Click here to switch to the map view.

The map label for this gene is avaIM

Identifier: 17231124

GI number: 17231124

Start: 4382796

End: 4384244

Strand: Reverse

Name: avaIM

Synonym: all3632

Alternate gene names: 17231124

Gene position: 4384244-4382796 (Counterclockwise)

Preceding gene: 17231125

Following gene: 17231123

Centisome position: 68.36

GC content: 33.61

Gene sequence:

>1449_bases
ATGACTTCATTTGAGCTTGAGAGTCCAATAGAAATAAAGACTGACCCGACTGATCTTGATCAAGAGAGTGATTCCTTTGT
ACAAGAAATTTCTCGATTCAATAAAGCACTTGAGCAACGTTTTAGAGATAAGATGCGATTGCATGAAAGTTTAAGTCGAA
AAATAGTTAGTTTTCAAGCTAATAAGTCAAAACCTCAGTATCGCTGGTTTAAATATAAAGAAGCTTTTTCAGTTGATTTG
GTAAATCAGTTAATATTCGAGTACGAGAAAAAATCATTTGAGAGGATTCTTGACCCCTTCGCAGGAGCAGGAACAATGCT
ATTTGCCTGTAGTGATGCCGGTATTCAAGCAGATGGTATAGAAGTGTTACCTATTGGTCAAGAGATTATTGAAGTAAGGA
AAATAATCCAGCGACAATTCCGTCGAGAAGATTTTTTGAGATTGATTGAATGGTACAAACAAAAACCTTGGAATCAGCAT
AATAATAGAAAATATCTTAATCGTTTAAGAATTACTGACGGAGCTTATCCTCCTGAAACAGAAGCATCAATAGAGAGATT
TTTATTTTCTATAGAAAAAGAGAATATTCTTGTGAAACAAGTTCTCCGTTTTGCTCTATTGTGTATTCTTGAATCTATCA
GCTATACCCGTAAAGATGGACAGTATCTACGTTGGGATAAAAGAGCATTTAGGAAAAGTGGATCAGATAAATTTGATAAA
GGTAAAATTCTGGATTTCGATGAAGCAATTACTGAGCAAATAAAATTAATTTTGAATGATTCCTTTGACTTAATAAGTAA
TACATTATTTTGTTATGGGACTCAAAGAAGTGGAATTAATTTATTTAATGCTTCATGTCTTAAAATTCTGCCTGAATTTG
AGCAAGATTTTTACGACTGTATCATTACCTCTCCACCCTATTGTAATCGTTATGACTATACACGTACATACGCTCTAGAA
TTAGCTCTATTAGGTGTGGGAGAAAGAGATATAGTACAACTTAGGCAAGATATGCTGAGTTGTACTGTTGAAAACAAAGA
AAAGTCTCTTATTCACAATTGGCAGGAAGCATTACGCATACTTGATAAACAAGAATTGTTACAAAGTATCTTGCGCTTTC
TTGAGCGAGAGCTTGAAAGAAAAAAACTTAATAATAACGGTATTCCTCGTATGATAAAAGGATATTTCTATGAAATGGCT
TGCGTTATTATAGAATGCTTTAGAGTTTTAAAAAATGGCTCACCTTTATTTATGGTAAATGATAATGTTCGCTATGCAGG
TATTGATATTTCGGTTGATTTAATTCTTTCTAATATTGCAGAAGAAATTGGTTTTAATGTGGAGAAAATTCTTGTCTTAC
CTACTGGCAAAGGTAACAGTAGCCAACAAATGGGGACACATGGAAGAAAGACACTTCGCAAATGTGTGTATGTTTGGAGA
AAACCCTAG

Upstream 100 bases:

>100_bases
TTAGTCTATAAAAAATAGGGTTCAAATCTTAAGTGATAGACGATAGTGCTTTGTCCTGATAGAATCTTAAGTTACCTCTT
TGTTACAAGAAAAATATAAA

Downstream 100 bases:

>100_bases
TGCCATATCAATATCATATTCAAAGCAATGATGATCTTGTGACTCCATATCAAGAAGTCCGAGCAGGATTTGTTGCTTTA
GCTTTAGAAAGAAATCGAAA

Product: site-specific DNA-methyltransferase (cytosine-specific)

Products: NA

Alternate protein names: M.AvaI; N(4)- cytosine-specific methyltransferase AvaI

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKMRLHESLSRKIVSFQANKSKPQYRWFKYKEAFSVDL
VNQLIFEYEKKSFERILDPFAGAGTMLFACSDAGIQADGIEVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQH
NNRKYLNRLRITDGAYPPETEASIERFLFSIEKENILVKQVLRFALLCILESISYTRKDGQYLRWDKRAFRKSGSDKFDK
GKILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINLFNASCLKILPEFEQDFYDCIITSPPYCNRYDYTRTYALE
LALLGVGERDIVQLRQDMLSCTVENKEKSLIHNWQEALRILDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEMA
CVIIECFRVLKNGSPLFMVNDNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKGNSSQQMGTHGRKTLRKCVYVWR
KP

Sequences:

>Translated_482_residues
MTSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKMRLHESLSRKIVSFQANKSKPQYRWFKYKEAFSVDL
VNQLIFEYEKKSFERILDPFAGAGTMLFACSDAGIQADGIEVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQH
NNRKYLNRLRITDGAYPPETEASIERFLFSIEKENILVKQVLRFALLCILESISYTRKDGQYLRWDKRAFRKSGSDKFDK
GKILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINLFNASCLKILPEFEQDFYDCIITSPPYCNRYDYTRTYALE
LALLGVGERDIVQLRQDMLSCTVENKEKSLIHNWQEALRILDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEMA
CVIIECFRVLKNGSPLFMVNDNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKGNSSQQMGTHGRKTLRKCVYVWR
KP
>Mature_481_residues
TSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKMRLHESLSRKIVSFQANKSKPQYRWFKYKEAFSVDLV
NQLIFEYEKKSFERILDPFAGAGTMLFACSDAGIQADGIEVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQHN
NRKYLNRLRITDGAYPPETEASIERFLFSIEKENILVKQVLRFALLCILESISYTRKDGQYLRWDKRAFRKSGSDKFDKG
KILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINLFNASCLKILPEFEQDFYDCIITSPPYCNRYDYTRTYALEL
ALLGVGERDIVQLRQDMLSCTVENKEKSLIHNWQEALRILDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEMAC
VIIECFRVLKNGSPLFMVNDNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKGNSSQQMGTHGRKTLRKCVYVWRK
P

Specific function: This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AvaI endonuclease

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTA1_ANAVA (P0A462)

Other databases:

- EMBL:   X98339
- PIR:   S72471
- ProteinModelPortal:   P0A462
- SMR:   P0A462
- REBASE:   3287
- BRENDA:   2.1.1.113
- InterPro:   IPR017985

Pfam domain/function: NA

EC number: =2.1.1.113

Molecular weight: Translated: 56580; Mature: 56449

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKMRLHESLSRKIVSFQA
CCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKSKPQYRWFKYKEAFSVDLVNQLIFEYEKKSFERILDPFAGAGTMLFACSDAGIQADGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCE
EVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQHNNRKYLNRLRITDGAYPPET
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCC
EASIERFLFSIEKENILVKQVLRFALLCILESISYTRKDGQYLRWDKRAFRKSGSDKFDK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
GKILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINLFNASCLKILPEFEQDFYDC
CCCCCHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHE
IITSPPYCNRYDYTRTYALELALLGVGERDIVQLRQDMLSCTVENKEKSLIHNWQEALRI
EECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEMACVIIECFRVLKNGSPLFMVN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKGNSSQQMGTHGRKTLRKCVYVWR
CCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHCHHHHHHHHHHHHHHC
KP
CC
>Mature Secondary Structure 
TSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKMRLHESLSRKIVSFQA
CCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKSKPQYRWFKYKEAFSVDLVNQLIFEYEKKSFERILDPFAGAGTMLFACSDAGIQADGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCE
EVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQHNNRKYLNRLRITDGAYPPET
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCC
EASIERFLFSIEKENILVKQVLRFALLCILESISYTRKDGQYLRWDKRAFRKSGSDKFDK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
GKILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINLFNASCLKILPEFEQDFYDC
CCCCCHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHE
IITSPPYCNRYDYTRTYALELALLGVGERDIVQLRQDMLSCTVENKEKSLIHNWQEALRI
EECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEMACVIIECFRVLKNGSPLFMVN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKGNSSQQMGTHGRKTLRKCVYVWR
CCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHCHHHHHHHHHHHHHHC
KP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8917312