECCHHHHHH
ANKPFFKRVELKGGGDATSAARAVLQTGDVDYAWNLQVEAPILKQLEAAGKGRLDISFGS
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEHHH
FLERITINHTDPNKQTKDGERSSTEFPHPFFQDIKVRQAFNYAIDRDTINQQLYGSSGRP
HHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCC
AANILLAPEIYNSPNTKYEFSPKKAIDLLDEAGWKDTNGNGIRDKNGIEMNVLLQTSVNP
CEEEEECCHHCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCH
VRQKTQEIIKQGLTSIGVGVELKSIDGSIFFSGDPSNPDTLGRFQADLQMFSTGSTNVDP
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHEEECCCCCCCC
GAYMKGFTCSEISQKKNNWSKSNYSRYCNPEYDKLWQQSNTELNPEKRRQLFIQMNDLLF
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHEEEEHHHHHHH
KDIALIPLIARADVNGVSNRLVGVDLTPWDTDTWNIKDWRQVQPLGNR
HHHHHHHEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]