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The map label for this gene is aspA [H]

Identifier: 17231216

GI number: 17231216

Start: 4496747

End: 4498162

Strand: Direct

Name: aspA [H]

Synonym: alr3724

Alternate gene names: 17231216

Gene position: 4496747-4498162 (Clockwise)

Preceding gene: 17231215

Following gene: 17231217

Centisome position: 70.11

GC content: 46.33

Gene sequence:

>1416_bases
ATGACTACAAATACAGATTTTCGTATTGAACGCGATTCGATGGGCGATCGCCAAATTGCTAGTGATGTTTATTACGGTAT
TCAAACTCTCCGAGCCGTTGAAAATTTCCCCATTAGTGGTCTTAAGCCCTTACCTACTTACGTAGATGCTGGTCTTTTAA
TTAAAAAAGCTACAGCAATTGTCAATGGAGAACTAGGTTGTATTCCCCAAGATATTAGCCAAGCGATTGTGCAAGCAACC
GATGAAATTCTGGCAGGGAAGTTAAGAGATCAGTTCGTCGTTGATGTCTATCAAGCTGGTGCAGGCACATCCCACCACAT
GAACATTAACGAAGTCCTAGCAAATCGGGCTTTGGAAATTTTGGGTGCAGAAAAGGGCAATTACCAACGAGTTAGCCCTA
ATGATCATGTGAACTACGGGCAGTCTACCAACGATGTTATTCCCACAGCAATTCGCATCGGTGGCTTATTGGCACTGACC
AAAACATTACAGCCAGCATTAGAAAAAGCGATCGCCGCCTTAGAACACAAAGCTGTAGAATTTCAAGATATTGTCAAATC
TGGCAGAACCCATTTACAGGATGCCGTTCCCGTCCGCTTGGGTGATAATTTCGCCGCTTGGGCGCATATCCTCTCAGAAC
ATCAAAACCGCATTTACACTGCCTCTGGAGACTTGATGGTACTCGGTTTAGGTGGTAGTGCGGCGGGAACAGGCTTAAAC
ACCCATCCTCAATACCGCGCCCGTGTGGTGGAAGTACTGGCAGAATTACTAAACTTACCACTCCAACCCGCCCCCCATCT
CATGGCTGCCATGCAGAGTATGTCCCCATTTGTGAACGTTTCCGGGGCGATACGCAACCTAGCCCAAGACTTAGCGAAAA
TTTCCCACGACTTGAGGCTGATGGACTCCGGGCCAAAAACTGGTTTAAAAGAAATTCAACTACCCCCAGTCCAACCCGGT
TCCTCAATCATGCCAGGCAAATATAACCCCGTTATGGCAGAGATGACATCGATGGTGTGTTTCCAGGTGATGGGTTACGA
TCAGGCGATCGCTCTTGCAGCCCAAGCCGGACAATTAGAATTAAACGTGATGATGCCGTTGATTGCCTATGATTTGATCC
ACAGCATCGAAATTCTGGGGTCAACCATCGCCGCCCTTACAGAACGCTGCATTCAAGGAATTACCGCCAACAAAGAACGC
TGTTTAGCCTACGCCGAAGGCAGTTTAGCTTTAGTTACCGCCCTCAACACTCACATTGGTTATTTAAATGCCGCAGATGT
CGCCAAAGAATCCTTAAACACAGGAAAATCTCTAAGGCAGATTGTGTTAGAAAAAGGACTGATGAGCGAAGCAGAGTTAG
CCACCGTGTTAAATCTAGAAGAAATGAGTAGTATATTGCCACTCCAAACAGAGTAA

Upstream 100 bases:

>100_bases
TAGTCAATAAGTTCGACCCCCTTGACTGAATTGTTTCCCAATAGCTTTTTAGTCTACTTGCATGGCATGATCTGTATTTT
GAGATTATCCCCACAATAAG

Downstream 100 bases:

>100_bases
TTAAGGGACTGGGGACTGGGGACTGGGGATTGATTTGTCTTTAAGCTAATACGTGTATAAATTACATAACTACTAAAAAG
GACTGTCAACAGTCAACAGT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTTNTDFRIERDSMGDRQIASDVYYGIQTLRAVENFPISGLKPLPTYVDAGLLIKKATAIVNGELGCIPQDISQAIVQAT
DEILAGKLRDQFVVDVYQAGAGTSHHMNINEVLANRALEILGAEKGNYQRVSPNDHVNYGQSTNDVIPTAIRIGGLLALT
KTLQPALEKAIAALEHKAVEFQDIVKSGRTHLQDAVPVRLGDNFAAWAHILSEHQNRIYTASGDLMVLGLGGSAAGTGLN
THPQYRARVVEVLAELLNLPLQPAPHLMAAMQSMSPFVNVSGAIRNLAQDLAKISHDLRLMDSGPKTGLKEIQLPPVQPG
SSIMPGKYNPVMAEMTSMVCFQVMGYDQAIALAAQAGQLELNVMMPLIAYDLIHSIEILGSTIAALTERCIQGITANKER
CLAYAEGSLALVTALNTHIGYLNAADVAKESLNTGKSLRQIVLEKGLMSEAELATVLNLEEMSSILPLQTE

Sequences:

>Translated_471_residues
MTTNTDFRIERDSMGDRQIASDVYYGIQTLRAVENFPISGLKPLPTYVDAGLLIKKATAIVNGELGCIPQDISQAIVQAT
DEILAGKLRDQFVVDVYQAGAGTSHHMNINEVLANRALEILGAEKGNYQRVSPNDHVNYGQSTNDVIPTAIRIGGLLALT
KTLQPALEKAIAALEHKAVEFQDIVKSGRTHLQDAVPVRLGDNFAAWAHILSEHQNRIYTASGDLMVLGLGGSAAGTGLN
THPQYRARVVEVLAELLNLPLQPAPHLMAAMQSMSPFVNVSGAIRNLAQDLAKISHDLRLMDSGPKTGLKEIQLPPVQPG
SSIMPGKYNPVMAEMTSMVCFQVMGYDQAIALAAQAGQLELNVMMPLIAYDLIHSIEILGSTIAALTERCIQGITANKER
CLAYAEGSLALVTALNTHIGYLNAADVAKESLNTGKSLRQIVLEKGLMSEAELATVLNLEEMSSILPLQTE
>Mature_470_residues
TTNTDFRIERDSMGDRQIASDVYYGIQTLRAVENFPISGLKPLPTYVDAGLLIKKATAIVNGELGCIPQDISQAIVQATD
EILAGKLRDQFVVDVYQAGAGTSHHMNINEVLANRALEILGAEKGNYQRVSPNDHVNYGQSTNDVIPTAIRIGGLLALTK
TLQPALEKAIAALEHKAVEFQDIVKSGRTHLQDAVPVRLGDNFAAWAHILSEHQNRIYTASGDLMVLGLGGSAAGTGLNT
HPQYRARVVEVLAELLNLPLQPAPHLMAAMQSMSPFVNVSGAIRNLAQDLAKISHDLRLMDSGPKTGLKEIQLPPVQPGS
SIMPGKYNPVMAEMTSMVCFQVMGYDQAIALAAQAGQLELNVMMPLIAYDLIHSIEILGSTIAALTERCIQGITANKERC
LAYAEGSLALVTALNTHIGYLNAADVAKESLNTGKSLRQIVLEKGLMSEAELATVLNLEEMSSILPLQTE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=41.7204301075269, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=48.2683982683983, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1787896, Length=455, Percent_Identity=43.5164835164835, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17553882, Length=458, Percent_Identity=43.0131004366812, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565146, Length=322, Percent_Identity=41.6149068322981, Blast_Score=251, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=42.4242424242424, Blast_Score=358, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=42.0824295010846, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=42.0824295010846, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=41.2903225806452, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=40.7809110629067, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24583245, Length=448, Percent_Identity=37.7232142857143, Blast_Score=334, Evalue=7e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50719; Mature: 50588

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNTDFRIERDSMGDRQIASDVYYGIQTLRAVENFPISGLKPLPTYVDAGLLIKKATAI
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHH
VNGELGCIPQDISQAIVQATDEILAGKLRDQFVVDVYQAGAGTSHHMNINEVLANRALEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LGAEKGNYQRVSPNDHVNYGQSTNDVIPTAIRIGGLLALTKTLQPALEKAIAALEHKAVE
HCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQDIVKSGRTHLQDAVPVRLGDNFAAWAHILSEHQNRIYTASGDLMVLGLGGSAAGTGLN
HHHHHHHCHHHHHHCCCEEECCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCC
THPQYRARVVEVLAELLNLPLQPAPHLMAAMQSMSPFVNVSGAIRNLAQDLAKISHDLRL
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEE
MDSGPKTGLKEIQLPPVQPGSSIMPGKYNPVMAEMTSMVCFQVMGYDQAIALAAQAGQLE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEE
LNVMMPLIAYDLIHSIEILGSTIAALTERCIQGITANKERCLAYAEGSLALVTALNTHIG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCEEEEEEHHHHCC
YLNAADVAKESLNTGKSLRQIVLEKGLMSEAELATVLNLEEMSSILPLQTE
CCCHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTNTDFRIERDSMGDRQIASDVYYGIQTLRAVENFPISGLKPLPTYVDAGLLIKKATAI
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHH
VNGELGCIPQDISQAIVQATDEILAGKLRDQFVVDVYQAGAGTSHHMNINEVLANRALEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LGAEKGNYQRVSPNDHVNYGQSTNDVIPTAIRIGGLLALTKTLQPALEKAIAALEHKAVE
HCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQDIVKSGRTHLQDAVPVRLGDNFAAWAHILSEHQNRIYTASGDLMVLGLGGSAAGTGLN
HHHHHHHCHHHHHHCCCEEECCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCC
THPQYRARVVEVLAELLNLPLQPAPHLMAAMQSMSPFVNVSGAIRNLAQDLAKISHDLRL
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEE
MDSGPKTGLKEIQLPPVQPGSSIMPGKYNPVMAEMTSMVCFQVMGYDQAIALAAQAGQLE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEE
LNVMMPLIAYDLIHSIEILGSTIAALTERCIQGITANKERCLAYAEGSLALVTALNTHIG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCEEEEEEHHHHCC
YLNAADVAKESLNTGKSLRQIVLEKGLMSEAELATVLNLEEMSSILPLQTE
CCCHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]